Restriction map for
pFN21AE9403
(Linear) (Six-base) MAPSORT of: KIEE9403.seq Check: 3783 from: 1 to: 1482
KIEE9403.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 552 813 954 1482
Size: 114 438 261 141 528
Fragments arranged by size:
528 438 261 141 114
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 317 925 1482
Size: 317 608 557
Fragments arranged by size:
608 557 317
AlwNI CAG_nnn'CTG
Cuts at: 0 687 1482
Size: 687 795
ApaI G_GGCC'C
Cuts at: 0 536 1037 1258 1383 1482
Size: 536 501 221 125 99
Fragments arranged by size:
536 501 221 125 99
AvaI C'yCGr_G
Cuts at: 0 502 739 749 1257 1482
Size: 502 237 10 508 225
Fragments arranged by size:
508 502 237 225 10
AvrII C'CTAG_G
Cuts at: 0 1191 1482
Size: 1191 291
BanI G'GyrC_C
Cuts at: 0 64 279 513 694 744 1482
Size: 64 215 234 181 50 738
Fragments arranged by size:
738 234 215 181 64 50
BanII G_rGCy'C
Cuts at: 0 536 1037 1258 1383 1482
Size: 536 501 221 125 99
Fragments arranged by size:
536 501 221 125 99
BbeI G_GCGC'C
Cuts at: 0 68 283 517 698 748 1482
Size: 68 215 234 181 50 734
Fragments arranged by size:
734 234 215 181 68 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 1005 1482
Size: 1005 477
BbvCI CC'TCA_GC
Cuts at: 0 1102 1482
Size: 1102 380
BciVI GTATCCnnnnn_n'
Cuts at: 0 1464 1482
Size: 1464 18
BglI GCCn_nnn'nGGC
Cuts at: 0 89 115 1072 1138 1378 1482
Size: 89 26 957 66 240 104
Fragments arranged by size:
957 240 104 89 66 26
BlpI GC'TnA_GC
Cuts at: 0 598 1482
Size: 598 884
Bme1580I G_kGCm'C
Cuts at: 0 536 1037 1258 1383 1482
Size: 536 501 221 125 99
Fragments arranged by size:
536 501 221 125 99
BmgBI CAC'GTC
Cuts at: 0 924 1368 1482
Size: 924 444 114
Fragments arranged by size:
924 444 114
BmrI ACTGGGnnnn_n'
Cuts at: 0 55 1482
Size: 55 1427
Bpu10I CC'TnA_GC
Cuts at: 0 163 1102 1482
Size: 163 939 380
Fragments arranged by size:
939 380 163
BsaHI Gr'CG_yC
Cuts at: 0 65 280 514 695 745 1482
Size: 65 215 234 181 50 737
Fragments arranged by size:
737 234 215 181 65 50
BsaWI w'CCGG_w
Cuts at: 0 791 1217 1482
Size: 791 426 265
Fragments arranged by size:
791 426 265
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 290 320 781 811 1482
Size: 290 30 461 30 671
Fragments arranged by size:
671 461 290 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 1090 1222 1482
Size: 19 1071 132 260
Fragments arranged by size:
1071 260 132 19
BseYI C'CCAG_C
Cuts at: 0 153 816 1028 1246 1311 1465 1482
Size: 153 663 212 218 65 154 17
Fragments arranged by size:
663 218 212 154 153 65 17
BsiEI CG_ry'CG
Cuts at: 0 80 119 146 262 646 666 937 1482
Size: 80 39 27 116 384 20 271 545
Fragments arranged by size:
545 384 271 116 80 39 27 20
BsiHKAI G_wGCw'C
Cuts at: 0 125 1482
Size: 125 1357
Bsp1286I G_dGCh'C
Cuts at: 0 125 536 1037 1258 1383 1482
Size: 125 411 501 221 125 99
Fragments arranged by size:
501 411 221 125 125 99
BspHI T'CATG_A
Cuts at: 0 1004 1482
Size: 1004 478
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 866 1482
Size: 866 616
BsrBI CCG'CTC
Cuts at: 0 196 1482
Size: 196 1286
BsrFI r'CCGG_y
Cuts at: 0 79 392 936 1307 1428 1482
Size: 79 313 544 371 121 54
Fragments arranged by size:
544 371 313 121 79 54
BssHII G'CGCG_C
Cuts at: 0 674 952 1482
Size: 674 278 530
Fragments arranged by size:
674 530 278
BssSI C'ACGA_G
Cuts at: 0 573 1482
Size: 573 909
BstBI TT'CG_AA
Cuts at: 0 1290 1482
Size: 1290 192
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1311 1356 1482
Size: 1311 45 126
Fragments arranged by size:
1311 126 45
BstYI r'GATC_y
Cuts at: 0 444 1482
Size: 444 1038
BtgI C'CryG_G
Cuts at: 0 74 104 110 137 146 527 649 777
Size: 74 30 6 27 9 381 122 128
Cuts at: 777 1482
Size: 705
Fragments arranged by size:
705 381 128 122 74 30 27 9
6
BtsI GCAGTG_nn'
Cuts at: 0 311 1445 1482
Size: 311 1134 37
Fragments arranged by size:
1134 311 37
EaeI y'GGCC_r
Cuts at: 0 77 107 116 259 643 663 934 986
Size: 77 30 9 143 384 20 271 52
Cuts at: 986 1058 1112 1346 1370 1482
Size: 72 54 234 24 112
Fragments arranged by size:
384 271 234 143 112 77 72 54
52 30 24 20 9
EagI C'GGCC_G
Cuts at: 0 77 116 259 643 663 934 1482
Size: 77 39 143 384 20 271 548
Fragments arranged by size:
548 384 271 143 77 39 20
EarI CTCTTCn'nnn_
Cuts at: 0 496 1341 1482
Size: 496 845 141
Fragments arranged by size:
845 496 141
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 488 1482
Size: 488 994
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 552 813 954 1482
Size: 114 438 261 141 528
Fragments arranged by size:
528 438 261 141 114
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1323 1482
Size: 1323 159
EcoO109I rG'GnC_Cy
Cuts at: 0 532 533 1033 1034 1089 1254 1316 1482
Size: 532 1 500 1 55 165 62 166
Fragments arranged by size:
532 500 166 165 62 55 1 1
FseI GG_CCGG'CC
Cuts at: 0 83 1482
Size: 83 1399
FspI TGC'GCA
Cuts at: 0 982 1482
Size: 982 500
FspAI rTGC'GCAy
Cuts at: 0 982 1482
Size: 982 500
HaeII r_GCGC'y
Cuts at: 0 68 154 283 322 460 517 610 698
Size: 68 86 129 39 138 57 93 88
Cuts at: 698 748 1482
Size: 50 734
Fragments arranged by size:
734 138 129 93 88 86 68 57
50 39
KasI G'GCGC_C
Cuts at: 0 64 279 513 694 744 1482
Size: 64 215 234 181 50 738
Fragments arranged by size:
738 234 215 181 64 50
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 599 899 1364 1482
Size: 599 300 465 118
Fragments arranged by size:
599 465 300 118
MscI TGG'CCA
Cuts at: 0 1372 1482
Size: 1372 110
MslI CAynn'nnrTG
Cuts at: 0 1183 1482
Size: 1183 299
MspA1I CmG'CkG
Cuts at: 0 76 106 148 190 285 529 651 684
Size: 76 30 42 42 95 244 122 33
Cuts at: 684 816 1352 1482
Size: 132 536 130
Fragments arranged by size:
536 244 132 130 122 95 76 42
42 33 30
NaeI GCC'GGC
Cuts at: 0 81 1482
Size: 81 1401
NarI GG'CG_CC
Cuts at: 0 65 280 514 695 745 1482
Size: 65 215 234 181 50 737
Fragments arranged by size:
737 234 215 181 65 50
NgoMIV G'CCGG_C
Cuts at: 0 79 1482
Size: 79 1403
NotI GC'GGCC_GC
Cuts at: 0 259 643 1482
Size: 259 384 839
Fragments arranged by size:
839 384 259
PasI CC'CwG_GG
Cuts at: 0 711 728 1482
Size: 711 17 754
Fragments arranged by size:
754 711 17
PfoI T'CCnGG_A
Cuts at: 0 482 1482
Size: 482 1000
PspOMI G'GGCC_C
Cuts at: 0 532 1033 1254 1379 1482
Size: 532 501 221 125 103
Fragments arranged by size:
532 501 221 125 103
PstI C_TGCA'G
Cuts at: 0 306 683 1482
Size: 306 377 799
Fragments arranged by size:
799 377 306
PvuII CAG'CTG
Cuts at: 0 190 684 816 1482
Size: 190 494 132 666
Fragments arranged by size:
666 494 190 132
SacII CC_GC'GG
Cuts at: 0 77 107 149 530 652 1482
Size: 77 30 42 381 122 830
Fragments arranged by size:
830 381 122 77 42 30
SfcI C'TryA_G
Cuts at: 0 302 679 1482
Size: 302 377 803
Fragments arranged by size:
803 377 302
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 115 1138 1378 1482
Size: 115 1023 240 104
Fragments arranged by size:
1023 240 115 104
SfoI GGC'GCC
Cuts at: 0 66 281 515 696 746 1482
Size: 66 215 234 181 50 736
Fragments arranged by size:
736 234 215 181 66 50
SmaI CCC'GGG
Cuts at: 0 504 751 1259 1482
Size: 504 247 508 223
Fragments arranged by size:
508 504 247 223
StyI C'CwwG_G
Cuts at: 0 213 354 1191 1482
Size: 213 141 837 291
Fragments arranged by size:
837 291 213 141
TatI w'GTAC_w
Cuts at: 0 1398 1482
Size: 1398 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 290 322 1482
Size: 290 32 1160
Fragments arranged by size:
1160 290 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 600 1482
Size: 600 882
XmaI C'CCGG_G
Cuts at: 0 502 749 1257 1482
Size: 502 247 508 225
Fragments arranged by size:
508 502 247 225
Enzymes that do cut:
AcuI AlfI AlwNI ApaI AvaI AvrII BanI BanII
BbeI BbsI BbvCI BciVI BglI BlpI Bme1580I BmgBI
BmrI Bpu10I BsaHI BsaWI BsaXI BseRI BseYI BsiEI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BssHII BssSI
BstBI BstXI BstYI BtgI BtsI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I FseI FspI FspAI HaeII
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NotI PasI PfoI PspOMI PstI PvuII SacII SfcI
SfiI SfoI SmaI StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AloI ApaLI ApoI AscI AseI
AsiSI BaeI BamHI BcgI BclI BfrBI BglII BmtI
BplI BpmI BpuEI BsaI BsaAI BsaBI BsgI BsiWI
BsmI BsmBI BspEI BsrDI BsrGI BstAPI BstEII BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI
EcoRI EcoRV FalI Hin4I HincII HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NruI NsiI NspI
PacI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI SbfI ScaI SexAI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I
XbaI XhoI XmnI ZraI