Restriction map for
pFN21AE6768
(Linear) (Six-base) MAPSORT of: KIEE6768.seq Check: 8593 from: 1 to: 1596
KIEE6768.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 94 1596
Size: 94 1502
AflIII A'CryG_T
Cuts at: 0 135 722 1596
Size: 135 587 874
Fragments arranged by size:
874 587 135
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 613 645 685 717 1596
Size: 613 32 40 32 879
Fragments arranged by size:
879 613 40 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 355 1596
Size: 355 1241
ApoI r'AATT_y
Cuts at: 0 249 579 732 1596
Size: 249 330 153 864
Fragments arranged by size:
864 330 249 153
AseI AT'TA_AT
Cuts at: 0 488 1226 1596
Size: 488 738 370
Fragments arranged by size:
738 488 370
AvaI C'yCGr_G
Cuts at: 0 678 1502 1596
Size: 678 824 94
Fragments arranged by size:
824 678 94
BamHI G'GATC_C
Cuts at: 0 1365 1596
Size: 1365 231
BanII G_rGCy'C
Cuts at: 0 83 863 1129 1596
Size: 83 780 266 467
Fragments arranged by size:
780 467 266 83
BbsI GAAGACnn'nnnn_
Cuts at: 0 827 1380 1596
Size: 827 553 216
Fragments arranged by size:
827 553 216
BbvCI CC'TCA_GC
Cuts at: 0 634 1596
Size: 634 962
BciVI GTATCCnnnnn_n'
Cuts at: 0 982 1596
Size: 982 614
BclI T'GATC_A
Cuts at: 0 659 1596
Size: 659 937
BfrBI ATG'CAT
Cuts at: 0 390 1596
Size: 390 1206
BmrI ACTGGGnnnn_n'
Cuts at: 0 890 1244 1464 1596
Size: 890 354 220 132
Fragments arranged by size:
890 354 220 132
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 175 1596
Size: 175 1421
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1596
Size: 97 1499
Bpu10I CC'TnA_GC
Cuts at: 0 634 1596
Size: 634 962
BsaBI GATnn'nnATC
Cuts at: 0 1370 1596
Size: 1370 226
BsaWI w'CCGG_w
Cuts at: 0 643 1596
Size: 643 953
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 17 47 1596
Size: 17 30 1549
Fragments arranged by size:
1549 30 17
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 111 1596
Size: 111 1485
BseYI C'CCAG_C
Cuts at: 0 28 1596
Size: 28 1568
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 1596
Size: 420 1176
BsiHKAI G_wGCw'C
Cuts at: 0 863 1596
Size: 863 733
Bsp1286I G_dGCh'C
Cuts at: 0 83 863 1129 1596
Size: 83 780 266 467
Fragments arranged by size:
780 467 266 83
BspHI T'CATG_A
Cuts at: 0 704 1211 1463 1596
Size: 704 507 252 133
Fragments arranged by size:
704 507 252 133
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 94 1596
Size: 94 1502
BsrDI GCAATG_nn'
Cuts at: 0 593 1596
Size: 593 1003
BsrFI r'CCGG_y
Cuts at: 0 518 1596
Size: 518 1078
BsrGI T'GTAC_A
Cuts at: 0 1055 1509 1596
Size: 1055 454 87
Fragments arranged by size:
1055 454 87
BstXI CCAn_nnnn'nTGG
Cuts at: 0 615 1439 1596
Size: 615 824 157
Fragments arranged by size:
824 615 157
BstYI r'GATC_y
Cuts at: 0 1365 1596
Size: 1365 231
BtgI C'CryG_G
Cuts at: 0 688 1596
Size: 688 908
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 116 1596
Size: 116 1480
BtsI GCAGTG_nn'
Cuts at: 0 339 1596
Size: 339 1257
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 72 107 1141 1176 1596
Size: 72 35 1034 35 420
Fragments arranged by size:
1034 420 72 35 35
DraI TTT'AAA
Cuts at: 0 498 1596
Size: 498 1098
DraIII CAC_nnn'GTG
Cuts at: 0 1207 1596
Size: 1207 389
DrdI GACnn_nn'nnGTC
Cuts at: 0 556 973 1596
Size: 556 417 623
Fragments arranged by size:
623 556 417
EaeI y'GGCC_r
Cuts at: 0 2 366 1041 1097 1596
Size: 2 364 675 56 499
Fragments arranged by size:
675 499 364 56 2
EarI CTCTTCn'nnn_
Cuts at: 0 389 950 1596
Size: 389 561 646
Fragments arranged by size:
646 561 389
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 634 1596
Size: 634 962
EcoICRI GAG'CTC
Cuts at: 0 861 1596
Size: 861 735
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 1596
Size: 97 1499
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1083 1596
Size: 1083 513
FspI TGC'GCA
Cuts at: 0 1076 1596
Size: 1076 520
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 17 49 644 676 860 892 1507 1539
Size: 17 32 595 32 184 32 615 32
Cuts at: 1539 1596
Size: 57
Fragments arranged by size:
615 595 184 57 32 32 32 32
17
MfeI C'AATT_G
Cuts at: 0 749 1596
Size: 749 847
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 203 1596
Size: 203 1393
MscI TGG'CCA
Cuts at: 0 4 368 1043 1099 1596
Size: 4 364 675 56 497
Fragments arranged by size:
675 497 364 56 4
MslI CAynn'nnrTG
Cuts at: 0 598 1596
Size: 598 998
MspA1I CmG'CkG
Cuts at: 0 38 185 834 1596
Size: 38 147 649 762
Fragments arranged by size:
762 649 147 38
NaeI GCC'GGC
Cuts at: 0 520 1596
Size: 520 1076
NcoI C'CATG_G
Cuts at: 0 688 1596
Size: 688 908
NgoMIV G'CCGG_C
Cuts at: 0 518 1596
Size: 518 1078
NsiI A_TGCA'T
Cuts at: 0 392 1596
Size: 392 1204
NspI r_CATG'y
Cuts at: 0 139 726 1596
Size: 139 587 870
Fragments arranged by size:
870 587 139
PciI A'CATG_T
Cuts at: 0 135 722 1596
Size: 135 587 874
Fragments arranged by size:
874 587 135
PfoI T'CCnGG_A
Cuts at: 0 260 1596
Size: 260 1336
PshAI GACnn'nnGTC
Cuts at: 0 325 1596
Size: 325 1271
PspXI vC'TCGA_Gb
Cuts at: 0 1502 1596
Size: 1502 94
PstI C_TGCA'G
Cuts at: 0 334 1596
Size: 334 1262
PvuII CAG'CTG
Cuts at: 0 185 834 1596
Size: 185 649 762
Fragments arranged by size:
762 649 185
SacI G_AGCT'C
Cuts at: 0 863 1596
Size: 863 733
SapI GCTCTTCn'nnn_
Cuts at: 0 950 1596
Size: 950 646
ScaI AGT'ACT
Cuts at: 0 508 1596
Size: 508 1088
SfcI C'TryA_G
Cuts at: 0 330 1022 1139 1596
Size: 330 692 117 457
Fragments arranged by size:
692 457 330 117
SmlI C'TyrA_G
Cuts at: 0 1502 1596
Size: 1502 94
StuI AGG'CCT
Cuts at: 0 1048 1596
Size: 1048 548
StyI C'CwwG_G
Cuts at: 0 108 688 1121 1596
Size: 108 580 433 475
Fragments arranged by size:
580 475 433 108
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 645 1493 1596
Size: 645 848 103
Fragments arranged by size:
848 645 103
TatI w'GTAC_w
Cuts at: 0 506 1055 1509 1596
Size: 506 549 454 87
Fragments arranged by size:
549 506 454 87
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 755 1596
Size: 755 841
XhoI C'TCGA_G
Cuts at: 0 1502 1596
Size: 1502 94
XmnI GAAnn'nnTTC
Cuts at: 0 889 1538 1596
Size: 889 649 58
Fragments arranged by size:
889 649 58
Enzymes that do cut:
AarI AflIII AloI AlwNI ApoI AseI AvaI BamHI
BanII BbsI BbvCI BciVI BclI BfrBI BmrI BplI
BpmI Bpu10I BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BsrGI BstXI
BstYI BtgI BtgZI BtsI CspCI DraI DraIII DrdI
EaeI EarI EciI EcoICRI Eco57MI FalI FspI Hin4I
MfeI MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NsiI NspI PciI PfoI PshAI PspXI PstI PvuII
SacI SapI ScaI SfcI SmlI StuI StyI TaqII
TatI XcmI XhoI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI ApaI ApaLI AscI AsiSI AvrII
BaeI BanI BbeI BcgI BglI BglII BlpI Bme1580I
BmgBI BmtI BpuEI BsaI BsaAI BsaHI BsiEI BsiWI
BsmI BsmBI BspEI BsrBI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I ClaI EagI EcoNI EcoO109I EcoRI
EcoRV FseI FspAI HaeII HincII HindIII HpaI KasI
KpnI MluI NarI NdeI NheI NotI NruI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PsiI PspOMI
PsrI PvuI RsrII SacII SalI SanDI SbfI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XmaI ZraI