Restriction map for
pFN21AE6710
(Linear) (Six-base) MAPSORT of: KIEE6710.seq Check: 971 from: 1 to: 2076
KIEE6710.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1040 1063 1807 2076
Size: 1040 23 744 269
Fragments arranged by size:
1040 744 269 23
AatII G_ACGT'C
Cuts at: 0 446 2076
Size: 446 1630
AccI GT'mk_AC
Cuts at: 0 1862 2076
Size: 1862 214
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2036 2076
Size: 2036 40
AfeI AGC'GCT
Cuts at: 0 225 2076
Size: 225 1851
AleI CACnn'nnGTG
Cuts at: 0 1450 2076
Size: 1450 626
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 193 225 2076
Size: 193 32 1851
Fragments arranged by size:
1851 193 32
AlwNI CAG_nnn'CTG
Cuts at: 0 588 864 1002 1381 1941 2076
Size: 588 276 138 379 560 135
Fragments arranged by size:
588 560 379 276 138 135
ApaI G_GGCC'C
Cuts at: 0 482 549 2076
Size: 482 67 1527
Fragments arranged by size:
1527 482 67
AvaI C'yCGr_G
Cuts at: 0 376 462 2076
Size: 376 86 1614
Fragments arranged by size:
1614 376 86
BanI G'GyrC_C
Cuts at: 0 169 308 427 814 959 1592 1648 1834
Size: 169 139 119 387 145 633 56 186
Cuts at: 1834 2076
Size: 242
Fragments arranged by size:
633 387 242 186 169 145 139 119
56
BanII G_rGCy'C
Cuts at: 0 482 549 700 2076
Size: 482 67 151 1376
Fragments arranged by size:
1376 482 151 67
BbeI G_GCGC'C
Cuts at: 0 173 431 1838 2076
Size: 173 258 1407 238
Fragments arranged by size:
1407 258 238 173
BbsI GAAGACnn'nnnn_
Cuts at: 0 723 2063 2076
Size: 723 1340 13
Fragments arranged by size:
1340 723 13
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1626 1660 1872 1906 2076
Size: 1626 34 212 34 170
Fragments arranged by size:
1626 212 170 34 34
BglI GCCn_nnn'nGGC
Cuts at: 0 44 326 1255 2076
Size: 44 282 929 821
Fragments arranged by size:
929 821 282 44
BglII A'GATC_T
Cuts at: 0 119 2076
Size: 119 1957
BlpI GC'TnA_GC
Cuts at: 0 1237 1386 1987 2076
Size: 1237 149 601 89
Fragments arranged by size:
1237 601 149 89
Bme1580I G_kGCm'C
Cuts at: 0 163 303 311 482 549 2076
Size: 163 140 8 171 67 1527
Fragments arranged by size:
1527 171 163 140 67 8
BmgBI CAC'GTC
Cuts at: 0 231 2076
Size: 231 1845
BmrI ACTGGGnnnn_n'
Cuts at: 0 1577 2076
Size: 1577 499
BmtI G_CTAG'C
Cuts at: 0 248 2076
Size: 248 1828
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 988 1147 1433 1830 2076
Size: 220 768 159 286 397 246
Fragments arranged by size:
768 397 286 246 220 159
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1690 2076
Size: 1690 386
BsaI GGTCTCn'nnnn_
Cuts at: 0 924 2011 2076
Size: 924 1087 65
Fragments arranged by size:
1087 924 65
BsaAI yAC'GTr
Cuts at: 0 615 720 1931 2076
Size: 615 105 1211 145
Fragments arranged by size:
1211 615 145 105
BsaBI GATnn'nnATC
Cuts at: 0 1630 2076
Size: 1630 446
BsaHI Gr'CG_yC
Cuts at: 0 170 428 443 593 1835 2076
Size: 170 258 15 150 1242 241
Fragments arranged by size:
1242 258 241 170 150 15
BsaWI w'CCGG_w
Cuts at: 0 506 762 2076
Size: 506 256 1314
Fragments arranged by size:
1314 506 256
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 193 223 1120 1150 2076
Size: 193 30 897 30 926
Fragments arranged by size:
926 897 193 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 621 1726 2076
Size: 621 1105 350
Fragments arranged by size:
1105 621 350
BseYI C'CCAG_C
Cuts at: 0 351 422 940 1345 2076
Size: 351 71 518 405 731
Fragments arranged by size:
731 518 405 351 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 601 2076
Size: 601 1475
BsiEI CG_ry'CG
Cuts at: 0 39 316 2076
Size: 39 277 1760
Fragments arranged by size:
1760 277 39
BsiHKAI G_wGCw'C
Cuts at: 0 557 1816 1993 2076
Size: 557 1259 177 83
Fragments arranged by size:
1259 557 177 83
BsmI GAATG_Cn'
Cuts at: 0 1432 2076
Size: 1432 644
BsmBI CGTCTCn'nnnn_
Cuts at: 0 600 1311 2076
Size: 600 711 765
Fragments arranged by size:
765 711 600
Bsp1286I G_dGCh'C
Cuts at: 0 163 303 311 482 549 557 700 1816
Size: 163 140 8 171 67 8 143 1116
Cuts at: 1816 1993 2076
Size: 177 83
Fragments arranged by size:
1116 177 171 163 143 140 83 67
8 8
BspEI T'CCGG_A
Cuts at: 0 762 2076
Size: 762 1314
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 1040 1063 1807 2038 2076
Size: 224 816 23 744 231 38
Fragments arranged by size:
816 744 231 224 38 23
BsrBI CCG'CTC
Cuts at: 0 44 2076
Size: 44 2032
BsrFI r'CCGG_y
Cuts at: 0 311 2076
Size: 311 1765
BsrGI T'GTAC_A
Cuts at: 0 1919 2076
Size: 1919 157
BssHII G'CGCG_C
Cuts at: 0 356 2076
Size: 356 1720
BssSI C'ACGA_G
Cuts at: 0 1975 2076
Size: 1975 101
BstAPI GCAn_nnn'nTGC
Cuts at: 0 906 2076
Size: 906 1170
BstEII G'GTnAC_C
Cuts at: 0 1020 2076
Size: 1020 1056
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1171 1266 2076
Size: 1171 95 810
Fragments arranged by size:
1171 810 95
BstYI r'GATC_y
Cuts at: 0 119 416 2076
Size: 119 297 1660
Fragments arranged by size:
1660 297 119
Bsu36I CC'TnA_GG
Cuts at: 0 955 1942 2076
Size: 955 987 134
Fragments arranged by size:
987 955 134
BtgI C'CryG_G
Cuts at: 0 887 1562 1652 2076
Size: 887 675 90 424
Fragments arranged by size:
887 675 424 90
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1869 1875 2076
Size: 1869 6 201
Fragments arranged by size:
1869 201 6
BtsI GCAGTG_nn'
Cuts at: 0 987 1094 1292 1942 2076
Size: 987 107 198 650 134
Fragments arranged by size:
987 650 198 134 107
DraIII CAC_nnn'GTG
Cuts at: 0 1931 2076
Size: 1931 145
DrdI GACnn_nn'nnGTC
Cuts at: 0 1858 2076
Size: 1858 218
EaeI y'GGCC_r
Cuts at: 0 36 313 1067 1268 2076
Size: 36 277 754 201 808
Fragments arranged by size:
808 754 277 201 36
EagI C'GGCC_G
Cuts at: 0 36 313 2076
Size: 36 277 1763
Fragments arranged by size:
1763 277 36
EarI CTCTTCn'nnn_
Cuts at: 0 286 829 837 1158 1321 2076
Size: 286 543 8 321 163 755
Fragments arranged by size:
755 543 321 286 163 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 988 1147 1433 1830 2036 2076
Size: 220 768 159 286 397 206 40
Fragments arranged by size:
768 397 286 220 206 159 40
EcoO109I rG'GnC_Cy
Cuts at: 0 478 545 951 2076
Size: 478 67 406 1125
Fragments arranged by size:
1125 478 406 67
FspI TGC'GCA
Cuts at: 0 450 2076
Size: 450 1626
HaeII r_GCGC'y
Cuts at: 0 173 227 274 352 373 431 1838 2076
Size: 173 54 47 78 21 58 1407 238
Fragments arranged by size:
1407 238 173 78 58 54 47 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1387 1419 2076
Size: 1387 32 657
Fragments arranged by size:
1387 657 32
HincII GTy'rAC
Cuts at: 0 1281 2076
Size: 1281 795
KasI G'GCGC_C
Cuts at: 0 169 427 1834 2076
Size: 169 258 1407 242
Fragments arranged by size:
1407 258 242 169
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 134 2076
Size: 134 1942
MscI TGG'CCA
Cuts at: 0 1270 2076
Size: 1270 806
MslI CAynn'nnrTG
Cuts at: 0 343 1264 1450 2076
Size: 343 921 186 626
Fragments arranged by size:
921 626 343 186
MspA1I CmG'CkG
Cuts at: 0 422 776 1002 1167 2076
Size: 422 354 226 165 909
Fragments arranged by size:
909 422 354 226 165
NarI GG'CG_CC
Cuts at: 0 170 428 1835 2076
Size: 170 258 1407 241
Fragments arranged by size:
1407 258 241 170
NcoI C'CATG_G
Cuts at: 0 887 1652 2076
Size: 887 765 424
Fragments arranged by size:
887 765 424
NheI G'CTAG_C
Cuts at: 0 244 2076
Size: 244 1832
PasI CC'CwG_GG
Cuts at: 0 588 1251 2076
Size: 588 663 825
Fragments arranged by size:
825 663 588
PflMI CCAn_nnn'nTGG
Cuts at: 0 1456 1931 2076
Size: 1456 475 145
Fragments arranged by size:
1456 475 145
PfoI T'CCnGG_A
Cuts at: 0 1569 2076
Size: 1569 507
PmlI CAC'GTG
Cuts at: 0 615 1931 2076
Size: 615 1316 145
Fragments arranged by size:
1316 615 145
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 193 225 2076
Size: 193 32 1851
Fragments arranged by size:
1851 193 32
PpuMI rG'GwC_Cy
Cuts at: 0 951 2076
Size: 951 1125
PspOMI G'GGCC_C
Cuts at: 0 478 545 2076
Size: 478 67 1531
Fragments arranged by size:
1531 478 67
PstI C_TGCA'G
Cuts at: 0 56 221 1639 2076
Size: 56 165 1418 437
Fragments arranged by size:
1418 437 165 56
PvuII CAG'CTG
Cuts at: 0 1002 1167 2076
Size: 1002 165 909
Fragments arranged by size:
1002 909 165
SexAI A'CCwGG_T
Cuts at: 0 176 1283 2076
Size: 176 1107 793
Fragments arranged by size:
1107 793 176
SfcI C'TryA_G
Cuts at: 0 52 217 1635 2076
Size: 52 165 1418 441
Fragments arranged by size:
1418 441 165 52
SfoI GGC'GCC
Cuts at: 0 171 429 1836 2076
Size: 171 258 1407 240
Fragments arranged by size:
1407 258 240 171
SmlI C'TyrA_G
Cuts at: 0 1705 2076
Size: 1705 371
StuI AGG'CCT
Cuts at: 0 1603 2076
Size: 1603 473
StyI C'CwwG_G
Cuts at: 0 887 1652 2076
Size: 887 765 424
Fragments arranged by size:
887 765 424
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 223 1580 2035 2076
Size: 223 1357 455 41
Fragments arranged by size:
1357 455 223 41
TatI w'GTAC_w
Cuts at: 0 395 527 1919 2076
Size: 395 132 1392 157
Fragments arranged by size:
1392 395 157 132
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 717 2076
Size: 717 1359
ZraI GAC'GTC
Cuts at: 0 444 2076
Size: 444 1632
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AleI AloI AlwNI
ApaI AvaI BanI BanII BbeI BbsI BcgI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI
EagI EarI Eco57MI EcoO109I FspI HaeII Hin4I HincII
KasI MmeI MscI MslI MspA1I NarI NcoI NheI
PasI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI
PvuII SexAI SfcI SfoI SmlI StuI StyI TaqII
TatI XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI AhdI AlfI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI
BciVI BclI BfrBI BplI Bpu10I BsiWI BspHI BsrDI
BstBI BstZ17I ClaI CspCI DraI EciI EcoICRI EcoNI
EcoRI EcoRV FalI FseI FspAI HindIII HpaI KpnI
MfeI MluI NaeI NdeI NgoMIV NotI NruI NsiI
NspI PacI PciI PmeI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI
XmnI