Restriction map for
pFN21AE9405
(Linear) (Six-base) MAPSORT of: KIEE9405.seq Check: 5690 from: 1 to: 1080
KIEE9405.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 727 823 1080
Size: 2 725 96 257
Fragments arranged by size:
725 257 96 2
AleI CACnn'nnGTG
Cuts at: 0 124 356 1080
Size: 124 232 724
Fragments arranged by size:
724 232 124
AlwNI CAG_nnn'CTG
Cuts at: 0 470 1080
Size: 470 610
ApoI r'AATT_y
Cuts at: 0 427 1080
Size: 427 653
BanII G_rGCy'C
Cuts at: 0 52 307 328 521 1080
Size: 52 255 21 193 559
Fragments arranged by size:
559 255 193 52 21
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 201 1080
Size: 93 108 879
Fragments arranged by size:
879 108 93
BbvCI CC'TCA_GC
Cuts at: 0 828 1080
Size: 828 252
BciVI GTATCCnnnnn_n'
Cuts at: 0 429 1080
Size: 429 651
Bme1580I G_kGCm'C
Cuts at: 0 672 955 1080
Size: 672 283 125
Fragments arranged by size:
672 283 125
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 93 1080
Size: 93 987
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 1080
Size: 158 922
Bpu10I CC'TnA_GC
Cuts at: 0 828 1080
Size: 828 252
BsaI GGTCTCn'nnnn_
Cuts at: 0 64 1080
Size: 64 1016
BsaBI GATnn'nnATC
Cuts at: 0 205 1080
Size: 205 875
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 322 352 1080
Size: 322 30 728
Fragments arranged by size:
728 322 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1071 1080
Size: 1071 9
BseYI C'CCAG_C
Cuts at: 0 144 489 1080
Size: 144 345 591
Fragments arranged by size:
591 345 144
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 1080
Size: 569 511
BsiHKAI G_wGCw'C
Cuts at: 0 328 521 1080
Size: 328 193 559
Fragments arranged by size:
559 328 193
Bsp1286I G_dGCh'C
Cuts at: 0 52 307 328 521 672 955 1080
Size: 52 255 21 193 151 283 125
Fragments arranged by size:
283 255 193 151 125 52 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 646 1080
Size: 646 434
BsrDI GCAATG_nn'
Cuts at: 0 253 650 1080
Size: 253 397 430
Fragments arranged by size:
430 397 253
BtsI GCAGTG_nn'
Cuts at: 0 351 363 942 1080
Size: 351 12 579 138
Fragments arranged by size:
579 351 138 12
EaeI y'GGCC_r
Cuts at: 0 398 409 788 1080
Size: 398 11 379 292
Fragments arranged by size:
398 379 292 11
EarI CTCTTCn'nnn_
Cuts at: 0 509 1057 1080
Size: 509 548 23
Fragments arranged by size:
548 509 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 916 1080
Size: 916 164
EcoICRI GAG'CTC
Cuts at: 0 326 519 1080
Size: 326 193 561
Fragments arranged by size:
561 326 193
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2 158 727 823 1080
Size: 2 156 569 96 257
Fragments arranged by size:
569 257 156 96 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1012 1080
Size: 1012 68
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 744 1080
Size: 744 336
HincII GTy'rAC
Cuts at: 0 387 891 1080
Size: 387 504 189
Fragments arranged by size:
504 387 189
MscI TGG'CCA
Cuts at: 0 400 411 790 1080
Size: 400 11 379 290
Fragments arranged by size:
400 379 290 11
MslI CAynn'nnrTG
Cuts at: 0 80 124 356 376 1080
Size: 80 44 232 20 704
Fragments arranged by size:
704 232 80 44 20
MspA1I CmG'CkG
Cuts at: 0 124 884 1080
Size: 124 760 196
Fragments arranged by size:
760 196 124
NdeI CA'TA_TG
Cuts at: 0 450 580 1080
Size: 450 130 500
Fragments arranged by size:
500 450 130
PflMI CCAn_nnn'nTGG
Cuts at: 0 407 470 1080
Size: 407 63 610
Fragments arranged by size:
610 407 63
PfoI T'CCnGG_A
Cuts at: 0 421 1080
Size: 421 659
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 806 838 1080
Size: 806 32 242
Fragments arranged by size:
806 242 32
PstI C_TGCA'G
Cuts at: 0 358 1080
Size: 358 722
PvuII CAG'CTG
Cuts at: 0 124 884 1080
Size: 124 760 196
Fragments arranged by size:
760 196 124
SacI G_AGCT'C
Cuts at: 0 328 521 1080
Size: 328 193 559
Fragments arranged by size:
559 328 193
SfcI C'TryA_G
Cuts at: 0 354 1080
Size: 354 726
StyI C'CwwG_G
Cuts at: 0 866 1080
Size: 866 214
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 255 1080
Size: 255 825
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 429 461 651 683 1080
Size: 429 32 190 32 397
Fragments arranged by size:
429 397 190 32 32
Enzymes that do cut:
AcuI AleI AlwNI ApoI BanII BbsI BbvCI BciVI
Bme1580I BplI BpmI Bpu10I BsaI BsaBI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspMI BsrDI BtsI EaeI
EarI EciI EcoICRI Eco57MI EcoNI FalI HincII MscI
MslI MspA1I NdeI PflMI PfoI PsrI PstI PvuII
SacI SfcI StyI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BbeI BcgI
BclI BfrBI BglI BglII BlpI BmgBI BmrI BmtI
BpuEI BsaAI BsaHI BsaWI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI DraI DraIII DrdI EagI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII Hin4I HindIII HpaI
KasI KpnI MfeI MluI MmeI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI