Restriction map for
pFN21AE6726
(Linear) (Six-base) MAPSORT of: KIEE6726.seq Check: 2020 from: 1 to: 2094
KIEE6726.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AccI GT'mk_AC
Cuts at: 0 821 2094
Size: 821 1273
Acc65I G'GTAC_C
Cuts at: 0 1166 2094
Size: 1166 928
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 343 526 553 673 875 1342 2094
Size: 212 131 183 27 120 202 467 752
Fragments arranged by size:
752 467 212 202 183 131 120 27
AflII C'TTAA_G
Cuts at: 0 814 2094
Size: 814 1280
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 803 2094
Size: 803 1291
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1352 1384 2094
Size: 1352 32 710
Fragments arranged by size:
1352 710 32
AlwNI CAG_nnn'CTG
Cuts at: 0 783 995 1176 1416 2094
Size: 783 212 181 240 678
Fragments arranged by size:
783 678 240 212 181
ApaI G_GGCC'C
Cuts at: 0 741 1108 2094
Size: 741 367 986
Fragments arranged by size:
986 741 367
ApoI r'AATT_y
Cuts at: 0 547 1447 2094
Size: 547 900 647
Fragments arranged by size:
900 647 547
AvaI C'yCGr_G
Cuts at: 0 842 2094
Size: 842 1252
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 346 379 2094
Size: 346 33 1715
Fragments arranged by size:
1715 346 33
BanI G'GyrC_C
Cuts at: 0 1166 2094
Size: 1166 928
BanII G_rGCy'C
Cuts at: 0 583 741 942 1088 1108 1535 1838 1999
Size: 583 158 201 146 20 427 303 161
Cuts at: 1999 2094
Size: 95
Fragments arranged by size:
583 427 303 201 161 158 146 95
20
BbsI GAAGACnn'nnnn_
Cuts at: 0 392 1007 1133 1640 2094
Size: 392 615 126 507 454
Fragments arranged by size:
615 507 454 392 126
BbvCI CC'TCA_GC
Cuts at: 0 926 2094
Size: 926 1168
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 811 845 2094
Size: 811 34 1249
Fragments arranged by size:
1249 811 34
BglII A'GATC_T
Cuts at: 0 1328 1820 2094
Size: 1328 492 274
Fragments arranged by size:
1328 492 274
BlpI GC'TnA_GC
Cuts at: 0 620 772 932 2094
Size: 620 152 160 1162
Fragments arranged by size:
1162 620 160 152
Bme1580I G_kGCm'C
Cuts at: 0 741 1108 2094
Size: 741 367 986
Fragments arranged by size:
986 741 367
BmgBI CAC'GTC
Cuts at: 0 165 2094
Size: 165 1929
BmrI ACTGGGnnnn_n'
Cuts at: 0 1279 2094
Size: 1279 815
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 597 2094
Size: 597 1497
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 567 1117 1249 1897 2094
Size: 567 550 132 648 197
Fragments arranged by size:
648 567 550 197 132
Bpu10I CC'TnA_GC
Cuts at: 0 926 1983 2094
Size: 926 1057 111
Fragments arranged by size:
1057 926 111
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1996 2094
Size: 1996 98
BsaI GGTCTCn'nnnn_
Cuts at: 0 19 2094
Size: 19 2075
BsaWI w'CCGG_w
Cuts at: 0 1748 2094
Size: 1748 346
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1825 1855 2094
Size: 1825 30 239
Fragments arranged by size:
1825 239 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 469 772 1023 1480 1852 2094
Size: 469 303 251 457 372 242
Fragments arranged by size:
469 457 372 303 251 242
BseYI C'CCAG_C
Cuts at: 0 20 288 335 1089 1096 2094
Size: 20 268 47 754 7 998
Fragments arranged by size:
998 754 268 47 20 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 879 2094
Size: 879 1215
BsiHKAI G_wGCw'C
Cuts at: 0 164 583 626 942 1535 2094
Size: 164 419 43 316 593 559
Fragments arranged by size:
593 559 419 316 164 43
BsmI GAATG_Cn'
Cuts at: 0 1495 1896 2094
Size: 1495 401 198
Fragments arranged by size:
1495 401 198
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1729 2094
Size: 1729 365
Bsp1286I G_dGCh'C
Cuts at: 0 164 583 626 741 942 1088 1108 1535
Size: 164 419 43 115 201 146 20 427
Cuts at: 1535 1838 1999 2094
Size: 303 161 95
Fragments arranged by size:
427 419 303 201 164 161 146 115
95 43 20
BspHI T'CATG_A
Cuts at: 0 1898 2094
Size: 1898 196
BsrBI CCG'CTC
Cuts at: 0 702 2094
Size: 702 1392
BsrDI GCAATG_nn'
Cuts at: 0 1304 1502 2094
Size: 1304 198 592
Fragments arranged by size:
1304 592 198
BstAPI GCAn_nnn'nTGC
Cuts at: 0 277 2094
Size: 277 1817
BstEII G'GTnAC_C
Cuts at: 0 1654 2094
Size: 1654 440
BstYI r'GATC_y
Cuts at: 0 725 1328 1340 1523 1820 2094
Size: 725 603 12 183 297 274
Fragments arranged by size:
725 603 297 274 183 12
Bsu36I CC'TnA_GG
Cuts at: 0 439 2094
Size: 439 1655
BtsI GCAGTG_nn'
Cuts at: 0 1168 1201 2094
Size: 1168 33 893
Fragments arranged by size:
1168 893 33
DrdI GACnn_nn'nnGTC
Cuts at: 0 2053 2094
Size: 2053 41
EaeI y'GGCC_r
Cuts at: 0 1753 2094
Size: 1753 341
EarI CTCTTCn'nnn_
Cuts at: 0 128 571 894 1019 2094
Size: 128 443 323 125 1075
Fragments arranged by size:
1075 443 323 128 125
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 718 2094
Size: 718 1376
EcoICRI GAG'CTC
Cuts at: 0 581 940 1533 2094
Size: 581 359 593 561
Fragments arranged by size:
593 581 561 359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 343 526 553 567 673 875 1117
Size: 212 131 183 27 14 106 202 242
Cuts at: 1117 1249 1342 1897 2094
Size: 132 93 555 197
Fragments arranged by size:
555 242 212 202 197 183 132 131
106 93 27 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 814 1267 2094
Size: 814 453 827
Fragments arranged by size:
827 814 453
EcoO109I rG'GnC_Cy
Cuts at: 0 36 435 738 957 1104 1215 1551 2094
Size: 36 399 303 219 147 111 336 543
Fragments arranged by size:
543 399 336 303 219 147 111 36
EcoRI G'AATT_C
Cuts at: 0 547 2094
Size: 547 1547
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 79 829 1347 2037 2094
Size: 79 750 518 690 57
Fragments arranged by size:
750 690 518 79 57
HaeII r_GCGC'y
Cuts at: 0 1386 1809 2091 2094
Size: 1386 423 282 3
Fragments arranged by size:
1386 423 282 3
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 229 259 261 291 478 510 888 920
Size: 229 30 2 30 187 32 378 32
Cuts at: 920 1707 1739 1767 1799 1825 1857 2094
Size: 787 32 28 32 26 32 237
Fragments arranged by size:
787 378 237 229 187 32 32 32
32 32 30 30 28 26 2
HincII GTy'rAC
Cuts at: 0 822 1205 2094
Size: 822 383 889
Fragments arranged by size:
889 822 383
KpnI G_GTAC'C
Cuts at: 0 1170 2094
Size: 1170 924
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 74 2094
Size: 74 2020
MscI TGG'CCA
Cuts at: 0 1755 2094
Size: 1755 339
MslI CAynn'nnrTG
Cuts at: 0 1156 1285 1784 2094
Size: 1156 129 499 310
Fragments arranged by size:
1156 499 310 129
MspA1I CmG'CkG
Cuts at: 0 783 855 1176 2094
Size: 783 72 321 918
Fragments arranged by size:
918 783 321 72
NdeI CA'TA_TG
Cuts at: 0 176 1758 2094
Size: 176 1582 336
Fragments arranged by size:
1582 336 176
NspI r_CATG'y
Cuts at: 0 1710 2094
Size: 1710 384
PflMI CCAn_nnn'nTGG
Cuts at: 0 783 2094
Size: 783 1311
PfoI T'CCnGG_A
Cuts at: 0 551 1519 1874 2094
Size: 551 968 355 220
Fragments arranged by size:
968 551 355 220
PpuMI rG'GwC_Cy
Cuts at: 0 957 1551 2094
Size: 957 594 543
Fragments arranged by size:
957 594 543
PshAI GACnn'nnGTC
Cuts at: 0 56 2094
Size: 56 2038
PspOMI G'GGCC_C
Cuts at: 0 737 1104 2094
Size: 737 367 990
Fragments arranged by size:
990 737 367
PstI C_TGCA'G
Cuts at: 0 755 770 1181 2094
Size: 755 15 411 913
Fragments arranged by size:
913 755 411 15
PvuII CAG'CTG
Cuts at: 0 783 855 1176 2094
Size: 783 72 321 918
Fragments arranged by size:
918 783 321 72
SacI G_AGCT'C
Cuts at: 0 583 942 1535 2094
Size: 583 359 593 559
Fragments arranged by size:
593 583 559 359
SalI G'TCGA_C
Cuts at: 0 820 2094
Size: 820 1274
SapI GCTCTTCn'nnn_
Cuts at: 0 571 2094
Size: 571 1523
SexAI A'CCwGG_T
Cuts at: 0 1910 2094
Size: 1910 184
SfcI C'TryA_G
Cuts at: 0 751 766 1177 2094
Size: 751 15 411 917
Fragments arranged by size:
917 751 411 15
SmlI C'TyrA_G
Cuts at: 0 814 2011 2094
Size: 814 1197 83
Fragments arranged by size:
1197 814 83
StyI C'CwwG_G
Cuts at: 0 317 593 2060 2094
Size: 317 276 1467 34
Fragments arranged by size:
1467 317 276 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 141 2094
Size: 141 1953
Tth111I GACn'n_nGTC
Cuts at: 0 391 2094
Size: 391 1703
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 591 1509 2094
Size: 591 918 585
Fragments arranged by size:
918 591 585
Enzymes that do cut:
AccI Acc65I AcuI AflII AlfI AloI AlwNI ApaI
ApoI AvaI BaeI BanI BanII BbsI BbvCI BcgI
BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BsrBI BsrDI BstAPI BstEII
BstYI Bsu36I BtsI DrdI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI FalI HaeII Hin4I HincII
KpnI MmeI MscI MslI MspA1I NdeI NspI PflMI
PfoI PpuMI PshAI PspOMI PstI PvuII SacI SalI
SapI SexAI SfcI SmlI StyI TaqII Tth111I XcmI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI AleI
ApaLI AscI AseI AsiSI AvrII BamHI BbeI BciVI
BclI BfrBI BglI BmtI BsaAI BsaBI BsaHI BsiEI
BsiWI BspEI BspMI BsrFI BsrGI BssHII BssSI BstBI
BstXI BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII
EagI EcoRV FseI FspI FspAI HindIII HpaI KasI
MfeI MluI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PsiI PspXI PsrI PvuI RsrII SacII SanDI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI TstI XbaI
XhoI XmaI XmnI ZraI