(Linear) (Six-base) MAPSORT of: KIEE9281.seq  Check: 5145  from: 1  to: 627



KIEE9281.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     192     627 
   Size:        192     435
 
AccI GT'mk_AC

Cuts at:      0     373     627 
   Size:        373     254
 
AlwNI CAG_nnn'CTG

Cuts at:      0     553     627 
   Size:        553      74
 
ApaI G_GGCC'C

Cuts at:      0     513     627 
   Size:        513     114
 
ApoI r'AATT_y

Cuts at:      0     201     218     627 
   Size:        201      17     409

  Fragments arranged by size: 

                 409     201      17
 
AvaI C'yCGr_G

Cuts at:      0      73     627 
   Size:         73     554
 
BanII G_rGCy'C

Cuts at:      0      74     513     627 
   Size:         74     439     114

  Fragments arranged by size: 

                 439     114      74
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     379     413     627 
   Size:        379      34     214

  Fragments arranged by size: 

                 379     214      34
 
Bme1580I G_kGCm'C

Cuts at:      0     513     627 
   Size:        513     114
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      97     627 
   Size:         97     530
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     542     627 
   Size:        104     438      85

  Fragments arranged by size: 

                 438     104      85
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     350     627 
   Size:        350     277
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     366     627 
   Size:        366     261
 
BsiHKAI G_wGCw'C

Cuts at:      0      74     627 
   Size:         74     553
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     292     336     627 
   Size:        292      44     291

  Fragments arranged by size: 

                 292     291      44
 
Bsp1286I G_dGCh'C

Cuts at:      0      74     513     627 
   Size:         74     439     114

  Fragments arranged by size: 

                 439     114      74
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     192     627 
   Size:        192     435
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     480     627 
   Size:        480     147
 
BstYI r'GATC_y

Cuts at:      0     263     627 
   Size:        263     364
 
BstZ17I GTA'TAC

Cuts at:      0     374     627 
   Size:        374     253
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     369     627 
   Size:        369     258
 
EarI CTCTTCn'nnn_

Cuts at:      0     419     627 
   Size:        419     208
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      34     627 
   Size:         34     593
 
EcoICRI GAG'CTC

Cuts at:      0      72     627 
   Size:         72     555
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     542     627 
   Size:        104     438      85

  Fragments arranged by size: 

                 438     104      85
 
EcoO109I rG'GnC_Cy

Cuts at:      0      78     509     510     627 
   Size:         78     431       1     117

  Fragments arranged by size: 

                 431     117      78       1
 
EcoRV GAT'ATC

Cuts at:      0     163     627 
   Size:        163     464
 
HaeII r_GCGC'y

Cuts at:      0      29     627 
   Size:         29     598
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     224     256     627 
   Size:        224      32     371

  Fragments arranged by size: 

                 371     224      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     390     627 
   Size:        390     237
 
MslI CAynn'nnrTG

Cuts at:      0     478     627 
   Size:        478     149
 
PasI CC'CwG_GG

Cuts at:      0     259     627 
   Size:        259     368
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     109     627 
   Size:        109     518
 
PspOMI G'GGCC_C

Cuts at:      0     509     627 
   Size:        509     118
 
PspXI vC'TCGA_Gb

Cuts at:      0      73     627 
   Size:         73     554
 
SacI G_AGCT'C

Cuts at:      0      74     627 
   Size:         74     553
 
SfcI C'TryA_G

Cuts at:      0     419     627 
   Size:        419     208
 
SmlI C'TyrA_G

Cuts at:      0      73     365     627 
   Size:         73     292     262

  Fragments arranged by size: 

                 292     262      73
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     477     627 
   Size:        477     150
 
XhoI C'TCGA_G

Cuts at:      0      73     627 
   Size:         73     554
 

 Enzymes that do cut:

     AarI     AccI    AlwNI     ApaI     ApoI     AvaI    BanII     BcgI
 Bme1580I     BmrI     BpmI    BpuEI     BsaI  BsiHKAI    BsmBI Bsp1286I
    BspMI    BstXI    BstYI  BstZ17I    BtgZI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRV    HaeII    Hin4I     MmeI     MslI     PasI
    PflMI   PspOMI    PspXI     SacI     SfcI     SmlI     XcmI     XhoI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI
    AvrII     BaeI    BamHI     BanI     BbeI     BbsI    BbvCI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmtI     BplI
   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII   Bsu36I
     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI    EcoNI    EcoRI     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XmaI     XmnI     ZraI