Restriction map for
pFN21AE9246
(Linear) (Six-base) MAPSORT of: KIEE9246.seq Check: 5884 from: 1 to: 5010
KIEE9246.orf
With 182 enzymes: SgfI *
November 16, 2016 14:56 ..
AatII G_ACGT'C
Cuts at: 0 3680 5010
Size: 3680 1330
AccI GT'mk_AC
Cuts at: 0 2697 5010
Size: 2697 2313
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 520 1847 1870 3491 3662 4258 4397 4724
Size: 520 1327 23 1621 171 596 139 327
Cuts at: 4724 4757 5010
Size: 33 253
Fragments arranged by size:
1621 1327 596 520 327 253 171 139
33 23
AflII C'TTAA_G
Cuts at: 0 4962 5010
Size: 4962 48
AflIII A'CryG_T
Cuts at: 0 597 833 945 2975 5010
Size: 597 236 112 2030 2035
Fragments arranged by size:
2035 2030 597 236 112
AhdI GACnn_n'nnGTC
Cuts at: 0 4303 5010
Size: 4303 707
AleI CACnn'nnGTG
Cuts at: 0 4223 5010
Size: 4223 787
ApoI r'AATT_y
Cuts at: 0 364 423 1621 1644 1678 1756 1951 2625
Size: 364 59 1198 23 34 78 195 674
Cuts at: 2625 3291 3618 3697 4041 4108 4205 5010
Size: 666 327 79 344 67 97 805
Fragments arranged by size:
1198 805 674 666 364 344 327 195
97 79 78 67 59 34 23
AseI AT'TA_AT
Cuts at: 0 2054 5010
Size: 2054 2956
AvaI C'yCGr_G
Cuts at: 0 359 1460 2888 3548 5010
Size: 359 1101 1428 660 1462
Fragments arranged by size:
1462 1428 1101 660 359
BanI G'GyrC_C
Cuts at: 0 2959 5010
Size: 2959 2051
BanII G_rGCy'C
Cuts at: 0 152 275 5010
Size: 152 123 4735
Fragments arranged by size:
4735 152 123
BbsI GAAGACnn'nnnn_
Cuts at: 0 942 2792 3851 4385 5010
Size: 942 1850 1059 534 625
Fragments arranged by size:
1850 1059 942 625 534
BbvCI CC'TCA_GC
Cuts at: 0 205 2422 5010
Size: 205 2217 2588
Fragments arranged by size:
2588 2217 205
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3304 3338 5010
Size: 3304 34 1672
Fragments arranged by size:
3304 1672 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1390 2268 5010
Size: 1390 878 2742
Fragments arranged by size:
2742 1390 878
BclI T'GATC_A
Cuts at: 0 1197 3522 5010
Size: 1197 2325 1488
Fragments arranged by size:
2325 1488 1197
BfrBI ATG'CAT
Cuts at: 0 544 2359 5010
Size: 544 1815 2651
Fragments arranged by size:
2651 1815 544
BglI GCCn_nnn'nGGC
Cuts at: 0 279 5010
Size: 279 4731
BglII A'GATC_T
Cuts at: 0 1021 5010
Size: 1021 3989
BlpI GC'TnA_GC
Cuts at: 0 146 5010
Size: 146 4864
Bme1580I G_kGCm'C
Cuts at: 0 2593 2962 5010
Size: 2593 369 2048
Fragments arranged by size:
2593 2048 369
BmgBI CAC'GTC
Cuts at: 0 439 2976 5010
Size: 439 2537 2034
Fragments arranged by size:
2537 2034 439
BmrI ACTGGGnnnn_n'
Cuts at: 0 2663 3575 5010
Size: 2663 912 1435
Fragments arranged by size:
2663 1435 912
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2466 5010
Size: 2466 2544
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 1205 3995 4058 4232 5010
Size: 335 870 2790 63 174 778
Fragments arranged by size:
2790 870 778 335 174 63
Bpu10I CC'TnA_GC
Cuts at: 0 205 928 2422 5010
Size: 205 723 1494 2588
Fragments arranged by size:
2588 1494 723 205
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1956 2161 2277 3357 3421 4078 4489
Size: 121 1835 205 116 1080 64 657 411
Cuts at: 4489 4877 5010
Size: 388 133
Fragments arranged by size:
1835 1080 657 411 388 205 133 121
116 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 1382 4588 5010
Size: 1382 3206 422
Fragments arranged by size:
3206 1382 422
BsaAI yAC'GTr
Cuts at: 0 834 4609 5010
Size: 834 3775 401
Fragments arranged by size:
3775 834 401
BsaHI Gr'CG_yC
Cuts at: 0 3677 5010
Size: 3677 1333
BsaWI w'CCGG_w
Cuts at: 0 93 5010
Size: 93 4917
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 88 118 2986 3016 5010
Size: 88 30 2868 30 1994
Fragments arranged by size:
2868 1994 88 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 187 219 306 3010 5010
Size: 187 32 87 2704 2000
Fragments arranged by size:
2704 2000 187 87 32
BseYI C'CCAG_C
Cuts at: 0 151 163 2793 5010
Size: 151 12 2630 2217
Fragments arranged by size:
2630 2217 151 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3241 5010
Size: 3241 1769
BsiEI CG_ry'CG
Cuts at: 0 248 5010
Size: 248 4762
BsiHKAI G_wGCw'C
Cuts at: 0 2401 3731 5010
Size: 2401 1330 1279
Fragments arranged by size:
2401 1330 1279
BsmI GAATG_Cn'
Cuts at: 0 849 2775 3450 4149 5010
Size: 849 1926 675 699 861
Fragments arranged by size:
1926 861 849 699 675
BsmBI CGTCTCn'nnnn_
Cuts at: 0 10 62 4658 5010
Size: 10 52 4596 352
Fragments arranged by size:
4596 352 52 10
Bsp1286I G_dGCh'C
Cuts at: 0 152 275 2401 2593 2962 3731 5010
Size: 152 123 2126 192 369 769 1279
Fragments arranged by size:
2126 1279 769 369 192 152 123
BspEI T'CCGG_A
Cuts at: 0 93 5010
Size: 93 4917
BspHI T'CATG_A
Cuts at: 0 3668 3708 5010
Size: 3668 40 1302
Fragments arranged by size:
3668 1302 40
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3844 5010
Size: 3844 1166
BsrDI GCAATG_nn'
Cuts at: 0 707 2291 3848 4096 5010
Size: 707 1584 1557 248 914
Fragments arranged by size:
1584 1557 914 707 248
BssHII G'CGCG_C
Cuts at: 0 2344 5010
Size: 2344 2666
BssSI C'ACGA_G
Cuts at: 0 211 1136 5010
Size: 211 925 3874
Fragments arranged by size:
3874 925 211
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3006 5010
Size: 3006 2004
BstXI CCAn_nnnn'nTGG
Cuts at: 0 118 2586 5010
Size: 118 2468 2424
Fragments arranged by size:
2468 2424 118
BstYI r'GATC_y
Cuts at: 0 302 1021 2145 2619 5010
Size: 302 719 1124 474 2391
Fragments arranged by size:
2391 1124 719 474 302
Bsu36I CC'TnA_GG
Cuts at: 0 221 2388 5010
Size: 221 2167 2622
Fragments arranged by size:
2622 2167 221
BtgI C'CryG_G
Cuts at: 0 638 761 5010
Size: 638 123 4249
Fragments arranged by size:
4249 638 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 126 5010
Size: 126 4884
BtsI GCAGTG_nn'
Cuts at: 0 3007 3041 5010
Size: 3007 34 1969
Fragments arranged by size:
3007 1969 34
ClaI AT'CG_AT
Cuts at: 0 561 5010
Size: 561 4449
DraI TTT'AAA
Cuts at: 0 723 1091 3890 4398 5010
Size: 723 368 2799 508 612
Fragments arranged by size:
2799 723 612 508 368
DrdI GACnn_nn'nnGTC
Cuts at: 0 950 1455 5010
Size: 950 505 3555
Fragments arranged by size:
3555 950 505
EaeI y'GGCC_r
Cuts at: 0 245 5010
Size: 245 4765
EagI C'GGCC_G
Cuts at: 0 245 5010
Size: 245 4765
EarI CTCTTCn'nnn_
Cuts at: 0 263 307 486 2125 2207 2411 2720 3092
Size: 263 44 179 1639 82 204 309 372
Cuts at: 3092 3713 4505 4700 5010
Size: 621 792 195 310
Fragments arranged by size:
1639 792 621 372 310 309 263 204
195 179 82 44
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 80 5010
Size: 80 4930
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 335 520 1205 1847 1870 3491 3662 3995
Size: 335 185 685 642 23 1621 171 333
Cuts at: 3995 4058 4232 4258 4397 4724 4757 5010
Size: 63 174 26 139 327 33 253
Fragments arranged by size:
1621 685 642 335 333 327 253 185
174 171 139 63 33 26 23
EcoO109I rG'GnC_Cy
Cuts at: 0 262 5010
Size: 262 4748
EcoRI G'AATT_C
Cuts at: 0 1621 5010
Size: 1621 3389
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1102 1120 4456 5000 5010
Size: 1102 18 3336 544 10
Fragments arranged by size:
3336 1102 544 18 10
HaeII r_GCGC'y
Cuts at: 0 91 164 1640 5010
Size: 91 73 1476 3370
Fragments arranged by size:
3370 1476 91 73
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 88 120 543 575 1182 1214 1416 1448
Size: 88 32 423 32 607 32 202 32
Cuts at: 1448 1596 1628 2536 2568 3421 3453 3507 3539
Size: 148 32 908 32 853 32 54 32
Cuts at: 3539 3968 4000 4289 4321 4462 4494 4504 4528
Size: 429 32 289 32 141 32 10 24
Cuts at: 4528 4536 4560 4633 4665 5010
Size: 8 24 73 32 345
Fragments arranged by size:
908 853 607 429 423 345 289 202
148 141 88 73 54 32 32 32
32 32 32 32 32 32 32 32
32 24 24 10 8
HincII GTy'rAC
Cuts at: 0 2379 2981 4166 5010
Size: 2379 602 1185 844
Fragments arranged by size:
2379 1185 844 602
HindIII A'AGCT_T
Cuts at: 0 2137 4932 5010
Size: 2137 2795 78
Fragments arranged by size:
2795 2137 78
MfeI C'AATT_G
Cuts at: 0 4221 5010
Size: 4221 789
MslI CAynn'nnrTG
Cuts at: 0 2207 2354 2438 4223 4330 4832 5010
Size: 2207 147 84 1785 107 502 178
Fragments arranged by size:
2207 1785 502 178 147 107 84
MspA1I CmG'CkG
Cuts at: 0 155 2793 5010
Size: 155 2638 2217
Fragments arranged by size:
2638 2217 155
NcoI C'CATG_G
Cuts at: 0 638 761 5010
Size: 638 123 4249
Fragments arranged by size:
4249 638 123
NdeI CA'TA_TG
Cuts at: 0 580 957 1437 1731 3126 4869 5010
Size: 580 377 480 294 1395 1743 141
Fragments arranged by size:
1743 1395 580 480 377 294 141
NotI GC'GGCC_GC
Cuts at: 0 245 5010
Size: 245 4765
NsiI A_TGCA'T
Cuts at: 0 546 2361 5010
Size: 546 1815 2649
Fragments arranged by size:
2649 1815 546
NspI r_CATG'y
Cuts at: 0 601 1474 1563 1810 3358 3499 5010
Size: 601 873 89 247 1548 141 1511
Fragments arranged by size:
1548 1511 873 601 247 141 89
PciI A'CATG_T
Cuts at: 0 597 5010
Size: 597 4413
PflMI CCAn_nnn'nTGG
Cuts at: 0 4450 5010
Size: 4450 560
PmlI CAC'GTG
Cuts at: 0 834 4609 5010
Size: 834 3775 401
Fragments arranged by size:
3775 834 401
PshAI GACnn'nnGTC
Cuts at: 0 2703 5010
Size: 2703 2307
PsiI TTA'TAA
Cuts at: 0 1235 3625 5010
Size: 1235 2390 1385
Fragments arranged by size:
2390 1385 1235
PstI C_TGCA'G
Cuts at: 0 3036 5010
Size: 3036 1974
PvuII CAG'CTG
Cuts at: 0 2793 5010
Size: 2793 2217
SapI GCTCTTCn'nnn_
Cuts at: 0 263 307 2411 5010
Size: 263 44 2104 2599
Fragments arranged by size:
2599 2104 263 44
ScaI AGT'ACT
Cuts at: 0 4071 5010
Size: 4071 939
SexAI A'CCwGG_T
Cuts at: 0 566 2820 3534 5010
Size: 566 2254 714 1476
Fragments arranged by size:
2254 1476 714 566
SfcI C'TryA_G
Cuts at: 0 432 3032 5010
Size: 432 2600 1978
Fragments arranged by size:
2600 1978 432
SmaI CCC'GGG
Cuts at: 0 2890 3550 5010
Size: 2890 660 1460
Fragments arranged by size:
2890 1460 660
SmlI C'TyrA_G
Cuts at: 0 100 359 1460 1971 2140 2256 3372 3400
Size: 100 259 1101 511 169 116 1116 28
Cuts at: 3400 4057 4468 4892 4962 5010
Size: 657 411 424 70 48
Fragments arranged by size:
1116 1101 657 511 424 411 259 169
116 100 70 48 28
SphI G_CATG'C
Cuts at: 0 1563 1810 5010
Size: 1563 247 3200
Fragments arranged by size:
3200 1563 247
SspI AAT'ATT
Cuts at: 0 384 5010
Size: 384 4626
StuI AGG'CCT
Cuts at: 0 25 282 2872 5010
Size: 25 257 2590 2138
Fragments arranged by size:
2590 2138 257 25
StyI C'CwwG_G
Cuts at: 0 638 761 3281 5010
Size: 638 123 2520 1729
Fragments arranged by size:
2520 1729 638 123
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 631 694 754 1406 2074 5010
Size: 631 63 60 652 668 2936
Fragments arranged by size:
2936 668 652 631 63 60
TatI w'GTAC_w
Cuts at: 0 3071 3133 4069 5010
Size: 3071 62 936 941
Fragments arranged by size:
3071 941 936 62
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 584 616 2064 2096 2790 2822 2986 3018
Size: 584 32 1448 32 694 32 164 32
Cuts at: 3018 5010
Size: 1992
Fragments arranged by size:
1992 1448 694 584 164 32 32 32
32
Tth111I GACn'n_nGTC
Cuts at: 0 2374 5010
Size: 2374 2636
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3507 4082 5010
Size: 3507 575 928
Fragments arranged by size:
3507 928 575
XhoI C'TCGA_G
Cuts at: 0 359 1460 5010
Size: 359 1101 3550
Fragments arranged by size:
3550 1101 359
XmaI C'CCGG_G
Cuts at: 0 2888 3548 5010
Size: 2888 660 1462
Fragments arranged by size:
2888 1462 660
XmnI GAAnn'nnTTC
Cuts at: 0 4001 4439 4909 5010
Size: 4001 438 470 101
Fragments arranged by size:
4001 470 438 101
ZraI GAC'GTC
Cuts at: 0 3678 5010
Size: 3678 1332
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AhdI AleI ApoI
AseI AvaI BanI BanII BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspHI BspMI BsrDI BssHII BssSI BstAPI
BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI DraI
DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII MfeI MslI MspA1I
NcoI NdeI NotI NsiI NspI PciI PflMI PmlI
PshAI PsiI PstI PvuII SapI ScaI SexAI SfcI
SmaI SmlI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AlfI AloI AlwNI
ApaI ApaLI AscI AsiSI AvrII BaeI BamHI BbeI
BmtI BsaBI BsiWI BsrBI BsrFI BsrGI BstBI BstEII
BstZ17I CspCI DraIII EcoICRI EcoNI EcoRV FseI FspI
FspAI HpaI KasI KpnI MluI MmeI MscI NaeI
NarI NgoMIV NheI NruI PacI PasI PfoI PmeI
PpiI PpuMI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI
SnaBI SpeI SrfI SwaI XbaI