Restriction map for
pFN21AE6716
(Linear) (Six-base) MAPSORT of: KIEE6716.seq Check: 3389 from: 1 to: 2265
KIEE6716.orf
With 182 enzymes: SgfI *
March 16, 2015 11:36 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 2062 2066 2265
Size: 735 1327 4 199
Fragments arranged by size:
1327 735 199 4
AhdI GACnn_n'nnGTC
Cuts at: 0 1190 2265
Size: 1190 1075
AleI CACnn'nnGTG
Cuts at: 0 227 547 2265
Size: 227 320 1718
Fragments arranged by size:
1718 320 227
AlwNI CAG_nnn'CTG
Cuts at: 0 421 1168 1246 2135 2265
Size: 421 747 78 889 130
Fragments arranged by size:
889 747 421 130 78
ApoI r'AATT_y
Cuts at: 0 17 372 2071 2265
Size: 17 355 1699 194
Fragments arranged by size:
1699 355 194 17
AseI AT'TA_AT
Cuts at: 0 1763 2265
Size: 1763 502
AvaI C'yCGr_G
Cuts at: 0 2213 2265
Size: 2213 52
AvrII C'CTAG_G
Cuts at: 0 469 2265
Size: 469 1796
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1247 1280 1665 1698 2265
Size: 1247 33 385 33 567
Fragments arranged by size:
1247 567 385 33 33
BbsI GAAGACnn'nnnn_
Cuts at: 0 1610 1897 2210 2265
Size: 1610 287 313 55
Fragments arranged by size:
1610 313 287 55
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2151 2185 2265
Size: 2151 34 80
Fragments arranged by size:
2151 80 34
BlpI GC'TnA_GC
Cuts at: 0 125 2265
Size: 125 2140
BmrI ACTGGGnnnn_n'
Cuts at: 0 77 824 2265
Size: 77 747 1441
Fragments arranged by size:
1441 747 77
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1268 1420 1460 2137 2265
Size: 1268 152 40 677 128
Fragments arranged by size:
1268 677 152 128 40
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1327 2265
Size: 1327 938
BsaI GGTCTCn'nnnn_
Cuts at: 0 823 1946 2265
Size: 823 1123 319
Fragments arranged by size:
1123 823 319
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 2132 2162 2265
Size: 2132 30 103
Fragments arranged by size:
2132 103 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 309 674 1342 2265
Size: 309 365 668 923
Fragments arranged by size:
923 668 365 309
BseYI C'CCAG_C
Cuts at: 0 129 743 788 797 2265
Size: 129 614 45 9 1468
Fragments arranged by size:
1468 614 129 45 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1437 2265
Size: 1437 828
BsiHKAI G_wGCw'C
Cuts at: 0 614 2153 2265
Size: 614 1539 112
Fragments arranged by size:
1539 614 112
BsmI GAATG_Cn'
Cuts at: 0 113 1027 1352 1997 2265
Size: 113 914 325 645 268
Fragments arranged by size:
914 645 325 268 113
Bsp1286I G_dGCh'C
Cuts at: 0 614 2153 2265
Size: 614 1539 112
Fragments arranged by size:
1539 614 112
BspHI T'CATG_A
Cuts at: 0 1116 2265
Size: 1116 1149
BsrFI r'CCGG_y
Cuts at: 0 2252 2265
Size: 2252 13
BsrGI T'GTAC_A
Cuts at: 0 1102 2265
Size: 1102 1163
BstAPI GCAn_nnn'nTGC
Cuts at: 0 770 2265
Size: 770 1495
BstEII G'GTnAC_C
Cuts at: 0 552 1842 2265
Size: 552 1290 423
Fragments arranged by size:
1290 552 423
BstXI CCAn_nnnn'nTGG
Cuts at: 0 146 622 1045 2265
Size: 146 476 423 1220
Fragments arranged by size:
1220 476 423 146
BstYI r'GATC_y
Cuts at: 0 739 1336 2265
Size: 739 597 929
Fragments arranged by size:
929 739 597
BtsI GCAGTG_nn'
Cuts at: 0 942 1056 1425 2265
Size: 942 114 369 840
Fragments arranged by size:
942 840 369 114
DraI TTT'AAA
Cuts at: 0 1896 2265
Size: 1896 369
DraIII CAC_nnn'GTG
Cuts at: 0 1561 2265
Size: 1561 704
DrdI GACnn_nn'nnGTC
Cuts at: 0 2140 2265
Size: 2140 125
EarI CTCTTCn'nnn_
Cuts at: 0 194 2218 2265
Size: 194 2024 47
Fragments arranged by size:
2024 194 47
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 735 1268 1420 1460 2062 2066 2137 2265
Size: 735 533 152 40 602 4 71 128
Fragments arranged by size:
735 602 533 152 128 71 40 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1522 2265
Size: 1522 743
EcoO109I rG'GnC_Cy
Cuts at: 0 628 2265
Size: 628 1637
EcoRI G'AATT_C
Cuts at: 0 372 2265
Size: 372 1893
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1591 2265
Size: 1591 674
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 415 447 510 542 691 723 945 977
Size: 415 32 63 32 149 32 222 32
Cuts at: 977 1060 1092 1176 1208 1321 1353 2265
Size: 83 32 84 32 113 32 912
Fragments arranged by size:
912 415 222 149 113 84 83 63
32 32 32 32 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 675 2265
Size: 675 1590
MslI CAynn'nnrTG
Cuts at: 0 227 547 620 1765 1951 2265
Size: 227 320 73 1145 186 314
Fragments arranged by size:
1145 320 314 227 186 73
MspA1I CmG'CkG
Cuts at: 0 1494 1839 2265
Size: 1494 345 426
Fragments arranged by size:
1494 426 345
NaeI GCC'GGC
Cuts at: 0 2254 2265
Size: 2254 11
NgoMIV G'CCGG_C
Cuts at: 0 2252 2265
Size: 2252 13
NspI r_CATG'y
Cuts at: 0 1704 2211 2265
Size: 1704 507 54
Fragments arranged by size:
1704 507 54
PpuMI rG'GwC_Cy
Cuts at: 0 628 2265
Size: 628 1637
PstI C_TGCA'G
Cuts at: 0 394 2265
Size: 394 1871
PvuII CAG'CTG
Cuts at: 0 1494 1839 2265
Size: 1494 345 426
Fragments arranged by size:
1494 426 345
SanDI GG'GwC_CC
Cuts at: 0 628 2265
Size: 628 1637
SapI GCTCTTCn'nnn_
Cuts at: 0 194 2265
Size: 194 2071
ScaI AGT'ACT
Cuts at: 0 270 2158 2265
Size: 270 1888 107
Fragments arranged by size:
1888 270 107
SexAI A'CCwGG_T
Cuts at: 0 1754 2265
Size: 1754 511
SfcI C'TryA_G
Cuts at: 0 390 2265
Size: 390 1875
SmlI C'TyrA_G
Cuts at: 0 1342 2265
Size: 1342 923
StyI C'CwwG_G
Cuts at: 0 469 600 1409 1575 1992 2265
Size: 469 131 809 166 417 273
Fragments arranged by size:
809 469 417 273 166 131
TatI w'GTAC_w
Cuts at: 0 268 1102 2156 2265
Size: 268 834 1054 109
Fragments arranged by size:
1054 834 268 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 298 330 2132 2164 2265
Size: 298 32 1802 32 101
Fragments arranged by size:
1802 298 101 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1756 2265
Size: 1756 509
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 246 2265
Size: 246 2019
XmnI GAAnn'nnTTC
Cuts at: 0 1974 2075 2265
Size: 1974 101 190
Fragments arranged by size:
1974 190 101
Enzymes that do cut:
AcuI AhdI AleI AlwNI ApoI AseI AvaI AvrII
BaeI BbsI BcgI BlpI BmrI BpmI BpuEI BsaI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI
BsrFI BsrGI BstAPI BstEII BstXI BstYI BtsI DraI
DraIII DrdI EarI Eco57MI EcoNI EcoO109I EcoRI FalI
Hin4I MmeI MslI MspA1I NaeI NgoMIV NspI PpuMI
PstI PvuII SanDI SapI ScaI SexAI SfcI SmlI
StyI TatI TstI Tth111I XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AlfI AloI ApaI ApaLI AscI AsiSI BamHI
BanI BanII BbeI BbvCI BciVI BclI BfrBI BglI
BglII Bme1580I BmgBI BmtI BplI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsiEI BsiWI BsmBI BspEI BspMI BsrBI
BsrDI BssHII BssSI BstBI BstZ17I Bsu36I BtgI BtgZI
ClaI CspCI EaeI EagI EciI EcoICRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SbfI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII XbaI XhoI XmaI ZraI