Restriction map for
pFN21AE6892
(Linear) (Six-base) MAPSORT of: KIEE6892.seq Check: 4623 from: 1 to: 1083
KIEE6892.orf
With 182 enzymes: SgfI *
April 28, 2014 10:22 ..
Acc65I G'GTAC_C
Cuts at: 0 911 1046 1083
Size: 911 135 37
Fragments arranged by size:
911 135 37
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 975 1083
Size: 975 108
AfeI AGC'GCT
Cuts at: 0 391 1083
Size: 391 692
AflIII A'CryG_T
Cuts at: 0 659 1083
Size: 659 424
AlwNI CAG_nnn'CTG
Cuts at: 0 72 435 636 924 1083
Size: 72 363 201 288 159
Fragments arranged by size:
363 288 201 159 72
ApaI G_GGCC'C
Cuts at: 0 826 1083
Size: 826 257
ApaLI G'TGCA_C
Cuts at: 0 322 1083
Size: 322 761
AvaI C'yCGr_G
Cuts at: 0 110 372 1083
Size: 110 262 711
Fragments arranged by size:
711 262 110
AvrII C'CTAG_G
Cuts at: 0 133 1083
Size: 133 950
BamHI G'GATC_C
Cuts at: 0 3 127 1083
Size: 3 124 956
Fragments arranged by size:
956 124 3
BanI G'GyrC_C
Cuts at: 0 38 252 911 1046 1083
Size: 38 214 659 135 37
Fragments arranged by size:
659 214 135 38 37
BanII G_rGCy'C
Cuts at: 0 141 826 895 1083
Size: 141 685 69 188
Fragments arranged by size:
685 188 141 69
BbeI G_GCGC'C
Cuts at: 0 42 1083
Size: 42 1041
BglI GCCn_nnn'nGGC
Cuts at: 0 30 384 796 1083
Size: 30 354 412 287
Fragments arranged by size:
412 354 287 30
Bme1580I G_kGCm'C
Cuts at: 0 257 326 812 826 1002 1083
Size: 257 69 486 14 176 81
Fragments arranged by size:
486 257 176 81 69 14
BmgBI CAC'GTC
Cuts at: 0 660 1083
Size: 660 423
BmrI ACTGGGnnnn_n'
Cuts at: 0 137 339 1083
Size: 137 202 744
Fragments arranged by size:
744 202 137
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 814 1083
Size: 535 279 269
Fragments arranged by size:
535 279 269
Bpu10I CC'TnA_GC
Cuts at: 0 949 1083
Size: 949 134
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 634 1083
Size: 634 449
BsaAI yAC'GTr
Cuts at: 0 876 1083
Size: 876 207
BsaHI Gr'CG_yC
Cuts at: 0 39 863 1083
Size: 39 824 220
Fragments arranged by size:
824 220 39
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 508 538 655 685 697 727 1083
Size: 508 30 117 30 12 30 356
Fragments arranged by size:
508 356 117 30 30 30 12
BseYI C'CCAG_C
Cuts at: 0 819 826 995 1083
Size: 819 7 169 88
Fragments arranged by size:
819 169 88 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 1083
Size: 35 1048
BsiEI CG_ry'CG
Cuts at: 0 288 1083
Size: 288 795
BsiHKAI G_wGCw'C
Cuts at: 0 326 1083
Size: 326 757
BsmBI CGTCTCn'nnnn_
Cuts at: 0 107 700 964 1083
Size: 107 593 264 119
Fragments arranged by size:
593 264 119 107
Bsp1286I G_dGCh'C
Cuts at: 0 141 257 326 812 826 895 1002 1083
Size: 141 116 69 486 14 69 107 81
Fragments arranged by size:
486 141 116 107 81 69 69 14
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 107 923 1083
Size: 107 816 160
Fragments arranged by size:
816 160 107
BsrBI CCG'CTC
Cuts at: 0 549 1083
Size: 549 534
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1019 1083
Size: 1019 64
BstXI CCAn_nnnn'nTGG
Cuts at: 0 903 1083
Size: 903 180
BstYI r'GATC_y
Cuts at: 0 3 127 395 1083
Size: 3 124 268 688
Fragments arranged by size:
688 268 124 3
BtgI C'CryG_G
Cuts at: 0 34 520 812 1083
Size: 34 486 292 271
Fragments arranged by size:
486 292 271 34
BtsI GCAGTG_nn'
Cuts at: 0 55 278 1083
Size: 55 223 805
Fragments arranged by size:
805 223 55
EaeI y'GGCC_r
Cuts at: 0 31 50 528 560 1083
Size: 31 19 478 32 523
Fragments arranged by size:
523 478 32 31 19
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 535 814 975 1083
Size: 535 279 161 108
Fragments arranged by size:
535 279 161 108
EcoNI CCTnn'n_nnAGG
Cuts at: 0 688 1083
Size: 688 395
EcoO109I rG'GnC_Cy
Cuts at: 0 149 823 900 1083
Size: 149 674 77 183
Fragments arranged by size:
674 183 149 77
EcoRV GAT'ATC
Cuts at: 0 336 1083
Size: 336 747
FspI TGC'GCA
Cuts at: 0 1060 1083
Size: 1060 23
HaeII r_GCGC'y
Cuts at: 0 42 393 1083
Size: 42 351 690
Fragments arranged by size:
690 351 42
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 325 357 508 540 697 729 1083
Size: 325 32 151 32 157 32 354
Fragments arranged by size:
354 325 157 151 32 32 32
KasI G'GCGC_C
Cuts at: 0 38 1083
Size: 38 1045
KpnI G_GTAC'C
Cuts at: 0 915 1050 1083
Size: 915 135 33
Fragments arranged by size:
915 135 33
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 368 512 617 1083
Size: 368 144 105 466
Fragments arranged by size:
466 368 144 105
MscI TGG'CCA
Cuts at: 0 33 1083
Size: 33 1050
MslI CAynn'nnrTG
Cuts at: 0 223 874 1083
Size: 223 651 209
Fragments arranged by size:
651 223 209
MspA1I CmG'CkG
Cuts at: 0 44 69 239 995 1083
Size: 44 25 170 756 88
Fragments arranged by size:
756 170 88 44 25
NarI GG'CG_CC
Cuts at: 0 39 1083
Size: 39 1044
NcoI C'CATG_G
Cuts at: 0 34 1083
Size: 34 1049
PasI CC'CwG_GG
Cuts at: 0 9 1083
Size: 9 1074
PflMI CCAn_nnn'nTGG
Cuts at: 0 435 1083
Size: 435 648
PmlI CAC'GTG
Cuts at: 0 876 1083
Size: 876 207
PpuMI rG'GwC_Cy
Cuts at: 0 149 900 1083
Size: 149 751 183
Fragments arranged by size:
751 183 149
PspOMI G'GGCC_C
Cuts at: 0 822 1083
Size: 822 261
PstI C_TGCA'G
Cuts at: 0 641 920 1034 1083
Size: 641 279 114 49
Fragments arranged by size:
641 279 114 49
PvuII CAG'CTG
Cuts at: 0 69 995 1083
Size: 69 926 88
Fragments arranged by size:
926 88 69
SbfI CC_TGCA'GG
Cuts at: 0 641 920 1083
Size: 641 279 163
Fragments arranged by size:
641 279 163
SfcI C'TryA_G
Cuts at: 0 637 916 1030 1083
Size: 637 279 114 53
Fragments arranged by size:
637 279 114 53
SfoI GGC'GCC
Cuts at: 0 40 1083
Size: 40 1043
SmlI C'TyrA_G
Cuts at: 0 649 1083
Size: 649 434
StuI AGG'CCT
Cuts at: 0 799 1018 1083
Size: 799 219 65
Fragments arranged by size:
799 219 65
StyI C'CwwG_G
Cuts at: 0 34 133 689 896 1083
Size: 34 99 556 207 187
Fragments arranged by size:
556 207 187 99 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1 557 583 1083
Size: 1 556 26 500
Fragments arranged by size:
556 500 26 1
Tth111I GACn'n_nGTC
Cuts at: 0 313 711 1083
Size: 313 398 372
Fragments arranged by size:
398 372 313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 32 570 1083
Size: 32 538 513
Fragments arranged by size:
538 513 32
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AlwNI ApaI ApaLI AvaI
AvrII BamHI BanI BanII BbeI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaAI BsaHI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BstAPI
BstXI BstYI BtgI BtsI EaeI Eco57MI EcoNI EcoO109I
EcoRV FspI HaeII Hin4I KasI KpnI MmeI MscI
MslI MspA1I NarI NcoI PasI PflMI PmlI PpuMI
PspOMI PstI PvuII SbfI SfcI SfoI SmlI StuI
StyI TaqII Tth111I XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AleI
AlfI AloI ApoI AscI AseI AsiSI BaeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmtI
BplI BsaI BsaBI BsaWI BseRI BsiWI BsmI BspEI
BspHI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EagI EarI EciI EcoICRI EcoRI FalI FseI FspAI
HincII HindIII HpaI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI XbaI XhoI XmaI XmnI ZraI