Restriction map for
pFN21AE6795
(Linear) (Six-base) MAPSORT of: KIEE6795.seq Check: 440 from: 1 to: 1782
KIEE6795.orf
With 182 enzymes: SgfI *
March 16, 2015 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 993 1782
Size: 993 789
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 585 1215 1394 1782
Size: 585 630 179 388
Fragments arranged by size:
630 585 388 179
AflIII A'CryG_T
Cuts at: 0 1236 1782
Size: 1236 546
AhdI GACnn_n'nnGTC
Cuts at: 0 1240 1782
Size: 1240 542
AlwNI CAG_nnn'CTG
Cuts at: 0 894 1008 1782
Size: 894 114 774
Fragments arranged by size:
894 774 114
ApaI G_GGCC'C
Cuts at: 0 322 807 1782
Size: 322 485 975
Fragments arranged by size:
975 485 322
AvaI C'yCGr_G
Cuts at: 0 1091 1782
Size: 1091 691
BamHI G'GATC_C
Cuts at: 0 305 1782
Size: 305 1477
BanI G'GyrC_C
Cuts at: 0 1260 1782
Size: 1260 522
BanII G_rGCy'C
Cuts at: 0 32 322 581 807 1514 1782
Size: 32 290 259 226 707 268
Fragments arranged by size:
707 290 268 259 226 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 868 1042 1759 1782
Size: 868 174 717 23
Fragments arranged by size:
868 717 174 23
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1428 1462 1782
Size: 1428 34 320
Fragments arranged by size:
1428 320 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 905 1782
Size: 905 877
BclI T'GATC_A
Cuts at: 0 506 662 1782
Size: 506 156 1120
Fragments arranged by size:
1120 506 156
BglI GCCn_nnn'nGGC
Cuts at: 0 1269 1567 1651 1782
Size: 1269 298 84 131
Fragments arranged by size:
1269 298 131 84
BlpI GC'TnA_GC
Cuts at: 0 338 1508 1782
Size: 338 1170 274
Fragments arranged by size:
1170 338 274
Bme1580I G_kGCm'C
Cuts at: 0 322 807 1782
Size: 322 485 975
Fragments arranged by size:
975 485 322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 1276 1592 1782
Size: 121 1155 316 190
Fragments arranged by size:
1155 316 190 121
Bpu10I CC'TnA_GC
Cuts at: 0 1528 1782
Size: 1528 254
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1381 1782
Size: 1381 401
BsaI GGTCTCn'nnnn_
Cuts at: 0 101 1782
Size: 101 1681
BsaAI yAC'GTr
Cuts at: 0 1239 1782
Size: 1239 543
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 94 124 1782
Size: 94 30 1658
Fragments arranged by size:
1658 94 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 25 1279 1321 1504 1656 1710 1782
Size: 25 1254 42 183 152 54 72
Fragments arranged by size:
1254 183 152 72 54 42 25
BseYI C'CCAG_C
Cuts at: 0 226 654 1212 1246 1782
Size: 226 428 558 34 536
Fragments arranged by size:
558 536 428 226 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1782
Size: 539 1243
BsiHKAI G_wGCw'C
Cuts at: 0 32 800 1451 1514 1529 1782
Size: 32 768 651 63 15 253
Fragments arranged by size:
768 651 253 63 32 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 532 1782
Size: 532 1250
Bsp1286I G_dGCh'C
Cuts at: 0 32 322 581 800 807 1451 1514 1529
Size: 32 290 259 219 7 644 63 15
Cuts at: 1529 1782
Size: 253
Fragments arranged by size:
644 290 259 253 219 63 32 15
7
BspHI T'CATG_A
Cuts at: 0 689 1782
Size: 689 1093
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 993 1782
Size: 993 789
BstEII G'GTnAC_C
Cuts at: 0 1137 1782
Size: 1137 645
BstXI CCAn_nnnn'nTGG
Cuts at: 0 92 165 819 1254 1300 1782
Size: 92 73 654 435 46 482
Fragments arranged by size:
654 482 435 92 73 46
BstYI r'GATC_y
Cuts at: 0 34 305 476 1782
Size: 34 271 171 1306
Fragments arranged by size:
1306 271 171 34
BtgI C'CryG_G
Cuts at: 0 961 1782
Size: 961 821
BtsI GCAGTG_nn'
Cuts at: 0 320 551 649 825 1782
Size: 320 231 98 176 957
Fragments arranged by size:
957 320 231 176 98
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 454 489 1782
Size: 454 35 1293
Fragments arranged by size:
1293 454 35
DraIII CAC_nnn'GTG
Cuts at: 0 267 782 1782
Size: 267 515 1000
Fragments arranged by size:
1000 515 267
EaeI y'GGCC_r
Cuts at: 0 773 1402 1415 1472 1782
Size: 773 629 13 57 310
Fragments arranged by size:
773 629 310 57 13
EarI CTCTTCn'nnn_
Cuts at: 0 20 1782
Size: 20 1762
EcoICRI GAG'CTC
Cuts at: 0 30 1512 1782
Size: 30 1482 270
Fragments arranged by size:
1482 270 30
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 121 585 1215 1276 1394 1592 1782
Size: 121 464 630 61 118 198 190
Fragments arranged by size:
630 464 198 190 121 118 61
EcoO109I rG'GnC_Cy
Cuts at: 0 319 1782
Size: 319 1463
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1158 1782
Size: 1158 624
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 19 51 491 523 576 608 1226 1258
Size: 19 32 440 32 53 32 618 32
Cuts at: 1258 1414 1446 1782
Size: 156 32 336
Fragments arranged by size:
618 440 336 156 53 32 32 32
32 32 19
MscI TGG'CCA
Cuts at: 0 775 1404 1782
Size: 775 629 378
Fragments arranged by size:
775 629 378
MslI CAynn'nnrTG
Cuts at: 0 568 1258 1782
Size: 568 690 524
Fragments arranged by size:
690 568 524
MspA1I CmG'CkG
Cuts at: 0 302 345 891 963 1071 1507 1782
Size: 302 43 546 72 108 436 275
Fragments arranged by size:
546 436 302 275 108 72 43
NspI r_CATG'y
Cuts at: 0 392 464 1782
Size: 392 72 1318
Fragments arranged by size:
1318 392 72
PflMI CCAn_nnn'nTGG
Cuts at: 0 782 1346 1782
Size: 782 564 436
Fragments arranged by size:
782 564 436
PfoI T'CCnGG_A
Cuts at: 0 49 164 950 1103 1782
Size: 49 115 786 153 679
Fragments arranged by size:
786 679 153 115 49
PmlI CAC'GTG
Cuts at: 0 1239 1782
Size: 1239 543
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1705 1737 1782
Size: 1705 32 45
Fragments arranged by size:
1705 45 32
PspOMI G'GGCC_C
Cuts at: 0 318 803 1782
Size: 318 485 979
Fragments arranged by size:
979 485 318
PstI C_TGCA'G
Cuts at: 0 1070 1782
Size: 1070 712
PvuII CAG'CTG
Cuts at: 0 302 345 891 1071 1782
Size: 302 43 546 180 711
Fragments arranged by size:
711 546 302 180 43
SacI G_AGCT'C
Cuts at: 0 32 1514 1782
Size: 32 1482 268
Fragments arranged by size:
1482 268 32
SacII CC_GC'GG
Cuts at: 0 964 1782
Size: 964 818
SapI GCTCTTCn'nnn_
Cuts at: 0 20 1782
Size: 20 1762
SfcI C'TryA_G
Cuts at: 0 1066 1782
Size: 1066 716
SmlI C'TyrA_G
Cuts at: 0 1396 1782
Size: 1396 386
StyI C'CwwG_G
Cuts at: 0 412 1142 1782
Size: 412 730 640
Fragments arranged by size:
730 640 412
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 384 461 781 895 1782
Size: 384 77 320 114 887
Fragments arranged by size:
887 384 320 114 77
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 306 370 1440 1782
Size: 306 64 1070 342
Fragments arranged by size:
1070 342 306 64
Enzymes that do cut:
AarI AcuI AflIII AhdI AlwNI ApaI AvaI BamHI
BanI BanII BbsI BcgI BciVI BclI BglI BlpI
Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI BstEII
BstXI BstYI BtgI BtsI CspCI DraIII EaeI EarI
EcoICRI Eco57MI EcoO109I FalI Hin4I MscI MslI MspA1I
NspI PflMI PfoI PmlI PpiI PspOMI PstI PvuII
SacI SacII SapI SfcI SmlI StyI TaqII XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BfrBI BglII BmgBI BmrI BmtI
BplI BsaBI BsaHI BsaWI BsiEI BsiWI BsmI BspEI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstZ17I Bsu36I BtgZI ClaI DraI DrdI EagI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PpuMI PshAI PsiI PspXI
PsrI PvuI RsrII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI