Restriction map for
pFN21AE9244
(Linear) (Six-base) MAPSORT of: KIEE9244.seq Check: 189 from: 1 to: 4962
KIEE9244.orf
With 182 enzymes: SgfI *
October 3, 2019 17:18 ..
AccI GT'mk_AC
Cuts at: 0 3223 4962
Size: 3223 1739
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 356 383 706 1651 1943 2732 2785 3013
Size: 356 27 323 945 292 789 53 228
Cuts at: 3013 3485 3923 4856 4962
Size: 472 438 933 106
Fragments arranged by size:
945 933 789 472 438 356 323 292
228 106 53 27
AhdI GACnn_n'nnGTC
Cuts at: 0 1274 4962
Size: 1274 3688
AleI CACnn'nnGTG
Cuts at: 0 3185 4962
Size: 3185 1777
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4328 4962
Size: 4328 634
AlwNI CAG_nnn'CTG
Cuts at: 0 1026 3463 4962
Size: 1026 2437 1499
Fragments arranged by size:
2437 1499 1026
ApaLI G'TGCA_C
Cuts at: 0 2451 4962
Size: 2451 2511
ApoI r'AATT_y
Cuts at: 0 272 478 508 794 1137 1439 1880 4156
Size: 272 206 30 286 343 302 441 2276
Cuts at: 4156 4962
Size: 806
Fragments arranged by size:
2276 806 441 343 302 286 272 206
30
AseI AT'TA_AT
Cuts at: 0 1259 2969 3493 4900 4962
Size: 1259 1710 524 1407 62
Fragments arranged by size:
1710 1407 1259 524 62
BamHI G'GATC_C
Cuts at: 0 3101 4135 4962
Size: 3101 1034 827
Fragments arranged by size:
3101 1034 827
BanII G_rGCy'C
Cuts at: 0 152 3362 4962
Size: 152 3210 1600
Fragments arranged by size:
3210 1600 152
BbsI GAAGACnn'nnnn_
Cuts at: 0 344 590 1181 1598 1823 2251 2365 3002
Size: 344 246 591 417 225 428 114 637
Cuts at: 3002 3137 4962
Size: 135 1825
Fragments arranged by size:
1825 637 591 428 417 344 246 225
135 114
BclI T'GATC_A
Cuts at: 0 974 2952 4962
Size: 974 1978 2010
Fragments arranged by size:
2010 1978 974
BfrBI ATG'CAT
Cuts at: 0 4388 4392 4962
Size: 4388 4 570
Fragments arranged by size:
4388 570 4
BglII A'GATC_T
Cuts at: 0 1737 4962
Size: 1737 3225
BlpI GC'TnA_GC
Cuts at: 0 1340 3428 4962
Size: 1340 2088 1534
Fragments arranged by size:
2088 1534 1340
Bme1580I G_kGCm'C
Cuts at: 0 1305 2455 3388 4962
Size: 1305 1150 933 1574
Fragments arranged by size:
1574 1305 1150 933
BmrI ACTGGGnnnn_n'
Cuts at: 0 539 4962
Size: 539 4423
BmtI G_CTAG'C
Cuts at: 0 3851 4962
Size: 3851 1111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 136 311 775 952 4496 4962
Size: 128 8 175 464 177 3544 466
Fragments arranged by size:
3544 466 464 177 175 128 8
Bpu10I CC'TnA_GC
Cuts at: 0 203 2861 4962
Size: 203 2658 2101
Fragments arranged by size:
2658 2101 203
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2833 4962
Size: 2833 2129
BsaI GGTCTCn'nnnn_
Cuts at: 0 2682 3392 4962
Size: 2682 710 1570
Fragments arranged by size:
2682 1570 710
BsaAI yAC'GTr
Cuts at: 0 1203 2068 4766 4962
Size: 1203 865 2698 196
Fragments arranged by size:
2698 1203 865 196
BsaBI GATnn'nnATC
Cuts at: 0 428 3983 4962
Size: 428 3555 979
Fragments arranged by size:
3555 979 428
BsaWI w'CCGG_w
Cuts at: 0 4672 4962
Size: 4672 290
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 121 151 2305 2335 4962
Size: 121 30 2154 30 2627
Fragments arranged by size:
2627 2154 121 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 129 1297 4083 4962
Size: 129 1168 2786 879
Fragments arranged by size:
2786 1168 879 129
BseYI C'CCAG_C
Cuts at: 0 1659 2598 4962
Size: 1659 939 2364
Fragments arranged by size:
2364 1659 939
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2206 2229 4962
Size: 2206 23 2733
Fragments arranged by size:
2733 2206 23
BsiHKAI G_wGCw'C
Cuts at: 0 152 2455 3182 3362 4962
Size: 152 2303 727 180 1600
Fragments arranged by size:
2303 1600 727 180 152
BsmI GAATG_Cn'
Cuts at: 0 3846 4305 4962
Size: 3846 459 657
Fragments arranged by size:
3846 657 459
Bsp1286I G_dGCh'C
Cuts at: 0 152 1305 2455 3182 3362 3388 4962
Size: 152 1153 1150 727 180 26 1574
Fragments arranged by size:
1574 1153 1150 727 180 152 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 3417 4962
Size: 3417 1545
BsrDI GCAATG_nn'
Cuts at: 0 2354 4962
Size: 2354 2608
BsrFI r'CCGG_y
Cuts at: 0 2936 4962
Size: 2936 2026
BsrGI T'GTAC_A
Cuts at: 0 1581 4602 4962
Size: 1581 3021 360
Fragments arranged by size:
3021 1581 360
BstAPI GCAn_nnn'nTGC
Cuts at: 0 237 2089 4962
Size: 237 1852 2873
Fragments arranged by size:
2873 1852 237
BstBI TT'CG_AA
Cuts at: 0 653 4962
Size: 653 4309
BstXI CCAn_nnnn'nTGG
Cuts at: 0 613 3388 4770 4962
Size: 613 2775 1382 192
Fragments arranged by size:
2775 1382 613 192
BstYI r'GATC_y
Cuts at: 0 516 1737 3101 4135 4962
Size: 516 1221 1364 1034 827
Fragments arranged by size:
1364 1221 1034 827 516
Bsu36I CC'TnA_GG
Cuts at: 0 4335 4962
Size: 4335 627
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2607 4962
Size: 2607 2355
BtsI GCAGTG_nn'
Cuts at: 0 24 1303 3612 3925 4569 4962
Size: 24 1279 2309 313 644 393
Fragments arranged by size:
2309 1279 644 393 313 24
DraI TTT'AAA
Cuts at: 0 4430 4962
Size: 4430 532
DraIII CAC_nnn'GTG
Cuts at: 0 1749 4962
Size: 1749 3213
EaeI y'GGCC_r
Cuts at: 0 3353 3390 4962
Size: 3353 37 1572
Fragments arranged by size:
3353 1572 37
EarI CTCTTCn'nnn_
Cuts at: 0 44 56 1106 1598 2435 2725 2777 2804
Size: 44 12 1050 492 837 290 52 27
Cuts at: 2804 3461 4962
Size: 657 1501
Fragments arranged by size:
1501 1050 837 657 492 290 52 44
27 12
EcoICRI GAG'CTC
Cuts at: 0 150 3360 4962
Size: 150 3210 1602
Fragments arranged by size:
3210 1602 150
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 136 311 356 383 706 775 952
Size: 128 8 175 45 27 323 69 177
Cuts at: 952 1651 1943 2732 2785 3013 3485 3923 4496
Size: 699 292 789 53 228 472 438 573
Cuts at: 4496 4856 4962
Size: 360 106
Fragments arranged by size:
789 699 573 472 438 360 323 292
228 177 175 128 106 69 53 45
27 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 365 4232 4962
Size: 365 3867 730
Fragments arranged by size:
3867 730 365
EcoO109I rG'GnC_Cy
Cuts at: 0 541 4530 4962
Size: 541 3989 432
Fragments arranged by size:
3989 541 432
EcoRI G'AATT_C
Cuts at: 0 4156 4962
Size: 4156 806
EcoRV GAT'ATC
Cuts at: 0 802 2696 4962
Size: 802 1894 2266
Fragments arranged by size:
2266 1894 802
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2379 3319 4962
Size: 2379 940 1643
Fragments arranged by size:
2379 1643 940
FspI TGC'GCA
Cuts at: 0 3766 4962
Size: 3766 1196
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 121 124 153 156 1168 1200 1260 1292
Size: 121 3 29 3 1012 32 60 32
Cuts at: 1292 2305 2337 2781 2813 4962
Size: 1013 32 444 32 2149
Fragments arranged by size:
2149 1013 1012 444 121 60 32 32
32 32 29 3 3
HincII GTy'rAC
Cuts at: 0 1268 1551 3224 4962
Size: 1268 283 1673 1738
Fragments arranged by size:
1738 1673 1268 283
HindIII A'AGCT_T
Cuts at: 0 1011 4962
Size: 1011 3951
MfeI C'AATT_G
Cuts at: 0 208 4962
Size: 208 4754
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2135 3129 3813 4962
Size: 2135 994 684 1149
Fragments arranged by size:
2135 1149 994 684
MscI TGG'CCA
Cuts at: 0 3392 4962
Size: 3392 1570
MslI CAynn'nnrTG
Cuts at: 0 1087 1481 3185 4356 4962
Size: 1087 394 1704 1171 606
Fragments arranged by size:
1704 1171 1087 606 394
MspA1I CmG'CkG
Cuts at: 0 1026 1356 4312 4962
Size: 1026 330 2956 650
Fragments arranged by size:
2956 1026 650 330
NheI G'CTAG_C
Cuts at: 0 3847 4962
Size: 3847 1115
NruI TCG'CGA
Cuts at: 0 4406 4962
Size: 4406 556
NsiI A_TGCA'T
Cuts at: 0 4390 4394 4962
Size: 4390 4 568
Fragments arranged by size:
4390 568 4
NspI r_CATG'y
Cuts at: 0 123 1381 2092 3675 4132 4392 4962
Size: 123 1258 711 1583 457 260 570
Fragments arranged by size:
1583 1258 711 570 457 260 123
PflMI CCAn_nnn'nTGG
Cuts at: 0 3607 4962
Size: 3607 1355
PfoI T'CCnGG_A
Cuts at: 0 2766 4962
Size: 2766 2196
PmlI CAC'GTG
Cuts at: 0 1203 4962
Size: 1203 3759
PpuMI rG'GwC_Cy
Cuts at: 0 541 4530 4962
Size: 541 3989 432
Fragments arranged by size:
3989 541 432
PshAI GACnn'nnGTC
Cuts at: 0 3065 4962
Size: 3065 1897
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 3137 3169 4962
Size: 3137 32 1793
Fragments arranged by size:
3137 1793 32
PstI C_TGCA'G
Cuts at: 0 169 699 1361 3711 4962
Size: 169 530 662 2350 1251
Fragments arranged by size:
2350 1251 662 530 169
PvuII CAG'CTG
Cuts at: 0 1026 1356 4312 4962
Size: 1026 330 2956 650
Fragments arranged by size:
2956 1026 650 330
SacI G_AGCT'C
Cuts at: 0 152 3362 4962
Size: 152 3210 1600
Fragments arranged by size:
3210 1600 152
SalI G'TCGA_C
Cuts at: 0 3222 4962
Size: 3222 1740
SanDI GG'GwC_CC
Cuts at: 0 541 4962
Size: 541 4421
SapI GCTCTTCn'nnn_
Cuts at: 0 1106 1598 2435 4962
Size: 1106 492 837 2527
Fragments arranged by size:
2527 1106 837 492
SfcI C'TryA_G
Cuts at: 0 165 695 1345 1357 3707 4962
Size: 165 530 650 12 2350 1255
Fragments arranged by size:
2350 1255 650 530 165 12
SmlI C'TyrA_G
Cuts at: 0 2812 4962
Size: 2812 2150
SphI G_CATG'C
Cuts at: 0 2092 4392 4962
Size: 2092 2300 570
Fragments arranged by size:
2300 2092 570
SspI AAT'ATT
Cuts at: 0 2550 3256 3772 4962
Size: 2550 706 516 1190
Fragments arranged by size:
2550 1190 706 516
StuI AGG'CCT
Cuts at: 0 659 4962
Size: 659 4303
StyI C'CwwG_G
Cuts at: 0 3393 3434 3559 3617 4201 4962
Size: 3393 41 125 58 584 761
Fragments arranged by size:
3393 761 584 125 58 41
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 356 4820 4962
Size: 356 4464 142
Fragments arranged by size:
4464 356 142
TatI w'GTAC_w
Cuts at: 0 1326 1581 1647 3144 3638 4602 4858 4962
Size: 1326 255 66 1497 494 964 256 104
Fragments arranged by size:
1497 1326 964 494 256 255 104 66
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 93 125 4962
Size: 93 32 4837
Fragments arranged by size:
4837 93 32
XbaI T'CTAG_A
Cuts at: 0 1278 2226 3057 4956 4962
Size: 1278 948 831 1899 6
Fragments arranged by size:
1899 1278 948 831 6
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2421 3400 4168 4927 4962
Size: 2421 979 768 759 35
Fragments arranged by size:
2421 979 768 759 35
XmnI GAAnn'nnTTC
Cuts at: 0 1052 3023 3626 4298 4962
Size: 1052 1971 603 672 664
Fragments arranged by size:
1971 1052 672 664 603
Enzymes that do cut:
AccI AcuI AhdI AleI AlfI AlwNI ApaLI ApoI
AseI BamHI BanII BbsI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI
Bsp1286I BspMI BsrDI BsrFI BsrGI BstAPI BstBI BstXI
BstYI Bsu36I BtgZI BtsI DraI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI
Hin4I HincII HindIII MfeI MmeI MscI MslI MspA1I
NheI NruI NsiI NspI PflMI PfoI PmlI PpuMI
PshAI PsrI PstI PvuII SacI SalI SanDI SapI
SfcI SmlI SphI SspI StuI StyI TaqII TatI
TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AloI ApaI AscI AsiSI AvaI AvrII BaeI BanI
BbeI BbvCI BcgI BciVI BglI BmgBI BplI BsaHI
BsiEI BsiWI BsmBI BspEI BspHI BsrBI BssHII BssSI
BstEII BstZ17I BtgI ClaI CspCI DrdI EagI EciI
FseI FspAI HaeII HpaI KasI KpnI MluI NaeI
NarI NcoI NdeI NgoMIV NotI PacI PasI PciI
PmeI PpiI PsiI PspOMI PspXI PvuI RsrII SacII
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SwaI Tth111I XhoI XmaI ZraI