Restriction map for
pFN21AE6730
(Linear) (Six-base) MAPSORT of: KIEE6730.seq Check: 7814 from: 1 to: 2427
KIEE6730.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 790 833 1002 1903 2427
Size: 790 43 169 901 524
Fragments arranged by size:
901 790 524 169 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 2290 2358 2427
Size: 109 2181 68 69
Fragments arranged by size:
2181 109 69 68
AflIII A'CryG_T
Cuts at: 0 1583 2427
Size: 1583 844
AhdI GACnn_n'nnGTC
Cuts at: 0 960 2427
Size: 960 1467
AleI CACnn'nnGTG
Cuts at: 0 496 1923 2095 2427
Size: 496 1427 172 332
Fragments arranged by size:
1427 496 332 172
AlwNI CAG_nnn'CTG
Cuts at: 0 42 81 115 214 754 2007 2427
Size: 42 39 34 99 540 1253 420
Fragments arranged by size:
1253 540 420 99 42 39 34
ApaI G_GGCC'C
Cuts at: 0 18 174 190 773 1163 1487 1523 2427
Size: 18 156 16 583 390 324 36 904
Fragments arranged by size:
904 583 390 324 156 36 18 16
ApaLI G'TGCA_C
Cuts at: 0 1327 2427
Size: 1327 1100
AvaI C'yCGr_G
Cuts at: 0 934 1302 1427 1516 1572 1591 1873 1977
Size: 934 368 125 89 56 19 282 104
Cuts at: 1977 1987 2331 2427
Size: 10 344 96
Fragments arranged by size:
934 368 344 282 125 104 96 89
56 19 10
BamHI G'GATC_C
Cuts at: 0 71 1185 2427
Size: 71 1114 1242
Fragments arranged by size:
1242 1114 71
BanI G'GyrC_C
Cuts at: 0 655 709 812 1509 1799 2427
Size: 655 54 103 697 290 628
Fragments arranged by size:
697 655 628 290 103 54
BanII G_rGCy'C
Cuts at: 0 18 174 190 773 1154 1163 1487 1523
Size: 18 156 16 583 381 9 324 36
Cuts at: 1523 2162 2338 2427
Size: 639 176 89
Fragments arranged by size:
639 583 381 324 176 156 89 36
18 16 9
BbeI G_GCGC'C
Cuts at: 0 816 2427
Size: 816 1611
BbvCI CC'TCA_GC
Cuts at: 0 178 806 1454 2427
Size: 178 628 648 973
Fragments arranged by size:
973 648 628 178
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1106 1140 2427
Size: 1106 34 1287
Fragments arranged by size:
1287 1106 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2394 2427
Size: 2394 33
BglI GCCn_nnn'nGGC
Cuts at: 0 145 653 1158 2427
Size: 145 508 505 1269
Fragments arranged by size:
1269 508 505 145
BlpI GC'TnA_GC
Cuts at: 0 58 2427
Size: 58 2369
Bme1580I G_kGCm'C
Cuts at: 0 18 174 190 269 589 773 1113 1163
Size: 18 156 16 79 320 184 340 50
Cuts at: 1163 1331 1487 1523 1591 1654 1802 2102 2427
Size: 168 156 36 68 63 148 300 325
Fragments arranged by size:
340 325 320 300 184 168 156 156
148 79 68 63 50 36 18 16
BmgBI CAC'GTC
Cuts at: 0 558 1332 1842 2427
Size: 558 774 510 585
Fragments arranged by size:
774 585 558 510
BmrI ACTGGGnnnn_n'
Cuts at: 0 954 2109 2427
Size: 954 1155 318
Fragments arranged by size:
1155 954 318
BmtI G_CTAG'C
Cuts at: 0 235 2427
Size: 235 2192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1168 1638 2427
Size: 1168 470 789
Fragments arranged by size:
1168 789 470
Bpu10I CC'TnA_GC
Cuts at: 0 178 520 806 879 1454 2427
Size: 178 342 286 73 575 973
Fragments arranged by size:
973 575 342 286 178 73
BsaI GGTCTCn'nnnn_
Cuts at: 0 118 628 846 949 2427
Size: 118 510 218 103 1478
Fragments arranged by size:
1478 510 218 118 103
BsaAI yAC'GTr
Cuts at: 0 972 1586 2427
Size: 972 614 841
Fragments arranged by size:
972 841 614
BsaHI Gr'CG_yC
Cuts at: 0 813 956 1817 2427
Size: 813 143 861 610
Fragments arranged by size:
861 813 610 143
BsaWI w'CCGG_w
Cuts at: 0 219 1605 2427
Size: 219 1386 822
Fragments arranged by size:
1386 822 219
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 967 997 2427
Size: 967 30 1430
Fragments arranged by size:
1430 967 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 112 195 458 463 466 646 1051
Size: 109 3 83 263 5 3 180 405
Cuts at: 1051 1467 2170 2427
Size: 416 703 257
Fragments arranged by size:
703 416 405 263 257 180 109 83
5 3 3
BseYI C'CCAG_C
Cuts at: 0 17 35 403 507 685 1420 1439 2395
Size: 17 18 368 104 178 735 19 956
Cuts at: 2395 2427
Size: 32
Fragments arranged by size:
956 735 368 178 104 32 19 18
17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 805 811 2427
Size: 559 246 6 1616
Fragments arranged by size:
1616 559 246 6
BsiEI CG_ry'CG
Cuts at: 0 248 544 2427
Size: 248 296 1883
Fragments arranged by size:
1883 296 248
BsiHKAI G_wGCw'C
Cuts at: 0 1331 1661 2162 2427
Size: 1331 330 501 265
Fragments arranged by size:
1331 501 330 265
BsmBI CGTCTCn'nnnn_
Cuts at: 0 448 2427
Size: 448 1979
Bsp1286I G_dGCh'C
Cuts at: 0 18 174 190 269 589 773 1113 1154
Size: 18 156 16 79 320 184 340 41
Cuts at: 1154 1163 1331 1487 1523 1591 1654 1661 1802
Size: 9 168 156 36 68 63 7 141
Cuts at: 1802 2102 2162 2338 2427
Size: 300 60 176 89
Fragments arranged by size:
340 320 300 184 176 168 156 156
141 89 79 68 63 60 41 36
18 16 9 7
BspEI T'CCGG_A
Cuts at: 0 219 1605 2427
Size: 219 1386 822
Fragments arranged by size:
1386 822 219
BspHI T'CATG_A
Cuts at: 0 1763 2427
Size: 1763 664
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 212 581 790 833 1002 1903 2427
Size: 212 369 209 43 169 901 524
Fragments arranged by size:
901 524 369 212 209 169 43
BsrBI CCG'CTC
Cuts at: 0 939 2427
Size: 939 1488
BsrFI r'CCGG_y
Cuts at: 0 247 543 815 863 1218 1819 2427
Size: 247 296 272 48 355 601 608
Fragments arranged by size:
608 601 355 296 272 247 48
BsrGI T'GTAC_A
Cuts at: 0 2009 2427
Size: 2009 418
BssSI C'ACGA_G
Cuts at: 0 1125 2427
Size: 1125 1302
BstEII G'GTnAC_C
Cuts at: 0 498 2082 2427
Size: 498 1584 345
Fragments arranged by size:
1584 498 345
BstXI CCAn_nnnn'nTGG
Cuts at: 0 693 2361 2427
Size: 693 1668 66
Fragments arranged by size:
1668 693 66
BstYI r'GATC_y
Cuts at: 0 71 211 1185 2199 2427
Size: 71 140 974 1014 228
Fragments arranged by size:
1014 974 228 140 71
Bsu36I CC'TnA_GG
Cuts at: 0 437 2427
Size: 437 1990
BtgI C'CryG_G
Cuts at: 0 131 277 549 1085 1632 2360 2427
Size: 131 146 272 536 547 728 67
Fragments arranged by size:
728 547 536 272 146 131 67
BtsI GCAGTG_nn'
Cuts at: 0 269 350 587 1495 2283 2427
Size: 269 81 237 908 788 144
Fragments arranged by size:
908 788 269 237 144 81
EaeI y'GGCC_r
Cuts at: 0 245 249 541 661 2103 2237 2427
Size: 245 4 292 120 1442 134 190
Fragments arranged by size:
1442 292 245 190 134 120 4
EagI C'GGCC_G
Cuts at: 0 245 541 2427
Size: 245 296 1886
Fragments arranged by size:
1886 296 245
EarI CTCTTCn'nnn_
Cuts at: 0 1334 2427
Size: 1334 1093
EcoICRI GAG'CTC
Cuts at: 0 2160 2427
Size: 2160 267
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 1168 1638 2290 2358 2427
Size: 109 1059 470 652 68 69
Fragments arranged by size:
1059 652 470 109 69 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 91 440 1540 2427
Size: 91 349 1100 887
Fragments arranged by size:
1100 887 349 91
EcoO109I rG'GnC_Cy
Cuts at: 0 14 147 170 171 186 301 346 421
Size: 14 133 23 1 15 115 45 75
Cuts at: 421 769 1160 1450 1520 1569 1638 1722 1938
Size: 348 391 290 70 49 69 84 216
Cuts at: 1938 2427
Size: 489
Fragments arranged by size:
489 391 348 290 216 133 115 84
75 70 69 49 45 23 15 14
1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1368 2427
Size: 1368 1059
FseI GG_CCGG'CC
Cuts at: 0 251 547 2427
Size: 251 296 1880
Fragments arranged by size:
1880 296 251
HaeII r_GCGC'y
Cuts at: 0 816 1427 1739 2427
Size: 816 611 312 688
Fragments arranged by size:
816 688 611 312
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 63 95 288 320 946 967 978 999
Size: 63 32 193 32 626 21 11 21
Cuts at: 999 2184 2216 2427
Size: 1185 32 211
Fragments arranged by size:
1185 626 211 193 63 32 32 32
21 21 11
HindIII A'AGCT_T
Cuts at: 0 2302 2427
Size: 2302 125
KasI G'GCGC_C
Cuts at: 0 812 2427
Size: 812 1615
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1232 1724 2348 2427
Size: 1232 492 624 79
Fragments arranged by size:
1232 624 492 79
MscI TGG'CCA
Cuts at: 0 2239 2427
Size: 2239 188
MslI CAynn'nnrTG
Cuts at: 0 496 1073 1923 2095 2359 2427
Size: 496 577 850 172 264 68
Fragments arranged by size:
850 577 496 264 172 68
MspA1I CmG'CkG
Cuts at: 0 39 621 689 805 1261 1443 1853 2214
Size: 39 582 68 116 456 182 410 361
Cuts at: 2214 2385 2427
Size: 171 42
Fragments arranged by size:
582 456 410 361 182 171 116 68
42 39
NaeI GCC'GGC
Cuts at: 0 249 545 817 1220 1821 2427
Size: 249 296 272 403 601 606
Fragments arranged by size:
606 601 403 296 272 249
NarI GG'CG_CC
Cuts at: 0 813 2427
Size: 813 1614
NcoI C'CATG_G
Cuts at: 0 549 2427
Size: 549 1878
NgoMIV G'CCGG_C
Cuts at: 0 247 543 815 1218 1819 2427
Size: 247 296 272 403 601 608
Fragments arranged by size:
608 601 403 296 272 247
NheI G'CTAG_C
Cuts at: 0 231 2427
Size: 231 2196
NspI r_CATG'y
Cuts at: 0 1395 2427
Size: 1395 1032
PasI CC'CwG_GG
Cuts at: 0 11 418 580 729 1105 1156 2427
Size: 11 407 162 149 376 51 1271
Fragments arranged by size:
1271 407 376 162 149 51 11
PflMI CCAn_nnn'nTGG
Cuts at: 0 115 754 2427
Size: 115 639 1673
Fragments arranged by size:
1673 639 115
PfoI T'CCnGG_A
Cuts at: 0 667 1334 2162 2427
Size: 667 667 828 265
Fragments arranged by size:
828 667 667 265
PmlI CAC'GTG
Cuts at: 0 1586 2427
Size: 1586 841
PpuMI rG'GwC_Cy
Cuts at: 0 1450 1569 1638 1722 1938 2427
Size: 1450 119 69 84 216 489
Fragments arranged by size:
1450 489 216 119 84 69
PspOMI G'GGCC_C
Cuts at: 0 14 170 186 769 1159 1483 1519 2427
Size: 14 156 16 583 390 324 36 908
Fragments arranged by size:
908 583 390 324 156 36 16 14
PspXI vC'TCGA_Gb
Cuts at: 0 1873 2427
Size: 1873 554
PstI C_TGCA'G
Cuts at: 0 626 1502 2427
Size: 626 876 925
Fragments arranged by size:
925 876 626
PvuII CAG'CTG
Cuts at: 0 39 621 689 805 1261 1443 2214 2427
Size: 39 582 68 116 456 182 771 213
Fragments arranged by size:
771 582 456 213 182 116 68 39
SacI G_AGCT'C
Cuts at: 0 2162 2427
Size: 2162 265
SanDI GG'GwC_CC
Cuts at: 0 1938 2427
Size: 1938 489
SfcI C'TryA_G
Cuts at: 0 622 1296 1498 1899 2346 2427
Size: 622 674 202 401 447 81
Fragments arranged by size:
674 622 447 401 202 81
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 145 2427
Size: 145 2282
SfoI GGC'GCC
Cuts at: 0 814 2427
Size: 814 1613
SmaI CCC'GGG
Cuts at: 0 1304 1518 1979 2427
Size: 1304 214 461 448
Fragments arranged by size:
1304 461 448 214
SmlI C'TyrA_G
Cuts at: 0 1873 2427
Size: 1873 554
SrfI GCCC'GGGC
Cuts at: 0 1304 1518 2427
Size: 1304 214 909
Fragments arranged by size:
1304 909 214
StuI AGG'CCT
Cuts at: 0 1535 2427
Size: 1535 892
StyI C'CwwG_G
Cuts at: 0 429 549 2427
Size: 429 120 1878
Fragments arranged by size:
1878 429 120
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 417 1935 2427
Size: 417 1518 492
Fragments arranged by size:
1518 492 417
TatI w'GTAC_w
Cuts at: 0 2009 2427
Size: 2009 418
Tth111I GACn'n_nGTC
Cuts at: 0 928 2427
Size: 928 1499
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1179 1607 1675 2427
Size: 1179 428 68 752
Fragments arranged by size:
1179 752 428 68
XhoI C'TCGA_G
Cuts at: 0 1873 2427
Size: 1873 554
XmaI C'CCGG_G
Cuts at: 0 1302 1516 1977 2427
Size: 1302 214 461 450
Fragments arranged by size:
1302 461 450 214
XmnI GAAnn'nnTTC
Cuts at: 0 161 2427
Size: 161 2266
Enzymes that do cut:
AarI AcuI AflIII AhdI AleI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbvCI BcgI BciVI
BglI BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I
BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssSI BstEII BstXI BstYI Bsu36I BtgI
BtsI EaeI EagI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI FseI HaeII Hin4I HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NheI NspI
PasI PflMI PfoI PmlI PpuMI PspOMI PspXI PstI
PvuII SacI SanDI SfcI SfiI SfoI SmaI SmlI
SrfI StuI StyI TaqII TatI Tth111I XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BbsI
BclI BfrBI BglII BplI BpuEI BsaBI BsiWI BsmI
BsrDI BssHII BstAPI BstBI BstZ17I BtgZI ClaI CspCI
DraI DraIII DrdI EciI EcoRI EcoRV FspI FspAI
HincII HpaI KpnI MfeI MluI NdeI NotI NruI
NsiI PacI PciI PmeI PpiI PshAI PsiI PsrI
PvuI RsrII SacII SalI SapI SbfI ScaI SexAI
SgfI SgrAI SnaBI SpeI SphI SspI SwaI TstI
XbaI ZraI