Restriction map for
pFN21AE6740
(Linear) (Six-base) MAPSORT of: KIEE6740.seq Check: 8146 from: 1 to: 2328
KIEE6740.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1086 2104 2328
Size: 1086 1018 224
Fragments arranged by size:
1086 1018 224
AatII G_ACGT'C
Cuts at: 0 1664 2328
Size: 1664 664
AccI GT'mk_AC
Cuts at: 0 1412 2328
Size: 1412 916
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 2219 2328
Size: 80 2139 109
Fragments arranged by size:
2139 109 80
AflIII A'CryG_T
Cuts at: 0 1631 1709 2328
Size: 1631 78 619
Fragments arranged by size:
1631 619 78
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 862 894 2328
Size: 862 32 1434
Fragments arranged by size:
1434 862 32
AlwNI CAG_nnn'CTG
Cuts at: 0 282 567 2328
Size: 282 285 1761
Fragments arranged by size:
1761 285 282
ApaI G_GGCC'C
Cuts at: 0 322 2328
Size: 322 2006
ApoI r'AATT_y
Cuts at: 0 1975 2328
Size: 1975 353
AvaI C'yCGr_G
Cuts at: 0 1301 2328
Size: 1301 1027
BanI G'GyrC_C
Cuts at: 0 1443 2328
Size: 1443 885
BanII G_rGCy'C
Cuts at: 0 322 1651 2276 2328
Size: 322 1329 625 52
Fragments arranged by size:
1329 625 322 52
BbsI GAAGACnn'nnnn_
Cuts at: 0 650 2328
Size: 650 1678
BciVI GTATCCnnnnn_n'
Cuts at: 0 579 2328
Size: 579 1749
BglI GCCn_nnn'nGGC
Cuts at: 0 337 2328
Size: 337 1991
BlpI GC'TnA_GC
Cuts at: 0 2089 2275 2328
Size: 2089 186 53
Fragments arranged by size:
2089 186 53
Bme1580I G_kGCm'C
Cuts at: 0 322 1448 2328
Size: 322 1126 880
Fragments arranged by size:
1126 880 322
BmrI ACTGGGnnnn_n'
Cuts at: 0 270 555 2328
Size: 270 285 1773
Fragments arranged by size:
1773 285 270
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 389 760 799 1330 1525 2328
Size: 389 371 39 531 195 803
Fragments arranged by size:
803 531 389 371 195 39
Bpu10I CC'TnA_GC
Cuts at: 0 1759 2328
Size: 1759 569
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1846 2060 2328
Size: 1846 214 268
Fragments arranged by size:
1846 268 214
BsaI GGTCTCn'nnnn_
Cuts at: 0 229 775 2251 2328
Size: 229 546 1476 77
Fragments arranged by size:
1476 546 229 77
BsaHI Gr'CG_yC
Cuts at: 0 1661 2328
Size: 1661 667
BsaWI w'CCGG_w
Cuts at: 0 597 1872 2237 2328
Size: 597 1275 365 91
Fragments arranged by size:
1275 597 365 91
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 82 112 579 609 1306 1336 2328
Size: 82 30 467 30 697 30 992
Fragments arranged by size:
992 697 467 82 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 617 1280 1327 1384 1849 1913 2328
Size: 617 663 47 57 465 64 415
Fragments arranged by size:
663 617 465 415 64 57 47
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 1096 2328
Size: 575 521 1232
Fragments arranged by size:
1232 575 521
BsiEI CG_ry'CG
Cuts at: 0 989 1359 1780 2328
Size: 989 370 421 548
Fragments arranged by size:
989 548 421 370
BsiHKAI G_wGCw'C
Cuts at: 0 113 746 1733 1765 2095 2276 2328
Size: 113 633 987 32 330 181 52
Fragments arranged by size:
987 633 330 181 113 52 32
BsmI GAATG_Cn'
Cuts at: 0 2245 2328
Size: 2245 83
Bsp1286I G_dGCh'C
Cuts at: 0 113 322 746 1448 1651 1733 1765 2095
Size: 113 209 424 702 203 82 32 330
Cuts at: 2095 2276 2328
Size: 181 52
Fragments arranged by size:
702 424 330 209 203 181 113 82
52 32
BspEI T'CCGG_A
Cuts at: 0 597 2237 2328
Size: 597 1640 91
Fragments arranged by size:
1640 597 91
BspHI T'CATG_A
Cuts at: 0 888 2328
Size: 888 1440
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1086 2104 2328
Size: 1086 1018 224
Fragments arranged by size:
1086 1018 224
BsrBI CCG'CTC
Cuts at: 0 2138 2328
Size: 2138 190
BsrDI GCAATG_nn'
Cuts at: 0 1910 2066 2274 2328
Size: 1910 156 208 54
Fragments arranged by size:
1910 208 156 54
BsrFI r'CCGG_y
Cuts at: 0 851 1163 1795 2328
Size: 851 312 632 533
Fragments arranged by size:
851 632 533 312
BstAPI GCAn_nnn'nTGC
Cuts at: 0 400 1014 2328
Size: 400 614 1314
Fragments arranged by size:
1314 614 400
BstBI TT'CG_AA
Cuts at: 0 2214 2328
Size: 2214 114
BstEII G'GTnAC_C
Cuts at: 0 423 2328
Size: 423 1905
BstXI CCAn_nnnn'nTGG
Cuts at: 0 489 1725 2328
Size: 489 1236 603
Fragments arranged by size:
1236 603 489
BstYI r'GATC_y
Cuts at: 0 1904 2234 2328
Size: 1904 330 94
Fragments arranged by size:
1904 330 94
Bsu36I CC'TnA_GG
Cuts at: 0 1367 2328
Size: 1367 961
BtgI C'CryG_G
Cuts at: 0 31 547 577 1339 1848 2328
Size: 31 516 30 762 509 480
Fragments arranged by size:
762 516 509 480 31 30
BtsI GCAGTG_nn'
Cuts at: 0 631 1829 2328
Size: 631 1198 499
Fragments arranged by size:
1198 631 499
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 341 376 2328
Size: 341 35 1952
Fragments arranged by size:
1952 341 35
DraIII CAC_nnn'GTG
Cuts at: 0 1815 2328
Size: 1815 513
EaeI y'GGCC_r
Cuts at: 0 338 491 986 1356 1777 2328
Size: 338 153 495 370 421 551
Fragments arranged by size:
551 495 421 370 338 153
EagI C'GGCC_G
Cuts at: 0 986 1356 1777 2328
Size: 986 370 421 551
Fragments arranged by size:
986 551 421 370
EarI CTCTTCn'nnn_
Cuts at: 0 113 596 922 1576 1861 2300 2328
Size: 113 483 326 654 285 439 28
Fragments arranged by size:
654 483 439 326 285 113 28
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 103 598 2328
Size: 103 495 1730
Fragments arranged by size:
1730 495 103
EcoICRI GAG'CTC
Cuts at: 0 2274 2328
Size: 2274 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 389 760 799 1330 1525 2219 2328
Size: 80 309 371 39 531 195 694 109
Fragments arranged by size:
694 531 371 309 195 109 80 39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1093 1528 1984 2328
Size: 1093 435 456 344
Fragments arranged by size:
1093 456 435 344
EcoO109I rG'GnC_Cy
Cuts at: 0 318 319 498 606 2194 2328
Size: 318 1 179 108 1588 134
Fragments arranged by size:
1588 318 179 134 108 1
EcoRI G'AATT_C
Cuts at: 0 1975 2328
Size: 1975 353
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 625 2328
Size: 625 1703
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 124 156 211 243 1597 1629 2328
Size: 124 32 55 32 1354 32 699
Fragments arranged by size:
1354 699 124 55 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1664 2328
Size: 1664 664
MscI TGG'CCA
Cuts at: 0 493 2328
Size: 493 1835
MslI CAynn'nnrTG
Cuts at: 0 980 1441 1726 1915 2328
Size: 980 461 285 189 413
Fragments arranged by size:
980 461 413 285 189
MspA1I CmG'CkG
Cuts at: 0 22 33 420 434 549 579 2328
Size: 22 11 387 14 115 30 1749
Fragments arranged by size:
1749 387 115 30 22 14 11
NaeI GCC'GGC
Cuts at: 0 1165 1797 2328
Size: 1165 632 531
Fragments arranged by size:
1165 632 531
NgoMIV G'CCGG_C
Cuts at: 0 1163 1795 2328
Size: 1163 632 533
Fragments arranged by size:
1163 632 533
NotI GC'GGCC_GC
Cuts at: 0 986 2328
Size: 986 1342
NspI r_CATG'y
Cuts at: 0 699 1428 1635 1713 1999 2328
Size: 699 729 207 78 286 329
Fragments arranged by size:
729 699 329 286 207 78
PciI A'CATG_T
Cuts at: 0 1631 1709 2328
Size: 1631 78 619
Fragments arranged by size:
1631 619 78
PflMI CCAn_nnn'nTGG
Cuts at: 0 567 810 1492 2328
Size: 567 243 682 836
Fragments arranged by size:
836 682 567 243
PfoI T'CCnGG_A
Cuts at: 0 509 2328
Size: 509 1819
PpuMI rG'GwC_Cy
Cuts at: 0 2194 2328
Size: 2194 134
PsiI TTA'TAA
Cuts at: 0 2186 2328
Size: 2186 142
PspOMI G'GGCC_C
Cuts at: 0 318 2328
Size: 318 2010
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1672 1704 2328
Size: 1672 32 624
Fragments arranged by size:
1672 624 32
PstI C_TGCA'G
Cuts at: 0 206 2135 2328
Size: 206 1929 193
Fragments arranged by size:
1929 206 193
SacI G_AGCT'C
Cuts at: 0 2276 2328
Size: 2276 52
SacII CC_GC'GG
Cuts at: 0 34 550 580 2328
Size: 34 516 30 1748
Fragments arranged by size:
1748 516 34 30
ScaI AGT'ACT
Cuts at: 0 1273 1330 2328
Size: 1273 57 998
Fragments arranged by size:
1273 998 57
SexAI A'CCwGG_T
Cuts at: 0 1811 2328
Size: 1811 517
SfcI C'TryA_G
Cuts at: 0 202 1689 2131 2328
Size: 202 1487 442 197
Fragments arranged by size:
1487 442 202 197
SgrAI Cr'CCGG_yG
Cuts at: 0 851 2328
Size: 851 1477
SmaI CCC'GGG
Cuts at: 0 1303 2328
Size: 1303 1025
SmlI C'TyrA_G
Cuts at: 0 1861 2075 2328
Size: 1861 214 253
Fragments arranged by size:
1861 253 214
SspI AAT'ATT
Cuts at: 0 1954 2328
Size: 1954 374
StyI C'CwwG_G
Cuts at: 0 188 494 2328
Size: 188 306 1834
Fragments arranged by size:
1834 306 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 260 1822 2328
Size: 260 1562 506
Fragments arranged by size:
1562 506 260
TatI w'GTAC_w
Cuts at: 0 1271 1328 1703 2328
Size: 1271 57 375 625
Fragments arranged by size:
1271 625 375 57
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1136 1168 1621 1653 2328
Size: 1136 32 453 32 675
Fragments arranged by size:
1136 675 453 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 308 357 1809 2328
Size: 308 49 1452 519
Fragments arranged by size:
1452 519 308 49
XmaI C'CCGG_G
Cuts at: 0 1301 2328
Size: 1301 1027
XmnI GAAnn'nnTTC
Cuts at: 0 256 1513 2328
Size: 256 1257 815
Fragments arranged by size:
1257 815 256
ZraI GAC'GTC
Cuts at: 0 1662 2328
Size: 1662 666
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AloI AlwNI ApaI
ApoI AvaI BanI BanII BbsI BciVI BglI BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaHI BsaWI
BsaXI BseRI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtsI CspCI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI Hin4I MmeI MscI MslI MspA1I NaeI NgoMIV
NotI NspI PciI PflMI PfoI PpuMI PsiI PspOMI
PsrI PstI SacI SacII ScaI SexAI SfcI SgrAI
SmaI SmlI SspI StyI TaqII TatI TstI XcmI
XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI AleI AlfI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BcgI BclI BfrBI BglII BmgBI BmtI BplI
BsaAI BsaBI BseYI BsiWI BsmBI BsrGI BssHII BssSI
BstZ17I BtgZI ClaI DraI DrdI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI NarI NcoI NdeI NheI NruI NsiI PacI
PasI PmeI PmlI PpiI PshAI PspXI PvuI PvuII
RsrII SalI SanDI SapI SbfI SfiI SfoI SgfI
SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI
XhoI