Restriction map for
pFN21AE9402
(Linear) (Six-base) MAPSORT of: KIEE9402.seq Check: 4274 from: 1 to: 1002
KIEE9402.orf
With 182 enzymes: SgfI *
December 27, 2011 16:39 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 568 1002
Size: 568 434
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 304 625 1002
Size: 34 270 321 377
Fragments arranged by size:
377 321 270 34
AfeI AGC'GCT
Cuts at: 0 932 1002
Size: 932 70
AlwNI CAG_nnn'CTG
Cuts at: 0 118 1002
Size: 118 884
AvaI C'yCGr_G
Cuts at: 0 38 92 159 1002
Size: 38 54 67 843
Fragments arranged by size:
843 67 54 38
BanII G_rGCy'C
Cuts at: 0 125 1002
Size: 125 877
BbsI GAAGACnn'nnnn_
Cuts at: 0 14 1002
Size: 14 988
BglII A'GATC_T
Cuts at: 0 656 1002
Size: 656 346
BmgBI CAC'GTC
Cuts at: 0 719 1002
Size: 719 283
BmrI ACTGGGnnnn_n'
Cuts at: 0 986 1002
Size: 986 16
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 90 1002
Size: 90 912
Bpu10I CC'TnA_GC
Cuts at: 0 119 1002
Size: 119 883
BsaI GGTCTCn'nnnn_
Cuts at: 0 753 1002
Size: 753 249
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 78 1002
Size: 78 924
BseYI C'CCAG_C
Cuts at: 0 217 1002
Size: 217 785
BsiEI CG_ry'CG
Cuts at: 0 350 1002
Size: 350 652
BsiHKAI G_wGCw'C
Cuts at: 0 125 231 1002
Size: 125 106 771
Fragments arranged by size:
771 125 106
BsmBI CGTCTCn'nnnn_
Cuts at: 0 542 1002
Size: 542 460
Bsp1286I G_dGCh'C
Cuts at: 0 125 231 1002
Size: 125 106 771
Fragments arranged by size:
771 125 106
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 278 323 568 1002
Size: 278 45 245 434
Fragments arranged by size:
434 278 245 45
BsrBI CCG'CTC
Cuts at: 0 579 1002
Size: 579 423
BsrFI r'CCGG_y
Cuts at: 0 277 1002
Size: 277 725
BssHII G'CGCG_C
Cuts at: 0 982 1002
Size: 982 20
BstXI CCAn_nnnn'nTGG
Cuts at: 0 339 510 1002
Size: 339 171 492
Fragments arranged by size:
492 339 171
BstYI r'GATC_y
Cuts at: 0 656 920 1002
Size: 656 264 82
Fragments arranged by size:
656 264 82
DraIII CAC_nnn'GTG
Cuts at: 0 729 1002
Size: 729 273
DrdI GACnn_nn'nnGTC
Cuts at: 0 16 1002
Size: 16 986
EaeI y'GGCC_r
Cuts at: 0 275 596 713 1002
Size: 275 321 117 289
Fragments arranged by size:
321 289 275 117
EarI CTCTTCn'nnn_
Cuts at: 0 625 1002
Size: 625 377
EcoICRI GAG'CTC
Cuts at: 0 123 1002
Size: 123 879
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 304 625 1002
Size: 34 270 321 377
Fragments arranged by size:
377 321 270 34
EcoO109I rG'GnC_Cy
Cuts at: 0 497 1002
Size: 497 505
HaeII r_GCGC'y
Cuts at: 0 934 1002
Size: 934 68
HincII GTy'rAC
Cuts at: 0 132 408 1002
Size: 132 276 594
Fragments arranged by size:
594 276 132
MscI TGG'CCA
Cuts at: 0 715 1002
Size: 715 287
MspA1I CmG'CkG
Cuts at: 0 58 1002
Size: 58 944
NaeI GCC'GGC
Cuts at: 0 279 1002
Size: 279 723
NgoMIV G'CCGG_C
Cuts at: 0 277 1002
Size: 277 725
PasI CC'CwG_GG
Cuts at: 0 137 1002
Size: 137 865
PflMI CCAn_nnn'nTGG
Cuts at: 0 711 1002
Size: 711 291
PpuMI rG'GwC_Cy
Cuts at: 0 497 1002
Size: 497 505
PstI C_TGCA'G
Cuts at: 0 388 565 701 1002
Size: 388 177 136 301
Fragments arranged by size:
388 301 177 136
PvuI CG_AT'CG
Cuts at: 0 350 1002
Size: 350 652
SacI G_AGCT'C
Cuts at: 0 125 1002
Size: 125 877
SfcI C'TryA_G
Cuts at: 0 384 561 697 1002
Size: 384 177 136 305
Fragments arranged by size:
384 305 177 136
SmaI CCC'GGG
Cuts at: 0 94 1002
Size: 94 908
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 246 1002
Size: 246 756
TatI w'GTAC_w
Cuts at: 0 425 1002
Size: 425 577
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 221 253 1002
Size: 221 32 749
Fragments arranged by size:
749 221 32
Tth111I GACn'n_nGTC
Cuts at: 0 145 1002
Size: 145 857
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 564 1002
Size: 564 438
XmaI C'CCGG_G
Cuts at: 0 92 1002
Size: 92 910
Enzymes that do cut:
AarI AcuI AfeI AlwNI AvaI BanII BbsI BglII
BmgBI BmrI BplI Bpu10I BsaI BseRI BseYI BsiEI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssHII BstXI
BstYI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoO109I
HaeII HincII MscI MspA1I NaeI NgoMIV PasI PflMI
PpuMI PstI PvuI SacI SfcI SmaI TaqII TatI
TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BlpI Bme1580I BmtI BpmI
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiWI
BsmI BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI
DraI EagI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI Hin4I HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NarI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuII RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI XbaI XhoI XmnI ZraI