Restriction map for
pFN21AE6744
(Linear) (Six-base) MAPSORT of: KIEE6744.seq Check: 7474 from: 1 to: 2919
KIEE6744.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AccI GT'mk_AC
Cuts at: 0 257 785 2919
Size: 257 528 2134
Fragments arranged by size:
2134 528 257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1009 1214 1314 1400 1496 1526 1965 2919
Size: 1009 205 100 86 96 30 439 954
Fragments arranged by size:
1009 954 439 205 100 96 86 30
AflII C'TTAA_G
Cuts at: 0 997 2919
Size: 997 1922
AflIII A'CryG_T
Cuts at: 0 260 2919
Size: 260 2659
AleI CACnn'nnGTG
Cuts at: 0 511 1195 2919
Size: 511 684 1724
Fragments arranged by size:
1724 684 511
AlwNI CAG_nnn'CTG
Cuts at: 0 1254 2061 2919
Size: 1254 807 858
Fragments arranged by size:
1254 858 807
ApaI G_GGCC'C
Cuts at: 0 2819 2919
Size: 2819 100
ApoI r'AATT_y
Cuts at: 0 903 1372 1467 1786 2161 2919
Size: 903 469 95 319 375 758
Fragments arranged by size:
903 758 469 375 319 95
AseI AT'TA_AT
Cuts at: 0 1033 2919
Size: 1033 1886
AvaI C'yCGr_G
Cuts at: 0 1697 2919
Size: 1697 1222
BanI G'GyrC_C
Cuts at: 0 567 2105 2919
Size: 567 1538 814
Fragments arranged by size:
1538 814 567
BanII G_rGCy'C
Cuts at: 0 1698 1784 1886 2819 2919
Size: 1698 86 102 933 100
Fragments arranged by size:
1698 933 102 100 86
BbeI G_GCGC'C
Cuts at: 0 2109 2919
Size: 2109 810
BbsI GAAGACnn'nnnn_
Cuts at: 0 600 1971 2200 2625 2919
Size: 600 1371 229 425 294
Fragments arranged by size:
1371 600 425 294 229
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1720 1754 2919
Size: 1720 34 1165
Fragments arranged by size:
1720 1165 34
BclI T'GATC_A
Cuts at: 0 2141 2919
Size: 2141 778
BglII A'GATC_T
Cuts at: 0 1223 2919
Size: 1223 1696
Bme1580I G_kGCm'C
Cuts at: 0 2819 2919
Size: 2819 100
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 949 1139 1276 1712 2250 2911 2919
Size: 648 301 190 137 436 538 661 8
Fragments arranged by size:
661 648 538 436 301 190 137 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 1891 2919
Size: 308 1583 1028
Fragments arranged by size:
1583 1028 308
BsaI GGTCTCn'nnnn_
Cuts at: 0 355 485 2450 2919
Size: 355 130 1965 469
Fragments arranged by size:
1965 469 355 130
BsaAI yAC'GTr
Cuts at: 0 2328 2919
Size: 2328 591
BsaHI Gr'CG_yC
Cuts at: 0 2106 2591 2919
Size: 2106 485 328
Fragments arranged by size:
2106 485 328
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1471 1855 2095 2824 2874 2919
Size: 1471 384 240 729 50 45
Fragments arranged by size:
1471 729 384 240 50 45
BseYI C'CCAG_C
Cuts at: 0 1357 2266 2399 2808 2919
Size: 1357 909 133 409 111
Fragments arranged by size:
1357 909 409 133 111
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2463 2919
Size: 2463 456
BsiHKAI G_wGCw'C
Cuts at: 0 921 1698 1784 2126 2858 2919
Size: 921 777 86 342 732 61
Fragments arranged by size:
921 777 732 342 86 61
BsmI GAATG_Cn'
Cuts at: 0 526 1094 2919
Size: 526 568 1825
Fragments arranged by size:
1825 568 526
Bsp1286I G_dGCh'C
Cuts at: 0 921 1698 1784 1886 2126 2819 2858 2919
Size: 921 777 86 102 240 693 39 61
Fragments arranged by size:
921 777 693 240 102 86 61 39
BspHI T'CATG_A
Cuts at: 0 1112 2426 2919
Size: 1112 1314 493
Fragments arranged by size:
1314 1112 493
BsrDI GCAATG_nn'
Cuts at: 0 1494 2030 2090 2919
Size: 1494 536 60 829
Fragments arranged by size:
1494 829 536 60
BsrGI T'GTAC_A
Cuts at: 0 2 2870 2919
Size: 2 2868 49
Fragments arranged by size:
2868 49 2
BssSI C'ACGA_G
Cuts at: 0 1180 2919
Size: 1180 1739
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2562 2919
Size: 2562 357
BstYI r'GATC_y
Cuts at: 0 383 427 1223 1564 2919
Size: 383 44 796 341 1355
Fragments arranged by size:
1355 796 383 341 44
BtgI C'CryG_G
Cuts at: 0 251 2919
Size: 251 2668
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2646 2919
Size: 2646 273
BtsI GCAGTG_nn'
Cuts at: 0 482 1574 2919
Size: 482 1092 1345
Fragments arranged by size:
1345 1092 482
DraI TTT'AAA
Cuts at: 0 1136 2919
Size: 1136 1783
DraIII CAC_nnn'GTG
Cuts at: 0 556 2919
Size: 556 2363
EaeI y'GGCC_r
Cuts at: 0 248 471 1968 2919
Size: 248 223 1497 951
Fragments arranged by size:
1497 951 248 223
EarI CTCTTCn'nnn_
Cuts at: 0 2072 2294 2743 2919
Size: 2072 222 449 176
Fragments arranged by size:
2072 449 222 176
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2799 2919
Size: 2799 120
EcoICRI GAG'CTC
Cuts at: 0 1696 1782 2919
Size: 1696 86 1137
Fragments arranged by size:
1696 1137 86
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 648 949 1009 1139 1214 1276 1314 1400
Size: 648 301 60 130 75 62 38 86
Cuts at: 1400 1496 1526 1712 1965 2250 2911 2919
Size: 96 30 186 253 285 661 8
Fragments arranged by size:
661 648 301 285 253 186 130 96
86 75 62 60 38 30 8
EcoO109I rG'GnC_Cy
Cuts at: 0 2358 2816 2919
Size: 2358 458 103
Fragments arranged by size:
2358 458 103
EcoRI G'AATT_C
Cuts at: 0 1786 2161 2919
Size: 1786 375 758
Fragments arranged by size:
1786 758 375
EcoRV GAT'ATC
Cuts at: 0 1449 2919
Size: 1449 1470
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 724 1245 1881 2919
Size: 724 521 636 1038
Fragments arranged by size:
1038 724 636 521
HaeII r_GCGC'y
Cuts at: 0 2109 2919
Size: 2109 810
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 263 295 832 864 1418 1450 2919
Size: 263 32 537 32 554 32 1469
Fragments arranged by size:
1469 554 537 263 32 32 32
HincII GTy'rAC
Cuts at: 0 1188 1326 2919
Size: 1188 138 1593
Fragments arranged by size:
1593 1188 138
HindIII A'AGCT_T
Cuts at: 0 1508 2919
Size: 1508 1411
KasI G'GCGC_C
Cuts at: 0 2105 2919
Size: 2105 814
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2114 2919
Size: 2114 805
MscI TGG'CCA
Cuts at: 0 250 473 1970 2919
Size: 250 223 1497 949
Fragments arranged by size:
1497 949 250 223
MslI CAynn'nnrTG
Cuts at: 0 511 602 1195 1248 1646 1669 2425 2761
Size: 511 91 593 53 398 23 756 336
Cuts at: 2761 2919
Size: 158
Fragments arranged by size:
756 593 511 398 336 158 91 53
23
MspA1I CmG'CkG
Cuts at: 0 1150 2228 2919
Size: 1150 1078 691
Fragments arranged by size:
1150 1078 691
NarI GG'CG_CC
Cuts at: 0 2106 2919
Size: 2106 813
NcoI C'CATG_G
Cuts at: 0 251 2919
Size: 251 2668
NspI r_CATG'y
Cuts at: 0 12 264 1147 1179 2919
Size: 12 252 883 32 1740
Fragments arranged by size:
1740 883 252 32 12
PasI CC'CwG_GG
Cuts at: 0 423 2919
Size: 423 2496
PciI A'CATG_T
Cuts at: 0 260 2919
Size: 260 2659
PpuMI rG'GwC_Cy
Cuts at: 0 2358 2919
Size: 2358 561
PshAI GACnn'nnGTC
Cuts at: 0 497 2509 2919
Size: 497 2012 410
Fragments arranged by size:
2012 497 410
PspOMI G'GGCC_C
Cuts at: 0 2815 2919
Size: 2815 104
PspXI vC'TCGA_Gb
Cuts at: 0 1697 2919
Size: 1697 1222
PstI C_TGCA'G
Cuts at: 0 489 2511 2919
Size: 489 2022 408
Fragments arranged by size:
2022 489 408
PvuII CAG'CTG
Cuts at: 0 1150 2228 2919
Size: 1150 1078 691
Fragments arranged by size:
1150 1078 691
SacI G_AGCT'C
Cuts at: 0 1698 1784 2919
Size: 1698 86 1135
Fragments arranged by size:
1698 1135 86
ScaI AGT'ACT
Cuts at: 0 2172 2919
Size: 2172 747
SfcI C'TryA_G
Cuts at: 0 305 485 2507 2919
Size: 305 180 2022 412
Fragments arranged by size:
2022 412 305 180
SfoI GGC'GCC
Cuts at: 0 2107 2919
Size: 2107 812
SmlI C'TyrA_G
Cuts at: 0 323 997 1697 1870 2919
Size: 323 674 700 173 1049
Fragments arranged by size:
1049 700 674 323 173
SphI G_CATG'C
Cuts at: 0 1147 1179 2919
Size: 1147 32 1740
Fragments arranged by size:
1740 1147 32
StuI AGG'CCT
Cuts at: 0 2896 2919
Size: 2896 23
StyI C'CwwG_G
Cuts at: 0 251 2919
Size: 251 2668
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 45 487 2587 2800 2919
Size: 45 442 2100 213 119
Fragments arranged by size:
2100 442 213 119 45
TatI w'GTAC_w
Cuts at: 0 2 145 2170 2870 2919
Size: 2 143 2025 700 49
Fragments arranged by size:
2025 700 143 49 2
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1376 1408 2522 2554 2633 2665 2919
Size: 1376 32 1114 32 79 32 254
Fragments arranged by size:
1376 1114 254 79 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 39 2683 2919
Size: 39 2644 236
Fragments arranged by size:
2644 236 39
XhoI C'TCGA_G
Cuts at: 0 1697 2919
Size: 1697 1222
XmnI GAAnn'nnTTC
Cuts at: 0 2632 2919
Size: 2632 287
Enzymes that do cut:
AccI AcuI AflII AflIII AleI AlwNI ApaI ApoI
AseI AvaI BanI BanII BbeI BbsI BcgI BclI
BglII Bme1580I BpmI BpuEI BsaI BsaAI BsaHI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI
BssSI BstXI BstYI BtgI BtgZI BtsI DraI DraIII
EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII KasI MmeI MscI
MslI MspA1I NarI NcoI NspI PasI PciI PpuMI
PshAI PspOMI PspXI PstI PvuII SacI ScaI SfcI
SfoI SmlI SphI StuI StyI TaqII TatI TstI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AgeI AhdI AlfI
AloI ApaLI AscI AsiSI AvrII BaeI BamHI BbvCI
BciVI BfrBI BglI BlpI BmgBI BmrI BmtI BplI
Bpu10I BsaBI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BsrFI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I ClaI CspCI DrdI EagI EcoNI FseI FspI
FspAI HpaI KpnI MfeI MluI NaeI NdeI NgoMIV
NheI NotI NruI NsiI PacI PflMI PfoI PmeI
PmlI PpiI PsiI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI SexAI SfiI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XmaI
ZraI