Restriction map for
pFN21AE6786
(Linear) (Six-base) MAPSORT of: KIEE6786.seq Check: 774 from: 1 to: 1887
KIEE6786.orf
With 182 enzymes: SgfI *
March 16, 2015 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1365 1887
Size: 1365 522
AccI GT'mk_AC
Cuts at: 0 460 1887
Size: 460 1427
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 913 1887
Size: 913 974
AflIII A'CryG_T
Cuts at: 0 1227 1887
Size: 1227 660
AhdI GACnn_n'nnGTC
Cuts at: 0 456 1887
Size: 456 1431
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 83 385 767 1702 1887
Size: 83 302 382 935 185
Fragments arranged by size:
935 382 302 185 83
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1537 1569 1887
Size: 1537 32 318
Fragments arranged by size:
1537 318 32
AlwNI CAG_nnn'CTG
Cuts at: 0 372 499 951 1102 1653 1887
Size: 372 127 452 151 551 234
Fragments arranged by size:
551 452 372 234 151 127
ApaI G_GGCC'C
Cuts at: 0 1060 1887
Size: 1060 827
ApaLI G'TGCA_C
Cuts at: 0 1245 1735 1887
Size: 1245 490 152
Fragments arranged by size:
1245 490 152
ApoI r'AATT_y
Cuts at: 0 1777 1887
Size: 1777 110
AvaI C'yCGr_G
Cuts at: 0 575 993 1006 1060 1213 1669 1887
Size: 575 418 13 54 153 456 218
Fragments arranged by size:
575 456 418 218 153 54 13
BanI G'GyrC_C
Cuts at: 0 1105 1576 1887
Size: 1105 471 311
Fragments arranged by size:
1105 471 311
BanII G_rGCy'C
Cuts at: 0 1028 1060 1075 1145 1887
Size: 1028 32 15 70 742
Fragments arranged by size:
1028 742 70 32 15
BbeI G_GCGC'C
Cuts at: 0 1109 1887
Size: 1109 778
BbsI GAAGACnn'nnnn_
Cuts at: 0 416 1650 1887
Size: 416 1234 237
Fragments arranged by size:
1234 416 237
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 556 590 1429 1463 1887
Size: 556 34 839 34 424
Fragments arranged by size:
839 556 424 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1574 1887
Size: 1574 313
BlpI GC'TnA_GC
Cuts at: 0 1159 1887
Size: 1159 728
Bme1580I G_kGCm'C
Cuts at: 0 650 1060 1249 1579 1739 1887
Size: 650 410 189 330 160 148
Fragments arranged by size:
650 410 330 189 160 148
BmgBI CAC'GTC
Cuts at: 0 165 458 1887
Size: 165 293 1429
Fragments arranged by size:
1429 293 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 151 832 928 1129 1248 1840 1887
Size: 103 48 681 96 201 119 592 47
Fragments arranged by size:
681 592 201 119 103 96 48 47
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 1887
Size: 70 1817
BsaI GGTCTCn'nnnn_
Cuts at: 0 1266 1887
Size: 1266 621
BsaBI GATnn'nnATC
Cuts at: 0 592 1887
Size: 592 1295
BsaHI Gr'CG_yC
Cuts at: 0 1106 1887
Size: 1106 781
BsaWI w'CCGG_w
Cuts at: 0 1871 1887
Size: 1871 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 872 902 1813 1843 1850 1861 1880 1887
Size: 872 30 911 30 7 11 19 7
Fragments arranged by size:
911 872 30 30 19 11 7 7
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 370 648 651 796 1887
Size: 370 278 3 145 1091
Fragments arranged by size:
1091 370 278 145 3
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 145 727 937 1399 1887
Size: 145 582 210 462 488
Fragments arranged by size:
582 488 462 210 145
BsiEI CG_ry'CG
Cuts at: 0 1127 1887
Size: 1127 760
BsiHKAI G_wGCw'C
Cuts at: 0 137 1000 1249 1739 1887
Size: 137 863 249 490 148
Fragments arranged by size:
863 490 249 148 137
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1853 1887
Size: 1853 34
Bsp1286I G_dGCh'C
Cuts at: 0 137 650 1000 1028 1060 1075 1145 1249
Size: 137 513 350 28 32 15 70 104
Cuts at: 1249 1579 1739 1887
Size: 330 160 148
Fragments arranged by size:
513 350 330 160 148 137 104 70
32 28 15
BspHI T'CATG_A
Cuts at: 0 1323 1631 1887
Size: 1323 308 256
Fragments arranged by size:
1323 308 256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1365 1887
Size: 1365 522
BsrBI CCG'CTC
Cuts at: 0 491 942 1724 1887
Size: 491 451 782 163
Fragments arranged by size:
782 491 451 163
BstXI CCAn_nnnn'nTGG
Cuts at: 0 321 1887
Size: 321 1566
BstYI r'GATC_y
Cuts at: 0 1532 1887
Size: 1532 355
BtgI C'CryG_G
Cuts at: 0 1109 1887
Size: 1109 778
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1184 1887
Size: 1184 703
DraIII CAC_nnn'GTG
Cuts at: 0 803 1887
Size: 803 1084
EaeI y'GGCC_r
Cuts at: 0 143 474 639 1112 1124 1181 1887
Size: 143 331 165 473 12 57 706
Fragments arranged by size:
706 473 331 165 143 57 12
EagI C'GGCC_G
Cuts at: 0 1124 1887
Size: 1124 763
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 727 1545 1887
Size: 727 818 342
Fragments arranged by size:
818 727 342
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 103 151 832 913 928 1129 1248 1840
Size: 103 48 681 81 15 201 119 592
Cuts at: 1840 1887
Size: 47
Fragments arranged by size:
681 592 201 119 103 81 48 47
15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1198 1887
Size: 1198 689
EcoO109I rG'GnC_Cy
Cuts at: 0 1032 1057 1206 1718 1887
Size: 1032 25 149 512 169
Fragments arranged by size:
1032 512 169 149 25
EcoRI G'AATT_C
Cuts at: 0 1777 1887
Size: 1777 110
EcoRV GAT'ATC
Cuts at: 0 1432 1887
Size: 1432 455
FspI TGC'GCA
Cuts at: 0 934 1887
Size: 934 953
HaeII r_GCGC'y
Cuts at: 0 1109 1887
Size: 1109 778
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 442 474 872 904 954 986 1524 1556
Size: 442 32 398 32 50 32 538 32
Cuts at: 1556 1861 1887
Size: 305 26
Fragments arranged by size:
538 442 398 305 50 32 32 32
32 26
HincII GTy'rAC
Cuts at: 0 248 461 1869 1887
Size: 248 213 1408 18
Fragments arranged by size:
1408 248 213 18
HpaI GTT'AAC
Cuts at: 0 248 1887
Size: 248 1639
KasI G'GCGC_C
Cuts at: 0 1105 1887
Size: 1105 782
MluI A'CGCG_T
Cuts at: 0 1227 1887
Size: 1227 660
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 629 1490 1622 1887
Size: 629 861 132 265
Fragments arranged by size:
861 629 265 132
MscI TGG'CCA
Cuts at: 0 476 641 1887
Size: 476 165 1246
Fragments arranged by size:
1246 476 165
MslI CAynn'nnrTG
Cuts at: 0 749 1630 1759 1887
Size: 749 881 129 128
Fragments arranged by size:
881 749 129 128
MspA1I CmG'CkG
Cuts at: 0 369 810 1136 1158 1166 1887
Size: 369 441 326 22 8 721
Fragments arranged by size:
721 441 369 326 22 8
NarI GG'CG_CC
Cuts at: 0 1106 1887
Size: 1106 781
NruI TCG'CGA
Cuts at: 0 8 1887
Size: 8 1879
NspI r_CATG'y
Cuts at: 0 1689 1758 1887
Size: 1689 69 129
Fragments arranged by size:
1689 129 69
PasI CC'CwG_GG
Cuts at: 0 1854 1887
Size: 1854 33
PfoI T'CCnGG_A
Cuts at: 0 944 1887
Size: 944 943
PpuMI rG'GwC_Cy
Cuts at: 0 1206 1718 1887
Size: 1206 512 169
Fragments arranged by size:
1206 512 169
PshAI GACnn'nnGTC
Cuts at: 0 1313 1887
Size: 1313 574
PspOMI G'GGCC_C
Cuts at: 0 1056 1887
Size: 1056 831
PvuII CAG'CTG
Cuts at: 0 369 810 1158 1166 1887
Size: 369 441 348 8 721
Fragments arranged by size:
721 441 369 348 8
SalI G'TCGA_C
Cuts at: 0 459 1887
Size: 459 1428
SexAI A'CCwGG_T
Cuts at: 0 11 1887
Size: 11 1876
SfcI C'TryA_G
Cuts at: 0 984 1887
Size: 984 903
SfoI GGC'GCC
Cuts at: 0 1107 1887
Size: 1107 780
SmaI CCC'GGG
Cuts at: 0 1215 1671 1887
Size: 1215 456 216
Fragments arranged by size:
1215 456 216
SmlI C'TyrA_G
Cuts at: 0 85 1887
Size: 85 1802
SphI G_CATG'C
Cuts at: 0 1689 1887
Size: 1689 198
StuI AGG'CCT
Cuts at: 0 982 1887
Size: 982 905
StyI C'CwwG_G
Cuts at: 0 221 1887
Size: 221 1666
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 551 1382 1714 1810 1858 1887
Size: 551 831 332 96 48 29
Fragments arranged by size:
831 551 332 96 48 29
TatI w'GTAC_w
Cuts at: 0 706 1887
Size: 706 1181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1813 1845 1887
Size: 1813 32 42
Fragments arranged by size:
1813 42 32
Tth111I GACn'n_nGTC
Cuts at: 0 13 1887
Size: 13 1874
XbaI T'CTAG_A
Cuts at: 0 1416 1887
Size: 1416 471
XmaI C'CCGG_G
Cuts at: 0 1213 1669 1887
Size: 1213 456 218
Fragments arranged by size:
1213 456 218
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AlfI AloI AlwNI
ApaI ApaLI ApoI AvaI BanI BanII BbeI BbsI
BcgI BglI BlpI Bme1580I BmgBI BpmI BpuEI BsaI
BsaBI BsaHI BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BsrBI BstXI BstYI BtgI
BtgZI DraIII EaeI EagI EciI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FspI HaeII Hin4I HincII HpaI KasI
MluI MmeI MscI MslI MspA1I NarI NruI NspI
PasI PfoI PpuMI PshAI PspOMI PvuII SalI SexAI
SfcI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XbaI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AscI
AseI AsiSI AvrII BaeI BamHI BbvCI BciVI BclI
BfrBI BglII BmrI BmtI BplI Bpu10I BsaAI BseYI
BsiWI BsmI BspEI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI
DraI DrdI EarI EcoICRI FalI FseI FspAI HindIII
KpnI MfeI NaeI NcoI NdeI NgoMIV NheI NotI
NsiI PacI PciI PflMI PmeI PmlI PpiI PsiI
PspXI PsrI PstI PvuI RsrII SacI SacII SanDI
SapI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XcmI XhoI XmnI ZraI