Restriction map for
pFN21AE6760
(Linear) (Six-base) MAPSORT of: KIEE6760.seq Check: 9374 from: 1 to: 1596
KIEE6760.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 848 1069 1413 1596
Size: 508 340 221 344 183
Fragments arranged by size:
508 344 340 221 183
AflIII A'CryG_T
Cuts at: 0 1038 1158 1596
Size: 1038 120 438
Fragments arranged by size:
1038 438 120
AleI CACnn'nnGTG
Cuts at: 0 475 1596
Size: 475 1121
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 666 712 1596
Size: 666 46 884
Fragments arranged by size:
884 666 46
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1016 1048 1596
Size: 1016 32 548
Fragments arranged by size:
1016 548 32
AlwNI CAG_nnn'CTG
Cuts at: 0 865 1596
Size: 865 731
ApaLI G'TGCA_C
Cuts at: 0 801 1596
Size: 801 795
ApoI r'AATT_y
Cuts at: 0 916 1126 1596
Size: 916 210 470
Fragments arranged by size:
916 470 210
BanI G'GyrC_C
Cuts at: 0 3 499 1190 1371 1596
Size: 3 496 691 181 225
Fragments arranged by size:
691 496 225 181 3
BanII G_rGCy'C
Cuts at: 0 673 786 1596
Size: 673 113 810
Fragments arranged by size:
810 673 113
BbsI GAAGACnn'nnnn_
Cuts at: 0 836 1419 1596
Size: 836 583 177
Fragments arranged by size:
836 583 177
BclI T'GATC_A
Cuts at: 0 251 1181 1524 1596
Size: 251 930 343 72
Fragments arranged by size:
930 343 251 72
BglII A'GATC_T
Cuts at: 0 563 972 1596
Size: 563 409 624
Fragments arranged by size:
624 563 409
Bme1580I G_kGCm'C
Cuts at: 0 805 1437 1596
Size: 805 632 159
Fragments arranged by size:
805 632 159
BmgBI CAC'GTC
Cuts at: 0 240 1596
Size: 240 1356
BmrI ACTGGGnnnn_n'
Cuts at: 0 1474 1542 1596
Size: 1474 68 54
Fragments arranged by size:
1474 68 54
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 722 1596
Size: 722 874
Bpu10I CC'TnA_GC
Cuts at: 0 1376 1596
Size: 1376 220
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1016 1046 1596
Size: 1016 30 550
Fragments arranged by size:
1016 550 30
BseYI C'CCAG_C
Cuts at: 0 1574 1596
Size: 1574 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 1596
Size: 618 978
BsiEI CG_ry'CG
Cuts at: 0 127 1596
Size: 127 1469
BsiHKAI G_wGCw'C
Cuts at: 0 805 1596
Size: 805 791
BsmI GAATG_Cn'
Cuts at: 0 23 419 689 701 1479 1596
Size: 23 396 270 12 778 117
Fragments arranged by size:
778 396 270 117 23 12
Bsp1286I G_dGCh'C
Cuts at: 0 673 786 805 1437 1596
Size: 673 113 19 632 159
Fragments arranged by size:
673 632 159 113 19
BspHI T'CATG_A
Cuts at: 0 434 1596
Size: 434 1162
BsrDI GCAATG_nn'
Cuts at: 0 139 452 1091 1269 1596
Size: 139 313 639 178 327
Fragments arranged by size:
639 327 313 178 139
BsrFI r'CCGG_y
Cuts at: 0 126 1596
Size: 126 1470
BsrGI T'GTAC_A
Cuts at: 0 1208 1596
Size: 1208 388
BstBI TT'CG_AA
Cuts at: 0 693 1596
Size: 693 903
BstEII G'GTnAC_C
Cuts at: 0 1144 1596
Size: 1144 452
BstXI CCAn_nnnn'nTGG
Cuts at: 0 477 1230 1596
Size: 477 753 366
Fragments arranged by size:
753 477 366
BstYI r'GATC_y
Cuts at: 0 563 972 1596
Size: 563 409 624
Fragments arranged by size:
624 563 409
BtgI C'CryG_G
Cuts at: 0 1149 1596
Size: 1149 447
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 301 1596
Size: 301 1295
BtsI GCAGTG_nn'
Cuts at: 0 1295 1596
Size: 1295 301
DraI TTT'AAA
Cuts at: 0 905 1596
Size: 905 691
EaeI y'GGCC_r
Cuts at: 0 124 167 570 1596
Size: 124 43 403 1026
Fragments arranged by size:
1026 403 124 43
EagI C'GGCC_G
Cuts at: 0 124 1596
Size: 124 1472
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 722 848 1069 1413 1596
Size: 508 214 126 221 344 183
Fragments arranged by size:
508 344 221 214 183 126
EcoNI CCTnn'n_nnAGG
Cuts at: 0 737 1596
Size: 737 859
EcoO109I rG'GnC_Cy
Cuts at: 0 862 1596
Size: 862 734
HaeII r_GCGC'y
Cuts at: 0 49 1596
Size: 49 1547
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 854 886 928 960 1596
Size: 854 32 42 32 636
Fragments arranged by size:
854 636 42 32 32
HindIII A'AGCT_T
Cuts at: 0 316 395 1596
Size: 316 79 1201
Fragments arranged by size:
1201 316 79
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 204 1220 1596
Size: 204 1016 376
Fragments arranged by size:
1016 376 204
MscI TGG'CCA
Cuts at: 0 169 1596
Size: 169 1427
MslI CAynn'nnrTG
Cuts at: 0 475 694 1596
Size: 475 219 902
Fragments arranged by size:
902 475 219
MspA1I CmG'CkG
Cuts at: 0 165 646 1596
Size: 165 481 950
Fragments arranged by size:
950 481 165
NcoI C'CATG_G
Cuts at: 0 1149 1596
Size: 1149 447
NdeI CA'TA_TG
Cuts at: 0 588 1596
Size: 588 1008
NspI r_CATG'y
Cuts at: 0 1042 1162 1596
Size: 1042 120 434
Fragments arranged by size:
1042 434 120
PasI CC'CwG_GG
Cuts at: 0 1437 1596
Size: 1437 159
PciI A'CATG_T
Cuts at: 0 1038 1158 1596
Size: 1038 120 438
Fragments arranged by size:
1038 438 120
PflMI CCAn_nnn'nTGG
Cuts at: 0 1506 1554 1596
Size: 1506 48 42
Fragments arranged by size:
1506 48 42
PfoI T'CCnGG_A
Cuts at: 0 1584 1596
Size: 1584 12
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1016 1048 1596
Size: 1016 32 548
Fragments arranged by size:
1016 548 32
PpuMI rG'GwC_Cy
Cuts at: 0 862 1596
Size: 862 734
PvuII CAG'CTG
Cuts at: 0 165 646 1596
Size: 165 481 950
Fragments arranged by size:
950 481 165
ScaI AGT'ACT
Cuts at: 0 1063 1552 1596
Size: 1063 489 44
Fragments arranged by size:
1063 489 44
SfcI C'TryA_G
Cuts at: 0 305 525 1596
Size: 305 220 1071
Fragments arranged by size:
1071 305 220
StyI C'CwwG_G
Cuts at: 0 369 770 1149 1596
Size: 369 401 379 447
Fragments arranged by size:
447 401 379 369
TatI w'GTAC_w
Cuts at: 0 269 1061 1208 1550 1596
Size: 269 792 147 342 46
Fragments arranged by size:
792 342 269 147 46
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1507 1596
Size: 1507 89
XmnI GAAnn'nnTTC
Cuts at: 0 1016 1596
Size: 1016 580
Enzymes that do cut:
AcuI AflIII AleI AlfI AloI AlwNI ApaLI ApoI
BanI BanII BbsI BclI BglII Bme1580I BmgBI BmrI
BpmI Bpu10I BsaXI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspHI BsrDI BsrFI BsrGI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI EaeI EagI Eco57MI
EcoNI EcoO109I HaeII Hin4I HindIII MmeI MscI MslI
MspA1I NcoI NdeI NspI PasI PciI PflMI PfoI
PpiI PpuMI PvuII ScaI SfcI StyI TatI XcmI
XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI ApaI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbvCI BcgI BciVI BfrBI BglI BlpI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BseRI BsiWI BsmBI BspEI BspMI BsrBI BssHII BssSI
BstAPI BstZ17I Bsu36I ClaI CspCI DraIII DrdI EarI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HpaI KasI KpnI MfeI MluI NaeI NarI
NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI ZraI