Restriction map for
pFN21AE6766
(Linear) (Six-base) MAPSORT of: KIEE6766.seq Check: 4916 from: 1 to: 1770
KIEE6766.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1569 1770
Size: 1569 201
AccI GT'mk_AC
Cuts at: 0 1019 1770
Size: 1019 751
Acc65I G'GTAC_C
Cuts at: 0 1170 1770
Size: 1170 600
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1633 1705 1770
Size: 1633 72 65
Fragments arranged by size:
1633 72 65
AflIII A'CryG_T
Cuts at: 0 306 710 1770
Size: 306 404 1060
Fragments arranged by size:
1060 404 306
AhdI GACnn_n'nnGTC
Cuts at: 0 19 1392 1770
Size: 19 1373 378
Fragments arranged by size:
1373 378 19
AleI CACnn'nnGTG
Cuts at: 0 1343 1770
Size: 1343 427
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 233 602 1770
Size: 233 369 1168
Fragments arranged by size:
1168 369 233
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 418 450 1770
Size: 418 32 1320
Fragments arranged by size:
1320 418 32
AlwNI CAG_nnn'CTG
Cuts at: 0 518 1444 1770
Size: 518 926 326
Fragments arranged by size:
926 518 326
ApaLI G'TGCA_C
Cuts at: 0 41 874 1770
Size: 41 833 896
Fragments arranged by size:
896 833 41
ApoI r'AATT_y
Cuts at: 0 1327 1770
Size: 1327 443
AvaI C'yCGr_G
Cuts at: 0 1748 1770
Size: 1748 22
BamHI G'GATC_C
Cuts at: 0 790 1373 1770
Size: 790 583 397
Fragments arranged by size:
790 583 397
BanI G'GyrC_C
Cuts at: 0 413 910 1170 1770
Size: 413 497 260 600
Fragments arranged by size:
600 497 413 260
BanII G_rGCy'C
Cuts at: 0 1574 1770
Size: 1574 196
BbsI GAAGACnn'nnnn_
Cuts at: 0 15 1770
Size: 15 1755
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 163 197 1461 1495 1770
Size: 163 34 1264 34 275
Fragments arranged by size:
1264 275 163 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 379 1770
Size: 379 1391
BglII A'GATC_T
Cuts at: 0 129 1770
Size: 129 1641
BlpI GC'TnA_GC
Cuts at: 0 622 1770
Size: 622 1148
Bme1580I G_kGCm'C
Cuts at: 0 45 752 878 1770
Size: 45 707 126 892
Fragments arranged by size:
892 707 126 45
BmgBI CAC'GTC
Cuts at: 0 307 713 1679 1770
Size: 307 406 966 91
Fragments arranged by size:
966 406 307 91
BmrI ACTGGGnnnn_n'
Cuts at: 0 735 1770
Size: 735 1035
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 182 972 1267 1755 1770
Size: 182 790 295 488 15
Fragments arranged by size:
790 488 295 182 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 1512 1770
Size: 687 825 258
Fragments arranged by size:
825 687 258
BsaI GGTCTCn'nnnn_
Cuts at: 0 6 639 1274 1770
Size: 6 633 635 496
Fragments arranged by size:
635 633 496 6
BsaAI yAC'GTr
Cuts at: 0 873 1770
Size: 873 897
BsaHI Gr'CG_yC
Cuts at: 0 163 1770
Size: 163 1607
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 418 448 1333 1363 1475 1505 1770
Size: 418 30 885 30 112 30 265
Fragments arranged by size:
885 418 265 112 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 727 1770
Size: 727 1043
BseYI C'CCAG_C
Cuts at: 0 629 1121 1770
Size: 629 492 649
Fragments arranged by size:
649 629 492
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 1342 1770
Size: 598 744 428
Fragments arranged by size:
744 598 428
BsiEI CG_ry'CG
Cuts at: 0 177 1278 1770
Size: 177 1101 492
Fragments arranged by size:
1101 492 177
BsiHKAI G_wGCw'C
Cuts at: 0 45 878 1770
Size: 45 833 892
Fragments arranged by size:
892 833 45
Bsp1286I G_dGCh'C
Cuts at: 0 45 752 878 1574 1770
Size: 45 707 126 696 196
Fragments arranged by size:
707 696 196 126 45
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1364 1569 1770
Size: 1364 205 201
Fragments arranged by size:
1364 205 201
BsrBI CCG'CTC
Cuts at: 0 626 1770
Size: 626 1144
BsrDI GCAATG_nn'
Cuts at: 0 153 590 1770
Size: 153 437 1180
Fragments arranged by size:
1180 437 153
BsrFI r'CCGG_y
Cuts at: 0 242 1770
Size: 242 1528
BssSI C'ACGA_G
Cuts at: 0 751 1770
Size: 751 1019
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1365 1770
Size: 1365 405
BstEII G'GTnAC_C
Cuts at: 0 1491 1770
Size: 1491 279
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1078 1345 1770
Size: 1078 267 425
Fragments arranged by size:
1078 425 267
BstYI r'GATC_y
Cuts at: 0 129 790 1373 1770
Size: 129 661 583 397
Fragments arranged by size:
661 583 397 129
Bsu36I CC'TnA_GG
Cuts at: 0 1000 1256 1770
Size: 1000 256 514
Fragments arranged by size:
1000 514 256
BtgI C'CryG_G
Cuts at: 0 893 959 1770
Size: 893 66 811
Fragments arranged by size:
893 811 66
ClaI AT'CG_AT
Cuts at: 0 986 1770
Size: 986 784
DraIII CAC_nnn'GTG
Cuts at: 0 72 1365 1416 1770
Size: 72 1293 51 354
Fragments arranged by size:
1293 354 72 51
EaeI y'GGCC_r
Cuts at: 0 520 1275 1601 1770
Size: 520 755 326 169
Fragments arranged by size:
755 520 326 169
EagI C'GGCC_G
Cuts at: 0 1275 1770
Size: 1275 495
EarI CTCTTCn'nnn_
Cuts at: 0 941 1522 1724 1759 1770
Size: 941 581 202 35 11
Fragments arranged by size:
941 581 202 35 11
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 1512 1633 1705 1770
Size: 687 825 121 72 65
Fragments arranged by size:
825 687 121 72 65
EcoO109I rG'GnC_Cy
Cuts at: 0 1533 1753 1770
Size: 1533 220 17
Fragments arranged by size:
1533 220 17
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 942 1770
Size: 942 828
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 5 37 257 289 296 328 1378 1410
Size: 5 32 220 32 7 32 1050 32
Cuts at: 1410 1770
Size: 360
Fragments arranged by size:
1050 360 220 32 32 32 32 7
5
HincII GTy'rAC
Cuts at: 0 510 1770
Size: 510 1260
KpnI G_GTAC'C
Cuts at: 0 1174 1770
Size: 1174 596
MscI TGG'CCA
Cuts at: 0 1603 1770
Size: 1603 167
MslI CAynn'nnrTG
Cuts at: 0 799 1343 1770
Size: 799 544 427
Fragments arranged by size:
799 544 427
MspA1I CmG'CkG
Cuts at: 0 515 1177 1770
Size: 515 662 593
Fragments arranged by size:
662 593 515
NcoI C'CATG_G
Cuts at: 0 959 1770
Size: 959 811
NspI r_CATG'y
Cuts at: 0 59 495 1069 1770
Size: 59 436 574 701
Fragments arranged by size:
701 574 436 59
PflMI CCAn_nnn'nTGG
Cuts at: 0 1444 1770
Size: 1444 326
PmlI CAC'GTG
Cuts at: 0 873 1770
Size: 873 897
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 1770
Size: 418 32 1320
Fragments arranged by size:
1320 418 32
PpuMI rG'GwC_Cy
Cuts at: 0 1533 1753 1770
Size: 1533 220 17
Fragments arranged by size:
1533 220 17
PstI C_TGCA'G
Cuts at: 0 235 1301 1770
Size: 235 1066 469
Fragments arranged by size:
1066 469 235
PvuII CAG'CTG
Cuts at: 0 515 1177 1770
Size: 515 662 593
Fragments arranged by size:
662 593 515
RsrII CG'GwC_CG
Cuts at: 0 245 1770
Size: 245 1525
SanDI GG'GwC_CC
Cuts at: 0 1533 1770
Size: 1533 237
SapI GCTCTTCn'nnn_
Cuts at: 0 1522 1770
Size: 1522 248
SfcI C'TryA_G
Cuts at: 0 231 1297 1770
Size: 231 1066 473
Fragments arranged by size:
1066 473 231
SphI G_CATG'C
Cuts at: 0 495 1069 1770
Size: 495 574 701
Fragments arranged by size:
701 574 495
SspI AAT'ATT
Cuts at: 0 93 1770
Size: 93 1677
StuI AGG'CCT
Cuts at: 0 338 1770
Size: 338 1432
StyI C'CwwG_G
Cuts at: 0 17 417 959 1379 1770
Size: 17 400 542 420 391
Fragments arranged by size:
542 420 400 391 17
Tth111I GACn'n_nGTC
Cuts at: 0 1264 1770
Size: 1264 506
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 15 1770
Size: 15 1755
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AleI AlfI
AloI AlwNI ApaLI ApoI AvaI BamHI BanI BanII
BbsI BcgI BciVI BglII BlpI Bme1580I BmgBI BmrI
BplI BpmI BsaI BsaAI BsaHI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI ClaI
DraIII EaeI EagI EarI Eco57MI EcoO109I FalI Hin4I
HincII KpnI MscI MslI MspA1I NcoI NspI PflMI
PmlI PpiI PpuMI PstI PvuII RsrII SanDI SapI
SfcI SphI SspI StuI StyI Tth111I XcmI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI ApaI AscI AseI
AsiSI AvrII BaeI BbeI BbvCI BclI BfrBI BglI
BmtI Bpu10I BpuEI BsaBI BsaWI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BssHII BstBI BstZ17I BtgZI BtsI
CspCI DraI DrdI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PfoI PmeI PshAI
PsiI PspOMI PspXI PsrI PvuI SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SwaI TaqII TatI TstI
XbaI XhoI XmaI XmnI ZraI