Restriction map for
pFN21AE9393
(Linear) (Six-base) MAPSORT of: KIEE9393.seq Check: 5496 from: 1 to: 3753
KIEE9393.orf
With 182 enzymes: SgfI *
December 5, 2014 13:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 681 2426 2508 3753
Size: 681 1745 82 1245
Fragments arranged by size:
1745 1245 681 82
AatII G_ACGT'C
Cuts at: 0 1586 3753
Size: 1586 2167
AccI GT'mk_AC
Cuts at: 0 3508 3753
Size: 3508 245
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 637 847 2218 2786 3110 3753
Size: 216 421 210 1371 568 324 643
Fragments arranged by size:
1371 643 568 421 324 216 210
AflII C'TTAA_G
Cuts at: 0 1183 3753
Size: 1183 2570
AhdI GACnn_n'nnGTC
Cuts at: 0 42 2118 3753
Size: 42 2076 1635
Fragments arranged by size:
2076 1635 42
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2011 2661 3753
Size: 2011 650 1092
Fragments arranged by size:
2011 1092 650
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 695 727 2281 2313 3753
Size: 695 32 1554 32 1440
Fragments arranged by size:
1554 1440 695 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 478 1961 2196 2960 3720 3753
Size: 478 1483 235 764 760 33
Fragments arranged by size:
1483 764 760 478 235 33
ApaI G_GGCC'C
Cuts at: 0 2238 3753
Size: 2238 1515
ApaLI G'TGCA_C
Cuts at: 0 850 3753
Size: 850 2903
ApoI r'AATT_y
Cuts at: 0 97 1057 3634 3753
Size: 97 960 2577 119
Fragments arranged by size:
2577 960 119 97
AvaI C'yCGr_G
Cuts at: 0 40 617 1888 3753
Size: 40 577 1271 1865
Fragments arranged by size:
1865 1271 577 40
AvrII C'CTAG_G
Cuts at: 0 2230 3753
Size: 2230 1523
BamHI G'GATC_C
Cuts at: 0 278 2405 3753
Size: 278 2127 1348
Fragments arranged by size:
2127 1348 278
BanI G'GyrC_C
Cuts at: 0 530 1293 1870 3753
Size: 530 763 577 1883
Fragments arranged by size:
1883 763 577 530
BanII G_rGCy'C
Cuts at: 0 242 429 574 1898 1946 2238 3188 3753
Size: 242 187 145 1324 48 292 950 565
Fragments arranged by size:
1324 950 565 292 242 187 145 48
BbeI G_GCGC'C
Cuts at: 0 1874 3753
Size: 1874 1879
BbsI GAAGACnn'nnnn_
Cuts at: 0 65 827 2558 2973 3248 3753
Size: 65 762 1731 415 275 505
Fragments arranged by size:
1731 762 505 415 275 65
BbvCI CC'TCA_GC
Cuts at: 0 1857 3753
Size: 1857 1896
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3083 3117 3753
Size: 3083 34 636
Fragments arranged by size:
3083 636 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1101 3753
Size: 1101 2652
BclI T'GATC_A
Cuts at: 0 2187 3753
Size: 2187 1566
BfrBI ATG'CAT
Cuts at: 0 151 2731 3753
Size: 151 2580 1022
Fragments arranged by size:
2580 1022 151
BglI GCCn_nnn'nGGC
Cuts at: 0 682 1687 1839 3753
Size: 682 1005 152 1914
Fragments arranged by size:
1914 1005 682 152
BglII A'GATC_T
Cuts at: 0 955 3753
Size: 955 2798
BlpI GC'TnA_GC
Cuts at: 0 1189 2038 3753
Size: 1189 849 1715
Fragments arranged by size:
1715 1189 849
Bme1580I G_kGCm'C
Cuts at: 0 533 854 1778 2238 3753
Size: 533 321 924 460 1515
Fragments arranged by size:
1515 924 533 460 321
BmgBI CAC'GTC
Cuts at: 0 3558 3753
Size: 3558 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 3606 3753
Size: 3606 147
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1253 1383 1413 1872 2016 3753
Size: 1253 130 30 459 144 1737
Fragments arranged by size:
1737 1253 459 144 130 30
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 980 1332 1383 1419 2137 2308 2921
Size: 10 970 352 51 36 718 171 613
Cuts at: 2921 3753
Size: 832
Fragments arranged by size:
970 832 718 613 352 171 51 36
10
Bpu10I CC'TnA_GC
Cuts at: 0 821 1857 3753
Size: 821 1036 1896
Fragments arranged by size:
1896 1036 821
BsaI GGTCTCn'nnnn_
Cuts at: 0 1378 2372 2852 2939 3473 3753
Size: 1378 994 480 87 534 280
Fragments arranged by size:
1378 994 534 480 280 87
BsaHI Gr'CG_yC
Cuts at: 0 1583 1871 3753
Size: 1583 288 1882
Fragments arranged by size:
1882 1583 288
BsaWI w'CCGG_w
Cuts at: 0 275 1369 3753
Size: 275 1094 2384
Fragments arranged by size:
2384 1094 275
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 584 614 2281 2311 3753
Size: 584 30 1667 30 1442
Fragments arranged by size:
1667 1442 584 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 961 1219 1335 1675 1906 1992 2088 2091
Size: 961 258 116 340 231 86 96 3
Cuts at: 2091 2146 2166 2305 3753
Size: 55 20 139 1448
Fragments arranged by size:
1448 961 340 258 231 139 116 96
86 55 20 3
BseYI C'CCAG_C
Cuts at: 0 526 745 1621 1914 2479 3753
Size: 526 219 876 293 565 1274
Fragments arranged by size:
1274 876 565 526 293 219
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 840 3565 3661 3706 3753
Size: 508 332 2725 96 45 47
Fragments arranged by size:
2725 508 332 96 47 45
BsiEI CG_ry'CG
Cuts at: 0 770 1733 1904 2707 2917 3753
Size: 770 963 171 803 210 836
Fragments arranged by size:
963 836 803 770 210 171
BsiHKAI G_wGCw'C
Cuts at: 0 854 1651 3188 3415 3695 3753
Size: 854 797 1537 227 280 58
Fragments arranged by size:
1537 854 797 280 227 58
Bsp1286I G_dGCh'C
Cuts at: 0 242 429 533 574 854 1651 1778 1898
Size: 242 187 104 41 280 797 127 120
Cuts at: 1898 1946 2238 3188 3415 3695 3753
Size: 48 292 950 227 280 58
Fragments arranged by size:
950 797 292 280 280 242 227 187
127 120 104 58 48 41
BspEI T'CCGG_A
Cuts at: 0 1369 3753
Size: 1369 2384
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 681 2020 2426 2508 3753
Size: 681 1339 406 82 1245
Fragments arranged by size:
1339 1245 681 406 82
BsrDI GCAATG_nn'
Cuts at: 0 642 3059 3131 3753
Size: 642 2417 72 622
Fragments arranged by size:
2417 642 622 72
BsrFI r'CCGG_y
Cuts at: 0 1452 1883 3666 3753
Size: 1452 431 1783 87
Fragments arranged by size:
1783 1452 431 87
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3729 3753
Size: 3729 24
BstEII G'GTnAC_C
Cuts at: 0 3669 3753
Size: 3669 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1594 2286 3392 3462 3753
Size: 1594 692 1106 70 291
Fragments arranged by size:
1594 1106 692 291 70
BstYI r'GATC_y
Cuts at: 0 278 331 955 1372 2405 3651 3753
Size: 278 53 624 417 1033 1246 102
Fragments arranged by size:
1246 1033 624 417 278 102 53
Bsu36I CC'TnA_GG
Cuts at: 0 2825 3753
Size: 2825 928
BtgI C'CryG_G
Cuts at: 0 677 1262 1539 1727 1733 1739 1787 1820
Size: 677 585 277 188 6 6 48 33
Cuts at: 1820 1898 1904 2159 2929 3140 3391 3461 3753
Size: 78 6 255 770 211 251 70 292
Fragments arranged by size:
770 677 585 292 277 255 251 211
188 78 70 48 33 6 6 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2259 3753
Size: 2259 1494
BtsI GCAGTG_nn'
Cuts at: 0 852 1200 1778 2229 2643 3006 3753
Size: 852 348 578 451 414 363 747
Fragments arranged by size:
852 747 578 451 414 363 348
ClaI AT'CG_AT
Cuts at: 0 398 3753
Size: 398 3355
DrdI GACnn_nn'nnGTC
Cuts at: 0 2082 2397 3753
Size: 2082 315 1356
Fragments arranged by size:
2082 1356 315
EaeI y'GGCC_r
Cuts at: 0 546 683 792 1730 1736 1901 2319 2609
Size: 546 137 109 938 6 165 418 290
Cuts at: 2609 2914 2949 3548 3605 3617 3753
Size: 305 35 599 57 12 136
Fragments arranged by size:
938 599 546 418 305 290 165 137
136 109 57 35 12 6
EagI C'GGCC_G
Cuts at: 0 1730 1901 2914 3753
Size: 1730 171 1013 839
Fragments arranged by size:
1730 1013 839 171
EarI CTCTTCn'nnn_
Cuts at: 0 235 1196 2604 2814 3753
Size: 235 961 1408 210 939
Fragments arranged by size:
1408 961 939 235 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2870 3309 3753
Size: 2870 439 444
Fragments arranged by size:
2870 444 439
EcoICRI GAG'CTC
Cuts at: 0 3186 3753
Size: 3186 567
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 10 216 637 847 980 1332 1383 1419
Size: 10 206 421 210 133 352 51 36
Cuts at: 1419 2137 2218 2308 2786 2921 3110 3753
Size: 718 81 90 478 135 189 643
Fragments arranged by size:
718 643 478 421 352 210 206 189
135 133 90 81 51 36 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 25 2422 3619 3753
Size: 25 2397 1197 134
Fragments arranged by size:
2397 1197 134 25
EcoO109I rG'GnC_Cy
Cuts at: 0 36 581 804 1689 1853 1958 2234 2235
Size: 36 545 223 885 164 105 276 1
Cuts at: 2235 3136 3223 3717 3753
Size: 901 87 494 36
Fragments arranged by size:
901 885 545 494 276 223 164 105
87 36 36 1
EcoRI G'AATT_C
Cuts at: 0 3634 3753
Size: 3634 119
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1198 3753
Size: 1198 2555
HaeII r_GCGC'y
Cuts at: 0 1262 1874 2025 2447 3497 3743 3753
Size: 1262 612 151 422 1050 246 10
Fragments arranged by size:
1262 1050 612 422 246 151 10
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 253 285 940 972 2065 2071
Size: 28 32 193 32 655 32 1093 6
Cuts at: 2071 2097 2103 2104 2136 2350 2352 2382 2384
Size: 26 6 1 32 214 2 30 2
Cuts at: 2384 3753
Size: 1369
Fragments arranged by size:
1369 1093 655 214 193 32 32 32
32 30 28 26 6 6 2 2
1
HincII GTy'rAC
Cuts at: 0 3509 3753
Size: 3509 244
KasI G'GCGC_C
Cuts at: 0 1870 3753
Size: 1870 1883
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 333 2102 2135 2969 3509 3753
Size: 296 37 1769 33 834 540 244
Fragments arranged by size:
1769 834 540 296 244 37 33
MscI TGG'CCA
Cuts at: 0 2611 3619 3753
Size: 2611 1008 134
Fragments arranged by size:
2611 1008 134
MslI CAynn'nnrTG
Cuts at: 0 3390 3753
Size: 3390 363
MspA1I CmG'CkG
Cuts at: 0 478 701 974 1331 1476 1729 1789 1822
Size: 478 223 273 357 145 253 60 33
Cuts at: 1822 1900 1906 2209 2841 3753
Size: 78 6 303 632 912
Fragments arranged by size:
912 632 478 357 303 273 253 223
145 78 60 33 6
NarI GG'CG_CC
Cuts at: 0 1871 3753
Size: 1871 1882
NcoI C'CATG_G
Cuts at: 0 677 3140 3753
Size: 677 2463 613
Fragments arranged by size:
2463 677 613
NotI GC'GGCC_GC
Cuts at: 0 1901 3753
Size: 1901 1852
NsiI A_TGCA'T
Cuts at: 0 153 2733 3753
Size: 153 2580 1020
Fragments arranged by size:
2580 1020 153
PasI CC'CwG_GG
Cuts at: 0 1693 2580 3753
Size: 1693 887 1173
Fragments arranged by size:
1693 1173 887
PflMI CCAn_nnn'nTGG
Cuts at: 0 1147 3543 3753
Size: 1147 2396 210
Fragments arranged by size:
2396 1147 210
PfoI T'CCnGG_A
Cuts at: 0 26 992 1586 2179 2401 3753
Size: 26 966 594 593 222 1352
Fragments arranged by size:
1352 966 594 593 222 26
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 424 456 2281 2313 3753
Size: 424 32 1825 32 1440
Fragments arranged by size:
1825 1440 424 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 36 581 1853 3753
Size: 36 545 1272 1900
Fragments arranged by size:
1900 1272 545 36
PshAI GACnn'nnGTC
Cuts at: 0 3514 3753
Size: 3514 239
PspOMI G'GGCC_C
Cuts at: 0 2234 3753
Size: 2234 1519
PspXI vC'TCGA_Gb
Cuts at: 0 40 3753
Size: 40 3713
PstI C_TGCA'G
Cuts at: 0 1285 2505 2846 3753
Size: 1285 1220 341 907
Fragments arranged by size:
1285 1220 907 341
PvuII CAG'CTG
Cuts at: 0 478 974 1476 2841 3753
Size: 478 496 502 1365 912
Fragments arranged by size:
1365 912 502 496 478
SacI G_AGCT'C
Cuts at: 0 3188 3753
Size: 3188 565
SacII CC_GC'GG
Cuts at: 0 1730 1790 1823 1901 1907 3753
Size: 1730 60 33 78 6 1846
Fragments arranged by size:
1846 1730 78 60 33 6
SalI G'TCGA_C
Cuts at: 0 3507 3753
Size: 3507 246
SapI GCTCTTCn'nnn_
Cuts at: 0 2604 3753
Size: 2604 1149
ScaI AGT'ACT
Cuts at: 0 1561 3706 3753
Size: 1561 2145 47
Fragments arranged by size:
2145 1561 47
SexAI A'CCwGG_T
Cuts at: 0 3695 3753
Size: 3695 58
SfcI C'TryA_G
Cuts at: 0 450 1281 2240 2501 2559 2842 3753
Size: 450 831 959 261 58 283 911
Fragments arranged by size:
959 911 831 450 283 261 58
SfoI GGC'GCC
Cuts at: 0 1872 3753
Size: 1872 1881
SmlI C'TyrA_G
Cuts at: 0 40 1183 3753
Size: 40 1143 2570
Fragments arranged by size:
2570 1143 40
StyI C'CwwG_G
Cuts at: 0 677 905 1672 2060 2230 2279 2456 3140
Size: 677 228 767 388 170 49 177 684
Cuts at: 3140 3620 3753
Size: 480 133
Fragments arranged by size:
767 684 677 480 388 228 177 170
133 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 548 1402 2693 3753
Size: 548 854 1291 1060
Fragments arranged by size:
1291 1060 854 548
TatI w'GTAC_w
Cuts at: 0 1559 3287 3704 3753
Size: 1559 1728 417 49
Fragments arranged by size:
1728 1559 417 49
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2253 2285 3518 3550 3753
Size: 2253 32 1233 32 203
Fragments arranged by size:
2253 1233 203 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2079 3753
Size: 2079 1674
XhoI C'TCGA_G
Cuts at: 0 40 3753
Size: 40 3713
XmnI GAAnn'nnTTC
Cuts at: 0 2894 3753
Size: 2894 859
ZraI GAC'GTC
Cuts at: 0 1584 3753
Size: 1584 2169
Enzymes that do cut:
AarI AatII AccI AcuI AflII AhdI AlfI AloI
AlwNI ApaI ApaLI ApoI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrFI BstAPI
BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII KasI MmeI
MscI MslI MspA1I NarI NcoI NotI NsiI PasI
PflMI PfoI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuII SacI SacII SalI SapI ScaI SexAI SfcI
SfoI SmlI StyI TaqII TatI TstI Tth111I XhoI
XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflIII AgeI AleI AscI AseI
AsiSI BaeI BmtI BpuEI BsaAI BsaBI BsiWI BsmI
BsmBI BspHI BsrBI BsrGI BssHII BssSI BstBI BstZ17I
CspCI DraI DraIII EcoRV FseI FspI FspAI HindIII
HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI
NruI NspI PacI PciI PmeI PmlI PsiI PsrI
PvuI RsrII SanDI SbfI SfiI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI
XcmI XmaI