Restriction map for
pFN21AE6720
(Linear) (Six-base) MAPSORT of: KIEE6720.seq Check: 7671 from: 1 to: 2259
KIEE6720.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AccI GT'mk_AC
Cuts at: 0 497 1352 2259
Size: 497 855 907
Fragments arranged by size:
907 855 497
Acc65I G'GTAC_C
Cuts at: 0 1210 1653 2065 2259
Size: 1210 443 412 194
Fragments arranged by size:
1210 443 412 194
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1375 1952 2259
Size: 1375 577 307
Fragments arranged by size:
1375 577 307
AflIII A'CryG_T
Cuts at: 0 283 2259
Size: 283 1976
AleI CACnn'nnGTG
Cuts at: 0 1543 2259
Size: 1543 716
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 314 2152 2259
Size: 314 1838 107
Fragments arranged by size:
1838 314 107
ApoI r'AATT_y
Cuts at: 0 660 2259
Size: 660 1599
AvaI C'yCGr_G
Cuts at: 0 155 2259
Size: 155 2104
BanI G'GyrC_C
Cuts at: 0 139 871 1210 1653 1948 2065 2259
Size: 139 732 339 443 295 117 194
Fragments arranged by size:
732 443 339 295 194 139 117
BbeI G_GCGC'C
Cuts at: 0 143 2259
Size: 143 2116
BbsI GAAGACnn'nnnn_
Cuts at: 0 1090 2259
Size: 1090 1169
BfrBI ATG'CAT
Cuts at: 0 18 2259
Size: 18 2241
BglI GCCn_nnn'nGGC
Cuts at: 0 43 2259
Size: 43 2216
BglII A'GATC_T
Cuts at: 0 405 1227 2259
Size: 405 822 1032
Fragments arranged by size:
1032 822 405
Bme1580I G_kGCm'C
Cuts at: 0 876 2259
Size: 876 1383
BmrI ACTGGGnnnn_n'
Cuts at: 0 870 1879 2259
Size: 870 1009 380
Fragments arranged by size:
1009 870 380
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 328 2259
Size: 328 1931
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 626 2259
Size: 238 388 1633
Fragments arranged by size:
1633 388 238
Bpu10I CC'TnA_GC
Cuts at: 0 108 727 2259
Size: 108 619 1532
Fragments arranged by size:
1532 619 108
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1330 1807 2259
Size: 1330 477 452
Fragments arranged by size:
1330 477 452
BsaBI GATnn'nnATC
Cuts at: 0 79 2259
Size: 79 2180
BsaHI Gr'CG_yC
Cuts at: 0 140 2259
Size: 140 2119
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1217 1247 2259
Size: 1217 30 1012
Fragments arranged by size:
1217 1012 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 320 1850 2206 2259
Size: 320 1530 356 53
Fragments arranged by size:
1530 356 320 53
BseYI C'CCAG_C
Cuts at: 0 29 432 2259
Size: 29 403 1827
Fragments arranged by size:
1827 403 29
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2158 2259
Size: 2158 101
BsiEI CG_ry'CG
Cuts at: 0 38 2259
Size: 38 2221
BsiHKAI G_wGCw'C
Cuts at: 0 1187 2259
Size: 1187 1072
BsmI GAATG_Cn'
Cuts at: 0 18 919 1787 2259
Size: 18 901 868 472
Fragments arranged by size:
901 868 472 18
Bsp1286I G_dGCh'C
Cuts at: 0 876 1187 2259
Size: 876 311 1072
Fragments arranged by size:
1072 876 311
BspHI T'CATG_A
Cuts at: 0 1764 2259
Size: 1764 495
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 979 1198 2259
Size: 979 219 1061
Fragments arranged by size:
1061 979 219
BsrBI CCG'CTC
Cuts at: 0 154 2259
Size: 154 2105
BsrDI GCAATG_nn'
Cuts at: 0 377 1988 2259
Size: 377 1611 271
Fragments arranged by size:
1611 377 271
BsrFI r'CCGG_y
Cuts at: 0 62 2259
Size: 62 2197
BstAPI GCAn_nnn'nTGC
Cuts at: 0 994 2259
Size: 994 1265
BstBI TT'CG_AA
Cuts at: 0 53 630 1779 2259
Size: 53 577 1149 480
Fragments arranged by size:
1149 577 480 53
BstEII G'GTnAC_C
Cuts at: 0 888 2259
Size: 888 1371
BstXI CCAn_nnnn'nTGG
Cuts at: 0 432 2259
Size: 432 1827
BstYI r'GATC_y
Cuts at: 0 405 711 1227 2259
Size: 405 306 516 1032
Fragments arranged by size:
1032 516 405 306
Bsu36I CC'TnA_GG
Cuts at: 0 1658 1727 2259
Size: 1658 69 532
Fragments arranged by size:
1658 532 69
BtgI C'CryG_G
Cuts at: 0 38 563 1415 2073 2259
Size: 38 525 852 658 186
Fragments arranged by size:
852 658 525 186 38
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 28 2259
Size: 28 2231
BtsI GCAGTG_nn'
Cuts at: 0 124 1009 1649 1748 2259
Size: 124 885 640 99 511
Fragments arranged by size:
885 640 511 124 99
DraI TTT'AAA
Cuts at: 0 486 2259
Size: 486 1773
DraIII CAC_nnn'GTG
Cuts at: 0 941 2259
Size: 941 1318
DrdI GACnn_nn'nnGTC
Cuts at: 0 1885 2259
Size: 1885 374
EaeI y'GGCC_r
Cuts at: 0 35 262 1505 2259
Size: 35 227 1243 754
Fragments arranged by size:
1243 754 227 35
EagI C'GGCC_G
Cuts at: 0 35 2259
Size: 35 2224
EarI CTCTTCn'nnn_
Cuts at: 0 1678 1928 2259
Size: 1678 250 331
Fragments arranged by size:
1678 331 250
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 626 1375 1952 2259
Size: 238 388 749 577 307
Fragments arranged by size:
749 577 388 307 238
EcoO109I rG'GnC_Cy
Cuts at: 0 1878 2259
Size: 1878 381
EcoRI G'AATT_C
Cuts at: 0 660 2259
Size: 660 1599
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 391 856 2259
Size: 391 465 1403
Fragments arranged by size:
1403 465 391
HaeII r_GCGC'y
Cuts at: 0 7 30 63 143 2259
Size: 7 23 33 80 2116
Fragments arranged by size:
2116 80 33 23 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 114 146 1298 1330 1368 1400 2259
Size: 114 32 1152 32 38 32 859
Fragments arranged by size:
1152 859 114 38 32 32 32
HincII GTy'rAC
Cuts at: 0 2006 2259
Size: 2006 253
HindIII A'AGCT_T
Cuts at: 0 1825 2259
Size: 1825 434
KasI G'GCGC_C
Cuts at: 0 139 2259
Size: 139 2120
KpnI G_GTAC'C
Cuts at: 0 1214 1657 2069 2259
Size: 1214 443 412 190
Fragments arranged by size:
1214 443 412 190
MfeI C'AATT_G
Cuts at: 0 1856 2259
Size: 1856 403
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1203 2259
Size: 1203 1056
MscI TGG'CCA
Cuts at: 0 264 1507 2259
Size: 264 1243 752
Fragments arranged by size:
1243 752 264
MslI CAynn'nnrTG
Cuts at: 0 296 1321 1543 1793 2259
Size: 296 1025 222 250 466
Fragments arranged by size:
1025 466 296 250 222
MspA1I CmG'CkG
Cuts at: 0 40 2259
Size: 40 2219
NaeI GCC'GGC
Cuts at: 0 64 2259
Size: 64 2195
NarI GG'CG_CC
Cuts at: 0 140 2259
Size: 140 2119
NcoI C'CATG_G
Cuts at: 0 563 2073 2259
Size: 563 1510 186
Fragments arranged by size:
1510 563 186
NgoMIV G'CCGG_C
Cuts at: 0 62 2259
Size: 62 2197
NotI GC'GGCC_GC
Cuts at: 0 35 2259
Size: 35 2224
NruI TCG'CGA
Cuts at: 0 13 2259
Size: 13 2246
NsiI A_TGCA'T
Cuts at: 0 20 2259
Size: 20 2239
NspI r_CATG'y
Cuts at: 0 287 528 2259
Size: 287 241 1731
Fragments arranged by size:
1731 287 241
PciI A'CATG_T
Cuts at: 0 283 2259
Size: 283 1976
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1805 1837 2259
Size: 1805 32 422
Fragments arranged by size:
1805 422 32
PpuMI rG'GwC_Cy
Cuts at: 0 1878 2259
Size: 1878 381
PspXI vC'TCGA_Gb
Cuts at: 0 155 2259
Size: 155 2104
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 891 923 2259
Size: 891 32 1336
Fragments arranged by size:
1336 891 32
PstI C_TGCA'G
Cuts at: 0 1494 2259
Size: 1494 765
SacII CC_GC'GG
Cuts at: 0 41 2259
Size: 41 2218
SbfI CC_TGCA'GG
Cuts at: 0 1494 2259
Size: 1494 765
SfcI C'TryA_G
Cuts at: 0 494 1001 1490 2259
Size: 494 507 489 769
Fragments arranged by size:
769 507 494 489
SfoI GGC'GCC
Cuts at: 0 141 2259
Size: 141 2118
SmlI C'TyrA_G
Cuts at: 0 155 1345 1822 2259
Size: 155 1190 477 437
Fragments arranged by size:
1190 477 437 155
SspI AAT'ATT
Cuts at: 0 465 2259
Size: 465 1794
StyI C'CwwG_G
Cuts at: 0 143 563 894 2073 2259
Size: 143 420 331 1179 186
Fragments arranged by size:
1179 420 331 186 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 433 1372 1816 2259
Size: 433 939 444 443
Fragments arranged by size:
939 444 443 433
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1487 1519 2259
Size: 1487 32 740
Fragments arranged by size:
1487 740 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 465 1959 2259
Size: 465 1494 300
Fragments arranged by size:
1494 465 300
XhoI C'TCGA_G
Cuts at: 0 155 2259
Size: 155 2104
XmnI GAAnn'nnTTC
Cuts at: 0 1631 2250 2259
Size: 1631 619 9
Fragments arranged by size:
1631 619 9
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlfI ApoI AvaI
BanI BbeI BbsI BfrBI BglI BglII Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EagI EarI Eco57MI EcoO109I EcoRI FalI HaeII Hin4I
HincII HindIII KasI KpnI MfeI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NotI NruI NsiI
NspI PciI PpiI PpuMI PspXI PsrI PstI SacII
SbfI SfcI SfoI SmlI SspI StyI TaqII TstI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BanII BbvCI BcgI BciVI BclI BlpI BmgBI
BmtI BsaI BsaAI BsaWI BsiWI BsmBI BspEI BsrGI
BssHII BssSI BstZ17I ClaI CspCI EciI EcoICRI EcoNI
EcoRV FseI FspI FspAI HpaI MluI NdeI NheI
PacI PasI PflMI PfoI PmeI PmlI PshAI PsiI
PspOMI PvuI PvuII RsrII SacI SalI SanDI SapI
ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI TatI Tth111I XbaI XmaI
ZraI