Restriction map for
pFN21AE9401
(Linear) (Six-base) MAPSORT of: KIEE9401.seq Check: 514 from: 1 to: 1524
KIEE9401.orf
With 182 enzymes: SgfI *
December 27, 2011 16:38 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 19 180 1524
Size: 19 161 1344
Fragments arranged by size:
1344 161 19
AatII G_ACGT'C
Cuts at: 0 7 1100 1524
Size: 7 1093 424
Fragments arranged by size:
1093 424 7
Acc65I G'GTAC_C
Cuts at: 0 1290 1524
Size: 1290 234
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 1426 1502 1524
Size: 871 555 76 22
Fragments arranged by size:
871 555 76 22
AflIII A'CryG_T
Cuts at: 0 47 1524
Size: 47 1477
AleI CACnn'nnGTG
Cuts at: 0 274 1524
Size: 274 1250
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 166 1524
Size: 166 1358
AlwNI CAG_nnn'CTG
Cuts at: 0 186 1524
Size: 186 1338
ApaI G_GGCC'C
Cuts at: 0 1241 1524
Size: 1241 283
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1274 1282 1307 1315 1524
Size: 1274 8 25 8 209
Fragments arranged by size:
1274 209 25 8 8
BanI G'GyrC_C
Cuts at: 0 460 1290 1524
Size: 460 830 234
Fragments arranged by size:
830 460 234
BanII G_rGCy'C
Cuts at: 0 1241 1524
Size: 1241 283
BbsI GAAGACnn'nnnn_
Cuts at: 0 1076 1524
Size: 1076 448
BclI T'GATC_A
Cuts at: 0 1304 1524
Size: 1304 220
Bme1580I G_kGCm'C
Cuts at: 0 1241 1524
Size: 1241 283
BmrI ACTGGGnnnn_n'
Cuts at: 0 1127 1290 1524
Size: 1127 163 234
Fragments arranged by size:
1127 234 163
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 509 1524
Size: 509 1015
BsaAI yAC'GTr
Cuts at: 0 50 1524
Size: 50 1474
BsaBI GATnn'nnATC
Cuts at: 0 1204 1524
Size: 1204 320
BsaHI Gr'CG_yC
Cuts at: 0 4 1097 1524
Size: 4 1093 427
Fragments arranged by size:
1093 427 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 394 424 1524
Size: 394 30 1100
Fragments arranged by size:
1100 394 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 208 442 482 775 925 997 1151 1524
Size: 208 234 40 293 150 72 154 373
Fragments arranged by size:
373 293 234 208 154 150 72 40
BseYI C'CCAG_C
Cuts at: 0 431 506 568 1524
Size: 431 75 62 956
Fragments arranged by size:
956 431 75 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 294 671 1050 1524
Size: 294 377 379 474
Fragments arranged by size:
474 379 377 294
BsiHKAI G_wGCw'C
Cuts at: 0 490 1524
Size: 490 1034
Bsp1286I G_dGCh'C
Cuts at: 0 490 1241 1524
Size: 490 751 283
Fragments arranged by size:
751 490 283
BspHI T'CATG_A
Cuts at: 0 620 1524
Size: 620 904
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 19 180 239 455 1466 1524
Size: 19 161 59 216 1011 58
Fragments arranged by size:
1011 216 161 59 58 19
BsrBI CCG'CTC
Cuts at: 0 101 1524
Size: 101 1423
BsrDI GCAATG_nn'
Cuts at: 0 540 1524
Size: 540 984
BsrGI T'GTAC_A
Cuts at: 0 677 1524
Size: 677 847
BssSI C'ACGA_G
Cuts at: 0 247 1524
Size: 247 1277
BstAPI GCAn_nnn'nTGC
Cuts at: 0 186 672 1524
Size: 186 486 852
Fragments arranged by size:
852 486 186
BstEII G'GTnAC_C
Cuts at: 0 192 442 1524
Size: 192 250 1082
Fragments arranged by size:
1082 250 192
BstXI CCAn_nnnn'nTGG
Cuts at: 0 81 369 1524
Size: 81 288 1155
Fragments arranged by size:
1155 288 81
BtgI C'CryG_G
Cuts at: 0 87 438 638 1524
Size: 87 351 200 886
Fragments arranged by size:
886 351 200 87
BtsI GCAGTG_nn'
Cuts at: 0 1112 1342 1524
Size: 1112 230 182
Fragments arranged by size:
1112 230 182
EaeI y'GGCC_r
Cuts at: 0 266 473 935 1262 1524
Size: 266 207 462 327 262
Fragments arranged by size:
462 327 266 262 207
EarI CTCTTCn'nnn_
Cuts at: 0 289 734 1524
Size: 289 445 790
Fragments arranged by size:
790 445 289
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 1426 1502 1524
Size: 871 555 76 22
Fragments arranged by size:
871 555 76 22
EcoO109I rG'GnC_Cy
Cuts at: 0 1237 1238 1524
Size: 1237 1 286
Fragments arranged by size:
1237 286 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1431 1524
Size: 1431 93
HaeII r_GCGC'y
Cuts at: 0 160 438 1524
Size: 160 278 1086
Fragments arranged by size:
1086 278 160
HincII GTy'rAC
Cuts at: 0 357 1023 1524
Size: 357 666 501
Fragments arranged by size:
666 501 357
KpnI G_GTAC'C
Cuts at: 0 1294 1524
Size: 1294 230
MscI TGG'CCA
Cuts at: 0 268 475 937 1524
Size: 268 207 462 587
Fragments arranged by size:
587 462 268 207
MslI CAynn'nnrTG
Cuts at: 0 52 274 367 800 1352 1524
Size: 52 222 93 433 552 172
Fragments arranged by size:
552 433 222 172 93 52
MspA1I CmG'CkG
Cuts at: 0 183 440 1524
Size: 183 257 1084
Fragments arranged by size:
1084 257 183
NspI r_CATG'y
Cuts at: 0 57 670 1371 1404 1524
Size: 57 613 701 33 120
Fragments arranged by size:
701 613 120 57 33
PasI CC'CwG_GG
Cuts at: 0 257 1233 1524
Size: 257 976 291
Fragments arranged by size:
976 291 257
PflMI CCAn_nnn'nTGG
Cuts at: 0 554 1524
Size: 554 970
PfoI T'CCnGG_A
Cuts at: 0 767 1115 1394 1524
Size: 767 348 279 130
Fragments arranged by size:
767 348 279 130
PmlI CAC'GTG
Cuts at: 0 50 1524
Size: 50 1474
PshAI GACnn'nnGTC
Cuts at: 0 1007 1524
Size: 1007 517
PspOMI G'GGCC_C
Cuts at: 0 1237 1524
Size: 1237 287
PstI C_TGCA'G
Cuts at: 0 191 239 485 1337 1524
Size: 191 48 246 852 187
Fragments arranged by size:
852 246 191 187 48
PvuII CAG'CTG
Cuts at: 0 183 1524
Size: 183 1341
SacII CC_GC'GG
Cuts at: 0 441 1524
Size: 441 1083
SfcI C'TryA_G
Cuts at: 0 187 235 481 1333 1524
Size: 187 48 246 852 191
Fragments arranged by size:
852 246 191 187 48
SphI G_CATG'C
Cuts at: 0 57 670 1524
Size: 57 613 854
Fragments arranged by size:
854 613 57
StyI C'CwwG_G
Cuts at: 0 815 836 857 1524
Size: 815 21 21 667
Fragments arranged by size:
815 667 21 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 249 346 1033 1146 1260 1290 1466 1524
Size: 249 97 687 113 114 30 176 58
Fragments arranged by size:
687 249 176 114 113 97 58 30
TatI w'GTAC_w
Cuts at: 0 677 1524
Size: 677 847
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 394 426 1524
Size: 394 32 1098
Fragments arranged by size:
1098 394 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 503 1524
Size: 503 1021
XmnI GAAnn'nnTTC
Cuts at: 0 1411 1414 1524
Size: 1411 3 110
Fragments arranged by size:
1411 110 3
ZraI GAC'GTC
Cuts at: 0 5 1098 1524
Size: 5 1093 426
Fragments arranged by size:
1093 426 5
Enzymes that do cut:
AarI AatII Acc65I AcuI AflIII AleI AlfI AlwNI
ApaI BaeI BanI BanII BbsI BclI Bme1580I BmrI
BplI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrDI BsrGI BssSI
BstAPI BstEII BstXI BtgI BtsI EaeI EarI Eco57MI
EcoO109I FalI HaeII HincII KpnI MscI MslI MspA1I
NspI PasI PflMI PfoI PmlI PshAI PspOMI PstI
PvuII SacII SfcI SphI StyI TaqII TatI TstI
XcmI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AgeI AhdI AloI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BamHI BbeI
BbvCI BcgI BciVI BfrBI BglI BglII BlpI BmgBI
BmtI BpmI Bpu10I BpuEI BsaI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BsrFI BssHII BstBI BstYI BstZ17I
Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
Hin4I HindIII HpaI KasI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PciI PmeI PpiI PpuMI PsiI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmaI