Restriction map for
pFN21AE6758
(Linear) (Six-base) MAPSORT of: KIEE6758.seq Check: 7738 from: 1 to: 1749
KIEE6758.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AccI GT'mk_AC
Cuts at: 0 520 1256 1749
Size: 520 736 493
Fragments arranged by size:
736 520 493
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 449 1012 1092 1283 1687 1749
Size: 97 352 563 80 191 404 62
Fragments arranged by size:
563 404 352 191 97 80 62
AflIII A'CryG_T
Cuts at: 0 301 1749
Size: 301 1448
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1108 1140 1749
Size: 1108 32 609
Fragments arranged by size:
1108 609 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1503 1566 1749
Size: 1503 63 183
Fragments arranged by size:
1503 183 63
ApoI r'AATT_y
Cuts at: 0 274 564 1611 1749
Size: 274 290 1047 138
Fragments arranged by size:
1047 290 274 138
AvrII C'CTAG_G
Cuts at: 0 807 1749
Size: 807 942
BamHI G'GATC_C
Cuts at: 0 768 1749
Size: 768 981
BanI G'GyrC_C
Cuts at: 0 1374 1749
Size: 1374 375
BanII G_rGCy'C
Cuts at: 0 334 1749
Size: 334 1415
BbeI G_GCGC'C
Cuts at: 0 1378 1749
Size: 1378 371
BbsI GAAGACnn'nnnn_
Cuts at: 0 687 1749
Size: 687 1062
BbvCI CC'TCA_GC
Cuts at: 0 1370 1749
Size: 1370 379
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1452 1486 1749
Size: 1452 34 263
Fragments arranged by size:
1452 263 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1257 1749
Size: 1257 492
BlpI GC'TnA_GC
Cuts at: 0 247 1749
Size: 247 1502
BmrI ACTGGGnnnn_n'
Cuts at: 0 346 924 1440 1749
Size: 346 578 516 309
Fragments arranged by size:
578 516 346 309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 155 1135 1441 1457 1503 1749
Size: 155 980 306 16 46 246
Fragments arranged by size:
980 306 246 155 46 16
Bpu10I CC'TnA_GC
Cuts at: 0 1370 1749
Size: 1370 379
BsaHI Gr'CG_yC
Cuts at: 0 1375 1749
Size: 1375 374
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 331 361 1108 1138 1749
Size: 331 30 747 30 611
Fragments arranged by size:
747 611 331 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 67 117 471 894 937 1500 1749
Size: 67 50 354 423 43 563 249
Fragments arranged by size:
563 423 354 249 67 50 43
BseYI C'CCAG_C
Cuts at: 0 889 1749
Size: 889 860
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 482 1128 1336 1553 1749
Size: 482 646 208 217 196
Fragments arranged by size:
646 482 217 208 196
BsiHKAI G_wGCw'C
Cuts at: 0 779 816 1155 1749
Size: 779 37 339 594
Fragments arranged by size:
779 594 339 37
BsmI GAATG_Cn'
Cuts at: 0 702 745 1351 1548 1749
Size: 702 43 606 197 201
Fragments arranged by size:
702 606 201 197 43
Bsp1286I G_dGCh'C
Cuts at: 0 334 779 816 1155 1749
Size: 334 445 37 339 594
Fragments arranged by size:
594 445 339 334 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 532 1749
Size: 532 1217
BsrFI r'CCGG_y
Cuts at: 0 638 1749
Size: 638 1111
BstAPI GCAn_nnn'nTGC
Cuts at: 0 858 1749
Size: 858 891
BstEII G'GTnAC_C
Cuts at: 0 708 1749
Size: 708 1041
BstYI r'GATC_y
Cuts at: 0 422 489 768 1278 1749
Size: 422 67 279 510 471
Fragments arranged by size:
510 471 422 279 67
Bsu36I CC'TnA_GG
Cuts at: 0 335 1749
Size: 335 1414
BtgI C'CryG_G
Cuts at: 0 824 1241 1638 1749
Size: 824 417 397 111
Fragments arranged by size:
824 417 397 111
BtsI GCAGTG_nn'
Cuts at: 0 258 814 873 1591 1749
Size: 258 556 59 718 158
Fragments arranged by size:
718 556 258 158 59
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1346 1381 1749
Size: 1346 35 368
Fragments arranged by size:
1346 368 35
EaeI y'GGCC_r
Cuts at: 0 531 1334 1526 1749
Size: 531 803 192 223
Fragments arranged by size:
803 531 223 192
EarI CTCTTCn'nnn_
Cuts at: 0 116 615 1164 1259 1749
Size: 116 499 549 95 490
Fragments arranged by size:
549 499 490 116 95
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 97 155 449 1012 1092 1135 1283 1441
Size: 97 58 294 563 80 43 148 158
Cuts at: 1441 1457 1503 1687 1749
Size: 16 46 184 62
Fragments arranged by size:
563 294 184 158 148 97 80 62
58 46 43 16
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1570 1749
Size: 1570 179
EcoO109I rG'GnC_Cy
Cuts at: 0 477 1749
Size: 477 1272
FspI TGC'GCA
Cuts at: 0 1350 1749
Size: 1350 399
FspAI rTGC'GCAy
Cuts at: 0 1350 1749
Size: 1350 399
HaeII r_GCGC'y
Cuts at: 0 1378 1749
Size: 1378 371
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 31 63 331 363 474 506 1077 1109
Size: 31 32 268 32 111 32 571 32
Cuts at: 1109 1749
Size: 640
Fragments arranged by size:
640 571 268 111 32 32 32 32
31
HincII GTy'rAC
Cuts at: 0 141 1749
Size: 141 1608
KasI G'GCGC_C
Cuts at: 0 1374 1749
Size: 1374 375
MscI TGG'CCA
Cuts at: 0 533 1528 1749
Size: 533 995 221
Fragments arranged by size:
995 533 221
MslI CAynn'nnrTG
Cuts at: 0 633 760 1630 1749
Size: 633 127 870 119
Fragments arranged by size:
870 633 127 119
MspA1I CmG'CkG
Cuts at: 0 836 1503 1749
Size: 836 667 246
Fragments arranged by size:
836 667 246
NaeI GCC'GGC
Cuts at: 0 640 1749
Size: 640 1109
NarI GG'CG_CC
Cuts at: 0 1375 1749
Size: 1375 374
NcoI C'CATG_G
Cuts at: 0 824 1241 1749
Size: 824 417 508
Fragments arranged by size:
824 508 417
NgoMIV G'CCGG_C
Cuts at: 0 638 1749
Size: 638 1111
NspI r_CATG'y
Cuts at: 0 305 1349 1749
Size: 305 1044 400
Fragments arranged by size:
1044 400 305
PciI A'CATG_T
Cuts at: 0 301 1749
Size: 301 1448
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1108 1140 1749
Size: 1108 32 609
Fragments arranged by size:
1108 609 32
PpuMI rG'GwC_Cy
Cuts at: 0 477 1749
Size: 477 1272
PstI C_TGCA'G
Cuts at: 0 821 1286 1749
Size: 821 465 463
Fragments arranged by size:
821 465 463
PvuII CAG'CTG
Cuts at: 0 1503 1749
Size: 1503 246
ScaI AGT'ACT
Cuts at: 0 935 1749
Size: 935 814
SfcI C'TryA_G
Cuts at: 0 817 1282 1749
Size: 817 465 467
Fragments arranged by size:
817 467 465
SfoI GGC'GCC
Cuts at: 0 1376 1749
Size: 1376 373
SpeI A'CTAG_T
Cuts at: 0 1476 1749
Size: 1476 273
StyI C'CwwG_G
Cuts at: 0 807 824 1241 1749
Size: 807 17 417 508
Fragments arranged by size:
807 508 417 17
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 906 1749
Size: 906 843
TatI w'GTAC_w
Cuts at: 0 933 1749
Size: 933 816
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 182 214 1749
Size: 182 32 1535
Fragments arranged by size:
1535 182 32
XmnI GAAnn'nnTTC
Cuts at: 0 278 1025 1105 1749
Size: 278 747 80 644
Fragments arranged by size:
747 644 278 80
Enzymes that do cut:
AccI AcuI AflIII AloI AlwNI ApoI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BcgI BciVI BlpI
BmrI BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrFI BstAPI BstEII BstYI
Bsu36I BtgI BtsI CspCI EaeI EarI Eco57MI EcoNI
EcoO109I FspI FspAI HaeII Hin4I HincII KasI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PciI
PpiI PpuMI PstI PvuII ScaI SfcI SfoI SpeI
StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaI ApaLI AscI AseI AsiSI AvaI
BaeI BclI BfrBI BglI BglII Bme1580I BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI
BstBI BstXI BstZ17I BtgZI ClaI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI HindIII
HpaI KpnI MfeI MluI MmeI NdeI NheI NotI
NruI NsiI PacI PasI PflMI PfoI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SgfI
SgrAI SmaI SmlI SnaBI SphI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XhoI XmaI ZraI