Restriction map for
pFN21AE6778
(Linear) (Six-base) MAPSORT of: KIEE6778.seq Check: 1632 from: 1 to: 1620
KIEE6778.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 335 1188 1620
Size: 335 853 432
Fragments arranged by size:
853 432 335
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 550 1313 1620
Size: 82 468 763 307
Fragments arranged by size:
763 468 307 82
AgeI A'CCGG_T
Cuts at: 0 545 1620
Size: 545 1075
AhdI GACnn_n'nnGTC
Cuts at: 0 1326 1620
Size: 1326 294
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1462 1620
Size: 1462 158
BamHI G'GATC_C
Cuts at: 0 652 1620
Size: 652 968
BanII G_rGCy'C
Cuts at: 0 681 780 906 1016 1160 1618 1620
Size: 681 99 126 110 144 458 2
Fragments arranged by size:
681 458 144 126 110 99 2
BbsI GAAGACnn'nnnn_
Cuts at: 0 479 815 1133 1620
Size: 479 336 318 487
Fragments arranged by size:
487 479 336 318
BbvCI CC'TCA_GC
Cuts at: 0 1418 1620
Size: 1418 202
BclI T'GATC_A
Cuts at: 0 1404 1620
Size: 1404 216
BglII A'GATC_T
Cuts at: 0 666 1620
Size: 666 954
Bme1580I G_kGCm'C
Cuts at: 0 393 1620
Size: 393 1227
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 336 658 1620
Size: 336 322 962
Fragments arranged by size:
962 336 322
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 319 499 890 1620
Size: 203 116 180 391 730
Fragments arranged by size:
730 391 203 180 116
Bpu10I CC'TnA_GC
Cuts at: 0 1382 1418 1620
Size: 1382 36 202
Fragments arranged by size:
1382 202 36
BsaI GGTCTCn'nnnn_
Cuts at: 0 994 1329 1558 1620
Size: 994 335 229 62
Fragments arranged by size:
994 335 229 62
BsaAI yAC'GTr
Cuts at: 0 1035 1062 1620
Size: 1035 27 558
Fragments arranged by size:
1035 558 27
BsaWI w'CCGG_w
Cuts at: 0 545 655 1620
Size: 545 110 965
Fragments arranged by size:
965 545 110
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 201 231 1326 1356 1406 1436 1620
Size: 201 30 1095 30 50 30 184
Fragments arranged by size:
1095 201 184 50 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 206 265 316 475 1074 1327 1620
Size: 206 59 51 159 599 253 293
Fragments arranged by size:
599 293 253 206 159 59 51
BseYI C'CCAG_C
Cuts at: 0 518 1620
Size: 518 1102
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1295 1382 1620
Size: 1295 87 238
Fragments arranged by size:
1295 238 87
BsiEI CG_ry'CG
Cuts at: 0 515 1620
Size: 515 1105
BsiHKAI G_wGCw'C
Cuts at: 0 320 681 780 1016 1618 1620
Size: 320 361 99 236 602 2
Fragments arranged by size:
602 361 320 236 99 2
BsmI GAATG_Cn'
Cuts at: 0 1529 1620
Size: 1529 91
Bsp1286I G_dGCh'C
Cuts at: 0 320 393 681 780 906 1016 1160 1618
Size: 320 73 288 99 126 110 144 458
Cuts at: 1618 1620
Size: 2
Fragments arranged by size:
458 320 288 144 126 110 99 73
2
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 335 1188 1620
Size: 335 853 432
Fragments arranged by size:
853 432 335
BsrBI CCG'CTC
Cuts at: 0 270 471 1029 1317 1620
Size: 270 201 558 288 303
Fragments arranged by size:
558 303 288 270 201
BsrFI r'CCGG_y
Cuts at: 0 500 545 1620
Size: 500 45 1075
Fragments arranged by size:
1075 500 45
BstXI CCAn_nnnn'nTGG
Cuts at: 0 631 1062 1473 1620
Size: 631 431 411 147
Fragments arranged by size:
631 431 411 147
BstYI r'GATC_y
Cuts at: 0 652 666 1620
Size: 652 14 954
Fragments arranged by size:
954 652 14
BtgI C'CryG_G
Cuts at: 0 630 1346 1520 1620
Size: 630 716 174 100
Fragments arranged by size:
716 630 174 100
BtsI GCAGTG_nn'
Cuts at: 0 442 1620
Size: 442 1178
DraIII CAC_nnn'GTG
Cuts at: 0 1360 1620
Size: 1360 260
EaeI y'GGCC_r
Cuts at: 0 278 1146 1620
Size: 278 868 474
Fragments arranged by size:
868 474 278
EarI CTCTTCn'nnn_
Cuts at: 0 1289 1319 1606 1620
Size: 1289 30 287 14
Fragments arranged by size:
1289 287 30 14
EcoICRI GAG'CTC
Cuts at: 0 679 778 1014 1616 1620
Size: 679 99 236 602 4
Fragments arranged by size:
679 602 236 99 4
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 203 319 499 550 890 1313 1620
Size: 82 121 116 180 51 340 423 307
Fragments arranged by size:
423 340 307 180 121 116 82 51
EcoO109I rG'GnC_Cy
Cuts at: 0 474 1288 1459 1620
Size: 474 814 171 161
Fragments arranged by size:
814 474 171 161
EcoRV GAT'ATC
Cuts at: 0 129 1620
Size: 129 1491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 61 1620
Size: 61 1559
FspI TGC'GCA
Cuts at: 0 448 1620
Size: 448 1172
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 351 383 1064 1096 1312 1344 1357 1389
Size: 351 32 681 32 216 32 13 32
Cuts at: 1389 1620
Size: 231
Fragments arranged by size:
681 351 231 216 32 32 32 32
13
HincII GTy'rAC
Cuts at: 0 25 1620
Size: 25 1595
MfeI C'AATT_G
Cuts at: 0 1232 1620
Size: 1232 388
MspA1I CmG'CkG
Cuts at: 0 102 297 507 1552 1620
Size: 102 195 210 1045 68
Fragments arranged by size:
1045 210 195 102 68
NaeI GCC'GGC
Cuts at: 0 502 1620
Size: 502 1118
NcoI C'CATG_G
Cuts at: 0 630 1346 1620
Size: 630 716 274
Fragments arranged by size:
716 630 274
NdeI CA'TA_TG
Cuts at: 0 1539 1620
Size: 1539 81
NgoMIV G'CCGG_C
Cuts at: 0 500 1620
Size: 500 1120
PfoI T'CCnGG_A
Cuts at: 0 476 1330 1620
Size: 476 854 290
Fragments arranged by size:
854 476 290
PmlI CAC'GTG
Cuts at: 0 1035 1620
Size: 1035 585
PpuMI rG'GwC_Cy
Cuts at: 0 474 1620
Size: 474 1146
PsiI TTA'TAA
Cuts at: 0 425 754 1620
Size: 425 329 866
Fragments arranged by size:
866 425 329
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1361 1393 1620
Size: 1361 32 227
Fragments arranged by size:
1361 227 32
PstI C_TGCA'G
Cuts at: 0 197 332 353 686 1211 1620
Size: 197 135 21 333 525 409
Fragments arranged by size:
525 409 333 197 135 21
PvuII CAG'CTG
Cuts at: 0 102 297 507 1620
Size: 102 195 210 1113
Fragments arranged by size:
1113 210 195 102
SacI G_AGCT'C
Cuts at: 0 681 780 1016 1618 1620
Size: 681 99 236 602 2
Fragments arranged by size:
681 602 236 99 2
SapI GCTCTTCn'nnn_
Cuts at: 0 1606 1620
Size: 1606 14
SbfI CC_TGCA'GG
Cuts at: 0 332 1620
Size: 332 1288
SfcI C'TryA_G
Cuts at: 0 193 328 349 682 1207 1247 1620
Size: 193 135 21 333 525 40 373
Fragments arranged by size:
525 373 333 193 135 40 21
StyI C'CwwG_G
Cuts at: 0 630 736 1346 1466 1620
Size: 630 106 610 120 154
Fragments arranged by size:
630 610 154 120 106
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 637 1188 1620
Size: 637 551 432
Fragments arranged by size:
637 551 432
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 201 233 1326 1358 1406 1438 1620
Size: 201 32 1093 32 48 32 182
Fragments arranged by size:
1093 201 182 48 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1584 1620
Size: 1584 36
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 777 1620
Size: 777 843
Enzymes that do cut:
AarI AcuI AgeI AhdI AlfI BamHI BanII BbsI
BbvCI BclI BglII Bme1580I BplI BpmI Bpu10I BsaI
BsaAI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrFI BstXI BstYI BtgI
BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I EcoRV
FalI FspI Hin4I HincII MfeI MspA1I NaeI NcoI
NdeI NgoMIV PfoI PmlI PpuMI PsiI PsrI PstI
PvuII SacI SapI SbfI SfcI StyI TaqII TstI
Tth111I XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AleI
AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BcgI BciVI BfrBI
BglI BlpI BmgBI BmrI BmtI BpuEI BsaBI BsaHI
BsiWI BsmBI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DrdI EagI EciI EcoNI EcoRI FseI FspAI
HaeII HindIII HpaI KasI KpnI MluI MmeI MscI
MslI NarI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PpiI PshAI PspOMI PspXI
PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI XbaI XhoI XmaI
XmnI ZraI