Restriction map for
pFN21AE6784
(Linear) (Six-base) MAPSORT of: KIEE6784.seq Check: 4189 from: 1 to: 1884
KIEE6784.orf
With 182 enzymes: SgfI *
March 16, 2015 11:37 ..
AccI GT'mk_AC
Cuts at: 0 635 1682 1884
Size: 635 1047 202
Fragments arranged by size:
1047 635 202
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1119 1504 1884
Size: 1119 385 380
Fragments arranged by size:
1119 385 380
AflIII A'CryG_T
Cuts at: 0 606 1884
Size: 606 1278
AlwNI CAG_nnn'CTG
Cuts at: 0 403 1884
Size: 403 1481
ApaI G_GGCC'C
Cuts at: 0 226 512 1884
Size: 226 286 1372
Fragments arranged by size:
1372 286 226
ApaLI G'TGCA_C
Cuts at: 0 590 1884
Size: 590 1294
AseI AT'TA_AT
Cuts at: 0 1043 1884
Size: 1043 841
AvaI C'yCGr_G
Cuts at: 0 78 338 495 1780 1884
Size: 78 260 157 1285 104
Fragments arranged by size:
1285 260 157 104 78
BanI G'GyrC_C
Cuts at: 0 30 321 1110 1884
Size: 30 291 789 774
Fragments arranged by size:
789 774 291 30
BanII G_rGCy'C
Cuts at: 0 128 226 512 1884
Size: 128 98 286 1372
Fragments arranged by size:
1372 286 128 98
BbeI G_GCGC'C
Cuts at: 0 34 1884
Size: 34 1850
BbvCI CC'TCA_GC
Cuts at: 0 130 1884
Size: 130 1754
BciVI GTATCCnnnnn_n'
Cuts at: 0 283 1884
Size: 283 1601
BclI T'GATC_A
Cuts at: 0 1056 1884
Size: 1056 828
BfrBI ATG'CAT
Cuts at: 0 1745 1884
Size: 1745 139
Bme1580I G_kGCm'C
Cuts at: 0 226 324 512 594 1115 1330 1884
Size: 226 98 188 82 521 215 554
Fragments arranged by size:
554 521 226 215 188 98 82
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1884
Size: 724 1160
Bpu10I CC'TnA_GC
Cuts at: 0 130 725 1170 1884
Size: 130 595 445 714
Fragments arranged by size:
714 595 445 130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 893 1884
Size: 281 612 991
Fragments arranged by size:
991 612 281
BsaAI yAC'GTr
Cuts at: 0 609 1884
Size: 609 1275
BsaBI GATnn'nnATC
Cuts at: 0 773 1884
Size: 773 1111
BsaHI Gr'CG_yC
Cuts at: 0 31 1884
Size: 31 1853
BsaWI w'CCGG_w
Cuts at: 0 1180 1884
Size: 1180 704
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 87 1884
Size: 87 1797
BseYI C'CCAG_C
Cuts at: 0 41 116 1884
Size: 41 75 1768
Fragments arranged by size:
1768 75 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 261 1612 1884
Size: 261 1351 272
Fragments arranged by size:
1351 272 261
BsiEI CG_ry'CG
Cuts at: 0 251 1884
Size: 251 1633
BsiHKAI G_wGCw'C
Cuts at: 0 455 594 1592 1884
Size: 455 139 998 292
Fragments arranged by size:
998 455 292 139
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1777 1884
Size: 1777 107
Bsp1286I G_dGCh'C
Cuts at: 0 128 226 324 455 512 594 1115 1330
Size: 128 98 98 131 57 82 521 215
Cuts at: 1330 1592 1884
Size: 262 292
Fragments arranged by size:
521 292 262 215 131 128 98 98
82 57
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 283 1884
Size: 283 1601
BsrBI CCG'CTC
Cuts at: 0 83 1884
Size: 83 1801
BsrDI GCAATG_nn'
Cuts at: 0 1745 1841 1884
Size: 1745 96 43
Fragments arranged by size:
1745 96 43
BsrFI r'CCGG_y
Cuts at: 0 250 280 1884
Size: 250 30 1604
Fragments arranged by size:
1604 250 30
BssSI C'ACGA_G
Cuts at: 0 1381 1884
Size: 1381 503
Bsu36I CC'TnA_GG
Cuts at: 0 585 1884
Size: 585 1299
BtgI C'CryG_G
Cuts at: 0 65 194 386 1884
Size: 65 129 192 1498
Fragments arranged by size:
1498 192 129 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1012 1884
Size: 1012 872
DraI TTT'AAA
Cuts at: 0 1368 1884
Size: 1368 516
EaeI y'GGCC_r
Cuts at: 0 107 248 525 627 867 1884
Size: 107 141 277 102 240 1017
Fragments arranged by size:
1017 277 240 141 107 102
EagI C'GGCC_G
Cuts at: 0 248 1884
Size: 248 1636
EarI CTCTTCn'nnn_
Cuts at: 0 1496 1884
Size: 1496 388
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 724 1119 1504 1884
Size: 724 395 385 380
Fragments arranged by size:
724 395 385 380
EcoO109I rG'GnC_Cy
Cuts at: 0 222 508 509 1884
Size: 222 286 1 1375
Fragments arranged by size:
1375 286 222 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1525 1884
Size: 1525 359
HaeII r_GCGC'y
Cuts at: 0 34 1884
Size: 34 1850
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 46 78 79 111 1041 1073 1579 1611
Size: 46 32 1 32 930 32 506 32
Cuts at: 1611 1675 1707 1884
Size: 64 32 177
Fragments arranged by size:
930 506 177 64 46 32 32 32
32 32 1
HincII GTy'rAC
Cuts at: 0 162 650 690 1884
Size: 162 488 40 1194
Fragments arranged by size:
1194 488 162 40
KasI G'GCGC_C
Cuts at: 0 30 1884
Size: 30 1854
MfeI C'AATT_G
Cuts at: 0 1277 1301 1847 1884
Size: 1277 24 546 37
Fragments arranged by size:
1277 546 37 24
MscI TGG'CCA
Cuts at: 0 527 629 869 1884
Size: 527 102 240 1015
Fragments arranged by size:
1015 527 240 102
MslI CAynn'nnrTG
Cuts at: 0 611 1884
Size: 611 1273
MspA1I CmG'CkG
Cuts at: 0 196 1884
Size: 196 1688
NaeI GCC'GGC
Cuts at: 0 252 1884
Size: 252 1632
NarI GG'CG_CC
Cuts at: 0 31 1884
Size: 31 1853
NcoI C'CATG_G
Cuts at: 0 386 1884
Size: 386 1498
NgoMIV G'CCGG_C
Cuts at: 0 250 1884
Size: 250 1634
NsiI A_TGCA'T
Cuts at: 0 1747 1884
Size: 1747 137
NspI r_CATG'y
Cuts at: 0 997 1884
Size: 997 887
PfoI T'CCnGG_A
Cuts at: 0 1512 1884
Size: 1512 372
PsiI TTA'TAA
Cuts at: 0 1616 1884
Size: 1616 268
PspOMI G'GGCC_C
Cuts at: 0 222 508 1884
Size: 222 286 1376
Fragments arranged by size:
1376 286 222
SacII CC_GC'GG
Cuts at: 0 197 1884
Size: 197 1687
SexAI A'CCwGG_T
Cuts at: 0 47 745 1884
Size: 47 698 1139
Fragments arranged by size:
1139 698 47
SfoI GGC'GCC
Cuts at: 0 32 1884
Size: 32 1852
SgrAI Cr'CCGG_yG
Cuts at: 0 280 1884
Size: 280 1604
SmlI C'TyrA_G
Cuts at: 0 296 908 1780 1884
Size: 296 612 872 104
Fragments arranged by size:
872 612 296 104
SspI AAT'ATT
Cuts at: 0 1191 1884
Size: 1191 693
StyI C'CwwG_G
Cuts at: 0 386 1216 1676 1884
Size: 386 830 460 208
Fragments arranged by size:
830 460 386 208
TatI w'GTAC_w
Cuts at: 0 1313 1884
Size: 1313 571
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 637 1884
Size: 637 1247
XhoI C'TCGA_G
Cuts at: 0 1780 1884
Size: 1780 104
XmnI GAAnn'nnTTC
Cuts at: 0 844 1425 1505 1884
Size: 844 581 80 379
Fragments arranged by size:
844 581 379 80
Enzymes that do cut:
AccI AcuI AflIII AlwNI ApaI ApaLI AseI AvaI
BanI BanII BbeI BbvCI BciVI BclI BfrBI Bme1580I
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssSI Bsu36I BtgI BtgZI DraI EaeI
EagI EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII
KasI MfeI MscI MslI MspA1I NaeI NarI NcoI
NgoMIV NsiI NspI PfoI PsiI PspOMI SacII SexAI
SfoI SgrAI SmlI SspI StyI TatI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApoI AscI AsiSI AvrII BaeI
BamHI BbsI BcgI BglI BglII BlpI BmgBI BmrI
BmtI BplI BsaI BsaXI BsiWI BsmI BspEI BspHI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstYI BstZ17I
BtsI ClaI CspCI DraIII DrdI EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HindIII HpaI KpnI
MluI MmeI NdeI NheI NotI NruI PacI PasI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfcI SfiI SgfI SmaI SnaBI
SpeI SphI SrfI StuI SwaI TaqII TstI Tth111I
XbaI XmaI ZraI