Restriction map for
pFN21AE6712
(Linear) (Six-base) MAPSORT of: KIEE6712.seq Check: 7829 from: 1 to: 2802
KIEE6712.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2385 2746 2802
Size: 2385 361 56
Fragments arranged by size:
2385 361 56
AccI GT'mk_AC
Cuts at: 0 2690 2802
Size: 2690 112
Acc65I G'GTAC_C
Cuts at: 0 1747 2802
Size: 1747 1055
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2033 2338 2802
Size: 2033 305 464
Fragments arranged by size:
2033 464 305
AleI CACnn'nnGTG
Cuts at: 0 784 1066 2802
Size: 784 282 1736
Fragments arranged by size:
1736 784 282
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 70 102 2802
Size: 70 32 2700
Fragments arranged by size:
2700 70 32
ApaI G_GGCC'C
Cuts at: 0 2219 2802
Size: 2219 583
ApoI r'AATT_y
Cuts at: 0 1594 2802
Size: 1594 1208
AvaI C'yCGr_G
Cuts at: 0 466 1084 1286 1414 1742 2018 2048 2802
Size: 466 618 202 128 328 276 30 754
Fragments arranged by size:
754 618 466 328 276 202 128 30
BamHI G'GATC_C
Cuts at: 0 1834 2802
Size: 1834 968
BanI G'GyrC_C
Cuts at: 0 70 982 1627 1747 2479 2512 2611 2617
Size: 70 912 645 120 732 33 99 6
Cuts at: 2617 2802
Size: 185
Fragments arranged by size:
912 732 645 185 120 99 70 33
6
BanII G_rGCy'C
Cuts at: 0 1422 1726 2187 2197 2219 2596 2802
Size: 1422 304 461 10 22 377 206
Fragments arranged by size:
1422 461 377 304 206 22 10
BbeI G_GCGC'C
Cuts at: 0 986 2802
Size: 986 1816
BbsI GAAGACnn'nnnn_
Cuts at: 0 720 2802
Size: 720 2082
BbvCI CC'TCA_GC
Cuts at: 0 1273 1752 2802
Size: 1273 479 1050
Fragments arranged by size:
1273 1050 479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 132 166 1450 1484 2802
Size: 132 34 1284 34 1318
Fragments arranged by size:
1318 1284 132 34 34
BclI T'GATC_A
Cuts at: 0 1940 2802
Size: 1940 862
BlpI GC'TnA_GC
Cuts at: 0 520 2802
Size: 520 2282
Bme1580I G_kGCm'C
Cuts at: 0 1571 1630 1805 2219 2672 2802
Size: 1571 59 175 414 453 130
Fragments arranged by size:
1571 453 414 175 130 59
BmrI ACTGGGnnnn_n'
Cuts at: 0 1072 2643 2802
Size: 1072 1571 159
Fragments arranged by size:
1571 1072 159
BmtI G_CTAG'C
Cuts at: 0 218 2802
Size: 218 2584
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2049 2435 2802
Size: 2049 386 367
Fragments arranged by size:
2049 386 367
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 230 343 367 613 781 2802
Size: 36 194 113 24 246 168 2021
Fragments arranged by size:
2021 246 194 168 113 36 24
Bpu10I CC'TnA_GC
Cuts at: 0 1273 1752 2802
Size: 1273 479 1050
Fragments arranged by size:
1273 1050 479
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1192 2802
Size: 1192 1610
BsaI GGTCTCn'nnnn_
Cuts at: 0 238 1186 2165 2466 2802
Size: 238 948 979 301 336
Fragments arranged by size:
979 948 336 301 238
BsaAI yAC'GTr
Cuts at: 0 2667 2802
Size: 2667 135
BsaBI GATnn'nnATC
Cuts at: 0 961 2802
Size: 961 1841
BsaHI Gr'CG_yC
Cuts at: 0 983 1292 1454 2234 2566 2802
Size: 983 309 162 780 332 236
Fragments arranged by size:
983 780 332 309 236 162
BsaWI w'CCGG_w
Cuts at: 0 247 2379 2802
Size: 247 2132 423
Fragments arranged by size:
2132 423 247
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 574 604 1166 1196 2802
Size: 574 30 562 30 1606
Fragments arranged by size:
1606 574 562 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 33 298 2802
Size: 33 265 2504
Fragments arranged by size:
2504 265 33
BseYI C'CCAG_C
Cuts at: 0 663 1784 2751 2802
Size: 663 1121 967 51
Fragments arranged by size:
1121 967 663 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 1256 1852 2351 2426 2802
Size: 571 685 596 499 75 376
Fragments arranged by size:
685 596 571 499 376 75
BsiEI CG_ry'CG
Cuts at: 0 270 2802
Size: 270 2532
BsiHKAI G_wGCw'C
Cuts at: 0 1002 1726 2197 2596 2802
Size: 1002 724 471 399 206
Fragments arranged by size:
1002 724 471 399 206
BsmI GAATG_Cn'
Cuts at: 0 1470 2802
Size: 1470 1332
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1283 2802
Size: 1283 1519
Bsp1286I G_dGCh'C
Cuts at: 0 1002 1422 1571 1630 1726 1805 2187 2197
Size: 1002 420 149 59 96 79 382 10
Cuts at: 2197 2219 2596 2672 2802
Size: 22 377 76 130
Fragments arranged by size:
1002 420 382 377 149 130 96 79
76 59 22 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 182 1171 2385 2746 2802
Size: 182 989 1214 361 56
Fragments arranged by size:
1214 989 361 182 56
BsrBI CCG'CTC
Cuts at: 0 23 465 2802
Size: 23 442 2337
Fragments arranged by size:
2337 442 23
BsrDI GCAATG_nn'
Cuts at: 0 1002 1686 2712 2769 2802
Size: 1002 684 1026 57 33
Fragments arranged by size:
1026 1002 684 57 33
BsrFI r'CCGG_y
Cuts at: 0 865 2509 2614 2802
Size: 865 1644 105 188
Fragments arranged by size:
1644 865 188 105
BsrGI T'GTAC_A
Cuts at: 0 1070 2802
Size: 1070 1732
BssHII G'CGCG_C
Cuts at: 0 473 597 751 2802
Size: 473 124 154 2051
Fragments arranged by size:
2051 473 154 124
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2667 2802
Size: 2667 135
BstEII G'GTnAC_C
Cuts at: 0 1158 2802
Size: 1158 1644
BstXI CCAn_nnnn'nTGG
Cuts at: 0 14 2343 2562 2802
Size: 14 2329 219 240
Fragments arranged by size:
2329 240 219 14
BstYI r'GATC_y
Cuts at: 0 107 168 807 1834 2802
Size: 107 61 639 1027 968
Fragments arranged by size:
1027 968 639 107 61
BtgI C'CryG_G
Cuts at: 0 89 440 2288 2621 2802
Size: 89 351 1848 333 181
Fragments arranged by size:
1848 351 333 181 89
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1095 1634 2516 2802
Size: 1095 539 882 286
Fragments arranged by size:
1095 882 539 286
BtsI GCAGTG_nn'
Cuts at: 0 298 1973 2802
Size: 298 1675 829
Fragments arranged by size:
1675 829 298
DraIII CAC_nnn'GTG
Cuts at: 0 1767 2802
Size: 1767 1035
EaeI y'GGCC_r
Cuts at: 0 1711 1758 2345 2802
Size: 1711 47 587 457
Fragments arranged by size:
1711 587 457 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 663 2559 2802
Size: 663 1896 243
Fragments arranged by size:
1896 663 243
EcoICRI GAG'CTC
Cuts at: 0 1724 2195 2594 2802
Size: 1724 471 399 208
Fragments arranged by size:
1724 471 399 208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 36 230 343 367 613 781 2033 2338
Size: 36 194 113 24 246 168 1252 305
Cuts at: 2338 2802
Size: 464
Fragments arranged by size:
1252 464 305 246 194 168 113 36
24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1712 2802
Size: 1712 1090
EcoO109I rG'GnC_Cy
Cuts at: 0 659 820 1446 1698 2215 2216 2748 2802
Size: 659 161 626 252 517 1 532 54
Fragments arranged by size:
659 626 532 517 252 161 54 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 518 1233 2802
Size: 518 715 1569
Fragments arranged by size:
1569 715 518
HaeII r_GCGC'y
Cuts at: 0 986 2417 2636 2802
Size: 986 1431 219 166
Fragments arranged by size:
1431 986 219 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 651 683 757 789 1725 1757 2361 2393
Size: 651 32 74 32 936 32 604 32
Cuts at: 2393 2578 2610 2802
Size: 185 32 192
Fragments arranged by size:
936 651 604 192 185 74 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 982 2802
Size: 982 1820
KpnI G_GTAC'C
Cuts at: 0 1751 2802
Size: 1751 1051
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 572 629 1367 2802
Size: 572 57 738 1435
Fragments arranged by size:
1435 738 572 57
MscI TGG'CCA
Cuts at: 0 1760 2347 2802
Size: 1760 587 455
Fragments arranged by size:
1760 587 455
MslI CAynn'nnrTG
Cuts at: 0 697 784 1066 1654 2542 2802
Size: 697 87 282 588 888 260
Fragments arranged by size:
888 697 588 282 260 87
MspA1I CmG'CkG
Cuts at: 0 345 1756 2313 2486 2728 2802
Size: 345 1411 557 173 242 74
Fragments arranged by size:
1411 557 345 242 173 74
NaeI GCC'GGC
Cuts at: 0 867 2616 2802
Size: 867 1749 186
Fragments arranged by size:
1749 867 186
NarI GG'CG_CC
Cuts at: 0 983 2802
Size: 983 1819
NcoI C'CATG_G
Cuts at: 0 2621 2802
Size: 2621 181
NgoMIV G'CCGG_C
Cuts at: 0 865 2614 2802
Size: 865 1749 188
Fragments arranged by size:
1749 865 188
NheI G'CTAG_C
Cuts at: 0 214 2802
Size: 214 2588
NruI TCG'CGA
Cuts at: 0 154 2802
Size: 154 2648
PasI CC'CwG_GG
Cuts at: 0 2601 2802
Size: 2601 201
PflMI CCAn_nnn'nTGG
Cuts at: 0 2758 2802
Size: 2758 44
PfoI T'CCnGG_A
Cuts at: 0 110 374 758 2421 2802
Size: 110 264 384 1663 381
Fragments arranged by size:
1663 384 381 264 110
PmlI CAC'GTG
Cuts at: 0 2667 2802
Size: 2667 135
PpuMI rG'GwC_Cy
Cuts at: 0 659 1446 1698 2802
Size: 659 787 252 1104
Fragments arranged by size:
1104 787 659 252
PspOMI G'GGCC_C
Cuts at: 0 2215 2802
Size: 2215 587
PspXI vC'TCGA_Gb
Cuts at: 0 1742 2802
Size: 1742 1060
PstI C_TGCA'G
Cuts at: 0 2663 2802
Size: 2663 139
PvuII CAG'CTG
Cuts at: 0 1756 2728 2802
Size: 1756 972 74
Fragments arranged by size:
1756 972 74
SacI G_AGCT'C
Cuts at: 0 1726 2197 2596 2802
Size: 1726 471 399 206
Fragments arranged by size:
1726 471 399 206
SanDI GG'GwC_CC
Cuts at: 0 659 1446 2802
Size: 659 787 1356
Fragments arranged by size:
1356 787 659
ScaI AGT'ACT
Cuts at: 0 1887 2802
Size: 1887 915
SexAI A'CCwGG_T
Cuts at: 0 2325 2802
Size: 2325 477
SfcI C'TryA_G
Cuts at: 0 1821 2388 2659 2802
Size: 1821 567 271 143
Fragments arranged by size:
1821 567 271 143
SfoI GGC'GCC
Cuts at: 0 984 2802
Size: 984 1818
SgrAI Cr'CCGG_yG
Cuts at: 0 865 2802
Size: 865 1937
SmaI CCC'GGG
Cuts at: 0 1416 2050 2802
Size: 1416 634 752
Fragments arranged by size:
1416 752 634
SmlI C'TyrA_G
Cuts at: 0 466 1207 1742 2802
Size: 466 741 535 1060
Fragments arranged by size:
1060 741 535 466
StuI AGG'CCT
Cuts at: 0 13 2249 2802
Size: 13 2236 553
Fragments arranged by size:
2236 553 13
StyI C'CwwG_G
Cuts at: 0 159 2621 2802
Size: 159 2462 181
Fragments arranged by size:
2462 181 159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 623 1932 2392 2802
Size: 623 1309 460 410
Fragments arranged by size:
1309 623 460 410
TatI w'GTAC_w
Cuts at: 0 1070 1885 2802
Size: 1070 815 917
Fragments arranged by size:
1070 917 815
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1166 1198 2802
Size: 1166 32 1604
Fragments arranged by size:
1604 1166 32
XbaI T'CTAG_A
Cuts at: 0 2167 2802
Size: 2167 635
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1239 2802
Size: 1239 1563
XhoI C'TCGA_G
Cuts at: 0 466 1742 2802
Size: 466 1276 1060
Fragments arranged by size:
1276 1060 466
XmaI C'CCGG_G
Cuts at: 0 1414 2048 2802
Size: 1414 634 754
Fragments arranged by size:
1414 754 634
XmnI GAAnn'nnTTC
Cuts at: 0 130 1216 1302 2802
Size: 130 1086 86 1500
Fragments arranged by size:
1500 1086 130 86
Enzymes that do cut:
AarI AccI Acc65I AcuI AleI AloI ApaI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BclI BlpI Bme1580I BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BstAPI BstEII BstXI
BstYI BtgI BtgZI BtsI DraIII EaeI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KasI KpnI
MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV
NheI NruI PasI PflMI PfoI PmlI PpuMI PspOMI
PspXI PstI PvuII SacI SanDI ScaI SexAI SfcI
SfoI SgrAI SmaI SmlI StuI StyI TaqII TatI
TstI XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AlfI
AlwNI ApaLI AscI AseI AsiSI AvrII BaeI BciVI
BfrBI BglI BglII BmgBI BsiWI BspEI BspHI BssSI
BstBI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI
EarI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI MfeI MluI NdeI NotI NsiI NspI PacI
PciI PmeI PpiI PshAI PsiI PsrI PvuI RsrII
SacII SalI SapI SbfI SfiI SgfI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I ZraI