Restriction map for
pFN21AE9282
(Linear) (Six-base) MAPSORT of: KIEE9282.seq Check: 4615 from: 1 to: 3747
KIEE9282.orf
With 182 enzymes: SgfI *
December 5, 2014 13:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2208 3747
Size: 2208 1539
AccI GT'mk_AC
Cuts at: 0 2719 3747
Size: 2719 1028
Acc65I G'GTAC_C
Cuts at: 0 1945 3747
Size: 1945 1802
AclI AA'CG_TT
Cuts at: 0 1734 2118 3747
Size: 1734 384 1629
Fragments arranged by size:
1734 1629 384
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 3305 3437 3747
Size: 260 3045 132 310
Fragments arranged by size:
3045 310 260 132
AhdI GACnn_n'nnGTC
Cuts at: 0 2715 3747
Size: 2715 1032
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 916 3747
Size: 916 2831
ApaLI G'TGCA_C
Cuts at: 0 2627 3747
Size: 2627 1120
ApoI r'AATT_y
Cuts at: 0 87 479 1926 2069 2199 2348 2453 2647
Size: 87 392 1447 143 130 149 105 194
Cuts at: 2647 2796 2862 3590 3747
Size: 149 66 728 157
Fragments arranged by size:
1447 728 392 194 157 149 149 143
130 105 87 66
AseI AT'TA_AT
Cuts at: 0 1337 1808 3747
Size: 1337 471 1939
Fragments arranged by size:
1939 1337 471
AvaI C'yCGr_G
Cuts at: 0 1064 1205 1253 3067 3747
Size: 1064 141 48 1814 680
Fragments arranged by size:
1814 1064 680 141 48
BamHI G'GATC_C
Cuts at: 0 3297 3747
Size: 3297 450
BanI G'GyrC_C
Cuts at: 0 1945 3747
Size: 1945 1802
BanII G_rGCy'C
Cuts at: 0 788 2265 2523 3068 3747
Size: 788 1477 258 545 679
Fragments arranged by size:
1477 788 679 545 258
BbsI GAAGACnn'nnnn_
Cuts at: 0 2167 2419 3747
Size: 2167 252 1328
Fragments arranged by size:
2167 1328 252
BbvCI CC'TCA_GC
Cuts at: 0 3560 3747
Size: 3560 187
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1183 1217 1304 1338 3747
Size: 1183 34 87 34 2409
Fragments arranged by size:
2409 1183 87 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2691 3747
Size: 2691 1056
BclI T'GATC_A
Cuts at: 0 960 3747
Size: 960 2787
BglII A'GATC_T
Cuts at: 0 2370 3747
Size: 2370 1377
BlpI GC'TnA_GC
Cuts at: 0 159 3747
Size: 159 3588
Bme1580I G_kGCm'C
Cuts at: 0 2631 3747
Size: 2631 1116
BmgBI CAC'GTC
Cuts at: 0 316 3747
Size: 316 3431
BmrI ACTGGGnnnn_n'
Cuts at: 0 3676 3747
Size: 3676 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1924 2170 2653 2874 3747
Size: 1924 246 483 221 873
Fragments arranged by size:
1924 873 483 246 221
Bpu10I CC'TnA_GC
Cuts at: 0 3062 3560 3747
Size: 3062 498 187
Fragments arranged by size:
3062 498 187
BsaBI GATnn'nnATC
Cuts at: 0 1148 3747
Size: 1148 2599
BsaWI w'CCGG_w
Cuts at: 0 788 3747
Size: 788 2959
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1546 2201 3747
Size: 1546 655 1546
Fragments arranged by size:
1546 1546 655
BsiHKAI G_wGCw'C
Cuts at: 0 788 1206 2034 2265 2523 2631 3068 3747
Size: 788 418 828 231 258 108 437 679
Fragments arranged by size:
828 788 679 437 418 258 231 108
Bsp1286I G_dGCh'C
Cuts at: 0 788 1206 2034 2265 2523 2631 3068 3747
Size: 788 418 828 231 258 108 437 679
Fragments arranged by size:
828 788 679 437 418 258 231 108
BspEI T'CCGG_A
Cuts at: 0 788 3747
Size: 788 2959
BspHI T'CATG_A
Cuts at: 0 1302 3678 3747
Size: 1302 2376 69
Fragments arranged by size:
2376 1302 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2208 3747
Size: 2208 1539
BsrBI CCG'CTC
Cuts at: 0 3276 3747
Size: 3276 471
BsrDI GCAATG_nn'
Cuts at: 0 519 3747
Size: 519 3228
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2485 3747
Size: 2485 1262
BstYI r'GATC_y
Cuts at: 0 2370 2959 3297 3747
Size: 2370 589 338 450
Fragments arranged by size:
2370 589 450 338
Bsu36I CC'TnA_GG
Cuts at: 0 857 3747
Size: 857 2890
BtsI GCAGTG_nn'
Cuts at: 0 1448 3747
Size: 1448 2299
DraI TTT'AAA
Cuts at: 0 1148 2741 3702 3747
Size: 1148 1593 961 45
Fragments arranged by size:
1593 1148 961 45
EaeI y'GGCC_r
Cuts at: 0 633 2436 3747
Size: 633 1803 1311
Fragments arranged by size:
1803 1311 633
EcoICRI GAG'CTC
Cuts at: 0 786 2263 2521 3066 3747
Size: 786 1477 258 545 681
Fragments arranged by size:
1477 786 681 545 258
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 1924 2170 2653 2874 3305 3437 3747
Size: 260 1664 246 483 221 431 132 310
Fragments arranged by size:
1664 483 431 310 260 246 221 132
EcoO109I rG'GnC_Cy
Cuts at: 0 1960 2468 2481 3747
Size: 1960 508 13 1266
Fragments arranged by size:
1960 1266 508 13
EcoRI G'AATT_C
Cuts at: 0 87 479 2348 3590 3747
Size: 87 392 1869 1242 157
Fragments arranged by size:
1869 1242 392 157 87
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 63 1258 2990 3747
Size: 63 1195 1732 757
Fragments arranged by size:
1732 1195 757 63
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1369 1401 2286 2318 2701 2733 3055 3087
Size: 1369 32 885 32 383 32 322 32
Cuts at: 3087 3325 3357 3747
Size: 238 32 390
Fragments arranged by size:
1369 885 390 383 322 238 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 1278 3747
Size: 1278 2469
HindIII A'AGCT_T
Cuts at: 0 59 156 3009 3747
Size: 59 97 2853 738
Fragments arranged by size:
2853 738 97 59
KpnI G_GTAC'C
Cuts at: 0 1949 3747
Size: 1949 1798
MfeI C'AATT_G
Cuts at: 0 26 247 3747
Size: 26 221 3500
Fragments arranged by size:
3500 221 26
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1951 3747
Size: 110 1841 1796
Fragments arranged by size:
1841 1796 110
MscI TGG'CCA
Cuts at: 0 635 3747
Size: 635 3112
MslI CAynn'nnrTG
Cuts at: 0 845 2507 3747
Size: 845 1662 1240
Fragments arranged by size:
1662 1240 845
MspA1I CmG'CkG
Cuts at: 0 1789 1902 3747
Size: 1789 113 1845
Fragments arranged by size:
1845 1789 113
NspI r_CATG'y
Cuts at: 0 269 3732 3747
Size: 269 3463 15
Fragments arranged by size:
3463 269 15
PflMI CCAn_nnn'nTGG
Cuts at: 0 1522 3747
Size: 1522 2225
PfoI T'CCnGG_A
Cuts at: 0 1669 2828 2918 3747
Size: 1669 1159 90 829
Fragments arranged by size:
1669 1159 829 90
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2108 2140 3260 3292 3747
Size: 2108 32 1120 32 455
Fragments arranged by size:
2108 1120 455 32 32
PsiI TTA'TAA
Cuts at: 0 56 1643 3747
Size: 56 1587 2104
Fragments arranged by size:
2104 1587 56
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1220 1252 3747
Size: 1220 32 2495
Fragments arranged by size:
2495 1220 32
PstI C_TGCA'G
Cuts at: 0 1755 2219 3747
Size: 1755 464 1528
Fragments arranged by size:
1755 1528 464
PvuII CAG'CTG
Cuts at: 0 1789 1902 3747
Size: 1789 113 1845
Fragments arranged by size:
1845 1789 113
SacI G_AGCT'C
Cuts at: 0 788 2265 2523 3068 3747
Size: 788 1477 258 545 679
Fragments arranged by size:
1477 788 679 545 258
SfcI C'TryA_G
Cuts at: 0 1326 1751 2215 2374 2836 3747
Size: 1326 425 464 159 462 911
Fragments arranged by size:
1326 911 464 462 425 159
SphI G_CATG'C
Cuts at: 0 269 3747
Size: 269 3478
StuI AGG'CCT
Cuts at: 0 1045 3747
Size: 1045 2702
StyI C'CwwG_G
Cuts at: 0 303 984 1964 2064 2484 2639 3747
Size: 303 681 980 100 420 155 1108
Fragments arranged by size:
1108 980 681 420 303 155 100
SwaI ATTT'AAAT
Cuts at: 0 1148 3747
Size: 1148 2599
TatI w'GTAC_w
Cuts at: 0 243 537 2222 2744 3747
Size: 243 294 1685 522 1003
Fragments arranged by size:
1685 1003 522 294 243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1965 1997 2824 2856 3747
Size: 1965 32 827 32 891
Fragments arranged by size:
1965 891 827 32 32
XbaI T'CTAG_A
Cuts at: 0 1116 3747
Size: 1116 2631
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AhdI AlfI ApaLI
ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaBI BsaWI BseRI BsiHKAI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BstXI BstYI Bsu36I BtsI
DraI EaeI EcoICRI Eco57MI EcoO109I EcoRI FalI Hin4I
HincII HindIII KpnI MfeI MmeI MscI MslI MspA1I
NspI PflMI PfoI PpiI PsiI PsrI PstI PvuII
SacI SfcI SphI StuI StyI SwaI TatI TstI
XbaI
Enzymes that do not cut:
AatII AfeI AflII AflIII AgeI AleI AloI AlwNI
ApaI AscI AsiSI AvrII BaeI BbeI BfrBI BglI
BmtI BplI BpuEI BsaI BsaAI BsaHI BsaXI BseYI
BsgI BsiEI BsiWI BsmI BsmBI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstZ17I BtgI BtgZI ClaI
CspCI DraIII DrdI EagI EarI EciI EcoNI EcoRV
FseI FspI FspAI HaeII HpaI KasI MluI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpuMI PshAI PspOMI
PspXI PvuI RsrII SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SspI TaqII Tth111I XcmI XhoI
XmaI XmnI ZraI