(Linear) (Six-base) MAPSORT of: KIEE9278.seq  Check: 3542  from: 1  to: 189



KIEE9278.orf

 With 182 enzymes: SgfI * 

                        December 5, 2014 13:29  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      68     189 
   Size:         68     121
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      70     189 
   Size:         70     119
 
AlwNI CAG_nnn'CTG

Cuts at:      0      16     189 
   Size:         16     173
 
BamHI G'GATC_C

Cuts at:      0      19     189 
   Size:         19     170
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     131     189 
   Size:        131      58
 
BmgBI CAC'GTC

Cuts at:      0     144     189 
   Size:        144      45
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     189 
   Size:        175      14
 
BsaWI w'CCGG_w

Cuts at:      0      29     189 
   Size:         29     160
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40      46     189 
   Size:         40       6     143

  Fragments arranged by size: 

                 143      40       6
 
BspEI T'CCGG_A

Cuts at:      0      29     189 
   Size:         29     160
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      68     189 
   Size:         68     121
 
BstYI r'GATC_y

Cuts at:      0      19     189 
   Size:         19     170
 
BtgI C'CryG_G

Cuts at:      0      99     189 
   Size:         99      90
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175     189 
   Size:        175      14
 
MspA1I CmG'CkG

Cuts at:      0     153     174     189 
   Size:        153      21      15

  Fragments arranged by size: 

                 153      21      15
 
PfoI T'CCnGG_A

Cuts at:      0     179     189 
   Size:        179      10
 
PvuII CAG'CTG

Cuts at:      0     153     174     189 
   Size:        153      21      15

  Fragments arranged by size: 

                 153      21      15
 

 Enzymes that do cut:

     AarI     AlfI    AlwNI    BamHI     BbsI    BmgBI     BpmI    BsaWI
     BsgI    BspEI    BspMI    BstYI     BtgI  Eco57MI   MspA1I     PfoI
    PvuII

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI