Restriction map for
pFN21AE6724
(Linear) (Six-base) MAPSORT of: KIEE6724.seq Check: 1683 from: 1 to: 2079
KIEE6724.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AccI GT'mk_AC
Cuts at: 0 470 740 1944 2079
Size: 470 270 1204 135
Fragments arranged by size:
1204 470 270 135
AclI AA'CG_TT
Cuts at: 0 976 1667 2079
Size: 976 691 412
Fragments arranged by size:
976 691 412
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 269 412 593 628 1019 1370 2079
Size: 269 143 181 35 391 351 709
Fragments arranged by size:
709 391 351 269 181 143 35
AflII C'TTAA_G
Cuts at: 0 49 2079
Size: 49 2030
AflIII A'CryG_T
Cuts at: 0 406 1583 2079
Size: 406 1177 496
Fragments arranged by size:
1177 496 406
AhdI GACnn_n'nnGTC
Cuts at: 0 992 2079
Size: 992 1087
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 188 220 2079
Size: 188 32 1859
Fragments arranged by size:
1859 188 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1321 2079
Size: 1321 758
ApaLI G'TGCA_C
Cuts at: 0 352 802 2079
Size: 352 450 1277
Fragments arranged by size:
1277 450 352
ApoI r'AATT_y
Cuts at: 0 158 305 612 872 1259 2079
Size: 158 147 307 260 387 820
Fragments arranged by size:
820 387 307 260 158 147
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1016 1049 2079
Size: 1016 33 1030
Fragments arranged by size:
1030 1016 33
BanII G_rGCy'C
Cuts at: 0 1190 2079
Size: 1190 889
BciVI GTATCCnnnnn_n'
Cuts at: 0 1017 2079
Size: 1017 1062
BglII A'GATC_T
Cuts at: 0 1897 2079
Size: 1897 182
Bme1580I G_kGCm'C
Cuts at: 0 356 806 2079
Size: 356 450 1273
Fragments arranged by size:
1273 450 356
BmgBI CAC'GTC
Cuts at: 0 707 2079
Size: 707 1372
BmrI ACTGGGnnnn_n'
Cuts at: 0 1626 2079
Size: 1626 453
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 59 245 1581 2079
Size: 49 10 186 1336 498
Fragments arranged by size:
1336 498 186 49 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 2079
Size: 691 1388
BsaI GGTCTCn'nnnn_
Cuts at: 0 38 2079
Size: 38 2041
BsaAI yAC'GTr
Cuts at: 0 1584 2079
Size: 1584 495
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 4 34 68 98 335 365 452 482
Size: 4 30 34 30 237 30 87 30
Cuts at: 482 1792 1822 2079
Size: 1310 30 257
Fragments arranged by size:
1310 257 237 87 34 30 30 30
30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 56 1093 1222 2079
Size: 56 1037 129 857
Fragments arranged by size:
1037 857 129 56
BseYI C'CCAG_C
Cuts at: 0 1813 2079
Size: 1813 266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 169 676 1449 1576 2079
Size: 169 507 773 127 503
Fragments arranged by size:
773 507 503 169 127
BsiHKAI G_wGCw'C
Cuts at: 0 105 356 561 806 1190 1754 2079
Size: 105 251 205 245 384 564 325
Fragments arranged by size:
564 384 325 251 245 205 105
BsmI GAATG_Cn'
Cuts at: 0 39 1512 1527 2079
Size: 39 1473 15 552
Fragments arranged by size:
1473 552 39 15
Bsp1286I G_dGCh'C
Cuts at: 0 105 356 561 806 1190 1754 2079
Size: 105 251 205 245 384 564 325
Fragments arranged by size:
564 384 325 251 245 205 105
BspHI T'CATG_A
Cuts at: 0 1934 2079
Size: 1934 145
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1956 2079
Size: 1956 123
BsrDI GCAATG_nn'
Cuts at: 0 794 1968 2079
Size: 794 1174 111
Fragments arranged by size:
1174 794 111
BsrFI r'CCGG_y
Cuts at: 0 1833 2079
Size: 1833 246
BsrGI T'GTAC_A
Cuts at: 0 754 2079
Size: 754 1325
BstBI TT'CG_AA
Cuts at: 0 1911 2079
Size: 1911 168
BstYI r'GATC_y
Cuts at: 0 1897 1967 2079
Size: 1897 70 112
Fragments arranged by size:
1897 112 70
BstZ17I GTA'TAC
Cuts at: 0 1945 2079
Size: 1945 134
BtgI C'CryG_G
Cuts at: 0 955 2079
Size: 955 1124
BtsI GCAGTG_nn'
Cuts at: 0 645 2079
Size: 645 1434
EaeI y'GGCC_r
Cuts at: 0 57 2079
Size: 57 2022
EarI CTCTTCn'nnn_
Cuts at: 0 123 845 995 1283 1469 2079
Size: 123 722 150 288 186 610
Fragments arranged by size:
722 610 288 186 150 123
EcoICRI GAG'CTC
Cuts at: 0 1188 2079
Size: 1188 891
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 59 245 269 412 593 628 1019
Size: 49 10 186 24 143 181 35 391
Cuts at: 1019 1370 1581 2079
Size: 351 211 498
Fragments arranged by size:
498 391 351 211 186 181 143 49
35 24 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 430 2079
Size: 430 1649
EcoO109I rG'GnC_Cy
Cuts at: 0 229 423 855 1318 1866 2079
Size: 229 194 432 463 548 213
Fragments arranged by size:
548 463 432 229 213 194
EcoRI G'AATT_C
Cuts at: 0 872 2079
Size: 872 1207
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 598 2079
Size: 598 1481
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 68 100 978 1010 1606 1638 2032 2064
Size: 68 32 878 32 596 32 394 32
Cuts at: 2064 2079
Size: 15
Fragments arranged by size:
878 596 394 68 32 32 32 32
15
HincII GTy'rAC
Cuts at: 0 1671 2079
Size: 1671 408
HindIII A'AGCT_T
Cuts at: 0 865 2079
Size: 865 1214
HpaI GTT'AAC
Cuts at: 0 1671 2079
Size: 1671 408
MscI TGG'CCA
Cuts at: 0 59 2079
Size: 59 2020
MslI CAynn'nnrTG
Cuts at: 0 68 394 799 1175 1247 2079
Size: 68 326 405 376 72 832
Fragments arranged by size:
832 405 376 326 72 68
NspI r_CATG'y
Cuts at: 0 410 1659 2079
Size: 410 1249 420
Fragments arranged by size:
1249 420 410
PciI A'CATG_T
Cuts at: 0 406 2079
Size: 406 1673
PflMI CCAn_nnn'nTGG
Cuts at: 0 1321 2079
Size: 1321 758
PpuMI rG'GwC_Cy
Cuts at: 0 229 1318 1866 2079
Size: 229 1089 548 213
Fragments arranged by size:
1089 548 229 213
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1905 1937 2079
Size: 1905 32 142
Fragments arranged by size:
1905 142 32
SacI G_AGCT'C
Cuts at: 0 1190 2079
Size: 1190 889
SanDI GG'GwC_CC
Cuts at: 0 1866 2079
Size: 1866 213
ScaI AGT'ACT
Cuts at: 0 1758 2079
Size: 1758 321
SfcI C'TryA_G
Cuts at: 0 1547 1575 1715 1973 2079
Size: 1547 28 140 258 106
Fragments arranged by size:
1547 258 140 106 28
SmlI C'TyrA_G
Cuts at: 0 49 670 2079
Size: 49 621 1409
Fragments arranged by size:
1409 621 49
SpeI A'CTAG_T
Cuts at: 0 1924 2079
Size: 1924 155
SspI AAT'ATT
Cuts at: 0 278 1392 2079
Size: 278 1114 687
Fragments arranged by size:
1114 687 278
StyI C'CwwG_G
Cuts at: 0 167 1095 2079
Size: 167 928 984
Fragments arranged by size:
984 928 167
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 140 2079
Size: 140 1939
TatI w'GTAC_w
Cuts at: 0 754 1756 2079
Size: 754 1002 323
Fragments arranged by size:
1002 754 323
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 335 367 2079
Size: 335 32 1712
Fragments arranged by size:
1712 335 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 574 2079
Size: 574 1505
XmnI GAAnn'nnTTC
Cuts at: 0 613 1525 2079
Size: 613 912 554
Fragments arranged by size:
912 613 554
Enzymes that do cut:
AccI AclI AcuI AflII AflIII AhdI AloI AlwNI
ApaLI ApoI BaeI BanII BciVI BglII Bme1580I BmgBI
BmrI BpmI BpuEI BsaI BsaAI BsaXI BseRI BseYI
BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI
BsrGI BstBI BstYI BstZ17I BtgI BtsI EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI Hin4I HincII
HindIII HpaI MscI MslI NspI PciI PflMI PpuMI
PsrI SacI SanDI ScaI SfcI SmlI SpeI SspI
StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AgeI AleI AlfI ApaI
AscI AseI AsiSI AvaI AvrII BamHI BanI BbeI
BbsI BbvCI BcgI BclI BfrBI BglI BlpI BmtI
BplI Bpu10I BsaBI BsaHI BsaWI BsiEI BsiWI BsmBI
BspEI BsrBI BssHII BssSI BstAPI BstEII BstXI Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoRV FseI FspI FspAI HaeII KasI KpnI MfeI
MluI MmeI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PstI PvuI
PvuII RsrII SacII SalI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StuI
SwaI Tth111I XbaI XhoI XmaI ZraI