Restriction map for
pFN21AE6734
(Linear) (Six-base) MAPSORT of: KIEE6734.seq Check: 6681 from: 1 to: 2733
KIEE6734.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 519 2733
Size: 519 2214
AccI GT'mk_AC
Cuts at: 0 77 1982 2733
Size: 77 1905 751
Fragments arranged by size:
1905 751 77
Acc65I G'GTAC_C
Cuts at: 0 785 2733
Size: 785 1948
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 680 1198 1481 2070 2569 2733
Size: 228 452 518 283 589 499 164
Fragments arranged by size:
589 518 499 452 283 228 164
AfeI AGC'GCT
Cuts at: 0 1361 2733
Size: 1361 1372
AflIII A'CryG_T
Cuts at: 0 1289 1502 2733
Size: 1289 213 1231
Fragments arranged by size:
1289 1231 213
AhdI GACnn_n'nnGTC
Cuts at: 0 2266 2733
Size: 2266 467
AleI CACnn'nnGTG
Cuts at: 0 884 2733
Size: 884 1849
AlwNI CAG_nnn'CTG
Cuts at: 0 865 2212 2527 2654 2673 2733
Size: 865 1347 315 127 19 60
Fragments arranged by size:
1347 865 315 127 60 19
ApaI G_GGCC'C
Cuts at: 0 810 1251 1396 1654 2056 2733
Size: 810 441 145 258 402 677
Fragments arranged by size:
810 677 441 402 258 145
ApoI r'AATT_y
Cuts at: 0 172 1132 2733
Size: 172 960 1601
Fragments arranged by size:
1601 960 172
AvaI C'yCGr_G
Cuts at: 0 701 882 1064 1437 1715 2733
Size: 701 181 182 373 278 1018
Fragments arranged by size:
1018 701 373 278 182 181
BanI G'GyrC_C
Cuts at: 0 57 785 886 987 2733
Size: 57 728 101 101 1746
Fragments arranged by size:
1746 728 101 101 57
BanII G_rGCy'C
Cuts at: 0 810 845 1251 1396 1654 1739 2056 2184
Size: 810 35 406 145 258 85 317 128
Cuts at: 2184 2685 2733
Size: 501 48
Fragments arranged by size:
810 501 406 317 258 145 128 85
48 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 1285 1979 2733
Size: 1285 694 754
Fragments arranged by size:
1285 754 694
BbvCI CC'TCA_GC
Cuts at: 0 517 2733
Size: 517 2216
BglI GCCn_nnn'nGGC
Cuts at: 0 9 620 2733
Size: 9 611 2113
Fragments arranged by size:
2113 611 9
BglII A'GATC_T
Cuts at: 0 1529 2733
Size: 1529 1204
BlpI GC'TnA_GC
Cuts at: 0 949 2733
Size: 949 1784
Bme1580I G_kGCm'C
Cuts at: 0 62 810 891 992 1251 1396 1654 2056
Size: 62 748 81 101 259 145 258 402
Cuts at: 2056 2733
Size: 677
Fragments arranged by size:
748 677 402 259 258 145 101 81
62
BmgBI CAC'GTC
Cuts at: 0 42 267 2079 2733
Size: 42 225 1812 654
Fragments arranged by size:
1812 654 225 42
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1055 1548 2733
Size: 1055 493 1185
Fragments arranged by size:
1185 1055 493
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 481 745 1384 1630 1694 1807 2733
Size: 136 345 264 639 246 64 113 926
Fragments arranged by size:
926 639 345 264 246 136 113 64
Bpu10I CC'TnA_GC
Cuts at: 0 517 2008 2674 2733
Size: 517 1491 666 59
Fragments arranged by size:
1491 666 517 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1224 2733
Size: 1224 1509
BsaI GGTCTCn'nnnn_
Cuts at: 0 1026 1290 2158 2253 2733
Size: 1026 264 868 95 480
Fragments arranged by size:
1026 868 480 264 95
BsaAI yAC'GTr
Cuts at: 0 95 2733
Size: 95 2638
BsaHI Gr'CG_yC
Cuts at: 0 1001 2733
Size: 1001 1732
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1099 1129 2733
Size: 1099 30 1604
Fragments arranged by size:
1604 1099 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 415 601 682 1031 1540 1564 1885 2383
Size: 415 186 81 349 509 24 321 498
Cuts at: 2383 2733
Size: 350
Fragments arranged by size:
509 498 415 350 349 321 186 81
24
BseYI C'CCAG_C
Cuts at: 0 596 803 1334 1344 1558 2252 2733
Size: 596 207 531 10 214 694 481
Fragments arranged by size:
694 596 531 481 214 207 10
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 655 2733
Size: 64 591 2078
Fragments arranged by size:
2078 591 64
BsiHKAI G_wGCw'C
Cuts at: 0 407 2391 2733
Size: 407 1984 342
Fragments arranged by size:
1984 407 342
Bsp1286I G_dGCh'C
Cuts at: 0 62 407 810 845 891 992 1251 1396
Size: 62 345 403 35 46 101 259 145
Cuts at: 1396 1654 1739 2056 2184 2391 2685 2733
Size: 258 85 317 128 207 294 48
Fragments arranged by size:
403 345 317 294 259 258 207 145
128 101 85 62 48 46 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 519 1085 1871 2733
Size: 519 566 786 862
Fragments arranged by size:
862 786 566 519
BsrBI CCG'CTC
Cuts at: 0 1614 1620 1626 2322 2678 2733
Size: 1614 6 6 696 356 55
Fragments arranged by size:
1614 696 356 55 6 6
BsrFI r'CCGG_y
Cuts at: 0 125 2485 2733
Size: 125 2360 248
Fragments arranged by size:
2360 248 125
BssSI C'ACGA_G
Cuts at: 0 1324 2733
Size: 1324 1409
BstEII G'GTnAC_C
Cuts at: 0 430 1329 2733
Size: 430 899 1404
Fragments arranged by size:
1404 899 430
BstXI CCAn_nnnn'nTGG
Cuts at: 0 459 1308 2433 2733
Size: 459 849 1125 300
Fragments arranged by size:
1125 849 459 300
BstYI r'GATC_y
Cuts at: 0 1529 2208 2733
Size: 1529 679 525
Fragments arranged by size:
1529 679 525
Bsu36I CC'TnA_GG
Cuts at: 0 377 1483 1919 1964 1996 2733
Size: 377 1106 436 45 32 737
Fragments arranged by size:
1106 737 436 377 45 32
BtgI C'CryG_G
Cuts at: 0 7 179 452 1388 1447 1570 2476 2570
Size: 7 172 273 936 59 123 906 94
Cuts at: 2570 2733
Size: 163
Fragments arranged by size:
936 906 273 172 163 123 94 59
7
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1110 2733
Size: 1110 1623
BtsI GCAGTG_nn'
Cuts at: 0 578 2655 2733
Size: 578 2077 78
Fragments arranged by size:
2077 578 78
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1245 1280 2733
Size: 1245 35 1453
Fragments arranged by size:
1453 1245 35
DraIII CAC_nnn'GTG
Cuts at: 0 1329 2347 2733
Size: 1329 1018 386
Fragments arranged by size:
1329 1018 386
DrdI GACnn_nn'nnGTC
Cuts at: 0 271 454 2733
Size: 271 183 2279
Fragments arranged by size:
2279 271 183
EaeI y'GGCC_r
Cuts at: 0 1253 1794 1808 2118 2567 2573 2733
Size: 1253 541 14 310 449 6 160
Fragments arranged by size:
1253 541 449 310 160 14 6
EarI CTCTTCn'nnn_
Cuts at: 0 247 659 1106 2310 2733
Size: 247 412 447 1204 423
Fragments arranged by size:
1204 447 423 412 247
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 136 228 481 680 745 1198 1384 1481
Size: 136 92 253 199 65 453 186 97
Cuts at: 1481 1630 1694 1807 2070 2569 2733
Size: 149 64 113 263 499 164
Fragments arranged by size:
499 453 263 253 199 186 164 149
136 113 97 92 65 64
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1486 1867 2733
Size: 1486 381 866
Fragments arranged by size:
1486 866 381
EcoO109I rG'GnC_Cy
Cuts at: 0 198 309 381 600 807 862 1248 1393
Size: 198 111 72 219 207 55 386 145
Cuts at: 1393 1650 1651 2052 2053 2113 2670 2733
Size: 257 1 401 1 60 557 63
Fragments arranged by size:
557 401 386 257 219 207 198 145
111 72 63 60 55 1 1
EcoRI G'AATT_C
Cuts at: 0 1132 2733
Size: 1132 1601
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 258 2733
Size: 258 2475
HaeII r_GCGC'y
Cuts at: 0 132 1363 2733
Size: 132 1231 1370
Fragments arranged by size:
1370 1231 132
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 424 456 1047 1079 2193 2200 2225 2232
Size: 424 32 591 32 1114 7 25 7
Cuts at: 2232 2252 2284 2733
Size: 20 32 449
Fragments arranged by size:
1114 591 449 424 32 32 32 25
20 7 7
HincII GTy'rAC
Cuts at: 0 1983 2733
Size: 1983 750
HindIII A'AGCT_T
Cuts at: 0 2094 2733
Size: 2094 639
KpnI G_GTAC'C
Cuts at: 0 789 2733
Size: 789 1944
MfeI C'AATT_G
Cuts at: 0 290 2733
Size: 290 2443
MluI A'CGCG_T
Cuts at: 0 1289 2733
Size: 1289 1444
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 146 1574 2021 2450 2733
Size: 146 1428 447 429 283
Fragments arranged by size:
1428 447 429 283 146
MscI TGG'CCA
Cuts at: 0 1796 1810 2733
Size: 1796 14 923
Fragments arranged by size:
1796 923 14
MslI CAynn'nnrTG
Cuts at: 0 884 2733
Size: 884 1849
MspA1I CmG'CkG
Cuts at: 0 9 104 210 321 1572 1767 2518 2733
Size: 9 95 106 111 1251 195 751 215
Fragments arranged by size:
1251 751 215 195 111 106 95 9
NcoI C'CATG_G
Cuts at: 0 179 452 1447 2476 2733
Size: 179 273 995 1029 257
Fragments arranged by size:
1029 995 273 257 179
NspI r_CATG'y
Cuts at: 0 1506 2733
Size: 1506 1227
PciI A'CATG_T
Cuts at: 0 1502 2733
Size: 1502 1231
PflMI CCAn_nnn'nTGG
Cuts at: 0 865 2079 2733
Size: 865 1214 654
Fragments arranged by size:
1214 865 654
PfoI T'CCnGG_A
Cuts at: 0 260 458 2609 2633 2733
Size: 260 198 2151 24 100
Fragments arranged by size:
2151 260 198 100 24
PmlI CAC'GTG
Cuts at: 0 95 2733
Size: 95 2638
PpuMI rG'GwC_Cy
Cuts at: 0 198 381 600 2670 2733
Size: 198 183 219 2070 63
Fragments arranged by size:
2070 219 198 183 63
PspOMI G'GGCC_C
Cuts at: 0 806 1247 1392 1650 2052 2733
Size: 806 441 145 258 402 681
Fragments arranged by size:
806 681 441 402 258 145
PspXI vC'TCGA_Gb
Cuts at: 0 1064 2733
Size: 1064 1669
PstI C_TGCA'G
Cuts at: 0 359 656 2179 2523 2733
Size: 359 297 1523 344 210
Fragments arranged by size:
1523 359 344 297 210
PvuII CAG'CTG
Cuts at: 0 321 1767 2518 2733
Size: 321 1446 751 215
Fragments arranged by size:
1446 751 321 215
SacII CC_GC'GG
Cuts at: 0 10 1573 2733
Size: 10 1563 1160
Fragments arranged by size:
1563 1160 10
SalI G'TCGA_C
Cuts at: 0 1981 2733
Size: 1981 752
SanDI GG'GwC_CC
Cuts at: 0 600 2733
Size: 600 2133
SfcI C'TryA_G
Cuts at: 0 132 355 652 2175 2519 2733
Size: 132 223 297 1523 344 214
Fragments arranged by size:
1523 344 297 223 214 132
SmaI CCC'GGG
Cuts at: 0 884 1717 2733
Size: 884 833 1016
Fragments arranged by size:
1016 884 833
SmlI C'TyrA_G
Cuts at: 0 1064 1203 2733
Size: 1064 139 1530
Fragments arranged by size:
1530 1064 139
StuI AGG'CCT
Cuts at: 0 1070 1175 2013 2733
Size: 1070 105 838 720
Fragments arranged by size:
1070 838 720 105
StyI C'CwwG_G
Cuts at: 0 179 452 1447 1811 2476 2733
Size: 179 273 995 364 665 257
Fragments arranged by size:
995 665 364 273 257 179
TatI w'GTAC_w
Cuts at: 0 674 1271 2733
Size: 674 597 1462
Fragments arranged by size:
1462 674 597
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 757 789 2733
Size: 757 32 1944
Fragments arranged by size:
1944 757 32
Tth111I GACn'n_nGTC
Cuts at: 0 1978 2733
Size: 1978 755
XhoI C'TCGA_G
Cuts at: 0 1064 2733
Size: 1064 1669
XmaI C'CCGG_G
Cuts at: 0 882 1715 2733
Size: 882 833 1018
Fragments arranged by size:
1018 882 833
XmnI GAAnn'nnTTC
Cuts at: 0 1900 2733
Size: 1900 833
Enzymes that do cut:
AarI AccI Acc65I AcuI AfeI AflIII AhdI AleI
AlwNI ApaI ApoI AvaI BanI BanII BbsI BbvCI
BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssSI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EarI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII
Hin4I HincII HindIII KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NspI PciI PflMI PfoI PmlI
PpuMI PspOMI PspXI PstI PvuII SacII SalI SanDI
SfcI SmaI SmlI StuI StyI TatI TstI Tth111I
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AflII AgeI AlfI AloI ApaLI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BcgI BciVI
BclI BfrBI BmrI BmtI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII BstAPI
BstBI BstZ17I ClaI DraI EagI EciI EcoICRI EcoRV
FseI FspI FspAI HpaI KasI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PmeI
PpiI PshAI PsiI PsrI PvuI RsrII SacI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI
SpeI SphI SrfI SspI SwaI TaqII XbaI XcmI
ZraI