Restriction map for
pFN21AE9280
(Linear) (Six-base) MAPSORT of: KIEE9280.seq Check: 1788 from: 1 to: 1128
KIEE9280.orf
With 182 enzymes: SgfI *
December 5, 2014 13:29 ..
AatII G_ACGT'C
Cuts at: 0 868 1128
Size: 868 260
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 894 1128
Size: 894 234
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 547 579 983 1015 1128
Size: 547 32 404 32 113
Fragments arranged by size:
547 404 113 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 308 444 462 948 1128
Size: 308 136 18 486 180
Fragments arranged by size:
486 308 180 136 18
ApoI r'AATT_y
Cuts at: 0 382 1128
Size: 382 746
AvaI C'yCGr_G
Cuts at: 0 33 667 1128
Size: 33 634 461
Fragments arranged by size:
634 461 33
BamHI G'GATC_C
Cuts at: 0 431 1128
Size: 431 697
BanI G'GyrC_C
Cuts at: 0 1114 1128
Size: 1114 14
BanII G_rGCy'C
Cuts at: 0 244 512 668 1128
Size: 244 268 156 460
Fragments arranged by size:
460 268 244 156
BbvCI CC'TCA_GC
Cuts at: 0 824 1128
Size: 824 304
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 736 770 1128
Size: 736 34 358
Fragments arranged by size:
736 358 34
BglI GCCn_nnn'nGGC
Cuts at: 0 597 1128
Size: 597 531
Bme1580I G_kGCm'C
Cuts at: 0 147 1119 1128
Size: 147 972 9
Fragments arranged by size:
972 147 9
BmrI ACTGGGnnnn_n'
Cuts at: 0 786 989 1128
Size: 786 203 139
Fragments arranged by size:
786 203 139
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 894 1128
Size: 894 234
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 466 682 925 977 1000 1128
Size: 151 315 216 243 52 23 128
Fragments arranged by size:
315 243 216 151 128 52 23
Bpu10I CC'TnA_GC
Cuts at: 0 824 1128
Size: 824 304
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1128
Size: 97 1031
BsaI GGTCTCn'nnnn_
Cuts at: 0 775 1128
Size: 775 353
BsaHI Gr'CG_yC
Cuts at: 0 865 1128
Size: 865 263
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 103 190 193 364 404 1128
Size: 103 87 3 171 40 724
Fragments arranged by size:
724 171 103 87 40 3
BseYI C'CCAG_C
Cuts at: 0 896 1128
Size: 896 232
BsiEI CG_ry'CG
Cuts at: 0 25 1128
Size: 25 1103
Bsp1286I G_dGCh'C
Cuts at: 0 147 244 512 668 1119 1128
Size: 147 97 268 156 451 9
Fragments arranged by size:
451 268 156 147 97 9
BsrBI CCG'CTC
Cuts at: 0 135 961 1128
Size: 135 826 167
Fragments arranged by size:
826 167 135
BsrFI r'CCGG_y
Cuts at: 0 289 1128
Size: 289 839
BstXI CCAn_nnnn'nTGG
Cuts at: 0 372 1128
Size: 372 756
BstYI r'GATC_y
Cuts at: 0 108 431 492 1128
Size: 108 323 61 636
Fragments arranged by size:
636 323 108 61
Bsu36I CC'TnA_GG
Cuts at: 0 463 1128
Size: 463 665
BtgI C'CryG_G
Cuts at: 0 204 606 726 1128
Size: 204 402 120 402
Fragments arranged by size:
402 402 204 120
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 728 1128
Size: 728 400
DrdI GACnn_nn'nnGTC
Cuts at: 0 1015 1128
Size: 1015 113
EaeI y'GGCC_r
Cuts at: 0 22 45 287 598 1032 1082 1128
Size: 22 23 242 311 434 50 46
Fragments arranged by size:
434 311 242 50 46 23 22
EagI C'GGCC_G
Cuts at: 0 22 1128
Size: 22 1106
EarI CTCTTCn'nnn_
Cuts at: 0 167 1128
Size: 167 961
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 1128
Size: 19 1109
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 466 682 894 925 977 1000 1128
Size: 151 315 216 212 31 52 23 128
Fragments arranged by size:
315 216 212 151 128 52 31 23
EcoNI CCTnn'n_nnAGG
Cuts at: 0 172 466 1105 1128
Size: 172 294 639 23
Fragments arranged by size:
639 294 172 23
EcoO109I rG'GnC_Cy
Cuts at: 0 750 828 936 1128
Size: 750 78 108 192
Fragments arranged by size:
750 192 108 78
EcoRI G'AATT_C
Cuts at: 0 382 1128
Size: 382 746
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 492 1128
Size: 492 636
HaeII r_GCGC'y
Cuts at: 0 168 1128
Size: 168 960
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 541 560 573 592 1128
Size: 541 19 13 19 536
Fragments arranged by size:
541 536 19 19 13
HincII GTy'rAC
Cuts at: 0 621 1128
Size: 621 507
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 816 1128
Size: 816 312
MscI TGG'CCA
Cuts at: 0 1034 1128
Size: 1034 94
MslI CAynn'nnrTG
Cuts at: 0 580 1128
Size: 580 548
MspA1I CmG'CkG
Cuts at: 0 141 187 206 423 1128
Size: 141 46 19 217 705
Fragments arranged by size:
705 217 141 46 19
NcoI C'CATG_G
Cuts at: 0 726 1128
Size: 726 402
NspI r_CATG'y
Cuts at: 0 543 919 1128
Size: 543 376 209
Fragments arranged by size:
543 376 209
PflMI CCAn_nnn'nTGG
Cuts at: 0 948 1128
Size: 948 180
PfoI T'CCnGG_A
Cuts at: 0 248 1128
Size: 248 880
PpuMI rG'GwC_Cy
Cuts at: 0 750 1128
Size: 750 378
PstI C_TGCA'G
Cuts at: 0 440 1094 1128
Size: 440 654 34
Fragments arranged by size:
654 440 34
PvuII CAG'CTG
Cuts at: 0 423 1128
Size: 423 705
SacII CC_GC'GG
Cuts at: 0 207 1128
Size: 207 921
SfcI C'TryA_G
Cuts at: 0 436 496 1090 1128
Size: 436 60 594 38
Fragments arranged by size:
594 436 60 38
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 597 1128
Size: 597 531
SmaI CCC'GGG
Cuts at: 0 35 669 1128
Size: 35 634 459
Fragments arranged by size:
634 459 35
SmlI C'TyrA_G
Cuts at: 0 112 1128
Size: 112 1016
SphI G_CATG'C
Cuts at: 0 919 1128
Size: 919 209
SrfI GCCC'GGGC
Cuts at: 0 669 1128
Size: 669 459
StyI C'CwwG_G
Cuts at: 0 726 1128
Size: 726 402
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 722 1128
Size: 722 406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 321 981 1128
Size: 321 660 147
Fragments arranged by size:
660 321 147
XmaI C'CCGG_G
Cuts at: 0 33 667 1128
Size: 33 634 461
Fragments arranged by size:
634 461 33
XmnI GAAnn'nnTTC
Cuts at: 0 472 1128
Size: 472 656
ZraI GAC'GTC
Cuts at: 0 866 1128
Size: 866 262
Enzymes that do cut:
AatII AcuI AloI AlwNI ApoI AvaI BamHI BanI
BanII BbvCI BcgI BglI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaHI BseRI BseYI BsiEI Bsp1286I
BsrBI BsrFI BstXI BstYI Bsu36I BtgI BtgZI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII MmeI MscI MslI MspA1I
NcoI NspI PflMI PfoI PpuMI PstI PvuII SacII
SfcI SfiI SmaI SmlI SphI SrfI StyI TaqII
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbsI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BsaAI BsaBI BsaWI BsaXI BsgI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtsI
ClaI CspCI DraI DraIII EcoICRI EcoRV FseI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SalI SanDI SapI
SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI
SspI StuI SwaI TatI TstI Tth111I XbaI XhoI