Restriction map for
pFN21AE6762
(Linear) (Six-base) MAPSORT of: KIEE6762.seq Check: 5244 from: 1 to: 2268
KIEE6762.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1072 2254 2268
Size: 1072 1182 14
Fragments arranged by size:
1182 1072 14
AfeI AGC'GCT
Cuts at: 0 29 472 2268
Size: 29 443 1796
Fragments arranged by size:
1796 443 29
AflIII A'CryG_T
Cuts at: 0 701 1637 2268
Size: 701 936 631
Fragments arranged by size:
936 701 631
AhdI GACnn_n'nnGTC
Cuts at: 0 1411 2268
Size: 1411 857
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1162 2268
Size: 1162 1106
AlwNI CAG_nnn'CTG
Cuts at: 0 1050 2268
Size: 1050 1218
ApaI G_GGCC'C
Cuts at: 0 194 1134 2268
Size: 194 940 1134
Fragments arranged by size:
1134 940 194
ApoI r'AATT_y
Cuts at: 0 1019 2268
Size: 1019 1249
AseI AT'TA_AT
Cuts at: 0 521 2268
Size: 521 1747
AvaI C'yCGr_G
Cuts at: 0 9 88 154 160 193 1108 1867 2268
Size: 9 79 66 6 33 915 759 401
Fragments arranged by size:
915 759 401 79 66 33 9 6
BamHI G'GATC_C
Cuts at: 0 2174 2268
Size: 2174 94
BanI G'GyrC_C
Cuts at: 0 212 599 2036 2268
Size: 212 387 1437 232
Fragments arranged by size:
1437 387 232 212
BanII G_rGCy'C
Cuts at: 0 75 134 155 161 194 227 343 1109
Size: 75 59 21 6 33 33 116 766
Cuts at: 1109 1134 1303 2268
Size: 25 169 965
Fragments arranged by size:
965 766 169 116 75 59 33 33
25 21 6
BbeI G_GCGC'C
Cuts at: 0 216 603 2040 2268
Size: 216 387 1437 228
Fragments arranged by size:
1437 387 228 216
BbsI GAAGACnn'nnnn_
Cuts at: 0 1609 2268
Size: 1609 659
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1903 1937 2268
Size: 1903 34 331
Fragments arranged by size:
1903 331 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1583 2268
Size: 1583 685
BclI T'GATC_A
Cuts at: 0 363 2268
Size: 363 1905
BglI GCCn_nnn'nGGC
Cuts at: 0 189 1046 1480 2226 2268
Size: 189 857 434 746 42
Fragments arranged by size:
857 746 434 189 42
BglII A'GATC_T
Cuts at: 0 116 1070 2268
Size: 116 954 1198
Fragments arranged by size:
1198 954 116
Bme1580I G_kGCm'C
Cuts at: 0 194 489 1134 1741 2268
Size: 194 295 645 607 527
Fragments arranged by size:
645 607 527 295 194
BmgBI CAC'GTC
Cuts at: 0 1251 2268
Size: 1251 1017
BmrI ACTGGGnnnn_n'
Cuts at: 0 669 2268
Size: 669 1599
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 186 517 2268
Size: 186 331 1751
Fragments arranged by size:
1751 331 186
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 556 749 2072 2268
Size: 141 415 193 1323 196
Fragments arranged by size:
1323 415 196 193 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 614 2268
Size: 614 1654
BsaI GGTCTCn'nnnn_
Cuts at: 0 157 1483 2268
Size: 157 1326 785
Fragments arranged by size:
1326 785 157
BsaAI yAC'GTr
Cuts at: 0 704 1638 1830 2268
Size: 704 934 192 438
Fragments arranged by size:
934 704 438 192
BsaHI Gr'CG_yC
Cuts at: 0 213 600 1691 2037 2268
Size: 213 387 1091 346 231
Fragments arranged by size:
1091 387 346 231 213
BsaWI w'CCGG_w
Cuts at: 0 285 2268
Size: 285 1983
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 156 186 2045 2075 2268
Size: 156 30 1859 30 193
Fragments arranged by size:
1859 193 156 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 1249 1713 1747 1885 2268
Size: 214 1035 464 34 138 383
Fragments arranged by size:
1035 464 383 214 138 34
BseYI C'CCAG_C
Cuts at: 0 24 1149 1308 1533 2268
Size: 24 1125 159 225 735
Fragments arranged by size:
1125 735 225 159 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1198 2268
Size: 1198 1070
BsiHKAI G_wGCw'C
Cuts at: 0 44 161 343 2268
Size: 44 117 182 1925
Fragments arranged by size:
1925 182 117 44
BsmI GAATG_Cn'
Cuts at: 0 1215 2268
Size: 1215 1053
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1231 2247 2268
Size: 1231 1016 21
Fragments arranged by size:
1231 1016 21
Bsp1286I G_dGCh'C
Cuts at: 0 44 75 134 155 161 194 227 343
Size: 44 31 59 21 6 33 33 116
Cuts at: 343 489 1109 1134 1303 1741 2268
Size: 146 620 25 169 438 527
Fragments arranged by size:
620 527 438 169 146 116 59 44
33 33 31 25 21 6
BspEI T'CCGG_A
Cuts at: 0 285 2268
Size: 285 1983
BspHI T'CATG_A
Cuts at: 0 1682 2268
Size: 1682 586
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1553 1905 2268
Size: 1553 352 363
Fragments arranged by size:
1553 363 352
BsrBI CCG'CTC
Cuts at: 0 210 2268
Size: 210 2058
BsrDI GCAATG_nn'
Cuts at: 0 696 1146 2268
Size: 696 450 1122
Fragments arranged by size:
1122 696 450
BssHII G'CGCG_C
Cuts at: 0 142 1213 2268
Size: 142 1071 1055
Fragments arranged by size:
1071 1055 142
BstBI TT'CG_AA
Cuts at: 0 1814 2268
Size: 1814 454
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1830 2268
Size: 1830 438
BstYI r'GATC_y
Cuts at: 0 116 724 764 866 888 1070 2174 2268
Size: 116 608 40 102 22 182 1104 94
Fragments arranged by size:
1104 608 182 116 102 94 40 22
BtgI C'CryG_G
Cuts at: 0 461 794 1134 1475 2268
Size: 461 333 340 341 793
Fragments arranged by size:
793 461 341 340 333
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1107 2268
Size: 1107 1161
BtsI GCAGTG_nn'
Cuts at: 0 56 583 880 2087 2268
Size: 56 527 297 1207 181
Fragments arranged by size:
1207 527 297 181 56
DraI TTT'AAA
Cuts at: 0 1376 2268
Size: 1376 892
DraIII CAC_nnn'GTG
Cuts at: 0 1230 2138 2268
Size: 1230 908 130
Fragments arranged by size:
1230 908 130
EaeI y'GGCC_r
Cuts at: 0 59 2168 2268
Size: 59 2109 100
Fragments arranged by size:
2109 100 59
EarI CTCTTCn'nnn_
Cuts at: 0 2245 2268
Size: 2245 23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 164 2262 2268
Size: 164 2098 6
Fragments arranged by size:
2098 164 6
EcoICRI GAG'CTC
Cuts at: 0 159 341 2268
Size: 159 182 1927
Fragments arranged by size:
1927 182 159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 556 749 1072 2072 2254 2268
Size: 141 415 193 323 1000 182 14
Fragments arranged by size:
1000 415 323 193 182 141 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 299 1231 2268
Size: 299 932 1037
Fragments arranged by size:
1037 932 299
EcoO109I rG'GnC_Cy
Cuts at: 0 93 190 1047 1130 1751 2268
Size: 93 97 857 83 621 517
Fragments arranged by size:
857 621 517 97 93 83
HaeII r_GCGC'y
Cuts at: 0 31 216 474 603 2040 2268
Size: 31 185 258 129 1437 228
Fragments arranged by size:
1437 258 228 185 129 31
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 156 188 491 523 1062 1094 1397 1429
Size: 156 32 303 32 539 32 303 32
Cuts at: 1429 2268
Size: 839
Fragments arranged by size:
839 539 303 303 156 32 32 32
32
KasI G'GCGC_C
Cuts at: 0 212 599 2036 2268
Size: 212 387 1437 232
Fragments arranged by size:
1437 387 232 212
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 1445 2268
Size: 48 1397 823
Fragments arranged by size:
1397 823 48
MscI TGG'CCA
Cuts at: 0 2170 2268
Size: 2170 98
MslI CAynn'nnrTG
Cuts at: 0 853 859 1139 1828 2268
Size: 853 6 280 689 440
Fragments arranged by size:
853 689 440 280 6
MspA1I CmG'CkG
Cuts at: 0 180 1646 1863 2268
Size: 180 1466 217 405
Fragments arranged by size:
1466 405 217 180
NarI GG'CG_CC
Cuts at: 0 213 600 2037 2268
Size: 213 387 1437 231
Fragments arranged by size:
1437 387 231 213
NcoI C'CATG_G
Cuts at: 0 794 2268
Size: 794 1474
PasI CC'CwG_GG
Cuts at: 0 75 1084 1323 2268
Size: 75 1009 239 945
Fragments arranged by size:
1009 945 239 75
PflMI CCAn_nnn'nTGG
Cuts at: 0 415 1230 2268
Size: 415 815 1038
Fragments arranged by size:
1038 815 415
PmlI CAC'GTG
Cuts at: 0 704 1638 2268
Size: 704 934 630
Fragments arranged by size:
934 704 630
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1096 1128 2268
Size: 1096 32 1140
Fragments arranged by size:
1140 1096 32
PpuMI rG'GwC_Cy
Cuts at: 0 93 2268
Size: 93 2175
PsiI TTA'TAA
Cuts at: 0 962 2268
Size: 962 1306
PspOMI G'GGCC_C
Cuts at: 0 190 1130 2268
Size: 190 940 1138
Fragments arranged by size:
1138 940 190
PspXI vC'TCGA_Gb
Cuts at: 0 88 2268
Size: 88 2180
PstI C_TGCA'G
Cuts at: 0 1550 2268
Size: 1550 718
PvuII CAG'CTG
Cuts at: 0 1863 2268
Size: 1863 405
SacI G_AGCT'C
Cuts at: 0 161 343 2268
Size: 161 182 1925
Fragments arranged by size:
1925 182 161
SbfI CC_TGCA'GG
Cuts at: 0 1550 2268
Size: 1550 718
ScaI AGT'ACT
Cuts at: 0 1198 2268
Size: 1198 1070
SexAI A'CCwGG_T
Cuts at: 0 300 1226 1583 2268
Size: 300 926 357 685
Fragments arranged by size:
926 685 357 300
SfcI C'TryA_G
Cuts at: 0 1546 2268
Size: 1546 722
SfoI GGC'GCC
Cuts at: 0 214 601 2038 2268
Size: 214 387 1437 230
Fragments arranged by size:
1437 387 230 214
SmaI CCC'GGG
Cuts at: 0 11 195 2268
Size: 11 184 2073
Fragments arranged by size:
2073 184 11
SmlI C'TyrA_G
Cuts at: 0 88 160 629 2268
Size: 88 72 469 1639
Fragments arranged by size:
1639 469 88 72
StuI AGG'CCT
Cuts at: 0 1288 2268
Size: 1288 980
StyI C'CwwG_G
Cuts at: 0 794 2268
Size: 794 1474
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 651 2268
Size: 651 1617
TatI w'GTAC_w
Cuts at: 0 1196 2268
Size: 1196 1072
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2045 2077 2268
Size: 2045 32 191
Fragments arranged by size:
2045 191 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 936 2106 2268
Size: 936 1170 162
Fragments arranged by size:
1170 936 162
XhoI C'TCGA_G
Cuts at: 0 88 160 2268
Size: 88 72 2108
Fragments arranged by size:
2108 88 72
XmaI C'CCGG_G
Cuts at: 0 9 193 2268
Size: 9 184 2075
Fragments arranged by size:
2075 184 9
XmnI GAAnn'nnTTC
Cuts at: 0 1786 2268
Size: 1786 482
Enzymes that do cut:
AcuI AfeI AflIII AhdI AlfI AlwNI ApaI ApoI
AseI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BssHII BstBI BstXI BstYI BtgI
BtgZI BtsI DraI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I KasI MmeI MscI
MslI MspA1I NarI NcoI PasI PflMI PmlI PpiI
PpuMI PsiI PspOMI PspXI PstI PvuII SacI SbfI
ScaI SexAI SfcI SfoI SmaI SmlI StuI StyI
TaqII TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AloI ApaLI AscI AsiSI AvrII BaeI BbvCI BfrBI
BlpI BmtI Bpu10I BsaBI BsiEI BsiWI BsrFI BsrGI
BssSI BstAPI BstEII BstZ17I Bsu36I ClaI CspCI DrdI
EagI EcoRI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PfoI
PmeI PshAI PsrI PvuI RsrII SacII SalI SanDI
SapI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I XbaI ZraI