Restriction map for
pFN21AE9259
(Linear) (Six-base) MAPSORT of: KIEE9259.seq Check: 5966 from: 1 to: 3630
KIEE9259.orf
With 182 enzymes: SgfI *
April 20, 2012 13:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2266 2918 3508 3630
Size: 2266 652 590 122
Fragments arranged by size:
2266 652 590 122
AatII G_ACGT'C
Cuts at: 0 2333 2432 2878 3630
Size: 2333 99 446 752
Fragments arranged by size:
2333 752 446 99
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 792 887 1643 3399 3630
Size: 792 95 756 1756 231
Fragments arranged by size:
1756 792 756 231 95
AfeI AGC'GCT
Cuts at: 0 2680 3630
Size: 2680 950
AflIII A'CryG_T
Cuts at: 0 2687 2923 3630
Size: 2687 236 707
Fragments arranged by size:
2687 707 236
AhdI GACnn_n'nnGTC
Cuts at: 0 768 861 3630
Size: 768 93 2769
Fragments arranged by size:
2769 768 93
AleI CACnn'nnGTG
Cuts at: 0 2926 3341 3630
Size: 2926 415 289
Fragments arranged by size:
2926 415 289
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2080 3127 3630
Size: 2080 1047 503
Fragments arranged by size:
2080 1047 503
AlwNI CAG_nnn'CTG
Cuts at: 0 205 493 1687 3630
Size: 205 288 1194 1943
Fragments arranged by size:
1943 1194 288 205
ApaI G_GGCC'C
Cuts at: 0 1823 3630
Size: 1823 1807
ApoI r'AATT_y
Cuts at: 0 3013 3630
Size: 3013 617
AvaI C'yCGr_G
Cuts at: 0 766 1482 1646 1717 2576 2674 2780 3422
Size: 766 716 164 71 859 98 106 642
Cuts at: 3422 3598 3630
Size: 176 32
Fragments arranged by size:
859 766 716 642 176 164 106 98
71 32
BamHI G'GATC_C
Cuts at: 0 317 3630
Size: 317 3313
BanI G'GyrC_C
Cuts at: 0 1293 1510 1775 2422 3630
Size: 1293 217 265 647 1208
Fragments arranged by size:
1293 1208 647 265 217
BanII G_rGCy'C
Cuts at: 0 471 520 549 1132 1823 2089 2834 3605
Size: 471 49 29 583 691 266 745 771
Cuts at: 3605 3630
Size: 25
Fragments arranged by size:
771 745 691 583 471 266 49 29
25
BbeI G_GCGC'C
Cuts at: 0 1779 2426 3630
Size: 1779 647 1204
Fragments arranged by size:
1779 1204 647
BbsI GAAGACnn'nnnn_
Cuts at: 0 1584 3363 3630
Size: 1584 1779 267
Fragments arranged by size:
1779 1584 267
BbvCI CC'TCA_GC
Cuts at: 0 3224 3630
Size: 3224 406
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3526 3560 3630
Size: 3526 34 70
Fragments arranged by size:
3526 70 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1875 2960 3509 3630
Size: 1875 1085 549 121
Fragments arranged by size:
1875 1085 549 121
BglII A'GATC_T
Cuts at: 0 666 2096 3630
Size: 666 1430 1534
Fragments arranged by size:
1534 1430 666
BlpI GC'TnA_GC
Cuts at: 0 2311 2968 3630
Size: 2311 657 662
Fragments arranged by size:
2311 662 657
Bme1580I G_kGCm'C
Cuts at: 0 1331 1513 1749 1823 2801 3630
Size: 1331 182 236 74 978 829
Fragments arranged by size:
1331 978 829 236 182 74
BmgBI CAC'GTC
Cuts at: 0 2688 2775 3630
Size: 2688 87 855
Fragments arranged by size:
2688 855 87
BmrI ACTGGGnnnn_n'
Cuts at: 0 658 1136 1736 1882 2478 3446 3630
Size: 658 478 600 146 596 968 184
Fragments arranged by size:
968 658 600 596 478 184 146
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 3231 3624 3630
Size: 3231 393 6
Fragments arranged by size:
3231 393 6
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 182 196 488 796 1195 1261 1540
Size: 97 85 14 292 308 399 66 279
Cuts at: 1540 2141 2212 2992 3090 3571 3630
Size: 601 71 780 98 481 59
Fragments arranged by size:
780 601 481 399 308 292 279 98
97 85 71 66 59 14
Bpu10I CC'TnA_GC
Cuts at: 0 1815 2630 3224 3587 3630
Size: 1815 815 594 363 43
Fragments arranged by size:
1815 815 594 363 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 1375 1613 3630
Size: 307 1068 238 2017
Fragments arranged by size:
2017 1068 307 238
BsaI GGTCTCn'nnnn_
Cuts at: 0 1393 1438 3630
Size: 1393 45 2192
Fragments arranged by size:
2192 1393 45
BsaAI yAC'GTr
Cuts at: 0 2617 2924 3630
Size: 2617 307 706
Fragments arranged by size:
2617 706 307
BsaHI Gr'CG_yC
Cuts at: 0 1776 2158 2330 2359 2423 2429 2875 3630
Size: 1776 382 172 29 64 6 446 755
Fragments arranged by size:
1776 755 446 382 172 64 29 6
BsaWI w'CCGG_w
Cuts at: 0 2975 3431 3630
Size: 2975 456 199
Fragments arranged by size:
2975 456 199
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1668 1698 2458 2488 3436 3466 3630
Size: 1668 30 760 30 948 30 164
Fragments arranged by size:
1668 948 760 164 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 107 727 793 916 919 922 931 955
Size: 107 620 66 123 3 3 9 24
Cuts at: 955 958 988 1122 1127 1276 2257 2464 3457
Size: 3 30 134 5 149 981 207 993
Cuts at: 3457 3630
Size: 173
Fragments arranged by size:
993 981 620 207 173 149 134 123
107 66 30 24 9 5 3 3
3
BseYI C'CCAG_C
Cuts at: 0 324 358 1183 1213 3146 3630
Size: 324 34 825 30 1933 484
Fragments arranged by size:
1933 825 484 324 34 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1296 1485 1673 2233 2449 2548 2904 3630
Size: 1296 189 188 560 216 99 356 726
Fragments arranged by size:
1296 726 560 356 216 189 188 99
BsiEI CG_ry'CG
Cuts at: 0 35 1673 2895 3468 3630
Size: 35 1638 1222 573 162
Fragments arranged by size:
1638 1222 573 162 35
BsiHKAI G_wGCw'C
Cuts at: 0 1439 2317 2384 3064 3605 3630
Size: 1439 878 67 680 541 25
Fragments arranged by size:
1439 878 680 541 67 25
BsmI GAATG_Cn'
Cuts at: 0 2520 3630
Size: 2520 1110
BsmBI CGTCTCn'nnnn_
Cuts at: 0 781 1070 2866 3630
Size: 781 289 1796 764
Fragments arranged by size:
1796 781 764 289
Bsp1286I G_dGCh'C
Cuts at: 0 471 520 549 1132 1331 1439 1513 1749
Size: 471 49 29 583 199 108 74 236
Cuts at: 1749 1823 2089 2317 2384 2801 2834 3064 3605
Size: 74 266 228 67 417 33 230 541
Cuts at: 3605 3630
Size: 25
Fragments arranged by size:
583 541 471 417 266 236 230 228
199 108 74 74 67 49 33 29
25
BspEI T'CCGG_A
Cuts at: 0 3431 3630
Size: 3431 199
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2266 2918 3398 3508 3630
Size: 2266 652 480 110 122
Fragments arranged by size:
2266 652 480 122 110
BsrDI GCAATG_nn'
Cuts at: 0 1383 3542 3630
Size: 1383 2159 88
Fragments arranged by size:
2159 1383 88
BsrFI r'CCGG_y
Cuts at: 0 1013 1668 1672 3630
Size: 1013 655 4 1958
Fragments arranged by size:
1958 1013 655 4
BssHII G'CGCG_C
Cuts at: 0 2518 3630
Size: 2518 1112
BssSI C'ACGA_G
Cuts at: 0 513 1109 3630
Size: 513 596 2521
Fragments arranged by size:
2521 596 513
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1687 2978 3630
Size: 1687 1291 652
Fragments arranged by size:
1687 1291 652
BstEII G'GTnAC_C
Cuts at: 0 657 1635 1659 3630
Size: 657 978 24 1971
Fragments arranged by size:
1971 978 657 24
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1422 2347 3549 3630
Size: 1422 925 1202 81
Fragments arranged by size:
1422 1202 925 81
BstYI r'GATC_y
Cuts at: 0 317 666 2096 3630
Size: 317 349 1430 1534
Fragments arranged by size:
1534 1430 349 317
Bsu36I CC'TnA_GG
Cuts at: 0 1134 2840 3630
Size: 1134 1706 790
Fragments arranged by size:
1706 1134 790
BtgI C'CryG_G
Cuts at: 0 306 1051 1093 1859 2090 2174 2547 3630
Size: 306 745 42 766 231 84 373 1083
Fragments arranged by size:
1083 766 745 373 306 231 84 42
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2481 2553 3390 3630
Size: 2481 72 837 240
Fragments arranged by size:
2481 837 240 72
BtsI GCAGTG_nn'
Cuts at: 0 1473 1676 2144 2916 3630
Size: 1473 203 468 772 714
Fragments arranged by size:
1473 772 714 468 203
ClaI AT'CG_AT
Cuts at: 0 2891 3630
Size: 2891 739
EaeI y'GGCC_r
Cuts at: 0 32 671 1011 1340 1670 2961 3630
Size: 32 639 340 329 330 1291 669
Fragments arranged by size:
1291 669 639 340 330 329 32
EagI C'GGCC_G
Cuts at: 0 32 1670 3630
Size: 32 1638 1960
Fragments arranged by size:
1960 1638 32
EarI CTCTTCn'nnn_
Cuts at: 0 357 893 908 926 932 944 956 3262
Size: 357 536 15 18 6 12 12 2306
Cuts at: 3262 3630
Size: 368
Fragments arranged by size:
2306 536 368 357 18 15 12 12
6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1944 3630
Size: 1944 1686
EcoICRI GAG'CTC
Cuts at: 0 3603 3630
Size: 3603 27
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 182 196 488 792 796 887 1195
Size: 97 85 14 292 304 4 91 308
Cuts at: 1195 1261 1540 1643 2141 2212 2992 3090 3399
Size: 66 279 103 498 71 780 98 309
Cuts at: 3399 3571 3630
Size: 172 59
Fragments arranged by size:
780 498 309 308 304 292 279 172
103 98 97 91 85 71 66 59
14 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 154 709 1705 1897 3630
Size: 154 555 996 192 1733
Fragments arranged by size:
1733 996 555 192 154
EcoO109I rG'GnC_Cy
Cuts at: 0 48 300 762 770 1138 1158 1169 1193
Size: 48 252 462 8 368 20 11 24
Cuts at: 1193 1819 1820 1890 1902 2664 3182 3630
Size: 626 1 70 12 762 518 448
Fragments arranged by size:
762 626 518 462 448 368 252 70
48 24 20 12 11 8 1
HaeII r_GCGC'y
Cuts at: 0 70 1779 2426 2492 2682 3630
Size: 70 1709 647 66 190 948
Fragments arranged by size:
1709 948 647 190 70 66
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 628 651 660 683 754 786 814 846
Size: 628 23 9 23 71 32 28 32
Cuts at: 846 847 879 961 993 2923 2955 3015 3047
Size: 1 32 82 32 1930 32 60 32
Cuts at: 3047 3630
Size: 583
Fragments arranged by size:
1930 628 583 82 71 60 32 32
32 32 32 32 28 23 23 9
1
HincII GTy'rAC
Cuts at: 0 2334 2541 2814 3624 3630
Size: 2334 207 273 810 6
Fragments arranged by size:
2334 810 273 207 6
KasI G'GCGC_C
Cuts at: 0 1775 2422 3630
Size: 1775 647 1208
Fragments arranged by size:
1775 1208 647
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 689 878 1991 2708 2745 2972 3630
Size: 689 189 1113 717 37 227 658
Fragments arranged by size:
1113 717 689 658 227 189 37
MscI TGG'CCA
Cuts at: 0 673 1342 3630
Size: 673 669 2288
Fragments arranged by size:
2288 673 669
MslI CAynn'nnrTG
Cuts at: 0 2395 2926 3109 3341 3630
Size: 2395 531 183 232 289
Fragments arranged by size:
2395 531 289 232 183
MspA1I CmG'CkG
Cuts at: 0 25 55 1095 1280 1578 2154 3036 3630
Size: 25 30 1040 185 298 576 882 594
Fragments arranged by size:
1040 882 594 576 298 185 30 25
NaeI GCC'GGC
Cuts at: 0 1015 1674 3630
Size: 1015 659 1956
Fragments arranged by size:
1956 1015 659
NarI GG'CG_CC
Cuts at: 0 1776 2423 3630
Size: 1776 647 1207
Fragments arranged by size:
1776 1207 647
NcoI C'CATG_G
Cuts at: 0 1051 2547 3630
Size: 1051 1496 1083
Fragments arranged by size:
1496 1083 1051
NgoMIV G'CCGG_C
Cuts at: 0 1013 1672 3630
Size: 1013 659 1958
Fragments arranged by size:
1958 1013 659
NotI GC'GGCC_GC
Cuts at: 0 32 3630
Size: 32 3598
NspI r_CATG'y
Cuts at: 0 2071 2409 3630
Size: 2071 338 1221
Fragments arranged by size:
2071 1221 338
PasI CC'CwG_GG
Cuts at: 0 471 3630
Size: 471 3159
PflMI CCAn_nnn'nTGG
Cuts at: 0 265 438 574 648 2369 3630
Size: 265 173 136 74 1721 1261
Fragments arranged by size:
1721 1261 265 173 136 74
PfoI T'CCnGG_A
Cuts at: 0 1517 1913 3008 3630
Size: 1517 396 1095 622
Fragments arranged by size:
1517 1095 622 396
PmlI CAC'GTG
Cuts at: 0 2617 2924 3630
Size: 2617 307 706
Fragments arranged by size:
2617 706 307
PpuMI rG'GwC_Cy
Cuts at: 0 300 762 770 1158 1169 1193 2664 3182
Size: 300 462 8 388 11 24 1471 518
Cuts at: 3182 3630
Size: 448
Fragments arranged by size:
1471 518 462 448 388 300 24 11
8
PshAI GACnn'nnGTC
Cuts at: 0 809 3630
Size: 809 2821
PspOMI G'GGCC_C
Cuts at: 0 1819 3630
Size: 1819 1811
PstI C_TGCA'G
Cuts at: 0 24 483 1683 1991 2117 2379 2915 3167
Size: 24 459 1200 308 126 262 536 252
Cuts at: 3167 3630
Size: 463
Fragments arranged by size:
1200 536 463 459 308 262 252 126
24
PvuI CG_AT'CG
Cuts at: 0 2895 3630
Size: 2895 735
PvuII CAG'CTG
Cuts at: 0 1280 1578 3630
Size: 1280 298 2052
Fragments arranged by size:
2052 1280 298
SacI G_AGCT'C
Cuts at: 0 3605 3630
Size: 3605 25
SacII CC_GC'GG
Cuts at: 0 1096 3630
Size: 1096 2534
SanDI GG'GwC_CC
Cuts at: 0 762 770 1169 1193 3630
Size: 762 8 399 24 2437
Fragments arranged by size:
2437 762 399 24 8
SfcI C'TryA_G
Cuts at: 0 20 245 479 1679 1987 2113 2232 2375
Size: 20 225 234 1200 308 126 119 143
Cuts at: 2375 2911 3118 3163 3630
Size: 536 207 45 467
Fragments arranged by size:
1200 536 467 308 234 225 207 143
126 119 45 20
SfoI GGC'GCC
Cuts at: 0 1777 2424 3630
Size: 1777 647 1206
Fragments arranged by size:
1777 1206 647
SmaI CCC'GGG
Cuts at: 0 768 1484 1648 1719 3630
Size: 768 716 164 71 1911
Fragments arranged by size:
1911 768 716 164 71
SmlI C'TyrA_G
Cuts at: 0 286 1390 1628 3630
Size: 286 1104 238 2002
Fragments arranged by size:
2002 1104 286 238
SphI G_CATG'C
Cuts at: 0 2071 2409 3630
Size: 2071 338 1221
Fragments arranged by size:
2071 1221 338
SrfI GCCC'GGGC
Cuts at: 0 1484 3630
Size: 1484 2146
SspI AAT'ATT
Cuts at: 0 3493 3630
Size: 3493 137
StuI AGG'CCT
Cuts at: 0 1564 3630
Size: 1564 2066
StyI C'CwwG_G
Cuts at: 0 155 533 1051 1100 2547 3630
Size: 155 378 518 49 1447 1083
Fragments arranged by size:
1447 1083 518 378 155 49
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 787 1044 1311 2920 3630
Size: 787 257 267 1609 710
Fragments arranged by size:
1609 787 710 267 257
TatI w'GTAC_w
Cuts at: 0 1148 2852 3287 3630
Size: 1148 1704 435 343
Fragments arranged by size:
1704 1148 435 343
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1668 1700 2458 2490 3630
Size: 1668 32 758 32 1140
Fragments arranged by size:
1668 1140 758 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1149 1350 3630
Size: 1149 201 2280
Fragments arranged by size:
2280 1149 201
XmaI C'CCGG_G
Cuts at: 0 766 1482 1646 1717 3630
Size: 766 716 164 71 1913
Fragments arranged by size:
1913 766 716 164 71
XmnI GAAnn'nnTTC
Cuts at: 0 2507 3630
Size: 2507 1123
ZraI GAC'GTC
Cuts at: 0 2331 2430 2876 3630
Size: 2331 99 446 754
Fragments arranged by size:
2331 754 446 99
Enzymes that do cut:
AarI AatII AcuI AfeI AflIII AhdI AleI AlfI
AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspMI BsrDI BsrFI BssHII BssSI
BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NspI PasI
PflMI PfoI PmlI PpuMI PshAI PspOMI PstI PvuI
PvuII SacI SacII SanDI SfcI SfoI SmaI SmlI
SphI SrfI SspI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AflII AgeI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BciVI BclI BfrBI BmtI
BsaBI BsiWI BspHI BsrBI BsrGI BstBI BstZ17I CspCI
DraI DraIII DrdI EcoRI EcoRV FalI FseI FspI
FspAI HindIII HpaI KpnI MfeI MluI NdeI NheI
NruI NsiI PacI PciI PmeI PpiI PsiI PspXI
PsrI RsrII SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SnaBI SpeI SwaI Tth111I XbaI XhoI