Restriction map for
pFN21AE6749
(Linear) (Six-base) MAPSORT of: KIEE6749.seq Check: 4795 from: 1 to: 1626
KIEE6749.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 735 1626
Size: 735 891
Acc65I G'GTAC_C
Cuts at: 0 258 1626
Size: 258 1368
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 311 1046 1626
Size: 35 276 735 580
Fragments arranged by size:
735 580 276 35
AfeI AGC'GCT
Cuts at: 0 1024 1626
Size: 1024 602
AflIII A'CryG_T
Cuts at: 0 1367 1626
Size: 1367 259
AgeI A'CCGG_T
Cuts at: 0 595 1626
Size: 595 1031
AlwNI CAG_nnn'CTG
Cuts at: 0 189 1056 1626
Size: 189 867 570
Fragments arranged by size:
867 570 189
ApoI r'AATT_y
Cuts at: 0 553 576 1626
Size: 553 23 1050
Fragments arranged by size:
1050 553 23
AvaI C'yCGr_G
Cuts at: 0 1003 1410 1626
Size: 1003 407 216
Fragments arranged by size:
1003 407 216
AvrII C'CTAG_G
Cuts at: 0 1296 1626
Size: 1296 330
BanI G'GyrC_C
Cuts at: 0 258 619 736 1626
Size: 258 361 117 890
Fragments arranged by size:
890 361 258 117
BanII G_rGCy'C
Cuts at: 0 730 1004 1417 1468 1626
Size: 730 274 413 51 158
Fragments arranged by size:
730 413 274 158 51
BbeI G_GCGC'C
Cuts at: 0 740 1626
Size: 740 886
BbsI GAAGACnn'nnnn_
Cuts at: 0 147 750 1052 1079 1364 1626
Size: 147 603 302 27 285 262
Fragments arranged by size:
603 302 285 262 147 27
BbvCI CC'TCA_GC
Cuts at: 0 1223 1389 1618 1626
Size: 1223 166 229 8
Fragments arranged by size:
1223 229 166 8
BclI T'GATC_A
Cuts at: 0 1247 1626
Size: 1247 379
BlpI GC'TnA_GC
Cuts at: 0 221 781 1626
Size: 221 560 845
Fragments arranged by size:
845 560 221
BmrI ACTGGGnnnn_n'
Cuts at: 0 1133 1205 1626
Size: 1133 72 421
Fragments arranged by size:
1133 421 72
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 1590 1626
Size: 211 1379 36
Fragments arranged by size:
1379 211 36
Bpu10I CC'TnA_GC
Cuts at: 0 1223 1389 1618 1626
Size: 1223 166 229 8
Fragments arranged by size:
1223 229 166 8
BsaBI GATnn'nnATC
Cuts at: 0 949 1626
Size: 949 677
BsaHI Gr'CG_yC
Cuts at: 0 329 737 827 1626
Size: 329 408 90 799
Fragments arranged by size:
799 408 329 90
BsaWI w'CCGG_w
Cuts at: 0 595 881 1626
Size: 595 286 745
Fragments arranged by size:
745 595 286
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1269 1299 1626
Size: 1269 30 327
Fragments arranged by size:
1269 327 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 652 1059 1163 1587 1626
Size: 652 407 104 424 39
Fragments arranged by size:
652 424 407 104 39
BseYI C'CCAG_C
Cuts at: 0 1049 1626
Size: 1049 577
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1113 1626
Size: 1113 513
BsiHKAI G_wGCw'C
Cuts at: 0 730 782 1626
Size: 730 52 844
Fragments arranged by size:
844 730 52
BsmI GAATG_Cn'
Cuts at: 0 545 1626
Size: 545 1081
BsmBI CGTCTCn'nnnn_
Cuts at: 0 316 818 1626
Size: 316 502 808
Fragments arranged by size:
808 502 316
Bsp1286I G_dGCh'C
Cuts at: 0 730 782 1004 1417 1468 1626
Size: 730 52 222 413 51 158
Fragments arranged by size:
730 413 222 158 52 51
BspHI T'CATG_A
Cuts at: 0 644 1250 1626
Size: 644 606 376
Fragments arranged by size:
644 606 376
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 735 744 1626
Size: 735 9 882
Fragments arranged by size:
882 735 9
BsrBI CCG'CTC
Cuts at: 0 1407 1626
Size: 1407 219
BsrDI GCAATG_nn'
Cuts at: 0 373 981 1626
Size: 373 608 645
Fragments arranged by size:
645 608 373
BsrFI r'CCGG_y
Cuts at: 0 595 1183 1564 1626
Size: 595 588 381 62
Fragments arranged by size:
595 588 381 62
BsrGI T'GTAC_A
Cuts at: 0 90 1626
Size: 90 1536
BstAPI GCAn_nnn'nTGC
Cuts at: 0 201 1626
Size: 201 1425
DraIII CAC_nnn'GTG
Cuts at: 0 216 1626
Size: 216 1410
DrdI GACnn_nn'nnGTC
Cuts at: 0 151 1626
Size: 151 1475
EaeI y'GGCC_r
Cuts at: 0 76 476 720 803 1017 1181 1379 1473
Size: 76 400 244 83 214 164 198 94
Cuts at: 1473 1626
Size: 153
Fragments arranged by size:
400 244 214 198 164 153 94 83
76
EarI CTCTTCn'nnn_
Cuts at: 0 27 1083 1626
Size: 27 1056 543
Fragments arranged by size:
1056 543 27
EcoICRI GAG'CTC
Cuts at: 0 728 1626
Size: 728 898
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 211 311 1046 1590 1626
Size: 35 176 100 735 544 36
Fragments arranged by size:
735 544 176 100 36 35
EcoNI CCTnn'n_nnAGG
Cuts at: 0 247 1626
Size: 247 1379
EcoO109I rG'GnC_Cy
Cuts at: 0 423 693 809 1626
Size: 423 270 116 817
Fragments arranged by size:
817 423 270 116
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 774 1626
Size: 774 852
HaeII r_GCGC'y
Cuts at: 0 740 1026 1168 1626
Size: 740 286 142 458
Fragments arranged by size:
740 458 286 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 991 1023 1404 1436 1626
Size: 991 32 381 32 190
Fragments arranged by size:
991 381 190 32 32
HincII GTy'rAC
Cuts at: 0 303 1626
Size: 303 1323
KasI G'GCGC_C
Cuts at: 0 736 1626
Size: 736 890
KpnI G_GTAC'C
Cuts at: 0 262 1626
Size: 262 1364
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 1626
Size: 154 1472
MscI TGG'CCA
Cuts at: 0 478 805 1019 1626
Size: 478 327 214 607
Fragments arranged by size:
607 478 327 214
MslI CAynn'nnrTG
Cuts at: 0 745 1626
Size: 745 881
MspA1I CmG'CkG
Cuts at: 0 186 340 662 801 1626
Size: 186 154 322 139 825
Fragments arranged by size:
825 322 186 154 139
NaeI GCC'GGC
Cuts at: 0 1185 1626
Size: 1185 441
NarI GG'CG_CC
Cuts at: 0 737 1626
Size: 737 889
NgoMIV G'CCGG_C
Cuts at: 0 1183 1626
Size: 1183 443
NspI r_CATG'y
Cuts at: 0 974 1371 1626
Size: 974 397 255
Fragments arranged by size:
974 397 255
PciI A'CATG_T
Cuts at: 0 1367 1626
Size: 1367 259
PpuMI rG'GwC_Cy
Cuts at: 0 423 693 809 1626
Size: 423 270 116 817
Fragments arranged by size:
817 423 270 116
PshAI GACnn'nnGTC
Cuts at: 0 328 1626
Size: 328 1298
PstI C_TGCA'G
Cuts at: 0 419 1626
Size: 419 1207
PvuII CAG'CTG
Cuts at: 0 186 1626
Size: 186 1440
SacI G_AGCT'C
Cuts at: 0 730 1626
Size: 730 896
ScaI AGT'ACT
Cuts at: 0 837 1626
Size: 837 789
SexAI A'CCwGG_T
Cuts at: 0 146 1270 1626
Size: 146 1124 356
Fragments arranged by size:
1124 356 146
SfcI C'TryA_G
Cuts at: 0 415 490 1494 1626
Size: 415 75 1004 132
Fragments arranged by size:
1004 415 132 75
SfoI GGC'GCC
Cuts at: 0 738 1626
Size: 738 888
SmlI C'TyrA_G
Cuts at: 0 1410 1626
Size: 1410 216
SspI AAT'ATT
Cuts at: 0 370 1626
Size: 370 1256
StyI C'CwwG_G
Cuts at: 0 534 1296 1482 1626
Size: 534 762 186 144
Fragments arranged by size:
762 534 186 144
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1495 1626
Size: 1495 131
TatI w'GTAC_w
Cuts at: 0 90 112 835 1626
Size: 90 22 723 791
Fragments arranged by size:
791 723 90 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1357 1389 1626
Size: 1357 32 237
Fragments arranged by size:
1357 237 32
Tth111I GACn'n_nGTC
Cuts at: 0 1032 1626
Size: 1032 594
XhoI C'TCGA_G
Cuts at: 0 1410 1626
Size: 1410 216
XmnI GAAnn'nnTTC
Cuts at: 0 406 1626
Size: 406 1220
Enzymes that do cut:
AarI Acc65I AcuI AfeI AflIII AgeI AlwNI ApoI
AvaI AvrII BanI BanII BbeI BbsI BbvCI BclI
BlpI BmrI BpmI Bpu10I BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstAPI DraIII DrdI
EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII KasI KpnI MmeI MscI MslI MspA1I
NaeI NarI NgoMIV NspI PciI PpuMI PshAI PstI
PvuII SacI ScaI SexAI SfcI SfoI SmlI SspI
StyI TaqII TatI TstI Tth111I XhoI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AhdI AleI AlfI AloI
ApaI ApaLI AscI AseI AsiSI BaeI BamHI BcgI
BciVI BfrBI BglI BglII Bme1580I BmgBI BmtI BplI
BpuEI BsaI BsaAI BsiEI BsiWI BspEI BssHII BssSI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI EagI EciI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI MfeI MluI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI
SwaI XbaI XcmI XmaI ZraI