Restriction map for
pFN21AE9288
(Linear) (Six-base) MAPSORT of: KIEE9288.seq Check: 872 from: 1 to: 1617
KIEE9288.orf
With 182 enzymes: SgfI *
December 5, 2014 13:29 ..
AatII G_ACGT'C
Cuts at: 0 866 1617
Size: 866 751
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 406 1007 1617
Size: 406 601 610
Fragments arranged by size:
610 601 406
AflIII A'CryG_T
Cuts at: 0 1490 1617
Size: 1490 127
AleI CACnn'nnGTG
Cuts at: 0 691 1102 1249 1617
Size: 691 411 147 368
Fragments arranged by size:
691 411 368 147
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 407 1617
Size: 407 1210
AlwNI CAG_nnn'CTG
Cuts at: 0 132 360 1617
Size: 132 228 1257
Fragments arranged by size:
1257 228 132
ApaI G_GGCC'C
Cuts at: 0 411 731 777 1617
Size: 411 320 46 840
Fragments arranged by size:
840 411 320 46
ApaLI G'TGCA_C
Cuts at: 0 1501 1617
Size: 1501 116
ApoI r'AATT_y
Cuts at: 0 998 1617
Size: 998 619
AvaI C'yCGr_G
Cuts at: 0 36 1617
Size: 36 1581
BanI G'GyrC_C
Cuts at: 0 66 289 576 1617
Size: 66 223 287 1041
Fragments arranged by size:
1041 287 223 66
BanII G_rGCy'C
Cuts at: 0 411 731 777 1573 1617
Size: 411 320 46 796 44
Fragments arranged by size:
796 411 320 46 44
BbeI G_GCGC'C
Cuts at: 0 70 580 1617
Size: 70 510 1037
Fragments arranged by size:
1037 510 70
BbsI GAAGACnn'nnnn_
Cuts at: 0 899 1617
Size: 899 718
BbvCI CC'TCA_GC
Cuts at: 0 749 1617
Size: 749 868
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 27 61 1617
Size: 27 34 1556
Fragments arranged by size:
1556 34 27
BciVI GTATCCnnnnn_n'
Cuts at: 0 495 1617
Size: 495 1122
BclI T'GATC_A
Cuts at: 0 338 1617
Size: 338 1279
BglI GCCn_nnn'nGGC
Cuts at: 0 256 365 1617
Size: 256 109 1252
Fragments arranged by size:
1252 256 109
Bme1580I G_kGCm'C
Cuts at: 0 294 411 731 777 1505 1617
Size: 294 117 320 46 728 112
Fragments arranged by size:
728 320 294 117 112 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 192 348 649 897 1617
Size: 192 156 301 248 720
Fragments arranged by size:
720 301 248 192 156
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 495 1561 1617
Size: 92 403 1066 56
Fragments arranged by size:
1066 403 92 56
Bpu10I CC'TnA_GC
Cuts at: 0 749 1617
Size: 749 868
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 888 1375 1617
Size: 208 680 487 242
Fragments arranged by size:
680 487 242 208
BsaI GGTCTCn'nnnn_
Cuts at: 0 661 1617
Size: 661 956
BsaAI yAC'GTr
Cuts at: 0 1230 1617
Size: 1230 387
BsaHI Gr'CG_yC
Cuts at: 0 67 452 577 863 1617
Size: 67 385 125 286 754
Fragments arranged by size:
754 385 286 125 67
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 153 183 307 337 1617
Size: 153 30 124 30 1280
Fragments arranged by size:
1280 153 124 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 115 328 1072 1168 1617
Size: 115 213 744 96 449
Fragments arranged by size:
744 449 213 115 96
BseYI C'CCAG_C
Cuts at: 0 293 590 682 698 1617
Size: 293 297 92 16 919
Fragments arranged by size:
919 297 293 92 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 1080 1392 1617
Size: 346 734 312 225
Fragments arranged by size:
734 346 312 225
BsiEI CG_ry'CG
Cuts at: 0 1103 1212 1617
Size: 1103 109 405
Fragments arranged by size:
1103 405 109
BsiHKAI G_wGCw'C
Cuts at: 0 113 1148 1505 1617
Size: 113 1035 357 112
Fragments arranged by size:
1035 357 113 112
BsiWI C'GTAC_G
Cuts at: 0 114 1617
Size: 114 1503
BsmBI CGTCTCn'nnnn_
Cuts at: 0 565 854 1617
Size: 565 289 763
Fragments arranged by size:
763 565 289
Bsp1286I G_dGCh'C
Cuts at: 0 113 294 411 731 777 1148 1505 1573
Size: 113 181 117 320 46 371 357 68
Cuts at: 1573 1617
Size: 44
Fragments arranged by size:
371 357 320 181 117 113 68 46
44
BspHI T'CATG_A
Cuts at: 0 1202 1617
Size: 1202 415
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1226 1617
Size: 1226 391
BsrBI CCG'CTC
Cuts at: 0 1167 1617
Size: 1167 450
BsrFI r'CCGG_y
Cuts at: 0 1454 1617
Size: 1454 163
BsrGI T'GTAC_A
Cuts at: 0 1485 1617
Size: 1485 132
BssSI C'ACGA_G
Cuts at: 0 1150 1617
Size: 1150 467
BstAPI GCAn_nnn'nTGC
Cuts at: 0 188 1617
Size: 188 1429
BstYI r'GATC_y
Cuts at: 0 333 1617
Size: 333 1284
BtgI C'CryG_G
Cuts at: 0 21 497 559 580 1496 1617
Size: 21 476 62 21 916 121
Fragments arranged by size:
916 476 121 62 21 21
BtsI GCAGTG_nn'
Cuts at: 0 1354 1617
Size: 1354 263
EaeI y'GGCC_r
Cuts at: 0 395 556 1100 1452 1617
Size: 395 161 544 352 165
Fragments arranged by size:
544 395 352 165 161
EagI C'GGCC_G
Cuts at: 0 1100 1617
Size: 1100 517
EarI CTCTTCn'nnn_
Cuts at: 0 952 1617
Size: 952 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 139 1561 1617
Size: 139 1422 56
Fragments arranged by size:
1422 139 56
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 92 406 495 1007 1561 1617
Size: 92 314 89 512 554 56
Fragments arranged by size:
554 512 314 92 89 56
EcoNI CCTnn'n_nnAGG
Cuts at: 0 853 1617
Size: 853 764
EcoO109I rG'GnC_Cy
Cuts at: 0 156 407 408 650 727 773 774 1015
Size: 156 251 1 242 77 46 1 241
Cuts at: 1015 1530 1617
Size: 515 87
Fragments arranged by size:
515 251 242 241 156 87 77 46
1 1
EcoRI G'AATT_C
Cuts at: 0 998 1617
Size: 998 619
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 543 1617
Size: 543 1074
FspI TGC'GCA
Cuts at: 0 49 1617
Size: 49 1568
HaeII r_GCGC'y
Cuts at: 0 70 132 580 1118 1227 1617
Size: 70 62 448 538 109 390
Fragments arranged by size:
538 448 390 109 70 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 76 108 493 525 1617
Size: 76 32 385 32 1092
Fragments arranged by size:
1092 385 76 32 32
HincII GTy'rAC
Cuts at: 0 246 1617
Size: 246 1371
HindIII A'AGCT_T
Cuts at: 0 1039 1617
Size: 1039 578
KasI G'GCGC_C
Cuts at: 0 66 576 1617
Size: 66 510 1041
Fragments arranged by size:
1041 510 66
MscI TGG'CCA
Cuts at: 0 558 1617
Size: 558 1059
MslI CAynn'nnrTG
Cuts at: 0 389 691 1102 1249 1495 1617
Size: 389 302 411 147 246 122
Fragments arranged by size:
411 389 302 246 147 122
MspA1I CmG'CkG
Cuts at: 0 401 515 1296 1380 1509 1617
Size: 401 114 781 84 129 108
Fragments arranged by size:
781 401 129 114 108 84
NaeI GCC'GGC
Cuts at: 0 1456 1617
Size: 1456 161
NarI GG'CG_CC
Cuts at: 0 67 577 1617
Size: 67 510 1040
Fragments arranged by size:
1040 510 67
NcoI C'CATG_G
Cuts at: 0 497 1496 1617
Size: 497 999 121
Fragments arranged by size:
999 497 121
NgoMIV G'CCGG_C
Cuts at: 0 1454 1617
Size: 1454 163
NspI r_CATG'y
Cuts at: 0 394 1494 1617
Size: 394 1100 123
Fragments arranged by size:
1100 394 123
PasI CC'CwG_GG
Cuts at: 0 610 633 778 1617
Size: 610 23 145 839
Fragments arranged by size:
839 610 145 23
PciI A'CATG_T
Cuts at: 0 1490 1617
Size: 1490 127
PflMI CCAn_nnn'nTGG
Cuts at: 0 1558 1617
Size: 1558 59
PfoI T'CCnGG_A
Cuts at: 0 1160 1617
Size: 1160 457
PmlI CAC'GTG
Cuts at: 0 1230 1617
Size: 1230 387
PpuMI rG'GwC_Cy
Cuts at: 0 156 650 1015 1617
Size: 156 494 365 602
Fragments arranged by size:
602 494 365 156
PspOMI G'GGCC_C
Cuts at: 0 407 727 773 1617
Size: 407 320 46 844
Fragments arranged by size:
844 407 320 46
PstI C_TGCA'G
Cuts at: 0 239 1223 1617
Size: 239 984 394
Fragments arranged by size:
984 394 239
PvuII CAG'CTG
Cuts at: 0 401 515 1617
Size: 401 114 1102
Fragments arranged by size:
1102 401 114
SanDI GG'GwC_CC
Cuts at: 0 650 1015 1617
Size: 650 365 602
Fragments arranged by size:
650 602 365
ScaI AGT'ACT
Cuts at: 0 445 1617
Size: 445 1172
SfcI C'TryA_G
Cuts at: 0 235 1219 1617
Size: 235 984 398
Fragments arranged by size:
984 398 235
SfoI GGC'GCC
Cuts at: 0 68 578 1617
Size: 68 510 1039
Fragments arranged by size:
1039 510 68
SmlI C'TyrA_G
Cuts at: 0 36 223 867 1390 1617
Size: 36 187 644 523 227
Fragments arranged by size:
644 523 227 187 36
StuI AGG'CCT
Cuts at: 0 523 1617
Size: 523 1094
StyI C'CwwG_G
Cuts at: 0 497 1496 1617
Size: 497 999 121
Fragments arranged by size:
999 497 121
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 369 778 1617
Size: 369 409 839
Fragments arranged by size:
839 409 369
TatI w'GTAC_w
Cuts at: 0 443 1485 1617
Size: 443 1042 132
Fragments arranged by size:
1042 443 132
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 153 185 261 293 307 339 1617
Size: 153 32 76 32 14 32 1278
Fragments arranged by size:
1278 153 76 32 32 32 14
Tth111I GACn'n_nGTC
Cuts at: 0 432 1617
Size: 432 1185
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 133 690 1617
Size: 133 557 927
Fragments arranged by size:
927 557 133
XhoI C'TCGA_G
Cuts at: 0 36 1617
Size: 36 1581
ZraI GAC'GTC
Cuts at: 0 864 1617
Size: 864 753
Enzymes that do cut:
AatII AcuI AflIII AleI AlfI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BclI BglI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI
BsrGI BssSI BstAPI BstYI BtgI BtsI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI
PfoI PmlI PpuMI PspOMI PstI PvuII SanDI ScaI
SfcI SfoI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI AscI AseI AsiSI AvrII BaeI BamHI BfrBI
BglII BlpI BmgBI BmtI BplI BsaBI BsaWI BsmI
BspEI BsrDI BssHII BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EcoICRI EcoRV
FseI FspAI HpaI KpnI MfeI MluI MmeI NdeI
NheI NotI NruI NsiI PacI PmeI PpiI PshAI
PsiI PspXI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XmaI XmnI