Restriction map for
pFN21AE6769
(Linear) (Six-base) MAPSORT of: KIEE6769.seq Check: 3419 from: 1 to: 1602
KIEE6769.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1430 1602
Size: 1430 172
Acc65I G'GTAC_C
Cuts at: 0 1451 1602
Size: 1451 151
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 1283 1602
Size: 340 943 319
Fragments arranged by size:
943 340 319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 440 1602
Size: 440 1162
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2 34 1602
Size: 2 32 1568
Fragments arranged by size:
1568 32 2
AlwNI CAG_nnn'CTG
Cuts at: 0 119 1602
Size: 119 1483
ApoI r'AATT_y
Cuts at: 0 217 262 868 941 1602
Size: 217 45 606 73 661
Fragments arranged by size:
661 606 217 73 45
AscI GG'CGCG_CC
Cuts at: 0 1412 1602
Size: 1412 190
AseI AT'TA_AT
Cuts at: 0 884 1602
Size: 884 718
BanI G'GyrC_C
Cuts at: 0 99 416 1451 1602
Size: 99 317 1035 151
Fragments arranged by size:
1035 317 151 99
BanII G_rGCy'C
Cuts at: 0 152 1602
Size: 152 1450
BbsI GAAGACnn'nnnn_
Cuts at: 0 813 1602
Size: 813 789
BclI T'GATC_A
Cuts at: 0 410 1188 1602
Size: 410 778 414
Fragments arranged by size:
778 414 410
BfrBI ATG'CAT
Cuts at: 0 754 1602
Size: 754 848
BlpI GC'TnA_GC
Cuts at: 0 124 1602
Size: 124 1478
Bme1580I G_kGCm'C
Cuts at: 0 104 553 1602
Size: 104 449 1049
Fragments arranged by size:
1049 449 104
BmgBI CAC'GTC
Cuts at: 0 1473 1602
Size: 1473 129
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 914 1250 1317 1602
Size: 914 336 67 285
Fragments arranged by size:
914 336 285 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1602
Size: 275 1327
BsaI GGTCTCn'nnnn_
Cuts at: 0 939 1602
Size: 939 663
BsaWI w'CCGG_w
Cuts at: 0 1544 1602
Size: 1544 58
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 35 65 1217 1247 1602
Size: 35 30 1152 30 355
Fragments arranged by size:
1152 355 35 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 46 1602
Size: 43 3 1556
Fragments arranged by size:
1556 43 3
BseYI C'CCAG_C
Cuts at: 0 279 1602
Size: 279 1323
BsiHKAI G_wGCw'C
Cuts at: 0 152 1602
Size: 152 1450
BsmI GAATG_Cn'
Cuts at: 0 429 1602
Size: 429 1173
Bsp1286I G_dGCh'C
Cuts at: 0 104 152 553 1602
Size: 104 48 401 1049
Fragments arranged by size:
1049 401 104 48
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1430 1602
Size: 1430 172
BsrDI GCAATG_nn'
Cuts at: 0 1158 1602
Size: 1158 444
BssHII G'CGCG_C
Cuts at: 0 1412 1602
Size: 1412 190
BstAPI GCAn_nnn'nTGC
Cuts at: 0 700 1602
Size: 700 902
BstEII G'GTnAC_C
Cuts at: 0 1087 1602
Size: 1087 515
BstYI r'GATC_y
Cuts at: 0 738 1602
Size: 738 864
BtgI C'CryG_G
Cuts at: 0 28 247 1128 1163 1602
Size: 28 219 881 35 439
Fragments arranged by size:
881 439 219 35 28
DraI TTT'AAA
Cuts at: 0 1524 1602
Size: 1524 78
DraIII CAC_nnn'GTG
Cuts at: 0 557 1602
Size: 557 1045
EaeI y'GGCC_r
Cuts at: 0 2 35 1373 1490 1602
Size: 2 33 1338 117 112
Fragments arranged by size:
1338 117 112 33 2
EarI CTCTTCn'nnn_
Cuts at: 0 206 316 1602
Size: 206 110 1286
Fragments arranged by size:
1286 206 110
EcoICRI GAG'CTC
Cuts at: 0 150 1602
Size: 150 1452
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 914 1250 1283 1317 1602
Size: 340 574 336 33 34 285
Fragments arranged by size:
574 340 336 285 34 33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1342 1602
Size: 1342 260
EcoO109I rG'GnC_Cy
Cuts at: 0 405 1602
Size: 405 1197
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 35 67 917 949 1602
Size: 35 32 850 32 653
Fragments arranged by size:
850 653 35 32 32
HincII GTy'rAC
Cuts at: 0 462 1602
Size: 462 1140
HpaI GTT'AAC
Cuts at: 0 462 1602
Size: 462 1140
KpnI G_GTAC'C
Cuts at: 0 1455 1602
Size: 1455 147
MfeI C'AATT_G
Cuts at: 0 965 1602
Size: 965 637
MscI TGG'CCA
Cuts at: 0 4 37 1602
Size: 4 33 1565
Fragments arranged by size:
1565 33 4
MslI CAynn'nnrTG
Cuts at: 0 422 759 1168 1602
Size: 422 337 409 434
Fragments arranged by size:
434 422 409 337
MspA1I CmG'CkG
Cuts at: 0 30 1365 1602
Size: 30 1335 237
Fragments arranged by size:
1335 237 30
NcoI C'CATG_G
Cuts at: 0 247 1128 1163 1602
Size: 247 881 35 439
Fragments arranged by size:
881 439 247 35
NdeI CA'TA_TG
Cuts at: 0 1147 1602
Size: 1147 455
NsiI A_TGCA'T
Cuts at: 0 756 1602
Size: 756 846
NspI r_CATG'y
Cuts at: 0 132 642 1602
Size: 132 510 960
Fragments arranged by size:
960 510 132
PasI CC'CwG_GG
Cuts at: 0 71 1602
Size: 71 1531
PflMI CCAn_nnn'nTGG
Cuts at: 0 174 1467 1602
Size: 174 1293 135
Fragments arranged by size:
1293 174 135
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 628 660 1602
Size: 628 32 942
Fragments arranged by size:
942 628 32
SacI G_AGCT'C
Cuts at: 0 152 1602
Size: 152 1450
SacII CC_GC'GG
Cuts at: 0 31 1602
Size: 31 1571
SexAI A'CCwGG_T
Cuts at: 0 1447 1602
Size: 1447 155
SmlI C'TyrA_G
Cuts at: 0 290 1602
Size: 290 1312
SphI G_CATG'C
Cuts at: 0 132 1602
Size: 132 1470
StuI AGG'CCT
Cuts at: 0 139 1602
Size: 139 1463
StyI C'CwwG_G
Cuts at: 0 247 1128 1163 1466 1602
Size: 247 881 35 303 136
Fragments arranged by size:
881 303 247 136 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1129 1374 1602
Size: 1129 245 228
Fragments arranged by size:
1129 245 228
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 543 575 1217 1249 1602
Size: 543 32 642 32 353
Fragments arranged by size:
642 543 353 32 32
XbaI T'CTAG_A
Cuts at: 0 331 1602
Size: 331 1271
XmnI GAAnn'nnTTC
Cuts at: 0 220 314 1602
Size: 220 94 1288
Fragments arranged by size:
1288 220 94
Enzymes that do cut:
AarI Acc65I AcuI AlfI AloI AlwNI ApoI AscI
AseI BanI BanII BbsI BclI BfrBI BlpI Bme1580I
BmgBI BpmI BpuEI BsaI BsaWI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BssHII BstAPI BstEII
BstYI BtgI DraI DraIII EaeI EarI EcoICRI Eco57MI
EcoNI EcoO109I Hin4I HincII HpaI KpnI MfeI MscI
MslI MspA1I NcoI NdeI NsiI NspI PasI PflMI
PsrI SacI SacII SexAI SmlI SphI StuI StyI
TaqII TstI XbaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AleI ApaI ApaLI AsiSI AvaI AvrII BaeI BamHI
BbeI BbvCI BcgI BciVI BglI BglII BmrI BmtI
BplI Bpu10I BsaAI BsaBI BsaHI BsgI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrFI BsrGI BssSI BstBI
BstXI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DrdI
EagI EciI EcoRI EcoRV FalI FseI FspI FspAI
HaeII HindIII KasI MluI MmeI NaeI NarI NgoMIV
NheI NotI NruI PacI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SalI SanDI SapI SbfI ScaI SfcI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
SspI SwaI TatI Tth111I XcmI XhoI XmaI ZraI