Restriction map for
pFN21AE9253
(Linear) (Six-base) MAPSORT of: KIEE9253.seq Check: 7762 from: 1 to: 9342
KIEE9253.orf
With 182 enzymes: SgfI *
February 3, 2015 16:28 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 8847 9342
Size: 8847 495
AccI GT'mk_AC
Cuts at: 0 1069 7575 9342
Size: 1069 6506 1767
Fragments arranged by size:
6506 1767 1069
Acc65I G'GTAC_C
Cuts at: 0 5271 9342
Size: 5271 4071
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 109 1687 1972 2200 2408 3296 3655
Size: 34 75 1578 285 228 208 888 359
Cuts at: 3655 3850 5018 5210 5609 6031 6766 6991 7487
Size: 195 1168 192 399 422 735 225 496
Cuts at: 7487 8768 9342
Size: 1281 574
Fragments arranged by size:
1578 1281 1168 888 735 574 496 422
399 359 285 228 225 208 195 192
75 34
AfeI AGC'GCT
Cuts at: 0 9062 9342
Size: 9062 280
AflII C'TTAA_G
Cuts at: 0 1471 2706 2935 4947 5503 6454 7000 9342
Size: 1471 1235 229 2012 556 951 546 2342
Fragments arranged by size:
2342 2012 1471 1235 951 556 546 229
AflIII A'CryG_T
Cuts at: 0 2051 9342
Size: 2051 7291
AgeI A'CCGG_T
Cuts at: 0 2249 9342
Size: 2249 7093
AhdI GACnn_n'nnGTC
Cuts at: 0 674 761 1056 2064 3759 7133 7812 9342
Size: 674 87 295 1008 1695 3374 679 1530
Fragments arranged by size:
3374 1695 1530 1008 679 674 295 87
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4575 4606 9342
Size: 4575 31 4736
Fragments arranged by size:
4736 4575 31
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 941 973 1382 1414 9342
Size: 941 32 409 32 7928
Fragments arranged by size:
7928 941 409 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 2223 3294 4836 6138 7548 7956 8229 8488
Size: 2223 1071 1542 1302 1410 408 273 259
Cuts at: 8488 9342
Size: 854
Fragments arranged by size:
2223 1542 1410 1302 1071 854 408 273
259
ApoI r'AATT_y
Cuts at: 0 56 291 312 440 1560 2469 2506 3147
Size: 56 235 21 128 1120 909 37 641
Cuts at: 3147 3250 3599 4102 4228 4256 4335 4739 5346
Size: 103 349 503 126 28 79 404 607
Cuts at: 5346 6534 7080 7242 7589 9342
Size: 1188 546 162 347 1753
Fragments arranged by size:
1753 1188 1120 909 641 607 546 503
404 349 347 235 162 128 126 103
79 56 37 28 21
AseI AT'TA_AT
Cuts at: 0 881 985 1931 2945 9342
Size: 881 104 946 1014 6397
Fragments arranged by size:
6397 1014 946 881 104
AvaI C'yCGr_G
Cuts at: 0 130 4687 4750 8690 9342
Size: 130 4557 63 3940 652
Fragments arranged by size:
4557 3940 652 130 63
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 8489 8522 9342
Size: 8489 33 820
Fragments arranged by size:
8489 820 33
BanI G'GyrC_C
Cuts at: 0 5271 9175 9342
Size: 5271 3904 167
Fragments arranged by size:
5271 3904 167
BanII G_rGCy'C
Cuts at: 0 48 1199 4288 4415 4494 4751 5224 6325
Size: 48 1151 3089 127 79 257 473 1101
Cuts at: 6325 6924 7349 8000 8267 9163 9342
Size: 599 425 651 267 896 179
Fragments arranged by size:
3089 1151 1101 896 651 599 473 425
267 257 179 127 79 48
BbsI GAAGACnn'nnnn_
Cuts at: 0 673 1960 2176 2675 2819 3487 4054 4091
Size: 673 1287 216 499 144 668 567 37
Cuts at: 4091 8912 9342
Size: 4821 430
Fragments arranged by size:
4821 1287 673 668 567 499 430 216
144 37
BbvCI CC'TCA_GC
Cuts at: 0 1429 9342
Size: 1429 7913
BciVI GTATCCnnnnn_n'
Cuts at: 0 8490 9342
Size: 8490 852
BclI T'GATC_A
Cuts at: 0 2361 3045 4266 5553 8393 9342
Size: 2361 684 1221 1287 2840 949
Fragments arranged by size:
2840 2361 1287 1221 949 684
BfrBI ATG'CAT
Cuts at: 0 2379 2619 2950 3103 5234 5474 5616 7875
Size: 2379 240 331 153 2131 240 142 2259
Cuts at: 7875 9342
Size: 1467
Fragments arranged by size:
2379 2259 2131 1467 331 240 240 153
142
BglI GCCn_nnn'nGGC
Cuts at: 0 565 6280 7393 9342
Size: 565 5715 1113 1949
Fragments arranged by size:
5715 1949 1113 565
BglII A'GATC_T
Cuts at: 0 495 1614 2199 3331 3729 9342
Size: 495 1119 585 1132 398 5613
Fragments arranged by size:
5613 1132 1119 585 495 398
BlpI GC'TnA_GC
Cuts at: 0 5093 6319 9152 9342
Size: 5093 1226 2833 190
Fragments arranged by size:
5093 2833 1226 190
Bme1580I G_kGCm'C
Cuts at: 0 2715 8373 9342
Size: 2715 5658 969
Fragments arranged by size:
5658 2715 969
BmrI ACTGGGnnnn_n'
Cuts at: 0 2195 9157 9205 9342
Size: 2195 6962 48 137
Fragments arranged by size:
6962 2195 137 48
BmtI G_CTAG'C
Cuts at: 0 5738 7939 9342
Size: 5738 2201 1403
Fragments arranged by size:
5738 2201 1403
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1215 9342
Size: 1215 8127
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 256 985 1534 2023 4873 4912 5183 5554
Size: 256 729 549 489 2850 39 271 371
Cuts at: 5554 5782 6082 7570 7961 8251 9328 9342
Size: 228 300 1488 391 290 1077 14
Fragments arranged by size:
2850 1488 1077 729 549 489 391 371
300 290 271 256 228 39 14
Bpu10I CC'TnA_GC
Cuts at: 0 1429 2851 9342
Size: 1429 1422 6491
Fragments arranged by size:
6491 1429 1422
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1510 1694 2737 4675 4927 5992 7090 7459
Size: 1510 184 1043 1938 252 1065 1098 369
Cuts at: 7459 9342
Size: 1883
Fragments arranged by size:
1938 1883 1510 1098 1065 1043 369 252
184
BsaI GGTCTCn'nnnn_
Cuts at: 0 2074 7093 8702 9342
Size: 2074 5019 1609 640
Fragments arranged by size:
5019 2074 1609 640
BsaAI yAC'GTr
Cuts at: 0 5635 9342
Size: 5635 3707
BsaBI GATnn'nnATC
Cuts at: 0 5069 6745 9342
Size: 5069 1676 2597
Fragments arranged by size:
5069 2597 1676
BsaHI Gr'CG_yC
Cuts at: 0 1057 9342
Size: 1057 8285
BsaWI w'CCGG_w
Cuts at: 0 2249 3094 9342
Size: 2249 845 6248
Fragments arranged by size:
6248 2249 845
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 941 971 2424 2454 3679 3709 4846 4852
Size: 941 30 1453 30 1225 30 1137 6
Cuts at: 4852 4876 4882 5639 5669 9342
Size: 24 6 757 30 3673
Fragments arranged by size:
3673 1453 1225 1137 941 757 30 30
30 30 24 6 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1207 1446 2445 3740 4420 4467 4612 9342
Size: 1207 239 999 1295 680 47 145 4730
Fragments arranged by size:
4730 1295 1207 999 680 239 145 47
BseYI C'CCAG_C
Cuts at: 0 5 4395 4632 9289 9342
Size: 5 4390 237 4657 53
Fragments arranged by size:
4657 4390 237 53 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1401 3337 4581 5159 6391 8447 9032 9342
Size: 1401 1936 1244 578 1232 2056 585 310
Fragments arranged by size:
2056 1936 1401 1244 1232 585 578 310
BsiHKAI G_wGCw'C
Cuts at: 0 48 1199 3874 4175 4288 4494 4751 5164
Size: 48 1151 2675 301 113 206 257 413
Cuts at: 5164 6325 7349 7643 8000 8267 9342
Size: 1161 1024 294 357 267 1075
Fragments arranged by size:
2675 1161 1151 1075 1024 413 357 301
294 267 257 206 113 48
BsmI GAATG_Cn'
Cuts at: 0 3166 3181 5236 6002 9342
Size: 3166 15 2055 766 3340
Fragments arranged by size:
3340 3166 2055 766 15
Bsp1286I G_dGCh'C
Cuts at: 0 48 1199 2715 3874 4175 4288 4415 4494
Size: 48 1151 1516 1159 301 113 127 79
Cuts at: 4494 4751 5164 5224 6325 6924 7349 7643 8000
Size: 257 413 60 1101 599 425 294 357
Cuts at: 8000 8267 8373 9163 9342
Size: 267 106 790 179
Fragments arranged by size:
1516 1159 1151 1101 790 599 425 413
357 301 294 267 257 179 127 113
106 79 60 48
BspHI T'CATG_A
Cuts at: 0 261 1974 2600 3395 3828 8109 8882 9342
Size: 261 1713 626 795 433 4281 773 460
Fragments arranged by size:
4281 1713 795 773 626 460 433 261
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2552 8847 9342
Size: 2552 6295 495
Fragments arranged by size:
6295 2552 495
BsrBI CCG'CTC
Cuts at: 0 6438 9342
Size: 6438 2904
BsrDI GCAATG_nn'
Cuts at: 0 617 5997 9342
Size: 617 5380 3345
Fragments arranged by size:
5380 3345 617
BsrFI r'CCGG_y
Cuts at: 0 2249 4676 9342
Size: 2249 2427 4666
Fragments arranged by size:
4666 2427 2249
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1138 3630 3756 9342
Size: 1138 2492 126 5586
Fragments arranged by size:
5586 2492 1138 126
BstEII G'GTnAC_C
Cuts at: 0 6829 9342
Size: 6829 2513
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2926 5890 5928 6276 9342
Size: 2926 2964 38 348 3066
Fragments arranged by size:
3066 2964 2926 348 38
BstYI r'GATC_y
Cuts at: 0 495 1614 2199 2292 3111 3331 3582 3729
Size: 495 1119 585 93 819 220 251 147
Cuts at: 3729 7703 8800 9342
Size: 3974 1097 542
Fragments arranged by size:
3974 1119 1097 819 585 542 495 251
220 147 93
BstZ17I GTA'TAC
Cuts at: 0 1070 9342
Size: 1070 8272
Bsu36I CC'TnA_GG
Cuts at: 0 2287 4792 9262 9342
Size: 2287 2505 4470 80
Fragments arranged by size:
4470 2505 2287 80
BtgI C'CryG_G
Cuts at: 0 4068 5921 7316 9017 9342
Size: 4068 1853 1395 1701 325
Fragments arranged by size:
4068 1853 1701 1395 325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 6396 9342
Size: 6396 2946
BtsI GCAGTG_nn'
Cuts at: 0 2560 4569 5147 6776 7310 9327 9342
Size: 2560 2009 578 1629 534 2017 15
Fragments arranged by size:
2560 2017 2009 1629 578 534 15
DraI TTT'AAA
Cuts at: 0 1547 2747 2891 3383 3404 3570 6708 8246
Size: 1547 1200 144 492 21 166 3138 1538
Cuts at: 8246 9342
Size: 1096
Fragments arranged by size:
3138 1547 1538 1200 1096 492 166 144
21
DraIII CAC_nnn'GTG
Cuts at: 0 2052 4134 9342
Size: 2052 2082 5208
Fragments arranged by size:
5208 2082 2052
EaeI y'GGCC_r
Cuts at: 0 5657 6581 8769 9342
Size: 5657 924 2188 573
Fragments arranged by size:
5657 2188 924 573
EarI CTCTTCn'nnn_
Cuts at: 0 53 593 1355 1835 2555 2903 4004 4643
Size: 53 540 762 480 720 348 1101 639
Cuts at: 4643 4694 5229 6348 6569 6705 6878 8020 8255
Size: 51 535 1119 221 136 173 1142 235
Cuts at: 8255 8411 9342
Size: 156 931
Fragments arranged by size:
1142 1119 1101 931 762 720 639 540
535 480 348 235 221 173 156 136
53 51
EcoICRI GAG'CTC
Cuts at: 0 46 1197 4286 4492 4749 6323 7347 7998
Size: 46 1151 3089 206 257 1574 1024 651
Cuts at: 7998 8265 9342
Size: 267 1077
Fragments arranged by size:
3089 1574 1151 1077 1024 651 267 257
206 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 109 256 985 1534 1687 1972 2023
Size: 34 75 147 729 549 153 285 51
Cuts at: 2023 2200 2408 3296 3655 3850 4873 4912 5018
Size: 177 208 888 359 195 1023 39 106
Cuts at: 5018 5183 5210 5554 5609 5782 6031 6082 6766
Size: 165 27 344 55 173 249 51 684
Cuts at: 6766 6991 7487 7570 7961 8251 8768 9328 9342
Size: 225 496 83 391 290 517 560 14
Fragments arranged by size:
1023 888 729 684 560 549 517 496
391 359 344 290 285 249 225 208
195 177 173 165 153 147 106 83
75 55 51 51 39 34 27 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 4666 7468 8705 9342
Size: 4666 2802 1237 637
Fragments arranged by size:
4666 2802 1237 637
EcoO109I rG'GnC_Cy
Cuts at: 0 3823 5100 9258 9342
Size: 3823 1277 4158 84
Fragments arranged by size:
4158 3823 1277 84
EcoRI G'AATT_C
Cuts at: 0 312 3147 3250 6534 7080 9342
Size: 312 2835 103 3284 546 2262
Fragments arranged by size:
3284 2835 2262 546 312 103
EcoRV GAT'ATC
Cuts at: 0 5304 9342
Size: 5304 4038
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 94 1932 2438 2991 3082 6217 7624 8563
Size: 94 1838 506 553 91 3135 1407 939
Cuts at: 8563 9109 9342
Size: 546 233
Fragments arranged by size:
3135 1838 1407 939 553 546 506 233
94 91
FspI TGC'GCA
Cuts at: 0 395 3623 9342
Size: 395 3228 5719
Fragments arranged by size:
5719 3228 395
HaeII r_GCGC'y
Cuts at: 0 9064 9342
Size: 9064 278
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 660 692 747 779 1042 1074 1765 1797
Size: 660 32 55 32 263 32 691 32
Cuts at: 1797 2050 2082 2424 2456 2787 2819 3745 3777
Size: 253 32 342 32 331 32 926 32
Cuts at: 3777 3958 3990 4642 4674 4846 4878 5614 5646
Size: 181 32 652 32 172 32 736 32
Cuts at: 5646 5893 5923 5925 5955 5959 5991 7119 7151
Size: 247 30 2 30 4 32 1128 32
Cuts at: 7151 7207 7239 7798 7830 8313 8345 9342
Size: 56 32 559 32 483 32 997
Fragments arranged by size:
1128 997 926 736 691 660 652 559
483 342 331 263 253 247 181 172
56 55 32 32 32 32 32 32
32 32 32 32 32 32 32 32
32 32 32 30 30 4 2
HincII GTy'rAC
Cuts at: 0 1061 1207 3839 4317 4349 9342
Size: 1061 146 2632 478 32 4993
Fragments arranged by size:
4993 2632 1061 478 146 32
HindIII A'AGCT_T
Cuts at: 0 1669 2212 2853 8780 9342
Size: 1669 543 641 5927 562
Fragments arranged by size:
5927 1669 641 562 543
KpnI G_GTAC'C
Cuts at: 0 5275 9342
Size: 5275 4067
MfeI C'AATT_G
Cuts at: 0 7987 9342
Size: 7987 1355
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 869 978 984 1843 3191 3287 7940 9262
Size: 869 109 6 859 1348 96 4653 1322
Cuts at: 9262 9338 9342
Size: 76 4
Fragments arranged by size:
4653 1348 1322 869 859 109 96 76
6 4
MscI TGG'CCA
Cuts at: 0 5659 6583 8771 9342
Size: 5659 924 2188 571
Fragments arranged by size:
5659 2188 924 571
MslI CAynn'nnrTG
Cuts at: 0 1246 2687 5984 6007 7315 7321 9342
Size: 1246 1441 3297 23 1308 6 2021
Fragments arranged by size:
3297 2021 1441 1308 1246 23 6
MspA1I CmG'CkG
Cuts at: 0 3465 4395 5675 6348 6405 6894 8412 9118
Size: 3465 930 1280 673 57 489 1518 706
Cuts at: 9118 9156 9342
Size: 38 186
Fragments arranged by size:
3465 1518 1280 930 706 673 489 186
57 38
NcoI C'CATG_G
Cuts at: 0 4068 7316 9017 9342
Size: 4068 3248 1701 325
Fragments arranged by size:
4068 3248 1701 325
NdeI CA'TA_TG
Cuts at: 0 3105 9342
Size: 3105 6237
NheI G'CTAG_C
Cuts at: 0 5734 7935 9342
Size: 5734 2201 1407
Fragments arranged by size:
5734 2201 1407
NsiI A_TGCA'T
Cuts at: 0 2381 2621 2952 3105 5236 5476 5618 7877
Size: 2381 240 331 153 2131 240 142 2259
Cuts at: 7877 9342
Size: 1465
Fragments arranged by size:
2381 2259 2131 1465 331 240 240 153
142
NspI r_CATG'y
Cuts at: 0 2272 2421 3759 5474 5620 7063 8043 9342
Size: 2272 149 1338 1715 146 1443 980 1299
Fragments arranged by size:
2272 1715 1443 1338 1299 980 149 146
PasI CC'CwG_GG
Cuts at: 0 5217 8373 9342
Size: 5217 3156 969
Fragments arranged by size:
5217 3156 969
PflMI CCAn_nnn'nTGG
Cuts at: 0 1227 9118 9342
Size: 1227 7891 224
Fragments arranged by size:
7891 1227 224
PfoI T'CCnGG_A
Cuts at: 0 296 6098 8721 9342
Size: 296 5802 2623 621
Fragments arranged by size:
5802 2623 621 296
PmlI CAC'GTG
Cuts at: 0 5635 9342
Size: 5635 3707
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 941 973 9342
Size: 941 32 8369
Fragments arranged by size:
8369 941 32
PpuMI rG'GwC_Cy
Cuts at: 0 5100 9342
Size: 5100 4242
PshAI GACnn'nnGTC
Cuts at: 0 2459 4058 9309 9342
Size: 2459 1599 5251 33
Fragments arranged by size:
5251 2459 1599 33
PsiI TTA'TAA
Cuts at: 0 3617 8605 9342
Size: 3617 4988 737
Fragments arranged by size:
4988 3617 737
PspXI vC'TCGA_Gb
Cuts at: 0 4750 9342
Size: 4750 4592
PstI C_TGCA'G
Cuts at: 0 146 405 1307 3704 3932 4307 4595 6113
Size: 146 259 902 2397 228 375 288 1518
Cuts at: 6113 6227 6771 7760 7865 8438 8904 9342
Size: 114 544 989 105 573 466 438
Fragments arranged by size:
2397 1518 989 902 573 544 466 438
375 288 259 228 146 114 105
PvuII CAG'CTG
Cuts at: 0 3465 4395 5675 6348 6405 6894 8412 9118
Size: 3465 930 1280 673 57 489 1518 706
Cuts at: 9118 9342
Size: 224
Fragments arranged by size:
3465 1518 1280 930 706 673 489 224
57
SacI G_AGCT'C
Cuts at: 0 48 1199 4288 4494 4751 6325 7349 8000
Size: 48 1151 3089 206 257 1574 1024 651
Cuts at: 8000 8267 9342
Size: 267 1075
Fragments arranged by size:
3089 1574 1151 1075 1024 651 267 257
206 48
SapI GCTCTTCn'nnn_
Cuts at: 0 53 2903 4643 5229 6878 8020 8255 9342
Size: 53 2850 1740 586 1649 1142 235 1087
Fragments arranged by size:
2850 1740 1649 1142 1087 586 235 53
SbfI CC_TGCA'GG
Cuts at: 0 1307 6227 9342
Size: 1307 4920 3115
Fragments arranged by size:
4920 3115 1307
ScaI AGT'ACT
Cuts at: 0 8590 9342
Size: 8590 752
SexAI A'CCwGG_T
Cuts at: 0 8051 9342
Size: 8051 1291
SfcI C'TryA_G
Cuts at: 0 142 401 1303 2107 3700 3928 4303 4591
Size: 142 259 902 804 1593 228 375 288
Cuts at: 4591 5042 6109 6223 6767 7756 7861 8434 8900
Size: 451 1067 114 544 989 105 573 466
Cuts at: 8900 9342
Size: 442
Fragments arranged by size:
1593 1067 989 902 804 573 544 466
451 442 375 288 259 228 142 114
105
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 6280 9342
Size: 6280 3062
SmlI C'TyrA_G
Cuts at: 0 130 1471 1525 1709 2706 2716 2935 4654
Size: 130 1341 54 184 997 10 219 1719
Cuts at: 4654 4687 4750 4906 4947 5503 5971 6454 7000
Size: 33 63 156 41 556 468 483 546
Cuts at: 7000 7069 7438 9342
Size: 69 369 1904
Fragments arranged by size:
1904 1719 1341 997 556 546 483 468
369 219 184 156 130 69 63 54
41 33 10
SpeI A'CTAG_T
Cuts at: 0 1026 3181 9342
Size: 1026 2155 6161
Fragments arranged by size:
6161 2155 1026
SphI G_CATG'C
Cuts at: 0 2272 2421 5474 5620 7063 8043 9342
Size: 2272 149 3053 146 1443 980 1299
Fragments arranged by size:
3053 2272 1443 1299 980 149 146
SspI AAT'ATT
Cuts at: 0 472 4043 5542 7260 9342
Size: 472 3571 1499 1718 2082
Fragments arranged by size:
3571 2082 1718 1499 472
StuI AGG'CCT
Cuts at: 0 5594 6274 7463 9342
Size: 5594 680 1189 1879
Fragments arranged by size:
5594 1879 1189 680
StyI C'CwwG_G
Cuts at: 0 4068 5660 6269 6275 7316 8501 8561 9017
Size: 4068 1592 609 6 1041 1185 60 456
Cuts at: 9017 9342
Size: 325
Fragments arranged by size:
4068 1592 1185 1041 609 456 325 60
6
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1279 4946 9342
Size: 1279 3667 4396
Fragments arranged by size:
4396 3667 1279
TatI w'GTAC_w
Cuts at: 0 3050 7287 8588 9342
Size: 3050 4237 1301 754
Fragments arranged by size:
4237 3050 1301 754
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1222 1254 3679 3711 5639 5671 9342
Size: 1222 32 2425 32 1928 32 3671
Fragments arranged by size:
3671 2425 1928 1222 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4182 6054 6613 9342
Size: 4182 1872 559 2729
Fragments arranged by size:
4182 2729 1872 559
XbaI T'CTAG_A
Cuts at: 0 2202 3504 3606 5109 6864 7776 9342
Size: 2202 1302 102 1503 1755 912 1566
Fragments arranged by size:
2202 1755 1566 1503 1302 912 102
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1149 9039 9303 9342
Size: 1149 7890 264 39
Fragments arranged by size:
7890 1149 264 39
XhoI C'TCGA_G
Cuts at: 0 130 4687 4750 9342
Size: 130 4557 63 4592
Fragments arranged by size:
4592 4557 130 63
XmnI GAAnn'nnTTC
Cuts at: 0 6044 9342
Size: 6044 3298
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflII AflIII AgeI
AhdI AlfI AloI AlwNI ApoI AseI AvaI BaeI
BanI BanII BbsI BbvCI BciVI BclI BfrBI BglI
BglII BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrFI BstAPI BstEII BstXI BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI DraI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII
Hin4I HincII HindIII KpnI MfeI MmeI MscI MslI
MspA1I NcoI NdeI NheI NsiI NspI PasI PflMI
PfoI PmlI PpiI PpuMI PshAI PsiI PspXI PstI
PvuII SacI SapI SbfI ScaI SexAI SfcI SfiI
SmlI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AclI AleI ApaI ApaLI AscI AsiSI AvrII
BamHI BbeI BcgI BmgBI BsiEI BsiWI BsmBI BspEI
BsrGI BssHII BssSI BstBI ClaI CspCI DrdI EagI
EciI FseI FspAI HpaI KasI MluI NaeI NarI
NgoMIV NotI NruI PacI PciI PmeI PspOMI PsrI
PvuI RsrII SacII SalI SanDI SfoI SgfI SgrAI
SmaI SnaBI SrfI SwaI XmaI ZraI