Restriction map for
pFN21AE6751
(Linear) (Six-base) MAPSORT of: KIEE6751.seq Check: 6584 from: 1 to: 1779
KIEE6751.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
Acc65I G'GTAC_C
Cuts at: 0 271 1779
Size: 271 1508
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 310 712 1779
Size: 310 402 1067
Fragments arranged by size:
1067 402 310
AflII C'TTAA_G
Cuts at: 0 460 1779
Size: 460 1319
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 817 1779
Size: 817 962
AlwNI CAG_nnn'CTG
Cuts at: 0 735 1779
Size: 735 1044
ApaI G_GGCC'C
Cuts at: 0 562 1779
Size: 562 1217
ApoI r'AATT_y
Cuts at: 0 940 1779
Size: 940 839
AvaI C'yCGr_G
Cuts at: 0 589 805 1779
Size: 589 216 974
Fragments arranged by size:
974 589 216
AvrII C'CTAG_G
Cuts at: 0 1072 1779
Size: 1072 707
BamHI G'GATC_C
Cuts at: 0 1068 1779
Size: 1068 711
BanI G'GyrC_C
Cuts at: 0 168 182 271 425 801 1779
Size: 168 14 89 154 376 978
Fragments arranged by size:
978 376 168 154 89 14
BanII G_rGCy'C
Cuts at: 0 97 562 991 1498 1652 1779
Size: 97 465 429 507 154 127
Fragments arranged by size:
507 465 429 154 127 97
BbeI G_GCGC'C
Cuts at: 0 172 1779
Size: 172 1607
Bme1580I G_kGCm'C
Cuts at: 0 185 562 806 1779
Size: 185 377 244 973
Fragments arranged by size:
973 377 244 185
BmrI ACTGGGnnnn_n'
Cuts at: 0 642 798 1152 1492 1497 1727 1779
Size: 642 156 354 340 5 230 52
Fragments arranged by size:
642 354 340 230 156 52 5
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 649 1438 1779
Size: 82 567 789 341
Fragments arranged by size:
789 567 341 82
Bpu10I CC'TnA_GC
Cuts at: 0 1325 1779
Size: 1325 454
BsaI GGTCTCn'nnnn_
Cuts at: 0 1219 1249 1626 1779
Size: 1219 30 377 153
Fragments arranged by size:
1219 377 153 30
BsaAI yAC'GTr
Cuts at: 0 159 720 1779
Size: 159 561 1059
Fragments arranged by size:
1059 561 159
BsaHI Gr'CG_yC
Cuts at: 0 169 209 488 1133 1779
Size: 169 40 279 645 646
Fragments arranged by size:
646 645 279 169 40
BsaWI w'CCGG_w
Cuts at: 0 653 798 1772 1779
Size: 653 145 974 7
Fragments arranged by size:
974 653 145 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1411 1441 1779
Size: 1411 30 338
Fragments arranged by size:
1411 338 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 757 1650 1713 1779
Size: 757 893 63 66
Fragments arranged by size:
893 757 66 63
BseYI C'CCAG_C
Cuts at: 0 241 548 1263 1515 1614 1704 1779
Size: 241 307 715 252 99 90 75
Fragments arranged by size:
715 307 252 241 99 90 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 378 460 1257 1779
Size: 378 82 797 522
Fragments arranged by size:
797 522 378 82
BsiEI CG_ry'CG
Cuts at: 0 251 332 345 1779
Size: 251 81 13 1434
Fragments arranged by size:
1434 251 81 13
BsiHKAI G_wGCw'C
Cuts at: 0 284 1326 1652 1779
Size: 284 1042 326 127
Fragments arranged by size:
1042 326 284 127
BsmI GAATG_Cn'
Cuts at: 0 794 1779
Size: 794 985
Bsp1286I G_dGCh'C
Cuts at: 0 97 185 284 562 806 991 1326 1498
Size: 97 88 99 278 244 185 335 172
Cuts at: 1498 1652 1779
Size: 154 127
Fragments arranged by size:
335 278 244 185 172 154 127 99
97 88
BspEI T'CCGG_A
Cuts at: 0 653 1779
Size: 653 1126
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1288 1365 1779
Size: 1288 77 414
Fragments arranged by size:
1288 414 77
BsrBI CCG'CTC
Cuts at: 0 596 1779
Size: 596 1183
BsrFI r'CCGG_y
Cuts at: 0 106 382 903 979 1779
Size: 106 276 521 76 800
Fragments arranged by size:
800 521 276 106 76
BssHII G'CGCG_C
Cuts at: 0 129 1779
Size: 129 1650
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1280 1779
Size: 1280 499
BstEII G'GTnAC_C
Cuts at: 0 543 1779
Size: 543 1236
BstXI CCAn_nnnn'nTGG
Cuts at: 0 611 1053 1779
Size: 611 442 726
Fragments arranged by size:
726 611 442
BstYI r'GATC_y
Cuts at: 0 1068 1779
Size: 1068 711
Bsu36I CC'TnA_GG
Cuts at: 0 533 689 1779
Size: 533 156 1090
Fragments arranged by size:
1090 533 156
BtgI C'CryG_G
Cuts at: 0 314 413 468 1097 1474 1779
Size: 314 99 55 629 377 305
Fragments arranged by size:
629 377 314 305 99 55
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 125 1779
Size: 125 1654
BtsI GCAGTG_nn'
Cuts at: 0 1229 1361 1779
Size: 1229 132 418
Fragments arranged by size:
1229 418 132
DraIII CAC_nnn'GTG
Cuts at: 0 432 1779
Size: 432 1347
EaeI y'GGCC_r
Cuts at: 0 248 901 905 1032 1358 1779
Size: 248 653 4 127 326 421
Fragments arranged by size:
653 421 326 248 127 4
EagI C'GGCC_G
Cuts at: 0 248 1779
Size: 248 1531
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 10 220 1779
Size: 10 210 1559
Fragments arranged by size:
1559 210 10
EcoICRI GAG'CTC
Cuts at: 0 1650 1779
Size: 1650 129
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 310 649 712 1438 1779
Size: 82 228 339 63 726 341
Fragments arranged by size:
726 341 339 228 82 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 532 1126 1632 1691 1779
Size: 532 594 506 59 88
Fragments arranged by size:
594 532 506 88 59
EcoO109I rG'GnC_Cy
Cuts at: 0 238 1708 1779
Size: 238 1470 71
Fragments arranged by size:
1470 238 71
FseI GG_CCGG'CC
Cuts at: 0 907 1779
Size: 907 872
HaeII r_GCGC'y
Cuts at: 0 172 1779
Size: 172 1607
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 334 366 585 617 877 909 1411 1443
Size: 334 32 219 32 260 32 502 32
Cuts at: 1443 1779
Size: 336
Fragments arranged by size:
502 336 334 260 219 32 32 32
32
HincII GTy'rAC
Cuts at: 0 852 1779
Size: 852 927
KasI G'GCGC_C
Cuts at: 0 168 1779
Size: 168 1611
KpnI G_GTAC'C
Cuts at: 0 275 1779
Size: 275 1504
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 539 1779
Size: 539 1240
MscI TGG'CCA
Cuts at: 0 1034 1360 1779
Size: 1034 326 419
Fragments arranged by size:
1034 419 326
MslI CAynn'nnrTG
Cuts at: 0 799 1779
Size: 799 980
MspA1I CmG'CkG
Cuts at: 0 316 470 669 1433 1779
Size: 316 154 199 764 346
Fragments arranged by size:
764 346 316 199 154
NaeI GCC'GGC
Cuts at: 0 108 905 981 1779
Size: 108 797 76 798
Fragments arranged by size:
798 797 108 76
NarI GG'CG_CC
Cuts at: 0 169 1779
Size: 169 1610
NcoI C'CATG_G
Cuts at: 0 1474 1779
Size: 1474 305
NgoMIV G'CCGG_C
Cuts at: 0 106 903 979 1779
Size: 106 797 76 800
Fragments arranged by size:
800 797 106 76
NspI r_CATG'y
Cuts at: 0 1471 1779
Size: 1471 308
PflMI CCAn_nnn'nTGG
Cuts at: 0 956 1754 1779
Size: 956 798 25
Fragments arranged by size:
956 798 25
PfoI T'CCnGG_A
Cuts at: 0 59 374 610 1747 1779
Size: 59 315 236 1137 32
Fragments arranged by size:
1137 315 236 59 32
PpuMI rG'GwC_Cy
Cuts at: 0 238 1708 1779
Size: 238 1470 71
Fragments arranged by size:
1470 238 71
PspOMI G'GGCC_C
Cuts at: 0 558 1779
Size: 558 1221
PstI C_TGCA'G
Cuts at: 0 1574 1779
Size: 1574 205
PvuII CAG'CTG
Cuts at: 0 1433 1779
Size: 1433 346
SacI G_AGCT'C
Cuts at: 0 1652 1779
Size: 1652 127
SacII CC_GC'GG
Cuts at: 0 317 471 1779
Size: 317 154 1308
Fragments arranged by size:
1308 317 154
SfcI C'TryA_G
Cuts at: 0 84 1570 1779
Size: 84 1486 209
Fragments arranged by size:
1486 209 84
SfoI GGC'GCC
Cuts at: 0 170 1779
Size: 170 1609
SmaI CCC'GGG
Cuts at: 0 807 1779
Size: 807 972
SmlI C'TyrA_G
Cuts at: 0 460 589 1779
Size: 460 129 1190
Fragments arranged by size:
1190 460 129
SphI G_CATG'C
Cuts at: 0 1471 1779
Size: 1471 308
StyI C'CwwG_G
Cuts at: 0 539 617 1072 1205 1474 1538 1779
Size: 539 78 455 133 269 64 241
Fragments arranged by size:
539 455 269 241 133 78 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 162 346 359 562 1779
Size: 162 184 13 203 1217
Fragments arranged by size:
1217 203 184 162 13
TatI w'GTAC_w
Cuts at: 0 188 1779
Size: 188 1591
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1014 1046 1779
Size: 1014 32 733
Fragments arranged by size:
1014 733 32
Tth111I GACn'n_nGTC
Cuts at: 0 346 1779
Size: 346 1433
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 668 1755 1779
Size: 668 1087 24
Fragments arranged by size:
1087 668 24
XhoI C'TCGA_G
Cuts at: 0 589 1779
Size: 589 1190
XmaI C'CCGG_G
Cuts at: 0 805 1779
Size: 805 974
XmnI GAAnn'nnTTC
Cuts at: 0 710 1779
Size: 710 1069
Enzymes that do cut:
Acc65I AcuI AflII AlfI AlwNI ApaI ApoI AvaI
AvrII BamHI BanI BanII BbeI Bme1580I BmrI BpmI
Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrFI BssHII BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI DraIII EaeI EagI EciI EcoICRI Eco57MI
EcoNI EcoO109I FseI HaeII Hin4I HincII KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacI
SacII SfcI SfoI SmaI SmlI SphI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflIII AgeI AhdI
AleI AloI ApaLI AscI AseI AsiSI BaeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI BpuEI BsaBI BsiWI BsmBI BspHI
BsrDI BsrGI BssSI BstBI BstZ17I ClaI CspCI DraI
DrdI EarI EcoRI EcoRV FalI FspI FspAI HindIII
HpaI MfeI MluI NdeI NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SapI SbfI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SrfI
SspI StuI SwaI XbaI ZraI