Restriction map for
pFN21AE6729
(Linear) (Six-base) MAPSORT of: KIEE6729.seq Check: 9609 from: 1 to: 3255
KIEE6729.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 433 3255
Size: 433 2822
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1231 1383 2113 3255
Size: 1231 152 730 1142
Fragments arranged by size:
1231 1142 730 152
AfeI AGC'GCT
Cuts at: 0 2013 2518 3255
Size: 2013 505 737
Fragments arranged by size:
2013 737 505
AflIII A'CryG_T
Cuts at: 0 725 1543 2540 3255
Size: 725 818 997 715
Fragments arranged by size:
997 818 725 715
AleI CACnn'nnGTG
Cuts at: 0 16 2561 3064 3255
Size: 16 2545 503 191
Fragments arranged by size:
2545 503 191 16
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1613 2590 3255
Size: 1613 977 665
Fragments arranged by size:
1613 977 665
AlwNI CAG_nnn'CTG
Cuts at: 0 1117 2082 2805 3255
Size: 1117 965 723 450
Fragments arranged by size:
1117 965 723 450
ApaLI G'TGCA_C
Cuts at: 0 2554 3019 3255
Size: 2554 465 236
Fragments arranged by size:
2554 465 236
ApoI r'AATT_y
Cuts at: 0 586 823 3255
Size: 586 237 2432
Fragments arranged by size:
2432 586 237
AvaI C'yCGr_G
Cuts at: 0 58 947 1105 1203 3178 3255
Size: 58 889 158 98 1975 77
Fragments arranged by size:
1975 889 158 98 77 58
BamHI G'GATC_C
Cuts at: 0 1991 3255
Size: 1991 1264
BanI G'GyrC_C
Cuts at: 0 18 283 319 342 355 1420 1575 3255
Size: 18 265 36 23 13 1065 155 1680
Fragments arranged by size:
1680 1065 265 155 36 23 18 13
BanII G_rGCy'C
Cuts at: 0 68 553 3255
Size: 68 485 2702
Fragments arranged by size:
2702 485 68
BbeI G_GCGC'C
Cuts at: 0 287 3255
Size: 287 2968
BbsI GAAGACnn'nnnn_
Cuts at: 0 2431 3255
Size: 2431 824
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1692 1726 3255
Size: 1692 34 1529
Fragments arranged by size:
1692 1529 34
BclI T'GATC_A
Cuts at: 0 851 3255
Size: 851 2404
BfrBI ATG'CAT
Cuts at: 0 923 2739 3255
Size: 923 1816 516
Fragments arranged by size:
1816 923 516
BglI GCCn_nnn'nGGC
Cuts at: 0 1918 2351 2467 3255
Size: 1918 433 116 788
Fragments arranged by size:
1918 788 433 116
BglII A'GATC_T
Cuts at: 0 665 3255
Size: 665 2590
Bme1580I G_kGCm'C
Cuts at: 0 322 358 439 1492 1578 1671 2483 2558
Size: 322 36 81 1053 86 93 812 75
Cuts at: 2558 3023 3255
Size: 465 232
Fragments arranged by size:
1053 812 465 322 232 93 86 81
75 36
BmgBI CAC'GTC
Cuts at: 0 728 2541 2948 3255
Size: 728 1813 407 307
Fragments arranged by size:
1813 728 407 307
BmrI ACTGGGnnnn_n'
Cuts at: 0 586 1854 3255
Size: 586 1268 1401
Fragments arranged by size:
1401 1268 586
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1241 3255
Size: 1241 2014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 1174 1543 2059 2545 2631 2905 3255
Size: 1111 63 369 516 486 86 274 350
Fragments arranged by size:
1111 516 486 369 350 274 86 63
Bpu10I CC'TnA_GC
Cuts at: 0 2008 2225 3255
Size: 2008 217 1030
Fragments arranged by size:
2008 1030 217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2137 2731 3220 3255
Size: 2137 594 489 35
Fragments arranged by size:
2137 594 489 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 2968 3255
Size: 2968 287
BsaAI yAC'GTr
Cuts at: 0 1233 2115 3255
Size: 1233 882 1140
Fragments arranged by size:
1233 1140 882
BsaBI GATnn'nnATC
Cuts at: 0 2629 3255
Size: 2629 626
BsaHI Gr'CG_yC
Cuts at: 0 284 1337 3104 3255
Size: 284 1053 1767 151
Fragments arranged by size:
1767 1053 284 151
BsaWI w'CCGG_w
Cuts at: 0 915 3255
Size: 915 2340
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 443 473 584 614 2779 2809 2878 2908
Size: 443 30 111 30 2165 30 69 30
Cuts at: 2908 3255
Size: 347
Fragments arranged by size:
2165 443 347 111 69 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 313 451 1663 1887 2042 2056 2122 3255
Size: 313 138 1212 224 155 14 66 1133
Fragments arranged by size:
1212 1133 313 224 155 138 66 14
BseYI C'CCAG_C
Cuts at: 0 346 544 877 927 1007 1698 2147 2376
Size: 346 198 333 50 80 691 449 229
Cuts at: 2376 2503 2849 2986 3255
Size: 127 346 137 269
Fragments arranged by size:
691 449 346 346 333 269 229 198
137 127 80 50
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1813 2433 3255
Size: 1813 620 822
Fragments arranged by size:
1813 822 620
BsiEI CG_ry'CG
Cuts at: 0 1049 2074 2797 3255
Size: 1049 1025 723 458
Fragments arranged by size:
1049 1025 723 458
BsiHKAI G_wGCw'C
Cuts at: 0 720 1127 2558 3023 3255
Size: 720 407 1431 465 232
Fragments arranged by size:
1431 720 465 407 232
BsmI GAATG_Cn'
Cuts at: 0 738 3255
Size: 738 2517
BsmBI CGTCTCn'nnnn_
Cuts at: 0 245 3255
Size: 245 3010
Bsp1286I G_dGCh'C
Cuts at: 0 68 322 358 439 553 720 1127 1492
Size: 68 254 36 81 114 167 407 365
Cuts at: 1492 1578 1671 2483 2558 3023 3255
Size: 86 93 812 75 465 232
Fragments arranged by size:
812 465 407 365 254 232 167 114
93 86 81 75 68 36
BspHI T'CATG_A
Cuts at: 0 560 720 782 3255
Size: 560 160 62 2473
Fragments arranged by size:
2473 560 160 62
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 383 433 3255
Size: 383 50 2822
Fragments arranged by size:
2822 383 50
BsrBI CCG'CTC
Cuts at: 0 1319 2772 2835 3204 3255
Size: 1319 1453 63 369 51
Fragments arranged by size:
1453 1319 369 63 51
BsrDI GCAATG_nn'
Cuts at: 0 680 1595 3255
Size: 680 915 1660
Fragments arranged by size:
1660 915 680
BsrFI r'CCGG_y
Cuts at: 0 1933 3255
Size: 1933 1322
BsrGI T'GTAC_A
Cuts at: 0 644 3255
Size: 644 2611
BssHII G'CGCG_C
Cuts at: 0 90 3255
Size: 90 3165
BssSI C'ACGA_G
Cuts at: 0 1390 2476 2512 3255
Size: 1390 1086 36 743
Fragments arranged by size:
1390 1086 743 36
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1185 2805 3255
Size: 1185 1620 450
Fragments arranged by size:
1620 1185 450
BstXI CCAn_nnnn'nTGG
Cuts at: 0 534 2341 3255
Size: 534 1807 914
Fragments arranged by size:
1807 914 534
BstYI r'GATC_y
Cuts at: 0 665 1091 1991 2936 3255
Size: 665 426 900 945 319
Fragments arranged by size:
945 900 665 426 319
Bsu36I CC'TnA_GG
Cuts at: 0 1804 2878 3255
Size: 1804 1074 377
Fragments arranged by size:
1804 1074 377
BtgI C'CryG_G
Cuts at: 0 14 1638 2125 2369 3255
Size: 14 1624 487 244 886
Fragments arranged by size:
1624 886 487 244 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 504 1230 1475 1674 1695 1914 2475 3255
Size: 504 726 245 199 21 219 561 780
Fragments arranged by size:
780 726 561 504 245 219 199 21
BtsI GCAGTG_nn'
Cuts at: 0 1410 2927 3059 3255
Size: 1410 1517 132 196
Fragments arranged by size:
1517 1410 196 132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1568 1603 3255
Size: 1568 35 1652
Fragments arranged by size:
1652 1568 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 2686 3255
Size: 2686 569
EaeI y'GGCC_r
Cuts at: 0 500 1001 1370 1635 1919 2071 2297 3228
Size: 500 501 369 265 284 152 226 931
Cuts at: 3228 3255
Size: 27
Fragments arranged by size:
931 501 500 369 284 265 226 152
27
EagI C'GGCC_G
Cuts at: 0 2071 3255
Size: 2071 1184
EarI CTCTTCn'nnn_
Cuts at: 0 206 248 415 841 3255
Size: 206 42 167 426 2414
Fragments arranged by size:
2414 426 206 167 42
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 214 3255
Size: 214 3041
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1111 1174 1231 1383 1543 2059 2113 2545
Size: 1111 63 57 152 160 516 54 432
Cuts at: 2545 2631 2905 3255
Size: 86 274 350
Fragments arranged by size:
1111 516 432 350 274 160 152 86
63 57 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 277 1156 1588 3255
Size: 277 879 432 1667
Fragments arranged by size:
1667 879 432 277
EcoO109I rG'GnC_Cy
Cuts at: 0 456 938 1326 1808 2047 2106 2166 2409
Size: 456 482 388 482 239 59 60 243
Cuts at: 2409 2679 2982 3195 3255
Size: 270 303 213 60
Fragments arranged by size:
482 482 456 388 303 270 243 239
213 60 60 59
FspI TGC'GCA
Cuts at: 0 2629 3255
Size: 2629 626
FspAI rTGC'GCAy
Cuts at: 0 2629 3255
Size: 2629 626
HaeII r_GCGC'y
Cuts at: 0 287 1148 1553 1852 2015 2520 3255
Size: 287 861 405 299 163 505 735
Fragments arranged by size:
861 735 505 405 299 287 163
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 2779 2811 2854 2886 3255
Size: 2779 32 43 32 369
Fragments arranged by size:
2779 369 43 32 32
KasI G'GCGC_C
Cuts at: 0 283 3255
Size: 283 2972
MscI TGG'CCA
Cuts at: 0 1003 1372 1637 1921 2299 3255
Size: 1003 369 265 284 378 956
Fragments arranged by size:
1003 956 378 369 284 265
MslI CAynn'nnrTG
Cuts at: 0 16 361 367 508 2539 2561 2572 2668
Size: 16 345 6 141 2031 22 11 96
Cuts at: 2668 2744 3064 3255
Size: 76 320 191
Fragments arranged by size:
2031 345 320 191 141 96 76 22
16 11 6
MspA1I CmG'CkG
Cuts at: 0 544 694 979 1114 2079 2127 2793 2853
Size: 544 150 285 135 965 48 666 60
Cuts at: 2853 3255
Size: 402
Fragments arranged by size:
965 666 544 402 285 150 135 60
48
NaeI GCC'GGC
Cuts at: 0 1935 3255
Size: 1935 1320
NarI GG'CG_CC
Cuts at: 0 284 3255
Size: 284 2971
NgoMIV G'CCGG_C
Cuts at: 0 1933 3255
Size: 1933 1322
NotI GC'GGCC_GC
Cuts at: 0 2071 3255
Size: 2071 1184
NsiI A_TGCA'T
Cuts at: 0 925 2741 3255
Size: 925 1816 514
Fragments arranged by size:
1816 925 514
NspI r_CATG'y
Cuts at: 0 927 2358 2571 3057 3129 3255
Size: 927 1431 213 486 72 126
Fragments arranged by size:
1431 927 486 213 126 72
PasI CC'CwG_GG
Cuts at: 0 279 1511 3255
Size: 279 1232 1744
Fragments arranged by size:
1744 1232 279
PflMI CCAn_nnn'nTGG
Cuts at: 0 589 2066 2292 3255
Size: 589 1477 226 963
Fragments arranged by size:
1477 963 589 226
PfoI T'CCnGG_A
Cuts at: 0 1127 1445 3008 3255
Size: 1127 318 1563 247
Fragments arranged by size:
1563 1127 318 247
PmlI CAC'GTG
Cuts at: 0 2115 3255
Size: 2115 1140
PpuMI rG'GwC_Cy
Cuts at: 0 938 1326 1808 2106 2409 2679 2982 3255
Size: 938 388 482 298 303 270 303 273
Fragments arranged by size:
938 482 388 303 303 298 273 270
PspXI vC'TCGA_Gb
Cuts at: 0 58 3178 3255
Size: 58 3120 77
Fragments arranged by size:
3120 77 58
PstI C_TGCA'G
Cuts at: 0 389 2922 3255
Size: 389 2533 333
Fragments arranged by size:
2533 389 333
PvuII CAG'CTG
Cuts at: 0 544 694 979 1114 2079 2853 3255
Size: 544 150 285 135 965 774 402
Fragments arranged by size:
965 774 544 402 285 150 135
SacII CC_GC'GG
Cuts at: 0 2128 3255
Size: 2128 1127
SanDI GG'GwC_CC
Cuts at: 0 2982 3255
Size: 2982 273
SapI GCTCTTCn'nnn_
Cuts at: 0 206 841 3255
Size: 206 635 2414
Fragments arranged by size:
2414 635 206
ScaI AGT'ACT
Cuts at: 0 1837 3255
Size: 1837 1418
SfcI C'TryA_G
Cuts at: 0 385 451 980 2918 3255
Size: 385 66 529 1938 337
Fragments arranged by size:
1938 529 385 337 66
SfoI GGC'GCC
Cuts at: 0 285 3255
Size: 285 2970
SmaI CCC'GGG
Cuts at: 0 949 1107 3255
Size: 949 158 2148
Fragments arranged by size:
2148 949 158
SmlI C'TyrA_G
Cuts at: 0 58 2152 2710 3178 3199 3255
Size: 58 2094 558 468 21 56
Fragments arranged by size:
2094 558 468 58 56 21
SphI G_CATG'C
Cuts at: 0 927 2358 2571 3057 3255
Size: 927 1431 213 486 198
Fragments arranged by size:
1431 927 486 213 198
StuI AGG'CCT
Cuts at: 0 2000 3255
Size: 2000 1255
StyI C'CwwG_G
Cuts at: 0 155 1601 1962 3255
Size: 155 1446 361 1293
Fragments arranged by size:
1446 1293 361 155
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 63 292 360 1021 1063 1102 3153 3205
Size: 63 229 68 661 42 39 2051 52
Cuts at: 3205 3255
Size: 50
Fragments arranged by size:
2051 661 229 68 63 52 50 42
39
TatI w'GTAC_w
Cuts at: 0 644 1394 1835 2894 2963 3255
Size: 644 750 441 1059 69 292
Fragments arranged by size:
1059 750 644 441 292 69
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 584 616 1123 1155 3255
Size: 584 32 507 32 2100
Fragments arranged by size:
2100 584 507 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 942 3255
Size: 942 2313
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 531 648 2155 3255
Size: 531 117 1507 1100
Fragments arranged by size:
1507 1100 531 117
XhoI C'TCGA_G
Cuts at: 0 58 3178 3255
Size: 58 3120 77
Fragments arranged by size:
3120 77 58
XmaI C'CCGG_G
Cuts at: 0 947 1105 3255
Size: 947 158 2150
Fragments arranged by size:
2150 947 158
XmnI GAAnn'nnTTC
Cuts at: 0 2278 2428 3255
Size: 2278 150 827
Fragments arranged by size:
2278 827 150
Enzymes that do cut:
AarI AcuI AfeI AflIII AleI AlfI AlwNI ApaLI
ApoI AvaI BamHI BanI BanII BbeI BbsI BcgI
BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
CspCI DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I FspI FspAI HaeII Hin4I KasI MscI MslI
MspA1I NaeI NarI NgoMIV NotI NsiI NspI PasI
PflMI PfoI PmlI PpuMI PspXI PstI PvuII SacII
SanDI SapI ScaI SfcI SfoI SmaI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XhoI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AloI
ApaI AscI AseI AsiSI AvrII BaeI BbvCI BciVI
BlpI BmtI BsiWI BspEI BstBI BstEII BstZ17I ClaI
DraI DraIII EcoICRI EcoRI EcoRV FalI FseI HincII
HindIII HpaI KpnI MfeI MluI MmeI NcoI NdeI
NheI NruI PacI PciI PmeI PpiI PshAI PsiI
PspOMI PsrI PvuI RsrII SacI SalI SbfI SexAI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
XbaI ZraI