Restriction map for
pFN21AE9210
(Linear) (Six-base) MAPSORT of: KIEE9210.seq Check: 6865 from: 1 to: 2409
KIEE9210.orf
With 182 enzymes: SgfI *
July 3, 2012 19:01 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 371 830 1466 2207 2409
Size: 371 459 636 741 202
Fragments arranged by size:
741 636 459 371 202
AccI GT'mk_AC
Cuts at: 0 329 2409
Size: 329 2080
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1087 1636 2409
Size: 1087 549 773
Fragments arranged by size:
1087 773 549
AfeI AGC'GCT
Cuts at: 0 1354 2409
Size: 1354 1055
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 676 708 2409
Size: 676 32 1701
Fragments arranged by size:
1701 676 32
AlwNI CAG_nnn'CTG
Cuts at: 0 663 813 954 1089 1245 1992 2409
Size: 663 150 141 135 156 747 417
Fragments arranged by size:
747 663 417 156 150 141 135
ApaI G_GGCC'C
Cuts at: 0 315 390 859 1108 1266 1747 2409
Size: 315 75 469 249 158 481 662
Fragments arranged by size:
662 481 469 315 249 158 75
ApaLI G'TGCA_C
Cuts at: 0 175 817 2409
Size: 175 642 1592
Fragments arranged by size:
1592 642 175
ApoI r'AATT_y
Cuts at: 0 553 2409
Size: 553 1856
AvaI C'yCGr_G
Cuts at: 0 389 2293 2409
Size: 389 1904 116
Fragments arranged by size:
1904 389 116
BanI G'GyrC_C
Cuts at: 0 1363 1692 2195 2231 2268 2409
Size: 1363 329 503 36 37 141
Fragments arranged by size:
1363 503 329 141 37 36
BanII G_rGCy'C
Cuts at: 0 113 315 390 751 859 1108 1266 1747
Size: 113 202 75 361 108 249 158 481
Cuts at: 1747 2041 2281 2384 2409
Size: 294 240 103 25
Fragments arranged by size:
481 361 294 249 240 202 158 113
108 103 75 25
BbeI G_GCGC'C
Cuts at: 0 1367 1696 2409
Size: 1367 329 713
Fragments arranged by size:
1367 713 329
BbsI GAAGACnn'nnnn_
Cuts at: 0 145 1819 2049 2104 2409
Size: 145 1674 230 55 305
Fragments arranged by size:
1674 305 230 145 55
BbvCI CC'TCA_GC
Cuts at: 0 232 1801 2409
Size: 232 1569 608
Fragments arranged by size:
1569 608 232
BglI GCCn_nnn'nGGC
Cuts at: 0 580 1564 2363 2409
Size: 580 984 799 46
Fragments arranged by size:
984 799 580 46
BlpI GC'TnA_GC
Cuts at: 0 1270 1279 2242 2409
Size: 1270 9 963 167
Fragments arranged by size:
1270 963 167 9
Bme1580I G_kGCm'C
Cuts at: 0 151 179 315 390 821 859 1108 1266
Size: 151 28 136 75 431 38 249 158
Cuts at: 1266 1303 1747 2273 2409
Size: 37 444 526 136
Fragments arranged by size:
526 444 431 249 158 151 136 136
75 38 37 28
BmgBI CAC'GTC
Cuts at: 0 915 1905 2409
Size: 915 990 504
Fragments arranged by size:
990 915 504
BmrI ACTGGGnnnn_n'
Cuts at: 0 615 2409
Size: 615 1794
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1052 2409
Size: 1052 1357
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 780 781 1009 1246 2337 2409
Size: 439 341 1 228 237 1091 72
Fragments arranged by size:
1091 439 341 237 228 72 1
Bpu10I CC'TnA_GC
Cuts at: 0 232 1801 2409
Size: 232 1569 608
Fragments arranged by size:
1569 608 232
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 2194 2409
Size: 916 1278 215
Fragments arranged by size:
1278 916 215
BsaI GGTCTCn'nnnn_
Cuts at: 0 496 1003 2409
Size: 496 507 1406
Fragments arranged by size:
1406 507 496
BsaAI yAC'GTr
Cuts at: 0 1143 2409
Size: 1143 1266
BsaHI Gr'CG_yC
Cuts at: 0 1097 1364 1478 1693 1816 1931 2054 2409
Size: 1097 267 114 215 123 115 123 355
Fragments arranged by size:
1097 355 267 215 123 123 115 114
BsaWI w'CCGG_w
Cuts at: 0 1025 2026 2409
Size: 1025 1001 383
Fragments arranged by size:
1025 1001 383
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 571 601 676 706 733 763 1123 1153
Size: 571 30 75 30 27 30 360 30
Cuts at: 1153 2409
Size: 1256
Fragments arranged by size:
1256 571 360 75 30 30 30 30
27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 355 697 898 1144 1663 1749 2409
Size: 178 177 342 201 246 519 86 660
Fragments arranged by size:
660 519 342 246 201 178 177 86
BseYI C'CCAG_C
Cuts at: 0 843 1156 1367 1819 2034 2180 2202 2409
Size: 843 313 211 452 215 146 22 207
Fragments arranged by size:
843 452 313 215 211 207 146 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 367 694 1075 1180 2409
Size: 367 327 381 105 1229
Fragments arranged by size:
1229 381 367 327 105
BsiEI CG_ry'CG
Cuts at: 0 512 2363 2409
Size: 512 1851 46
Fragments arranged by size:
1851 512 46
BsiHKAI G_wGCw'C
Cuts at: 0 179 821 1189 2409
Size: 179 642 368 1220
Fragments arranged by size:
1220 642 368 179
BsmBI CGTCTCn'nnnn_
Cuts at: 0 538 775 2409
Size: 538 237 1634
Fragments arranged by size:
1634 538 237
Bsp1286I G_dGCh'C
Cuts at: 0 113 151 179 315 390 751 821 859
Size: 113 38 28 136 75 361 70 38
Cuts at: 859 1108 1189 1266 1303 1747 2041 2273 2281
Size: 249 81 77 37 444 294 232 8
Cuts at: 2281 2384 2409
Size: 103 25
Fragments arranged by size:
444 361 294 249 232 136 113 103
81 77 75 70 38 38 37 28
25 8
BspEI T'CCGG_A
Cuts at: 0 1025 2409
Size: 1025 1384
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 371 830 935 983 1097 1466 1676 1902
Size: 371 459 105 48 114 369 210 226
Cuts at: 1902 1964 2207 2409
Size: 62 243 202
Fragments arranged by size:
459 371 369 243 226 210 202 114
105 62 48
BsrBI CCG'CTC
Cuts at: 0 1286 2246 2409
Size: 1286 960 163
Fragments arranged by size:
1286 960 163
BsrDI GCAATG_nn'
Cuts at: 0 456 2409
Size: 456 1953
BsrFI r'CCGG_y
Cuts at: 0 398 722 1545 1933 2192 2362 2409
Size: 398 324 823 388 259 170 47
Fragments arranged by size:
823 398 388 324 259 170 47
BsrGI T'GTAC_A
Cuts at: 0 20 2409
Size: 20 2389
BssHII G'CGCG_C
Cuts at: 0 1235 1248 1274 1314 2409
Size: 1235 13 26 40 1095
Fragments arranged by size:
1235 1095 40 26 13
BssSI C'ACGA_G
Cuts at: 0 832 1372 1636 2274 2409
Size: 832 540 264 638 135
Fragments arranged by size:
832 638 540 264 135
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1089 1245 1908 2409
Size: 1089 156 663 501
Fragments arranged by size:
1089 663 501 156
BstEII G'GTnAC_C
Cuts at: 0 2133 2409
Size: 2133 276
BstXI CCAn_nnnn'nTGG
Cuts at: 0 432 1164 1638 2409
Size: 432 732 474 771
Fragments arranged by size:
771 732 474 432
BstYI r'GATC_y
Cuts at: 0 356 429 2409
Size: 356 73 1980
Fragments arranged by size:
1980 356 73
Bsu36I CC'TnA_GG
Cuts at: 0 293 2409
Size: 293 2116
BtgI C'CryG_G
Cuts at: 0 575 1052 1307 2061 2409
Size: 575 477 255 754 348
Fragments arranged by size:
754 575 477 348 255
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1374 2409
Size: 1374 1035
BtsI GCAGTG_nn'
Cuts at: 0 1966 2409
Size: 1966 443
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1034 1069 2409
Size: 1034 35 1340
Fragments arranged by size:
1340 1034 35
DraIII CAC_nnn'GTG
Cuts at: 0 134 201 902 2409
Size: 134 67 701 1507
Fragments arranged by size:
1507 701 134 67
DrdI GACnn_nn'nnGTC
Cuts at: 0 469 2409
Size: 469 1940
EaeI y'GGCC_r
Cuts at: 0 2 379 917 956 1073 1508 2360 2409
Size: 2 377 538 39 117 435 852 49
Fragments arranged by size:
852 538 435 377 117 49 39 2
EagI C'GGCC_G
Cuts at: 0 2360 2409
Size: 2360 49
EarI CTCTTCn'nnn_
Cuts at: 0 943 985 1339 1456 1723 2409
Size: 943 42 354 117 267 686
Fragments arranged by size:
943 686 354 267 117 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1315 2409
Size: 1315 1094
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 780 781 1009 1087 1246 1636 2337
Size: 439 341 1 228 78 159 390 701
Cuts at: 2337 2409
Size: 72
Fragments arranged by size:
701 439 390 341 228 159 78 72
1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 322 946 2338 2409
Size: 322 624 1392 71
Fragments arranged by size:
1392 624 322 71
EcoO109I rG'GnC_Cy
Cuts at: 0 386 609 855 1105 1262 1263 1743 1744
Size: 386 223 246 250 157 1 480 1
Cuts at: 1744 1797 1940 2331 2409
Size: 53 143 391 78
Fragments arranged by size:
480 391 386 250 246 223 157 143
78 53 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1794 2409
Size: 1794 615
HaeII r_GCGC'y
Cuts at: 0 586 1356 1367 1470 1696 1838 2409
Size: 586 770 11 103 226 142 571
Fragments arranged by size:
770 586 571 226 142 103 11
HincII GTy'rAC
Cuts at: 0 330 2409
Size: 330 2079
KasI G'GCGC_C
Cuts at: 0 1363 1692 2409
Size: 1363 329 717
Fragments arranged by size:
1363 717 329
MscI TGG'CCA
Cuts at: 0 4 381 919 958 1510 2409
Size: 4 377 538 39 552 899
Fragments arranged by size:
899 552 538 377 39 4
MslI CAynn'nnrTG
Cuts at: 0 1376 2409
Size: 1376 1033
MspA1I CmG'CkG
Cuts at: 0 305 759 867 987 1581 1614 1674 1867
Size: 305 454 108 120 594 33 60 193
Cuts at: 1867 2409
Size: 542
Fragments arranged by size:
594 542 454 305 193 120 108 60
33
NaeI GCC'GGC
Cuts at: 0 400 724 1547 1935 2364 2409
Size: 400 324 823 388 429 45
Fragments arranged by size:
823 429 400 388 324 45
NarI GG'CG_CC
Cuts at: 0 1364 1693 2409
Size: 1364 329 716
Fragments arranged by size:
1364 716 329
NcoI C'CATG_G
Cuts at: 0 575 2409
Size: 575 1834
NgoMIV G'CCGG_C
Cuts at: 0 398 722 1545 1933 2362 2409
Size: 398 324 823 388 429 47
Fragments arranged by size:
823 429 398 388 324 47
NruI TCG'CGA
Cuts at: 0 1810 2409
Size: 1810 599
NspI r_CATG'y
Cuts at: 0 852 2409
Size: 852 1557
PasI CC'CwG_GG
Cuts at: 0 859 2409
Size: 859 1550
PflMI CCAn_nnn'nTGG
Cuts at: 0 134 201 813 915 1527 1605 2222 2409
Size: 134 67 612 102 612 78 617 187
Fragments arranged by size:
617 612 612 187 134 102 78 67
PfoI T'CCnGG_A
Cuts at: 0 968 2393 2409
Size: 968 1425 16
Fragments arranged by size:
1425 968 16
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 676 708 2409
Size: 676 32 1701
Fragments arranged by size:
1701 676 32
PpuMI rG'GwC_Cy
Cuts at: 0 609 2331 2409
Size: 609 1722 78
Fragments arranged by size:
1722 609 78
PshAI GACnn'nnGTC
Cuts at: 0 325 2409
Size: 325 2084
PspOMI G'GGCC_C
Cuts at: 0 311 386 855 1104 1262 1743 2409
Size: 311 75 469 249 158 481 666
Fragments arranged by size:
666 481 469 311 249 158 75
PstI C_TGCA'G
Cuts at: 0 566 704 1586 1673 1961 2144 2330 2405
Size: 566 138 882 87 288 183 186 75
Cuts at: 2405 2409
Size: 4
Fragments arranged by size:
882 566 288 186 183 138 87 75
4
PvuI CG_AT'CG
Cuts at: 0 512 2409
Size: 512 1897
PvuII CAG'CTG
Cuts at: 0 759 867 987 1614 2409
Size: 759 108 120 627 795
Fragments arranged by size:
795 759 627 120 108
SalI G'TCGA_C
Cuts at: 0 328 2409
Size: 328 2081
SapI GCTCTTCn'nnn_
Cuts at: 0 943 985 1339 1456 2409
Size: 943 42 354 117 953
Fragments arranged by size:
953 943 354 117 42
SbfI CC_TGCA'GG
Cuts at: 0 2144 2409
Size: 2144 265
SfcI C'TryA_G
Cuts at: 0 562 700 1582 1669 1957 2140 2326 2401
Size: 562 138 882 87 288 183 186 75
Cuts at: 2401 2409
Size: 8
Fragments arranged by size:
882 562 288 186 183 138 87 75
8
SfoI GGC'GCC
Cuts at: 0 1365 1694 2409
Size: 1365 329 715
Fragments arranged by size:
1365 715 329
SmaI CCC'GGG
Cuts at: 0 391 2409
Size: 391 2018
SmlI C'TyrA_G
Cuts at: 0 931 2173 2409
Size: 931 1242 236
Fragments arranged by size:
1242 931 236
SphI G_CATG'C
Cuts at: 0 852 2409
Size: 852 1557
SrfI GCCC'GGGC
Cuts at: 0 391 2409
Size: 391 2018
StuI AGG'CCT
Cuts at: 0 598 2409
Size: 598 1811
StyI C'CwwG_G
Cuts at: 0 56 575 959 1511 1559 1733 2409
Size: 56 519 384 552 48 174 676
Fragments arranged by size:
676 552 519 384 174 56 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1225 2409
Size: 1225 1184
TatI w'GTAC_w
Cuts at: 0 20 2409
Size: 20 2389
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 571 603 1168 1200 1706 1738 2409
Size: 571 32 565 32 506 32 671
Fragments arranged by size:
671 571 565 506 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 613 2124 2409
Size: 613 1511 285
Fragments arranged by size:
1511 613 285
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 966 2391 2409
Size: 966 1425 18
Fragments arranged by size:
1425 966 18
XmaI C'CCGG_G
Cuts at: 0 389 2409
Size: 389 2020
XmnI GAAnn'nnTTC
Cuts at: 0 1621 2409
Size: 1621 788
Enzymes that do cut:
AarI AccI AcuI AfeI AloI AlwNI ApaI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII
HincII KasI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NruI NspI PasI PflMI PfoI PpiI PpuMI
PshAI PspOMI PstI PvuI PvuII SalI SapI SbfI
SfcI SfoI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AleI
AlfI AscI AseI AsiSI AvrII BaeI BamHI BcgI
BciVI BclI BfrBI BglII BmtI BsaBI BsiWI BsmI
BspHI BstBI BstZ17I ClaI DraI EcoICRI EcoRI EcoRV
FseI FspI FspAI Hin4I HindIII HpaI KpnI MfeI
MluI MmeI NdeI NheI NotI NsiI PacI PciI
PmeI PmlI PsiI PspXI PsrI RsrII SacI SacII
SanDI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SspI SwaI XbaI XhoI ZraI