Restriction map for
pFN21AE6736
(Linear) (Six-base) MAPSORT of: KIEE6736.seq Check: 8381 from: 1 to: 4725
KIEE6736.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 797 1207 1832 2818 2885 3209 4317 4725
Size: 797 410 625 986 67 324 1108 408
Fragments arranged by size:
1108 986 797 625 410 408 324 67
AatII G_ACGT'C
Cuts at: 0 3185 4725
Size: 3185 1540
AccI GT'mk_AC
Cuts at: 0 3185 4725
Size: 3185 1540
Acc65I G'GTAC_C
Cuts at: 0 3629 4725
Size: 3629 1096
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 3100 3710 3815 3829 4361 4725
Size: 424 2676 610 105 14 532 364
Fragments arranged by size:
2676 610 532 424 364 105 14
AfeI AGC'GCT
Cuts at: 0 100 226 907 1096 4725
Size: 100 126 681 189 3629
Fragments arranged by size:
3629 681 189 126 100
AflIII A'CryG_T
Cuts at: 0 155 725 4725
Size: 155 570 4000
Fragments arranged by size:
4000 570 155
AhdI GACnn_n'nnGTC
Cuts at: 0 1155 2478 4725
Size: 1155 1323 2247
Fragments arranged by size:
2247 1323 1155
AleI CACnn'nnGTG
Cuts at: 0 394 1064 2947 4271 4725
Size: 394 670 1883 1324 454
Fragments arranged by size:
1883 1324 670 454 394
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2601 2706 3413 4289 4725
Size: 2601 105 707 876 436
Fragments arranged by size:
2601 876 707 436 105
AlwNI CAG_nnn'CTG
Cuts at: 0 534 1286 1436 2699 3137 3288 3526 3991
Size: 534 752 150 1263 438 151 238 465
Cuts at: 3991 4332 4359 4512 4620 4677 4725
Size: 341 27 153 108 57 48
Fragments arranged by size:
1263 752 534 465 438 341 238 153
151 150 108 57 48 27
ApaI G_GGCC'C
Cuts at: 0 942 2191 3540 4725
Size: 942 1249 1349 1185
Fragments arranged by size:
1349 1249 1185 942
ApaLI G'TGCA_C
Cuts at: 0 2805 4725
Size: 2805 1920
ApoI r'AATT_y
Cuts at: 0 1107 4725
Size: 1107 3618
AvaI C'yCGr_G
Cuts at: 0 172 520 941 1249 1389 1696 2334 3215
Size: 172 348 421 308 140 307 638 881
Cuts at: 3215 3247 3269 3765 3788 4563 4702 4725
Size: 32 22 496 23 775 139 23
Fragments arranged by size:
881 775 638 496 421 348 308 307
172 140 139 32 23 23 22
AvrII C'CTAG_G
Cuts at: 0 369 4725
Size: 369 4356
BamHI G'GATC_C
Cuts at: 0 3319 4725
Size: 3319 1406
BanI G'GyrC_C
Cuts at: 0 39 89 196 872 946 1195 1689 2137
Size: 39 50 107 676 74 249 494 448
Cuts at: 2137 2405 2846 3298 3629 4725
Size: 268 441 452 331 1096
Fragments arranged by size:
1096 676 494 452 448 441 331 268
249 107 74 50 39
BanII G_rGCy'C
Cuts at: 0 216 316 476 667 942 1025 1555 1579
Size: 216 100 160 191 275 83 530 24
Cuts at: 1579 1600 1619 1814 2191 2399 2617 2770 3216
Size: 21 19 195 377 208 218 153 446
Cuts at: 3216 3540 3774 4561 4725
Size: 324 234 787 164
Fragments arranged by size:
787 530 446 377 324 275 234 218
216 208 195 191 164 160 153 100
83 24 21 19
BbeI G_GCGC'C
Cuts at: 0 93 200 4725
Size: 93 107 4525
Fragments arranged by size:
4525 107 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 511 1610 3334 3803 3835 4132 4725
Size: 511 1099 1724 469 32 297 593
Fragments arranged by size:
1724 1099 593 511 469 297 32
BbvCI CC'TCA_GC
Cuts at: 0 1501 1723 4246 4725
Size: 1501 222 2523 479
Fragments arranged by size:
2523 1501 479 222
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2214 2248 4693 4725
Size: 2214 34 2445 32
Fragments arranged by size:
2445 2214 34 32
BciVI GTATCCnnnnn_n'
Cuts at: 0 2062 4725
Size: 2062 2663
BfrBI ATG'CAT
Cuts at: 0 2588 4725
Size: 2588 2137
BglI GCCn_nnn'nGGC
Cuts at: 0 1775 2336 3472 3757 3862 4725
Size: 1775 561 1136 285 105 863
Fragments arranged by size:
1775 1136 863 561 285 105
BlpI GC'TnA_GC
Cuts at: 0 4370 4390 4725
Size: 4370 20 335
Fragments arranged by size:
4370 335 20
Bme1580I G_kGCm'C
Cuts at: 0 942 949 1694 2191 2408 2809 3303 3312
Size: 942 7 745 497 217 401 494 9
Cuts at: 3312 3483 3540 4533 4725
Size: 171 57 993 192
Fragments arranged by size:
993 942 745 497 494 401 217 192
171 57 9 7
BmgBI CAC'GTC
Cuts at: 0 849 1845 4725
Size: 849 996 2880
Fragments arranged by size:
2880 996 849
BmrI ACTGGGnnnn_n'
Cuts at: 0 1128 1473 2638 2773 2917 2919 3431 4725
Size: 1128 345 1165 135 144 2 512 1294
Fragments arranged by size:
1294 1165 1128 512 345 144 135 2
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 748 1162 2707 3250 3373 3385 3436
Size: 649 99 414 1545 543 123 12 51
Cuts at: 3436 3604 3640 3844 3862 4158 4381 4453 4615
Size: 168 36 204 18 296 223 72 162
Cuts at: 4615 4699 4725
Size: 84 26
Fragments arranged by size:
1545 649 543 414 296 223 204 168
162 123 99 84 72 51 36 26
18 12
Bpu10I CC'TnA_GC
Cuts at: 0 1501 1594 1723 1813 4246 4725
Size: 1501 93 129 90 2433 479
Fragments arranged by size:
2433 1501 479 129 93 90
BsaI GGTCTCn'nnnn_
Cuts at: 0 1637 1825 3598 4441 4725
Size: 1637 188 1773 843 284
Fragments arranged by size:
1773 1637 843 284 188
BsaAI yAC'GTr
Cuts at: 0 158 192 392 4725
Size: 158 34 200 4333
Fragments arranged by size:
4333 200 158 34
BsaHI Gr'CG_yC
Cuts at: 0 90 197 233 3182 4318 4336 4725
Size: 90 107 36 2949 1136 18 389
Fragments arranged by size:
2949 1136 389 107 90 36 18
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 247 277 478 508 2302 2332 2767 2797
Size: 247 30 201 30 1794 30 435 30
Cuts at: 2797 3613 3643 4725
Size: 816 30 1082
Fragments arranged by size:
1794 1082 816 435 247 201 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 673 691 1624 1627 1873 3079 3394 4725
Size: 673 18 933 3 246 1206 315 1331
Fragments arranged by size:
1331 1206 933 673 315 246 18 3
BseYI C'CCAG_C
Cuts at: 0 21 245 640 1078 1927 2391 2411 2853
Size: 21 224 395 438 849 464 20 442
Cuts at: 2853 3159 3338 3608 4149 4322 4384 4473 4500
Size: 306 179 270 541 173 62 89 27
Cuts at: 4500 4554 4607 4725
Size: 54 53 118
Fragments arranged by size:
849 541 464 442 438 395 306 270
224 179 173 118 89 62 54 53
27 21 20
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 355 552 1272 2103 2488 2666 2973 3318
Size: 355 197 720 831 385 178 307 345
Cuts at: 3318 3736 3913 4675 4725
Size: 418 177 762 50
Fragments arranged by size:
831 762 720 418 385 355 345 307
197 178 177 50
BsiEI CG_ry'CG
Cuts at: 0 53 516 4725
Size: 53 463 4209
Fragments arranged by size:
4209 463 53
BsiHKAI G_wGCw'C
Cuts at: 0 301 764 1025 1562 1619 1814 2064 2534
Size: 301 463 261 537 57 195 250 470
Cuts at: 2534 2809 2902 3774 4725
Size: 275 93 872 951
Fragments arranged by size:
951 872 537 470 463 301 275 261
250 195 93 57
BsmI GAATG_Cn'
Cuts at: 0 1009 4725
Size: 1009 3716
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3416 4725
Size: 3416 1309
Bsp1286I G_dGCh'C
Cuts at: 0 216 301 316 476 667 764 942 949
Size: 216 85 15 160 191 97 178 7
Cuts at: 949 1025 1555 1562 1579 1600 1619 1694 1814
Size: 76 530 7 17 21 19 75 120
Cuts at: 1814 2064 2191 2399 2408 2534 2617 2770 2809
Size: 250 127 208 9 126 83 153 39
Cuts at: 2809 2902 3216 3303 3312 3483 3540 3774 4533
Size: 93 314 87 9 171 57 234 759
Cuts at: 4533 4561 4725
Size: 28 164
Fragments arranged by size:
759 530 314 250 234 216 208 191
178 171 164 160 153 127 126 120
97 93 87 85 83 76 75 57
39 28 21 19 17 15 9 9
7 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 542 797 1207 1412 1832 2818 2885 3209
Size: 542 255 410 205 420 986 67 324
Cuts at: 3209 3340 4317 4725
Size: 131 977 408
Fragments arranged by size:
986 977 542 420 410 408 324 255
205 131 67
BsrBI CCG'CTC
Cuts at: 0 995 4254 4374 4725
Size: 995 3259 120 351
Fragments arranged by size:
3259 995 351 120
BsrDI GCAATG_nn'
Cuts at: 0 216 3040 4069 4725
Size: 216 2824 1029 656
Fragments arranged by size:
2824 1029 656 216
BsrFI r'CCGG_y
Cuts at: 0 52 108 515 635 716 869 889 1936
Size: 52 56 407 120 81 153 20 1047
Cuts at: 1936 4725
Size: 2789
Fragments arranged by size:
2789 1047 407 153 120 81 56 52
20
BsrGI T'GTAC_A
Cuts at: 0 1716 4725
Size: 1716 3009
BssSI C'ACGA_G
Cuts at: 0 964 2932 4116 4725
Size: 964 1968 1184 609
Fragments arranged by size:
1968 1184 964 609
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1286 2594 2699 3026 3526 4620 4698 4725
Size: 1286 1308 105 327 500 1094 78 27
Fragments arranged by size:
1308 1286 1094 500 327 105 78 27
BstEII G'GTnAC_C
Cuts at: 0 684 2518 4725
Size: 684 1834 2207
Fragments arranged by size:
2207 1834 684
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1078 1545 2003 2460 4725
Size: 1078 467 458 457 2265
Fragments arranged by size:
2265 1078 467 458 457
BstYI r'GATC_y
Cuts at: 0 2159 3319 4409 4625 4655 4725
Size: 2159 1160 1090 216 30 70
Fragments arranged by size:
2159 1160 1090 216 70 30
Bsu36I CC'TnA_GG
Cuts at: 0 1756 3425 4543 4725
Size: 1756 1669 1118 182
Fragments arranged by size:
1756 1669 1118 182
BtgI C'CryG_G
Cuts at: 0 43 200 320 466 934 1041 1126 1214
Size: 43 157 120 146 468 107 85 88
Cuts at: 1214 2459 2513 4725
Size: 1245 54 2212
Fragments arranged by size:
2212 1245 468 157 146 120 107 88
85 54 43
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1199 4725
Size: 1199 3526
BtsI GCAGTG_nn'
Cuts at: 0 993 1059 1338 1472 1855 2325 2487 3024
Size: 993 66 279 134 383 470 162 537
Cuts at: 3024 3365 3524 4725
Size: 341 159 1201
Fragments arranged by size:
1201 993 537 470 383 341 279 162
159 134 66
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 969 1004 4725
Size: 969 35 3721
Fragments arranged by size:
3721 969 35
DraIII CAC_nnn'GTG
Cuts at: 0 305 1090 4725
Size: 305 785 3635
Fragments arranged by size:
3635 785 305
EaeI y'GGCC_r
Cuts at: 0 50 513 990 1449 1978 2274 2484 3464
Size: 50 463 477 459 529 296 210 980
Cuts at: 3464 3707 3854 4211 4725
Size: 243 147 357 514
Fragments arranged by size:
980 529 514 477 463 459 357 296
243 210 147 50
EagI C'GGCC_G
Cuts at: 0 50 513 4725
Size: 50 463 4212
Fragments arranged by size:
4212 463 50
EarI CTCTTCn'nnn_
Cuts at: 0 1030 3371 4725
Size: 1030 2341 1354
Fragments arranged by size:
2341 1354 1030
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 355 1894 1962 4153 4725
Size: 355 1539 68 2191 572
Fragments arranged by size:
2191 1539 572 355 68
EcoICRI GAG'CTC
Cuts at: 0 1023 1617 1812 3772 4725
Size: 1023 594 195 1960 953
Fragments arranged by size:
1960 1023 953 594 195
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 649 748 1162 2707 3100 3250 3373
Size: 424 225 99 414 1545 393 150 123
Cuts at: 3373 3385 3436 3604 3640 3710 3815 3829 3844
Size: 12 51 168 36 70 105 14 15
Cuts at: 3844 3862 4158 4361 4381 4453 4615 4699 4725
Size: 18 296 203 20 72 162 84 26
Fragments arranged by size:
1545 424 414 393 296 225 203 168
162 150 123 105 99 84 72 70
51 36 26 20 18 15 14 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 373 1954 2841 3058 3428 3955 4245 4575
Size: 373 1581 887 217 370 527 290 330
Cuts at: 4575 4725
Size: 150
Fragments arranged by size:
1581 887 527 373 370 330 290 217
150
EcoO109I rG'GnC_Cy
Cuts at: 0 750 1122 1394 1587 2095 2187 2385 3168
Size: 750 372 272 193 508 92 198 783
Cuts at: 3168 3433 3536 3537 3615 3819 4509 4568 4725
Size: 265 103 1 78 204 690 59 157
Fragments arranged by size:
783 750 690 508 372 272 265 204
198 193 157 103 92 78 59 1
FseI GG_CCGG'CC
Cuts at: 0 519 639 4725
Size: 519 120 4086
Fragments arranged by size:
4086 519 120
HaeII r_GCGC'y
Cuts at: 0 34 93 102 200 228 252 494 647
Size: 34 59 9 98 28 24 242 153
Cuts at: 647 909 1053 1098 1341 2355 2561 2915 4725
Size: 262 144 45 243 1014 206 354 1810
Fragments arranged by size:
1810 1014 354 262 243 242 206 153
144 98 59 45 34 28 24 9
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1141 1173 2176 2208 2464 2496 4438 4470
Size: 1141 32 1003 32 256 32 1942 32
Cuts at: 4470 4588 4620 4725
Size: 118 32 105
Fragments arranged by size:
1942 1141 1003 256 118 105 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 354 4725
Size: 354 4371
HindIII A'AGCT_T
Cuts at: 0 4228 4725
Size: 4228 497
KasI G'GCGC_C
Cuts at: 0 89 196 4725
Size: 89 107 4529
Fragments arranged by size:
4529 107 89
KpnI G_GTAC'C
Cuts at: 0 3633 4725
Size: 3633 1092
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 812 3146 3575 4335 4725
Size: 812 2334 429 760 390
Fragments arranged by size:
2334 812 760 429 390
MscI TGG'CCA
Cuts at: 0 1451 2276 2486 3466 3709 3856 4213 4725
Size: 1451 825 210 980 243 147 357 512
Fragments arranged by size:
1451 980 825 512 357 243 210 147
MslI CAynn'nnrTG
Cuts at: 0 394 1064 2947 2949 4271 4725
Size: 394 670 1883 2 1322 454
Fragments arranged by size:
1883 1322 670 454 394 2
MspA1I CmG'CkG
Cuts at: 0 45 202 597 1078 1128 1241 1303 1423
Size: 45 157 395 481 50 113 62 120
Cuts at: 1423 1436 1650 1722 1878 2108 2391 2600 2699
Size: 13 214 72 156 230 283 209 99
Cuts at: 2699 2853 2987 3279 3305 3342 3397 3612 4554
Size: 154 134 292 26 37 55 215 942
Cuts at: 4554 4718 4725
Size: 164 7
Fragments arranged by size:
942 481 395 292 283 230 215 214
209 164 157 156 154 134 120 113
99 72 62 55 50 45 37 26
13 7
NaeI GCC'GGC
Cuts at: 0 54 517 637 4725
Size: 54 463 120 4088
Fragments arranged by size:
4088 463 120 54
NarI GG'CG_CC
Cuts at: 0 90 197 4725
Size: 90 107 4528
Fragments arranged by size:
4528 107 90
NcoI C'CATG_G
Cuts at: 0 320 466 4725
Size: 320 146 4259
Fragments arranged by size:
4259 320 146
NgoMIV G'CCGG_C
Cuts at: 0 52 515 635 4725
Size: 52 463 120 4090
Fragments arranged by size:
4090 463 120 52
NsiI A_TGCA'T
Cuts at: 0 2590 4725
Size: 2590 2135
NspI r_CATG'y
Cuts at: 0 829 2592 2948 3394 4725
Size: 829 1763 356 446 1331
Fragments arranged by size:
1763 1331 829 446 356
PasI CC'CwG_GG
Cuts at: 0 667 1799 3137 4725
Size: 667 1132 1338 1588
Fragments arranged by size:
1588 1338 1132 667
PflMI CCAn_nnn'nTGG
Cuts at: 0 3530 4725
Size: 3530 1195
PfoI T'CCnGG_A
Cuts at: 0 923 2064 3227 4412 4725
Size: 923 1141 1163 1185 313
Fragments arranged by size:
1185 1163 1141 923 313
PmlI CAC'GTG
Cuts at: 0 158 192 4725
Size: 158 34 4533
Fragments arranged by size:
4533 158 34
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1801 1833 4725
Size: 1801 32 2892
Fragments arranged by size:
2892 1801 32
PpuMI rG'GwC_Cy
Cuts at: 0 750 1122 1394 1587 2095 2385 3433 3615
Size: 750 372 272 193 508 290 1048 182
Cuts at: 3615 4568 4725
Size: 953 157
Fragments arranged by size:
1048 953 750 508 372 290 272 193
182 157
PshAI GACnn'nnGTC
Cuts at: 0 182 1241 1382 1957 4725
Size: 182 1059 141 575 2768
Fragments arranged by size:
2768 1059 575 182 141
PspOMI G'GGCC_C
Cuts at: 0 938 2187 3536 4725
Size: 938 1249 1349 1189
Fragments arranged by size:
1349 1249 1189 938
PspXI vC'TCGA_Gb
Cuts at: 0 3247 4725
Size: 3247 1478
PstI C_TGCA'G
Cuts at: 0 677 988 1282 1409 1432 1829 2173 2695
Size: 677 311 294 127 23 397 344 522
Cuts at: 2695 2896 3284 4043 4553 4625 4725
Size: 201 388 759 510 72 100
Fragments arranged by size:
759 677 522 510 397 388 344 311
294 201 127 100 72 23
PvuII CAG'CTG
Cuts at: 0 1078 1423 1436 1722 2108 2600 2853 3279
Size: 1078 345 13 286 386 492 253 426
Cuts at: 3279 3342 3397 4554 4718 4725
Size: 63 55 1157 164 7
Fragments arranged by size:
1157 1078 492 426 386 345 286 253
164 63 55 13 7
SacI G_AGCT'C
Cuts at: 0 1025 1619 1814 3774 4725
Size: 1025 594 195 1960 951
Fragments arranged by size:
1960 1025 951 594 195
SacII CC_GC'GG
Cuts at: 0 46 203 1129 4725
Size: 46 157 926 3596
Fragments arranged by size:
3596 926 157 46
SanDI GG'GwC_CC
Cuts at: 0 1122 2095 2385 4725
Size: 1122 973 290 2340
Fragments arranged by size:
2340 1122 973 290
SapI GCTCTTCn'nnn_
Cuts at: 0 1030 4725
Size: 1030 3695
SbfI CC_TGCA'GG
Cuts at: 0 1829 2896 4725
Size: 1829 1067 1829
Fragments arranged by size:
1829 1829 1067
SexAI A'CCwGG_T
Cuts at: 0 301 4725
Size: 301 4424
SfcI C'TryA_G
Cuts at: 0 486 673 984 1278 1405 1428 1825 2169
Size: 486 187 311 294 127 23 397 344
Cuts at: 2169 2691 2892 3280 4039 4549 4621 4725
Size: 522 201 388 759 510 72 104
Fragments arranged by size:
759 522 510 486 397 388 344 311
294 201 187 127 104 72 23
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 3472 4725
Size: 3472 1253
SfoI GGC'GCC
Cuts at: 0 91 198 4725
Size: 91 107 4527
Fragments arranged by size:
4527 107 91
SmaI CCC'GGG
Cuts at: 0 943 1251 1391 1698 2336 3271 3767 3790
Size: 943 308 140 307 638 935 496 23
Cuts at: 3790 4565 4725
Size: 775 160
Fragments arranged by size:
943 935 775 638 496 308 307 160
140 23
SmlI C'TyrA_G
Cuts at: 0 3247 4725
Size: 3247 1478
SphI G_CATG'C
Cuts at: 0 829 2592 3394 4725
Size: 829 1763 802 1331
Fragments arranged by size:
1763 1331 829 802
StuI AGG'CCT
Cuts at: 0 148 2836 2958 4240 4725
Size: 148 2688 122 1282 485
Fragments arranged by size:
2688 1282 485 148 122
StyI C'CwwG_G
Cuts at: 0 320 369 466 755 1457 3114 3172 4055
Size: 320 49 97 289 702 1657 58 883
Cuts at: 4055 4214 4235 4725
Size: 159 21 490
Fragments arranged by size:
1657 883 702 490 320 289 159 97
58 49 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 339 1995 2607 2621 2774 3352 4369 4504
Size: 339 1656 612 14 153 578 1017 135
Cuts at: 4504 4725
Size: 221
Fragments arranged by size:
1656 1017 612 578 339 221 153 135
14
TatI w'GTAC_w
Cuts at: 0 1716 4725
Size: 1716 3009
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 247 279 919 951 1281 1313 2302 2334
Size: 247 32 640 32 330 32 989 32
Cuts at: 2334 2767 2799 4725
Size: 433 32 1926
Fragments arranged by size:
1926 989 640 433 330 247 32 32
32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 4090 4452 4725
Size: 4090 362 273
Fragments arranged by size:
4090 362 273
XbaI T'CTAG_A
Cuts at: 0 711 4725
Size: 711 4014
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1420 2794 4011 4725
Size: 1420 1374 1217 714
Fragments arranged by size:
1420 1374 1217 714
XhoI C'TCGA_G
Cuts at: 0 3247 4725
Size: 3247 1478
XmaI C'CCGG_G
Cuts at: 0 941 1249 1389 1696 2334 3269 3765 3788
Size: 941 308 140 307 638 935 496 23
Cuts at: 3788 4563 4725
Size: 775 162
Fragments arranged by size:
941 935 775 638 496 308 307 162
140 23
ZraI GAC'GTC
Cuts at: 0 3183 4725
Size: 3183 1542
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AflIII AhdI
AleI AlfI AlwNI ApaI ApaLI ApoI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI
BfrBI BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrGI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI CspCI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PasI PflMI PfoI
PmlI PpiI PpuMI PshAI PspOMI PspXI PstI PvuII
SacI SacII SanDI SapI SbfI SexAI SfcI SfiI
SfoI SmaI SmlI SphI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI ZraI
Enzymes that do not cut:
AclI AflII AgeI AloI AscI AseI AsiSI BaeI
BclI BglII BmtI BplI BpuEI BsaBI BsaWI BsiWI
BspEI BspHI BssHII BstBI BstZ17I ClaI DraI DrdI
EcoRI EcoRV FalI FspI FspAI HpaI MfeI MluI
NdeI NheI NotI NruI PacI PciI PmeI PsiI
PsrI PvuI RsrII SalI ScaI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI XmnI