Restriction map for
pFN21AE6757
(Linear) (Six-base) MAPSORT of: KIEE6757.seq Check: 2382 from: 1 to: 1599
KIEE6757.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AccI GT'mk_AC
Cuts at: 0 791 1599
Size: 791 808
Acc65I G'GTAC_C
Cuts at: 0 1131 1599
Size: 1131 468
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 223 274 1599
Size: 157 66 51 1325
Fragments arranged by size:
1325 157 66 51
ApoI r'AATT_y
Cuts at: 0 22 1599
Size: 22 1577
AvaI C'yCGr_G
Cuts at: 0 17 656 1177 1599
Size: 17 639 521 422
Fragments arranged by size:
639 521 422 17
BamHI G'GATC_C
Cuts at: 0 1327 1599
Size: 1327 272
BanI G'GyrC_C
Cuts at: 0 1131 1599
Size: 1131 468
BanII G_rGCy'C
Cuts at: 0 335 383 536 601 1081 1178 1184 1599
Size: 335 48 153 65 480 97 6 415
Fragments arranged by size:
480 415 335 153 97 65 48 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 627 1076 1599
Size: 627 449 523
Fragments arranged by size:
627 523 449
BbvCI CC'TCA_GC
Cuts at: 0 743 1118 1490 1599
Size: 743 375 372 109
Fragments arranged by size:
743 375 372 109
BclI T'GATC_A
Cuts at: 0 222 1155 1599
Size: 222 933 444
Fragments arranged by size:
933 444 222
BglI GCCn_nnn'nGGC
Cuts at: 0 650 985 1599
Size: 650 335 614
Fragments arranged by size:
650 614 335
BlpI GC'TnA_GC
Cuts at: 0 1412 1599
Size: 1412 187
BmgBI CAC'GTC
Cuts at: 0 114 1599
Size: 114 1485
BmrI ACTGGGnnnn_n'
Cuts at: 0 1083 1599
Size: 1083 516
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 337 367 919 1599
Size: 228 109 30 552 680
Fragments arranged by size:
680 552 228 109 30
Bpu10I CC'TnA_GC
Cuts at: 0 743 1118 1172 1490 1599
Size: 743 375 54 318 109
Fragments arranged by size:
743 375 318 109 54
BsaI GGTCTCn'nnnn_
Cuts at: 0 125 1599
Size: 125 1474
BsaHI Gr'CG_yC
Cuts at: 0 788 1599
Size: 788 811
BsaWI w'CCGG_w
Cuts at: 0 404 1010 1232 1599
Size: 404 606 222 367
Fragments arranged by size:
606 404 367 222
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 417 457 1051 1225 1599
Size: 417 40 594 174 374
Fragments arranged by size:
594 417 374 174 40
BseYI C'CCAG_C
Cuts at: 0 157 1599
Size: 157 1442
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 1599
Size: 262 1337
BsiEI CG_ry'CG
Cuts at: 0 190 1599
Size: 190 1409
BsiHKAI G_wGCw'C
Cuts at: 0 125 1178 1599
Size: 125 1053 421
Fragments arranged by size:
1053 421 125
Bsp1286I G_dGCh'C
Cuts at: 0 125 335 383 536 601 1081 1178 1184
Size: 125 210 48 153 65 480 97 6
Cuts at: 1184 1599
Size: 415
Fragments arranged by size:
480 415 210 153 125 97 65 48
6
BspEI T'CCGG_A
Cuts at: 0 404 1232 1599
Size: 404 828 367
Fragments arranged by size:
828 404 367
BspHI T'CATG_A
Cuts at: 0 833 1599
Size: 833 766
BsrBI CCG'CTC
Cuts at: 0 849 957 1401 1416 1599
Size: 849 108 444 15 183
Fragments arranged by size:
849 444 183 108 15
BsrDI GCAATG_nn'
Cuts at: 0 1066 1599
Size: 1066 533
BssSI C'ACGA_G
Cuts at: 0 124 1599
Size: 124 1475
BstBI TT'CG_AA
Cuts at: 0 462 1599
Size: 462 1137
BstXI CCAn_nnnn'nTGG
Cuts at: 0 114 700 1599
Size: 114 586 899
Fragments arranged by size:
899 586 114
BstYI r'GATC_y
Cuts at: 0 929 1327 1599
Size: 929 398 272
Fragments arranged by size:
929 398 272
BtgI C'CryG_G
Cuts at: 0 107 1599
Size: 107 1492
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 928 1599
Size: 928 671
BtsI GCAGTG_nn'
Cuts at: 0 1112 1599
Size: 1112 487
DraIII CAC_nnn'GTG
Cuts at: 0 213 1599
Size: 213 1386
DrdI GACnn_nn'nnGTC
Cuts at: 0 400 772 1599
Size: 400 372 827
Fragments arranged by size:
827 400 372
EaeI y'GGCC_r
Cuts at: 0 116 187 1599
Size: 116 71 1412
Fragments arranged by size:
1412 116 71
EagI C'GGCC_G
Cuts at: 0 187 1599
Size: 187 1412
EarI CTCTTCn'nnn_
Cuts at: 0 340 1037 1599
Size: 340 697 562
Fragments arranged by size:
697 562 340
EcoICRI GAG'CTC
Cuts at: 0 1176 1599
Size: 1176 423
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 223 228 274 337 367 919 1599
Size: 157 66 5 46 63 30 552 680
Fragments arranged by size:
680 552 157 66 63 46 30 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1489 1510 1599
Size: 1489 21 89
Fragments arranged by size:
1489 89 21
EcoRI G'AATT_C
Cuts at: 0 22 1599
Size: 22 1577
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 651 1599
Size: 651 948
HaeII r_GCGC'y
Cuts at: 0 243 1599
Size: 243 1356
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 407 439 1063 1095 1246 1278 1599
Size: 407 32 624 32 151 32 321
Fragments arranged by size:
624 407 321 151 32 32 32
HincII GTy'rAC
Cuts at: 0 792 1599
Size: 792 807
KpnI G_GTAC'C
Cuts at: 0 1135 1599
Size: 1135 464
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 947 1599
Size: 548 399 652
Fragments arranged by size:
652 548 399
MslI CAynn'nnrTG
Cuts at: 0 112 487 1599
Size: 112 375 1112
Fragments arranged by size:
1112 375 112
MspA1I CmG'CkG
Cuts at: 0 192 228 683 882 1599
Size: 192 36 455 199 717
Fragments arranged by size:
717 455 199 192 36
NcoI C'CATG_G
Cuts at: 0 107 1599
Size: 107 1492
PflMI CCAn_nnn'nTGG
Cuts at: 0 108 1599
Size: 108 1491
PfoI T'CCnGG_A
Cuts at: 0 244 935 1599
Size: 244 691 664
Fragments arranged by size:
691 664 244
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1079 1111 1160 1192 1599
Size: 1079 32 49 32 407
Fragments arranged by size:
1079 407 49 32 32
PspXI vC'TCGA_Gb
Cuts at: 0 1177 1599
Size: 1177 422
PstI C_TGCA'G
Cuts at: 0 673 881 1511 1599
Size: 673 208 630 88
Fragments arranged by size:
673 630 208 88
PvuII CAG'CTG
Cuts at: 0 228 1599
Size: 228 1371
SacI G_AGCT'C
Cuts at: 0 1178 1599
Size: 1178 421
SalI G'TCGA_C
Cuts at: 0 790 1599
Size: 790 809
SapI GCTCTTCn'nnn_
Cuts at: 0 340 1599
Size: 340 1259
SexAI A'CCwGG_T
Cuts at: 0 209 1599
Size: 209 1390
SfcI C'TryA_G
Cuts at: 0 669 877 1507 1599
Size: 669 208 630 92
Fragments arranged by size:
669 630 208 92
SmlI C'TyrA_G
Cuts at: 0 1177 1599
Size: 1177 422
StuI AGG'CCT
Cuts at: 0 290 662 1516 1599
Size: 290 372 854 83
Fragments arranged by size:
854 372 290 83
StyI C'CwwG_G
Cuts at: 0 107 663 1599
Size: 107 556 936
Fragments arranged by size:
936 556 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 20 1599
Size: 20 1579
Tth111I GACn'n_nGTC
Cuts at: 0 373 1599
Size: 373 1226
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 724 1374 1599
Size: 724 650 225
Fragments arranged by size:
724 650 225
XhoI C'TCGA_G
Cuts at: 0 1177 1599
Size: 1177 422
XmnI GAAnn'nnTTC
Cuts at: 0 634 1599
Size: 634 965
Enzymes that do cut:
AccI Acc65I AcuI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BclI BglI BlpI BmgBI BmrI BpmI
Bpu10I BsaI BsaHI BsaWI BseRI BseYI BsgI BsiEI
BsiHKAI Bsp1286I BspEI BspHI BsrBI BsrDI BssSI BstBI
BstXI BstYI BtgI BtgZI BtsI DraIII DrdI EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoRI FalI HaeII
Hin4I HincII KpnI MmeI MslI MspA1I NcoI PflMI
PfoI PpiI PspXI PstI PvuII SacI SalI SapI
SexAI SfcI SmlI StuI StyI TaqII Tth111I XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BciVI BfrBI BglII
Bme1580I BmtI BplI BpuEI BsaAI BsaBI BsaXI BsiWI
BsmI BsmBI BspMI BsrFI BsrGI BssHII BstAPI BstEII
BstZ17I Bsu36I ClaI CspCI DraI EciI EcoO109I EcoRV
FseI FspI FspAI HindIII HpaI KasI MfeI MluI
MscI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpuMI
PshAI PsiI PspOMI PsrI PvuI RsrII SacII SanDI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI XbaI
XmaI ZraI