Restriction map for
pFN21AE6781
(Linear) (Six-base) MAPSORT of: KIEE6781.seq Check: 6446 from: 1 to: 1701
KIEE6781.orf
With 182 enzymes: SgfI *
March 16, 2015 11:37 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1446 1701
Size: 1446 255
AatII G_ACGT'C
Cuts at: 0 28 1701
Size: 28 1673
Acc65I G'GTAC_C
Cuts at: 0 322 1701
Size: 322 1379
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 94 266 1664 1701
Size: 34 60 172 1398 37
Fragments arranged by size:
1398 172 60 37 34
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 753 1303 1701
Size: 753 550 398
Fragments arranged by size:
753 550 398
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 266 298 1701
Size: 266 32 1403
Fragments arranged by size:
1403 266 32
AlwNI CAG_nnn'CTG
Cuts at: 0 72 165 225 1128 1536 1701
Size: 72 93 60 903 408 165
Fragments arranged by size:
903 408 165 93 72 60
AvaI C'yCGr_G
Cuts at: 0 377 1701
Size: 377 1324
AvrII C'CTAG_G
Cuts at: 0 705 1701
Size: 705 996
BanI G'GyrC_C
Cuts at: 0 322 329 602 1022 1148 1701
Size: 322 7 273 420 126 553
Fragments arranged by size:
553 420 322 273 126 7
BanII G_rGCy'C
Cuts at: 0 368 392 805 1701
Size: 368 24 413 896
Fragments arranged by size:
896 413 368 24
BbeI G_GCGC'C
Cuts at: 0 606 1701
Size: 606 1095
BbsI GAAGACnn'nnnn_
Cuts at: 0 28 1701
Size: 28 1673
BglI GCCn_nnn'nGGC
Cuts at: 0 97 463 1016 1701
Size: 97 366 553 685
Fragments arranged by size:
685 553 366 97
BglII A'GATC_T
Cuts at: 0 815 1701
Size: 815 886
Bme1580I G_kGCm'C
Cuts at: 0 399 476 1182 1701
Size: 399 77 706 519
Fragments arranged by size:
706 519 399 77
BmrI ACTGGGnnnn_n'
Cuts at: 0 286 1701
Size: 286 1415
BmtI G_CTAG'C
Cuts at: 0 493 1701
Size: 493 1208
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 232 1701
Size: 232 1469
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 247 307 406 427 655 789 1150
Size: 202 45 60 99 21 228 134 361
Cuts at: 1150 1312 1543 1561 1701
Size: 162 231 18 140
Fragments arranged by size:
361 231 228 202 162 140 134 99
60 45 21 18
Bpu10I CC'TnA_GC
Cuts at: 0 362 1701
Size: 362 1339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1216 1291 1483 1701
Size: 1216 75 192 218
Fragments arranged by size:
1216 218 192 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 1201 1701
Size: 1201 500
BsaBI GATnn'nnATC
Cuts at: 0 814 1701
Size: 814 887
BsaHI Gr'CG_yC
Cuts at: 0 9 25 603 1508 1701
Size: 9 16 578 905 193
Fragments arranged by size:
905 578 193 16 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1006 1036 1701
Size: 1006 30 665
Fragments arranged by size:
1006 665 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1600 1701
Size: 1600 101
BseYI C'CCAG_C
Cuts at: 0 434 566 578 886 1701
Size: 434 132 12 308 815
Fragments arranged by size:
815 434 308 132 12
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 664 1321 1424 1701
Size: 556 108 657 103 277
Fragments arranged by size:
657 556 277 108 103
BsiHKAI G_wGCw'C
Cuts at: 0 218 392 1307 1367 1577 1701
Size: 218 174 915 60 210 124
Fragments arranged by size:
915 218 210 174 124 60
BsmI GAATG_Cn'
Cuts at: 0 654 994 1701
Size: 654 340 707
Fragments arranged by size:
707 654 340
Bsp1286I G_dGCh'C
Cuts at: 0 218 368 392 399 476 805 1182 1307
Size: 218 150 24 7 77 329 377 125
Cuts at: 1307 1367 1577 1701
Size: 60 210 124
Fragments arranged by size:
377 329 218 210 150 125 124 77
60 24 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 917 1280 1446 1625 1701
Size: 917 363 166 179 76
Fragments arranged by size:
917 363 179 166 76
BsrBI CCG'CTC
Cuts at: 0 420 551 1701
Size: 420 131 1150
Fragments arranged by size:
1150 420 131
BsrFI r'CCGG_y
Cuts at: 0 881 1701
Size: 881 820
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1447 1536 1701
Size: 1447 89 165
Fragments arranged by size:
1447 165 89
BstEII G'GTnAC_C
Cuts at: 0 1392 1624 1701
Size: 1392 232 77
Fragments arranged by size:
1392 232 77
BstYI r'GATC_y
Cuts at: 0 815 1353 1701
Size: 815 538 348
Fragments arranged by size:
815 538 348
Bsu36I CC'TnA_GG
Cuts at: 0 611 1701
Size: 611 1090
BtgI C'CryG_G
Cuts at: 0 1238 1701
Size: 1238 463
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 989 1701
Size: 989 712
BtsI GCAGTG_nn'
Cuts at: 0 429 730 1577 1701
Size: 429 301 847 124
Fragments arranged by size:
847 429 301 124
DraI TTT'AAA
Cuts at: 0 1001 1701
Size: 1001 700
DrdI GACnn_nn'nnGTC
Cuts at: 0 237 1701
Size: 237 1464
EaeI y'GGCC_r
Cuts at: 0 455 1701
Size: 455 1246
EarI CTCTTCn'nnn_
Cuts at: 0 26 242 479 540 1701
Size: 26 216 237 61 1161
Fragments arranged by size:
1161 237 216 61 26
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 55 1701
Size: 55 1646
EcoICRI GAG'CTC
Cuts at: 0 390 1701
Size: 390 1311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 94 202 247 266 307 406 427
Size: 34 60 108 45 19 41 99 21
Cuts at: 427 655 789 1150 1312 1543 1561 1664 1701
Size: 228 134 361 162 231 18 103 37
Fragments arranged by size:
361 231 228 162 134 108 103 99
60 45 41 37 34 21 19 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 610 1596 1701
Size: 610 986 105
Fragments arranged by size:
986 610 105
EcoO109I rG'GnC_Cy
Cuts at: 0 863 1701
Size: 863 838
FspI TGC'GCA
Cuts at: 0 1276 1701
Size: 1276 425
HaeII r_GCGC'y
Cuts at: 0 117 606 1701
Size: 117 489 1095
Fragments arranged by size:
1095 489 117
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1090 1122 1701
Size: 1090 32 579
Fragments arranged by size:
1090 579 32
KasI G'GCGC_C
Cuts at: 0 602 1701
Size: 602 1099
KpnI G_GTAC'C
Cuts at: 0 326 1701
Size: 326 1375
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 551 1701
Size: 551 1150
MscI TGG'CCA
Cuts at: 0 457 1701
Size: 457 1244
MspA1I CmG'CkG
Cuts at: 0 225 570 1383 1632 1701
Size: 225 345 813 249 69
Fragments arranged by size:
813 345 249 225 69
NarI GG'CG_CC
Cuts at: 0 603 1701
Size: 603 1098
NheI G'CTAG_C
Cuts at: 0 489 1701
Size: 489 1212
NspI r_CATG'y
Cuts at: 0 748 1701
Size: 748 953
PflMI CCAn_nnn'nTGG
Cuts at: 0 165 194 225 1644 1701
Size: 165 29 31 1419 57
Fragments arranged by size:
1419 165 57 31 29
PfoI T'CCnGG_A
Cuts at: 0 284 404 805 1701
Size: 284 120 401 896
Fragments arranged by size:
896 401 284 120
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1370 1402 1701
Size: 1370 32 299
Fragments arranged by size:
1370 299 32
PpuMI rG'GwC_Cy
Cuts at: 0 863 1701
Size: 863 838
PstI C_TGCA'G
Cuts at: 0 483 1094 1124 1217 1475 1532 1572 1622
Size: 483 611 30 93 258 57 40 50
Cuts at: 1622 1701
Size: 79
Fragments arranged by size:
611 483 258 93 79 57 50 40
30
PvuII CAG'CTG
Cuts at: 0 225 570 1383 1701
Size: 225 345 813 318
Fragments arranged by size:
813 345 318 225
SacI G_AGCT'C
Cuts at: 0 392 1701
Size: 392 1309
SapI GCTCTTCn'nnn_
Cuts at: 0 26 479 1701
Size: 26 453 1222
Fragments arranged by size:
1222 453 26
SbfI CC_TGCA'GG
Cuts at: 0 1622 1701
Size: 1622 79
ScaI AGT'ACT
Cuts at: 0 1219 1701
Size: 1219 482
SfcI C'TryA_G
Cuts at: 0 479 1090 1120 1213 1471 1528 1568 1618
Size: 479 611 30 93 258 57 40 50
Cuts at: 1618 1701
Size: 83
Fragments arranged by size:
611 479 258 93 83 57 50 40
30
SfoI GGC'GCC
Cuts at: 0 604 1701
Size: 604 1097
SmlI C'TyrA_G
Cuts at: 0 1231 1306 1462 1701
Size: 1231 75 156 239
Fragments arranged by size:
1231 239 156 75
StuI AGG'CCT
Cuts at: 0 763 1701
Size: 763 938
StyI C'CwwG_G
Cuts at: 0 705 1701
Size: 705 996
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 109 1411 1701
Size: 109 1302 290
Fragments arranged by size:
1302 290 109
TatI w'GTAC_w
Cuts at: 0 1217 1701
Size: 1217 484
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1006 1038 1701
Size: 1006 32 663
Fragments arranged by size:
1006 663 32
Tth111I GACn'n_nGTC
Cuts at: 0 1507 1701
Size: 1507 194
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 586 1497 1701
Size: 586 911 204
Fragments arranged by size:
911 586 204
XmnI GAAnn'nnTTC
Cuts at: 0 652 1701
Size: 652 1049
ZraI GAC'GTC
Cuts at: 0 26 1701
Size: 26 1675
Enzymes that do cut:
AarI AatII Acc65I AcuI AlfI AloI AlwNI AvaI
AvrII BanI BanII BbeI BbsI BglI BglII Bme1580I
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspMI BsrBI BsrFI BstAPI BstEII BstYI Bsu36I BtgI
BtgZI BtsI DraI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI KpnI
MmeI MscI MspA1I NarI NheI NspI PflMI PfoI
PpiI PpuMI PstI PvuII SacI SapI SbfI ScaI
SfcI SfoI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AccI AclI AfeI AflII AflIII AgeI AhdI AleI
ApaI ApaLI ApoI AscI AseI AsiSI BaeI BamHI
BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BsaAI
BsaWI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BsrGI
BssHII BssSI BstBI BstXI BstZ17I ClaI CspCI DraIII
EagI EcoRI EcoRV FalI FseI FspAI HincII HindIII
HpaI MfeI MluI MslI NaeI NcoI NdeI NgoMIV
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacII
SalI SanDI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI XbaI XhoI XmaI