Restriction map for
pFN21AE6780
(Linear) (Six-base) MAPSORT of: KIEE6780.seq Check: 118 from: 1 to: 1701
KIEE6780.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AccI GT'mk_AC
Cuts at: 0 401 1701
Size: 401 1300
AfeI AGC'GCT
Cuts at: 0 1630 1701
Size: 1630 71
AleI CACnn'nnGTG
Cuts at: 0 361 1701
Size: 361 1340
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 733 1672 1701
Size: 733 939 29
Fragments arranged by size:
939 733 29
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 310 342 463 495 1701
Size: 310 32 121 32 1206
Fragments arranged by size:
1206 310 121 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 468 1038 1701
Size: 468 570 663
Fragments arranged by size:
663 570 468
ApoI r'AATT_y
Cuts at: 0 19 1701
Size: 19 1682
AvaI C'yCGr_G
Cuts at: 0 345 1701
Size: 345 1356
AvrII C'CTAG_G
Cuts at: 0 1383 1701
Size: 1383 318
BamHI G'GATC_C
Cuts at: 0 341 488 1701
Size: 341 147 1213
Fragments arranged by size:
1213 341 147
BanI G'GyrC_C
Cuts at: 0 442 836 1349 1701
Size: 442 394 513 352
Fragments arranged by size:
513 442 394 352
BanII G_rGCy'C
Cuts at: 0 136 1701
Size: 136 1565
BbeI G_GCGC'C
Cuts at: 0 446 1701
Size: 446 1255
BbvCI CC'TCA_GC
Cuts at: 0 512 697 1409 1701
Size: 512 185 712 292
Fragments arranged by size:
712 512 292 185
BclI T'GATC_A
Cuts at: 0 233 1701
Size: 233 1468
BglI GCCn_nnn'nGGC
Cuts at: 0 619 707 1701
Size: 619 88 994
Fragments arranged by size:
994 619 88
BglII A'GATC_T
Cuts at: 0 1275 1701
Size: 1275 426
Bme1580I G_kGCm'C
Cuts at: 0 839 862 899 1701
Size: 839 23 37 802
Fragments arranged by size:
839 802 37 23
BmgBI CAC'GTC
Cuts at: 0 195 1014 1701
Size: 195 819 687
Fragments arranged by size:
819 687 195
BmrI ACTGGGnnnn_n'
Cuts at: 0 295 1701
Size: 295 1406
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1434 1701
Size: 1434 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1529 1578 1701
Size: 490 1039 49 123
Fragments arranged by size:
1039 490 123 49
Bpu10I CC'TnA_GC
Cuts at: 0 512 697 1409 1701
Size: 512 185 712 292
Fragments arranged by size:
712 512 292 185
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 439 1590 1701
Size: 49 390 1151 111
Fragments arranged by size:
1151 390 111 49
BsaI GGTCTCn'nnnn_
Cuts at: 0 799 883 1377 1701
Size: 799 84 494 324
Fragments arranged by size:
799 494 324 84
BsaAI yAC'GTr
Cuts at: 0 393 1701
Size: 393 1308
BsaHI Gr'CG_yC
Cuts at: 0 443 1431 1701
Size: 443 988 270
Fragments arranged by size:
988 443 270
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 310 340 463 487 493 517 542 572
Size: 310 30 123 24 6 24 25 30
Cuts at: 572 1357 1387 1701
Size: 785 30 314
Fragments arranged by size:
785 314 310 123 30 30 30 25
24 24 6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 538 1701
Size: 538 1163
BseYI C'CCAG_C
Cuts at: 0 1625 1701
Size: 1625 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 1114 1252 1701
Size: 217 897 138 449
Fragments arranged by size:
897 449 217 138
BsiHKAI G_wGCw'C
Cuts at: 0 65 179 307 1418 1701
Size: 65 114 128 1111 283
Fragments arranged by size:
1111 283 128 114 65
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1438 1609 1701
Size: 1438 171 92
Fragments arranged by size:
1438 171 92
Bsp1286I G_dGCh'C
Cuts at: 0 65 136 179 307 839 862 899 1418
Size: 65 71 43 128 532 23 37 519
Cuts at: 1418 1701
Size: 283
Fragments arranged by size:
532 519 283 128 71 65 43 37
23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1277 1701
Size: 1277 424
BsrDI GCAATG_nn'
Cuts at: 0 1664 1701
Size: 1664 37
BsrFI r'CCGG_y
Cuts at: 0 386 1701
Size: 386 1315
BstAPI GCAn_nnn'nTGC
Cuts at: 0 808 1701
Size: 808 893
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1002 1500 1633 1701
Size: 1002 498 133 68
Fragments arranged by size:
1002 498 133 68
BstYI r'GATC_y
Cuts at: 0 341 465 488 1275 1448 1463 1701
Size: 341 124 23 787 173 15 238
Fragments arranged by size:
787 341 238 173 124 23 15
Bsu36I CC'TnA_GG
Cuts at: 0 461 1701
Size: 461 1240
BtsI GCAGTG_nn'
Cuts at: 0 542 897 952 1701
Size: 542 355 55 749
Fragments arranged by size:
749 542 355 55
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1209 1244 1701
Size: 1209 35 457
Fragments arranged by size:
1209 457 35
EaeI y'GGCC_r
Cuts at: 0 11 783 992 1322 1701
Size: 11 772 209 330 379
Fragments arranged by size:
772 379 330 209 11
EarI CTCTTCn'nnn_
Cuts at: 0 101 1285 1360 1701
Size: 101 1184 75 341
Fragments arranged by size:
1184 341 101 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 490 1529 1578 1701
Size: 490 1039 49 123
Fragments arranged by size:
1039 490 123 49
EcoNI CCTnn'n_nnAGG
Cuts at: 0 655 1408 1701
Size: 655 753 293
Fragments arranged by size:
753 655 293
EcoO109I rG'GnC_Cy
Cuts at: 0 714 1250 1701
Size: 714 536 451
Fragments arranged by size:
714 536 451
EcoRI G'AATT_C
Cuts at: 0 19 1701
Size: 19 1682
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 432 630 1701
Size: 432 198 1071
Fragments arranged by size:
1071 432 198
HaeII r_GCGC'y
Cuts at: 0 446 1508 1632 1701
Size: 446 1062 124 69
Fragments arranged by size:
1062 446 124 69
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 225 257 487 519 646 678 1701
Size: 225 32 230 32 127 32 1023
Fragments arranged by size:
1023 230 225 127 32 32 32
KasI G'GCGC_C
Cuts at: 0 442 1701
Size: 442 1259
MscI TGG'CCA
Cuts at: 0 13 785 994 1324 1701
Size: 13 772 209 330 377
Fragments arranged by size:
772 377 330 209 13
MslI CAynn'nnrTG
Cuts at: 0 235 361 1701
Size: 235 126 1340
Fragments arranged by size:
1340 235 126
MspA1I CmG'CkG
Cuts at: 0 1110 1701
Size: 1110 591
NaeI GCC'GGC
Cuts at: 0 388 1701
Size: 388 1313
NarI GG'CG_CC
Cuts at: 0 443 1701
Size: 443 1258
NgoMIV G'CCGG_C
Cuts at: 0 386 1701
Size: 386 1315
NspI r_CATG'y
Cuts at: 0 811 1473 1701
Size: 811 662 228
Fragments arranged by size:
811 662 228
PfoI T'CCnGG_A
Cuts at: 0 23 491 1121 1175 1701
Size: 23 468 630 54 526
Fragments arranged by size:
630 526 468 54 23
PmlI CAC'GTG
Cuts at: 0 393 1701
Size: 393 1308
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 310 342 463 495 1701
Size: 310 32 121 32 1206
Fragments arranged by size:
1206 310 121 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1250 1701
Size: 1250 451
PstI C_TGCA'G
Cuts at: 0 50 549 947 1109 1701
Size: 50 499 398 162 592
Fragments arranged by size:
592 499 398 162 50
PvuII CAG'CTG
Cuts at: 0 1110 1701
Size: 1110 591
SanDI GG'GwC_CC
Cuts at: 0 1250 1701
Size: 1250 451
SexAI A'CCwGG_T
Cuts at: 0 56 986 1701
Size: 56 930 715
Fragments arranged by size:
930 715 56
SfcI C'TryA_G
Cuts at: 0 46 367 545 943 1105 1526 1695 1701
Size: 46 321 178 398 162 421 169 6
Fragments arranged by size:
421 398 321 178 169 162 46 6
SfoI GGC'GCC
Cuts at: 0 444 1701
Size: 444 1257
SmaI CCC'GGG
Cuts at: 0 347 1701
Size: 347 1354
SmlI C'TyrA_G
Cuts at: 0 64 454 1605 1701
Size: 64 390 1151 96
Fragments arranged by size:
1151 390 96 64
SphI G_CATG'C
Cuts at: 0 811 1701
Size: 811 890
StuI AGG'CCT
Cuts at: 0 583 1055 1156 1693 1701
Size: 583 472 101 537 8
Fragments arranged by size:
583 537 472 101 8
StyI C'CwwG_G
Cuts at: 0 614 995 1383 1688 1701
Size: 614 381 388 305 13
Fragments arranged by size:
614 388 381 305 13
TatI w'GTAC_w
Cuts at: 0 110 1022 1701
Size: 110 912 679
Fragments arranged by size:
912 679 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 542 574 1701
Size: 542 32 1127
Fragments arranged by size:
1127 542 32
XmaI C'CCGG_G
Cuts at: 0 345 1701
Size: 345 1356
Enzymes that do cut:
AccI AfeI AleI AlfI AloI AlwNI ApoI AvaI
AvrII BamHI BanI BanII BbeI BbvCI BclI BglI
BglII Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspMI BsrDI BsrFI BstAPI BstXI BstYI
Bsu36I BtsI CspCI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I KasI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PfoI PmlI PpiI PpuMI
PstI PvuII SanDI SexAI SfcI SfoI SmaI SmlI
SphI StuI StyI TatI TstI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI ApaI ApaLI AscI AseI AsiSI BaeI BbsI
BcgI BciVI BfrBI BlpI BmtI BsaBI BsaWI BsiEI
BsiWI BsmI BspEI BspHI BsrBI BsrGI BssHII BssSI
BstBI BstEII BstZ17I BtgI BtgZI ClaI DraI DraIII
DrdI EagI EciI EcoICRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PciI
PflMI PmeI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI SbfI ScaI SfiI
SgfI SgrAI SnaBI SpeI SrfI SspI SwaI TaqII
Tth111I XbaI XcmI XhoI XmnI ZraI