Restriction map for
pFN21AE6750
(Linear) (Six-base) MAPSORT of: KIEE6750.seq Check: 3451 from: 1 to: 1767
KIEE6750.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 1767
Size: 322 1445
AflIII A'CryG_T
Cuts at: 0 894 1238 1767
Size: 894 344 529
Fragments arranged by size:
894 529 344
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 270 851 1767
Size: 270 581 916
Fragments arranged by size:
916 581 270
AlwNI CAG_nnn'CTG
Cuts at: 0 38 1767
Size: 38 1729
ApaLI G'TGCA_C
Cuts at: 0 255 1767
Size: 255 1512
ApoI r'AATT_y
Cuts at: 0 1115 1767
Size: 1115 652
AvaI C'yCGr_G
Cuts at: 0 931 1168 1186 1767
Size: 931 237 18 581
Fragments arranged by size:
931 581 237 18
BamHI G'GATC_C
Cuts at: 0 995 1157 1767
Size: 995 162 610
Fragments arranged by size:
995 610 162
BanI G'GyrC_C
Cuts at: 0 75 1275 1468 1742 1767
Size: 75 1200 193 274 25
Fragments arranged by size:
1200 274 193 75 25
BanII G_rGCy'C
Cuts at: 0 1223 1595 1767
Size: 1223 372 172
Fragments arranged by size:
1223 372 172
BbeI G_GCGC'C
Cuts at: 0 79 1767
Size: 79 1688
BbsI GAAGACnn'nnnn_
Cuts at: 0 355 1767
Size: 355 1412
BclI T'GATC_A
Cuts at: 0 468 1767
Size: 468 1299
BfrBI ATG'CAT
Cuts at: 0 1362 1767
Size: 1362 405
BglI GCCn_nnn'nGGC
Cuts at: 0 697 1767
Size: 697 1070
Bme1580I G_kGCm'C
Cuts at: 0 259 1169 1280 1767
Size: 259 910 111 487
Fragments arranged by size:
910 487 259 111
BmgBI CAC'GTC
Cuts at: 0 1014 1239 1767
Size: 1014 225 528
Fragments arranged by size:
1014 528 225
BmrI ACTGGGnnnn_n'
Cuts at: 0 1089 1654 1767
Size: 1089 565 113
Fragments arranged by size:
1089 565 113
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 377 1716 1767
Size: 377 1339 51
Fragments arranged by size:
1339 377 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1533 1767
Size: 1533 234
BsaI GGTCTCn'nnnn_
Cuts at: 0 451 1547 1558 1692 1767
Size: 451 1096 11 134 75
Fragments arranged by size:
1096 451 134 75 11
BsaHI Gr'CG_yC
Cuts at: 0 76 1441 1767
Size: 76 1365 326
Fragments arranged by size:
1365 326 76
BsaWI w'CCGG_w
Cuts at: 0 346 1767
Size: 346 1421
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 823 853 1767
Size: 823 30 914
Fragments arranged by size:
914 823 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 697 700 865 985 1767
Size: 697 3 165 120 782
Fragments arranged by size:
782 697 165 120 3
BseYI C'CCAG_C
Cuts at: 0 548 1683 1767
Size: 548 1135 84
Fragments arranged by size:
1135 548 84
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1366 1767
Size: 1366 401
BsiEI CG_ry'CG
Cuts at: 0 112 701 1202 1206 1767
Size: 112 589 501 4 561
Fragments arranged by size:
589 561 501 112 4
BsiHKAI G_wGCw'C
Cuts at: 0 259 738 1196 1767
Size: 259 479 458 571
Fragments arranged by size:
571 479 458 259
BsmI GAATG_Cn'
Cuts at: 0 521 1767
Size: 521 1246
BsmBI CGTCTCn'nnnn_
Cuts at: 0 669 1767
Size: 669 1098
Bsp1286I G_dGCh'C
Cuts at: 0 259 738 1169 1196 1223 1280 1595 1767
Size: 259 479 431 27 27 57 315 172
Fragments arranged by size:
479 431 315 259 172 57 27 27
BsrDI GCAATG_nn'
Cuts at: 0 471 1767
Size: 471 1296
BsrFI r'CCGG_y
Cuts at: 0 675 1201 1465 1767
Size: 675 526 264 302
Fragments arranged by size:
675 526 302 264
BssSI C'ACGA_G
Cuts at: 0 666 1224 1348 1767
Size: 666 558 124 419
Fragments arranged by size:
666 558 419 124
BstEII G'GTnAC_C
Cuts at: 0 325 1767
Size: 325 1442
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1102 1767
Size: 1102 665
BstYI r'GATC_y
Cuts at: 0 995 1157 1172 1767
Size: 995 162 15 595
Fragments arranged by size:
995 595 162 15
Bsu36I CC'TnA_GG
Cuts at: 0 1747 1767
Size: 1747 20
BtgI C'CryG_G
Cuts at: 0 945 1250 1702 1767
Size: 945 305 452 65
Fragments arranged by size:
945 452 305 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 64 1767
Size: 64 1703
DraIII CAC_nnn'GTG
Cuts at: 0 1437 1767
Size: 1437 330
EaeI y'GGCC_r
Cuts at: 0 698 1199 1203 1299 1767
Size: 698 501 4 96 468
Fragments arranged by size:
698 501 468 96 4
EagI C'GGCC_G
Cuts at: 0 698 1199 1203 1767
Size: 698 501 4 564
Fragments arranged by size:
698 564 501 4
EarI CTCTTCn'nnn_
Cuts at: 0 604 931 1767
Size: 604 327 836
Fragments arranged by size:
836 604 327
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 90 148 1767
Size: 90 58 1619
Fragments arranged by size:
1619 90 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 377 1716 1767
Size: 322 55 1339 51
Fragments arranged by size:
1339 322 55 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 208 1767
Size: 208 1559
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1574 1767
Size: 1574 193
FseI GG_CCGG'CC
Cuts at: 0 1205 1767
Size: 1205 562
HaeII r_GCGC'y
Cuts at: 0 79 1767
Size: 79 1688
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 101 133 823 855 1767
Size: 101 32 690 32 912
Fragments arranged by size:
912 690 101 32 32
HincII GTy'rAC
Cuts at: 0 573 1767
Size: 573 1194
KasI G'GCGC_C
Cuts at: 0 75 1767
Size: 75 1692
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 1034 1767
Size: 391 643 733
Fragments arranged by size:
733 643 391
MslI CAynn'nnrTG
Cuts at: 0 252 1367 1767
Size: 252 1115 400
Fragments arranged by size:
1115 400 252
MspA1I CmG'CkG
Cuts at: 0 548 1181 1647 1674 1767
Size: 548 633 466 27 93
Fragments arranged by size:
633 548 466 93 27
NaeI GCC'GGC
Cuts at: 0 1203 1767
Size: 1203 564
NarI GG'CG_CC
Cuts at: 0 76 1767
Size: 76 1691
NgoMIV G'CCGG_C
Cuts at: 0 1201 1767
Size: 1201 566
NsiI A_TGCA'T
Cuts at: 0 1364 1767
Size: 1364 403
PflMI CCAn_nnn'nTGG
Cuts at: 0 609 1767
Size: 609 1158
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 572 604 1582 1614 1767
Size: 572 32 978 32 153
Fragments arranged by size:
978 572 153 32 32
PsiI TTA'TAA
Cuts at: 0 1121 1767
Size: 1121 646
PstI C_TGCA'G
Cuts at: 0 830 1673 1712 1767
Size: 830 843 39 55
Fragments arranged by size:
843 830 55 39
PvuII CAG'CTG
Cuts at: 0 1674 1767
Size: 1674 93
SfcI C'TryA_G
Cuts at: 0 826 1669 1708 1767
Size: 826 843 39 59
Fragments arranged by size:
843 826 59 39
SfoI GGC'GCC
Cuts at: 0 77 1767
Size: 77 1690
SmaI CCC'GGG
Cuts at: 0 1170 1188 1767
Size: 1170 18 579
Fragments arranged by size:
1170 579 18
SmlI C'TyrA_G
Cuts at: 0 1548 1767
Size: 1548 219
SspI AAT'ATT
Cuts at: 0 195 1767
Size: 195 1572
StyI C'CwwG_G
Cuts at: 0 141 614 1767
Size: 141 473 1153
Fragments arranged by size:
1153 473 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 126 523 721 1108 1767
Size: 126 397 198 387 659
Fragments arranged by size:
659 397 387 198 126
TatI w'GTAC_w
Cuts at: 0 60 1767
Size: 60 1707
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1051 1083 1767
Size: 1051 32 684
Fragments arranged by size:
1051 684 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 952 1230 1767
Size: 952 278 537
Fragments arranged by size:
952 537 278
XmaI C'CCGG_G
Cuts at: 0 1168 1186 1767
Size: 1168 18 581
Fragments arranged by size:
1168 581 18
Enzymes that do cut:
AcuI AflIII AlfI AlwNI ApaLI ApoI AvaI BamHI
BanI BanII BbeI BbsI BclI BfrBI BglI Bme1580I
BmgBI BmrI BpmI BpuEI BsaI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BsrDI BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI
BtgZI DraIII EaeI EagI EarI EciI Eco57MI EcoNI
FalI FseI HaeII Hin4I HincII KasI MmeI MslI
MspA1I NaeI NarI NgoMIV NsiI PflMI PpiI PsiI
PstI PvuII SfcI SfoI SmaI SmlI SspI StyI
TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AloI ApaI AscI AseI AsiSI AvrII
BaeI BbvCI BcgI BciVI BglII BlpI BmtI BplI
Bpu10I BsaAI BsaBI BsiWI BspEI BspHI BspMI BsrBI
BsrGI BssHII BstAPI BstBI BstZ17I BtsI ClaI CspCI
DraI DrdI EcoICRI EcoO109I EcoRI EcoRV FspI FspAI
HindIII HpaI KpnI MfeI MluI MscI NcoI NdeI
NheI NotI NruI NspI PacI PasI PciI PfoI
PmeI PmlI PpuMI PshAI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI
StuI SwaI Tth111I XbaI XhoI XmnI ZraI