Restriction map for
pFN21AE6743
(Linear) (Six-base) MAPSORT of: KIEE6743.seq Check: 9459 from: 1 to: 1884
KIEE6743.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 136 259 1594 1884
Size: 136 123 1335 290
Fragments arranged by size:
1335 290 136 123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 847 940 946 1136 1644 1794 1884
Size: 847 93 6 190 508 150 90
Fragments arranged by size:
847 508 190 150 93 90 6
AfeI AGC'GCT
Cuts at: 0 1873 1884
Size: 1873 11
AflII C'TTAA_G
Cuts at: 0 1002 1884
Size: 1002 882
AleI CACnn'nnGTG
Cuts at: 0 1049 1060 1884
Size: 1049 11 824
Fragments arranged by size:
1049 824 11
AlwNI CAG_nnn'CTG
Cuts at: 0 898 1281 1461 1884
Size: 898 383 180 423
Fragments arranged by size:
898 423 383 180
ApaLI G'TGCA_C
Cuts at: 0 1124 1884
Size: 1124 760
ApoI r'AATT_y
Cuts at: 0 506 907 1884
Size: 506 401 977
Fragments arranged by size:
977 506 401
AvaI C'yCGr_G
Cuts at: 0 224 796 1884
Size: 224 572 1088
Fragments arranged by size:
1088 572 224
BanI G'GyrC_C
Cuts at: 0 809 1780 1884
Size: 809 971 104
Fragments arranged by size:
971 809 104
BanII G_rGCy'C
Cuts at: 0 1262 1884
Size: 1262 622
BbeI G_GCGC'C
Cuts at: 0 813 1784 1884
Size: 813 971 100
Fragments arranged by size:
971 813 100
BbsI GAAGACnn'nnnn_
Cuts at: 0 857 1884
Size: 857 1027
BbvCI CC'TCA_GC
Cuts at: 0 1166 1884
Size: 1166 718
BglI GCCn_nnn'nGGC
Cuts at: 0 491 1700 1884
Size: 491 1209 184
Fragments arranged by size:
1209 491 184
BlpI GC'TnA_GC
Cuts at: 0 1022 1884
Size: 1022 862
Bme1580I G_kGCm'C
Cuts at: 0 712 1128 1884
Size: 712 416 756
Fragments arranged by size:
756 712 416
BmgBI CAC'GTC
Cuts at: 0 1488 1884
Size: 1488 396
BmrI ACTGGGnnnn_n'
Cuts at: 0 1845 1854 1884
Size: 1845 9 30
Fragments arranged by size:
1845 30 9
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 676 682 697 1537 1562 1863 1884
Size: 251 425 6 15 840 25 301 21
Fragments arranged by size:
840 425 301 251 25 21 15 6
Bpu10I CC'TnA_GC
Cuts at: 0 1166 1677 1884
Size: 1166 511 207
Fragments arranged by size:
1166 511 207
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1354 1884
Size: 760 594 530
Fragments arranged by size:
760 594 530
BsaI GGTCTCn'nnnn_
Cuts at: 0 72 673 1884
Size: 72 601 1211
Fragments arranged by size:
1211 601 72
BsaAI yAC'GTr
Cuts at: 0 1447 1884
Size: 1447 437
BsaHI Gr'CG_yC
Cuts at: 0 810 1781 1884
Size: 810 971 103
Fragments arranged by size:
971 810 103
BsaWI w'CCGG_w
Cuts at: 0 134 1884
Size: 134 1750
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 27 57 243 273 670 700 1884
Size: 27 30 186 30 397 30 1184
Fragments arranged by size:
1184 397 186 30 30 30 27
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1540 1631 1884
Size: 1540 91 253
Fragments arranged by size:
1540 253 91
BseYI C'CCAG_C
Cuts at: 0 1868 1884
Size: 1868 16
BsiEI CG_ry'CG
Cuts at: 0 54 1884
Size: 54 1830
BsiHKAI G_wGCw'C
Cuts at: 0 1128 1262 1884
Size: 1128 134 622
Fragments arranged by size:
1128 622 134
BsmI GAATG_Cn'
Cuts at: 0 1320 1884
Size: 1320 564
Bsp1286I G_dGCh'C
Cuts at: 0 712 1128 1262 1884
Size: 712 416 134 622
Fragments arranged by size:
712 622 416 134
BspEI T'CCGG_A
Cuts at: 0 134 1884
Size: 134 1750
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 136 259 701 779 1331 1594 1884
Size: 136 123 442 78 552 263 290
Fragments arranged by size:
552 442 290 263 136 123 78
BsrBI CCG'CTC
Cuts at: 0 56 140 296 594 1884
Size: 56 84 156 298 1290
Fragments arranged by size:
1290 298 156 84 56
BsrDI GCAATG_nn'
Cuts at: 0 1109 1334 1884
Size: 1109 225 550
Fragments arranged by size:
1109 550 225
BstXI CCAn_nnnn'nTGG
Cuts at: 0 207 1884
Size: 207 1677
BtgI C'CryG_G
Cuts at: 0 1073 1205 1571 1884
Size: 1073 132 366 313
Fragments arranged by size:
1073 366 313 132
BtsI GCAGTG_nn'
Cuts at: 0 1689 1884
Size: 1689 195
EaeI y'GGCC_r
Cuts at: 0 51 1331 1692 1817 1884
Size: 51 1280 361 125 67
Fragments arranged by size:
1280 361 125 67 51
EagI C'GGCC_G
Cuts at: 0 51 1884
Size: 51 1833
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 131 264 1584 1643 1884
Size: 131 133 1320 59 241
Fragments arranged by size:
1320 241 133 131 59
EcoICRI GAG'CTC
Cuts at: 0 1260 1884
Size: 1260 624
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 251 676 682 697 847 940 946 1136
Size: 251 425 6 15 150 93 6 190
Cuts at: 1136 1537 1562 1644 1794 1863 1884
Size: 401 25 82 150 69 21
Fragments arranged by size:
425 401 251 190 150 150 93 82
69 25 21 15 6 6
EcoO109I rG'GnC_Cy
Cuts at: 0 122 840 1007 1170 1884
Size: 122 718 167 163 714
Fragments arranged by size:
718 714 167 163 122
EcoRI G'AATT_C
Cuts at: 0 506 1884
Size: 506 1378
EcoRV GAT'ATC
Cuts at: 0 1140 1884
Size: 1140 744
HaeII r_GCGC'y
Cuts at: 0 813 1784 1875 1884
Size: 813 971 91 9
Fragments arranged by size:
971 813 91 9
KasI G'GCGC_C
Cuts at: 0 809 1780 1884
Size: 809 971 104
Fragments arranged by size:
971 809 104
MslI CAynn'nnrTG
Cuts at: 0 205 1049 1060 1223 1570 1884
Size: 205 844 11 163 347 314
Fragments arranged by size:
844 347 314 205 163 11
MspA1I CmG'CkG
Cuts at: 0 214 257 895 1742 1751 1760 1884
Size: 214 43 638 847 9 9 124
Fragments arranged by size:
847 638 214 124 43 9 9
NarI GG'CG_CC
Cuts at: 0 810 1781 1884
Size: 810 971 103
Fragments arranged by size:
971 810 103
NcoI C'CATG_G
Cuts at: 0 1073 1884
Size: 1073 811
PasI CC'CwG_GG
Cuts at: 0 487 1884
Size: 487 1397
PfoI T'CCnGG_A
Cuts at: 0 653 674 1884
Size: 653 21 1210
Fragments arranged by size:
1210 653 21
PpuMI rG'GwC_Cy
Cuts at: 0 840 1007 1884
Size: 840 167 877
Fragments arranged by size:
877 840 167
PstI C_TGCA'G
Cuts at: 0 542 1202 1328 1403 1884
Size: 542 660 126 75 481
Fragments arranged by size:
660 542 481 126 75
PvuII CAG'CTG
Cuts at: 0 214 257 895 1884
Size: 214 43 638 989
Fragments arranged by size:
989 638 214 43
SacI G_AGCT'C
Cuts at: 0 1262 1884
Size: 1262 622
SexAI A'CCwGG_T
Cuts at: 0 842 1884
Size: 842 1042
SfcI C'TryA_G
Cuts at: 0 538 1198 1324 1399 1411 1884
Size: 538 660 126 75 12 473
Fragments arranged by size:
660 538 473 126 75 12
SfoI GGC'GCC
Cuts at: 0 811 1782 1884
Size: 811 971 102
Fragments arranged by size:
971 811 102
SmaI CCC'GGG
Cuts at: 0 226 1884
Size: 226 1658
SmlI C'TyrA_G
Cuts at: 0 739 1002 1369 1884
Size: 739 263 367 515
Fragments arranged by size:
739 515 367 263
StyI C'CwwG_G
Cuts at: 0 1073 1884
Size: 1073 811
TatI w'GTAC_w
Cuts at: 0 1529 1884
Size: 1529 355
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 75 107 243 275 670 702 1884
Size: 75 32 136 32 395 32 1182
Fragments arranged by size:
1182 395 136 75 32 32 32
XmaI C'CCGG_G
Cuts at: 0 224 1884
Size: 224 1660
Enzymes that do cut:
AarI AcuI AfeI AflII AleI AlwNI ApaLI ApoI
AvaI BanI BanII BbeI BbsI BbvCI BglI BlpI
Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspMI BsrBI BsrDI BstXI BtgI BtsI
EaeI EagI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV
HaeII KasI MslI MspA1I NarI NcoI PasI PfoI
PpuMI PstI PvuII SacI SexAI SfcI SfoI SmaI
SmlI StyI TatI TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflIII AgeI AhdI AlfI
AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI
BcgI BciVI BclI BfrBI BglII BmtI BplI BsaBI
BsgI BsiWI BsmBI BspHI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EarI EcoNI FalI FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XcmI XhoI XmnI ZraI