Restriction map for
pFN21AE6709
(Linear) (Six-base) MAPSORT of: KIEE6709.seq Check: 7845 from: 1 to: 2139
KIEE6709.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1298 1708 2035 2139
Size: 1298 410 327 104
Fragments arranged by size:
1298 410 327 104
AfeI AGC'GCT
Cuts at: 0 1984 2139
Size: 1984 155
AflIII A'CryG_T
Cuts at: 0 1533 2139
Size: 1533 606
AleI CACnn'nnGTG
Cuts at: 0 463 2139
Size: 463 1676
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2014 2139
Size: 2014 125
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 657 689 2139
Size: 657 32 1450
Fragments arranged by size:
1450 657 32
AlwNI CAG_nnn'CTG
Cuts at: 0 946 1656 2139
Size: 946 710 483
Fragments arranged by size:
946 710 483
ApaI G_GGCC'C
Cuts at: 0 99 305 2139
Size: 99 206 1834
Fragments arranged by size:
1834 206 99
ApoI r'AATT_y
Cuts at: 0 806 1047 1779 1816 2139
Size: 806 241 732 37 323
Fragments arranged by size:
806 732 323 241 37
BamHI G'GATC_C
Cuts at: 0 89 1956 2139
Size: 89 1867 183
Fragments arranged by size:
1867 183 89
BanI G'GyrC_C
Cuts at: 0 130 436 1550 2139
Size: 130 306 1114 589
Fragments arranged by size:
1114 589 306 130
BanII G_rGCy'C
Cuts at: 0 99 305 332 915 1121 2027 2139
Size: 99 206 27 583 206 906 112
Fragments arranged by size:
906 583 206 206 112 99 27
BbeI G_GCGC'C
Cuts at: 0 134 2139
Size: 134 2005
BbvCI CC'TCA_GC
Cuts at: 0 1568 2139
Size: 1568 571
BfrBI ATG'CAT
Cuts at: 0 1157 1503 2139
Size: 1157 346 636
Fragments arranged by size:
1157 636 346
BglI GCCn_nnn'nGGC
Cuts at: 0 65 361 2139
Size: 65 296 1778
Fragments arranged by size:
1778 296 65
BglII A'GATC_T
Cuts at: 0 867 2139
Size: 867 1272
BlpI GC'TnA_GC
Cuts at: 0 856 2139
Size: 856 1283
Bme1580I G_kGCm'C
Cuts at: 0 99 219 305 1555 2088 2139
Size: 99 120 86 1250 533 51
Fragments arranged by size:
1250 533 120 99 86 51
BmgBI CAC'GTC
Cuts at: 0 1536 2139
Size: 1536 603
BmrI ACTGGGnnnn_n'
Cuts at: 0 1390 1550 2139
Size: 1390 160 589
Fragments arranged by size:
1390 589 160
Bpu10I CC'TnA_GC
Cuts at: 0 206 389 1568 2139
Size: 206 183 1179 571
Fragments arranged by size:
1179 571 206 183
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 273 1416 1783 2139
Size: 273 1143 367 356
Fragments arranged by size:
1143 367 356 273
BsaI GGTCTCn'nnnn_
Cuts at: 0 125 763 2139
Size: 125 638 1376
Fragments arranged by size:
1376 638 125
BsaBI GATnn'nnATC
Cuts at: 0 473 2139
Size: 473 1666
BsaHI Gr'CG_yC
Cuts at: 0 131 2139
Size: 131 2008
BseYI C'CCAG_C
Cuts at: 0 18 733 1120 2139
Size: 18 715 387 1019
Fragments arranged by size:
1019 715 387 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1118 2139
Size: 1118 1021
BsiEI CG_ry'CG
Cuts at: 0 1922 2139
Size: 1922 217
BsiHKAI G_wGCw'C
Cuts at: 0 332 2139
Size: 332 1807
BsmI GAATG_Cn'
Cuts at: 0 397 1503 2139
Size: 397 1106 636
Fragments arranged by size:
1106 636 397
Bsp1286I G_dGCh'C
Cuts at: 0 99 219 305 332 915 1121 1555 2027
Size: 99 120 86 27 583 206 434 472
Cuts at: 2027 2088 2139
Size: 61 51
Fragments arranged by size:
583 472 434 206 120 99 86 61
51 27
BspHI T'CATG_A
Cuts at: 0 1269 2139
Size: 1269 870
BsrBI CCG'CTC
Cuts at: 0 1918 2139
Size: 1918 221
BssSI C'ACGA_G
Cuts at: 0 1873 2139
Size: 1873 266
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1509 2139
Size: 1509 630
BstBI TT'CG_AA
Cuts at: 0 2114 2139
Size: 2114 25
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2034 2139
Size: 2034 105
BstYI r'GATC_y
Cuts at: 0 89 867 1956 2103 2139
Size: 89 778 1089 147 36
Fragments arranged by size:
1089 778 147 89 36
Bsu36I CC'TnA_GG
Cuts at: 0 1774 2139
Size: 1774 365
BtgI C'CryG_G
Cuts at: 0 125 440 1356 1758 1928 2007 2139
Size: 125 315 916 402 170 79 132
Fragments arranged by size:
916 402 315 170 132 125 79
BtsI GCAGTG_nn'
Cuts at: 0 620 1654 2139
Size: 620 1034 485
Fragments arranged by size:
1034 620 485
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 524 559 866 901 2139
Size: 524 35 307 35 1238
Fragments arranged by size:
1238 524 307 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 1875 2139
Size: 1875 264
EaeI y'GGCC_r
Cuts at: 0 353 2139
Size: 353 1786
EcoICRI GAG'CTC
Cuts at: 0 330 2139
Size: 330 1809
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1298 1708 2035 2139
Size: 1298 410 327 104
Fragments arranged by size:
1298 410 327 104
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1223 2139
Size: 1223 916
EcoO109I rG'GnC_Cy
Cuts at: 0 302 1068 2139
Size: 302 766 1071
Fragments arranged by size:
1071 766 302
EcoRI G'AATT_C
Cuts at: 0 1779 2139
Size: 1779 360
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 714 2065 2139
Size: 714 1351 74
Fragments arranged by size:
1351 714 74
HaeII r_GCGC'y
Cuts at: 0 134 186 1986 2139
Size: 134 52 1800 153
Fragments arranged by size:
1800 153 134 52
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 1458 1490 2139
Size: 316 32 1110 32 649
Fragments arranged by size:
1110 649 316 32 32
HincII GTy'rAC
Cuts at: 0 1620 2139
Size: 1620 519
HindIII A'AGCT_T
Cuts at: 0 419 2139
Size: 419 1720
HpaI GTT'AAC
Cuts at: 0 1620 2139
Size: 1620 519
KasI G'GCGC_C
Cuts at: 0 130 2139
Size: 130 2009
MfeI C'AATT_G
Cuts at: 0 832 2139
Size: 832 1307
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1468 2120 2139
Size: 1468 652 19
Fragments arranged by size:
1468 652 19
MslI CAynn'nnrTG
Cuts at: 0 463 2032 2139
Size: 463 1569 107
Fragments arranged by size:
1569 463 107
MspA1I CmG'CkG
Cuts at: 0 116 1711 1857 2139
Size: 116 1595 146 282
Fragments arranged by size:
1595 282 146 116
NarI GG'CG_CC
Cuts at: 0 131 2139
Size: 131 2008
NcoI C'CATG_G
Cuts at: 0 440 1758 1928 2139
Size: 440 1318 170 211
Fragments arranged by size:
1318 440 211 170
NdeI CA'TA_TG
Cuts at: 0 1833 2139
Size: 1833 306
NsiI A_TGCA'T
Cuts at: 0 1159 1505 2139
Size: 1159 346 634
Fragments arranged by size:
1159 634 346
NspI r_CATG'y
Cuts at: 0 287 1157 1498 2139
Size: 287 870 341 641
Fragments arranged by size:
870 641 341 287
PasI CC'CwG_GG
Cuts at: 0 153 1064 2139
Size: 153 911 1075
Fragments arranged by size:
1075 911 153
PflMI CCAn_nnn'nTGG
Cuts at: 0 1488 1875 1934 2139
Size: 1488 387 59 205
Fragments arranged by size:
1488 387 205 59
PpuMI rG'GwC_Cy
Cuts at: 0 1068 2139
Size: 1068 1071
PsiI TTA'TAA
Cuts at: 0 803 2139
Size: 803 1336
PspOMI G'GGCC_C
Cuts at: 0 95 301 2139
Size: 95 206 1838
Fragments arranged by size:
1838 206 95
PstI C_TGCA'G
Cuts at: 0 1710 2139
Size: 1710 429
PvuII CAG'CTG
Cuts at: 0 116 1711 1857 2139
Size: 116 1595 146 282
Fragments arranged by size:
1595 282 146 116
SacI G_AGCT'C
Cuts at: 0 332 2139
Size: 332 1807
SanDI GG'GwC_CC
Cuts at: 0 1068 2139
Size: 1068 1071
ScaI AGT'ACT
Cuts at: 0 1594 2139
Size: 1594 545
SexAI A'CCwGG_T
Cuts at: 0 1546 2139
Size: 1546 593
SfcI C'TryA_G
Cuts at: 0 1080 1706 1951 2139
Size: 1080 626 245 188
Fragments arranged by size:
1080 626 245 188
SfoI GGC'GCC
Cuts at: 0 132 2139
Size: 132 2007
SmlI C'TyrA_G
Cuts at: 0 252 1431 1798 2139
Size: 252 1179 367 341
Fragments arranged by size:
1179 367 341 252
SphI G_CATG'C
Cuts at: 0 1157 2139
Size: 1157 982
SspI AAT'ATT
Cuts at: 0 1113 1173 2139
Size: 1113 60 966
Fragments arranged by size:
1113 966 60
StuI AGG'CCT
Cuts at: 0 1166 2139
Size: 1166 973
StyI C'CwwG_G
Cuts at: 0 440 918 1758 1928 2139
Size: 440 478 840 170 211
Fragments arranged by size:
840 478 440 211 170
TatI w'GTAC_w
Cuts at: 0 649 1592 2139
Size: 649 943 547
Fragments arranged by size:
943 649 547
XbaI T'CTAG_A
Cuts at: 0 588 2139
Size: 588 1551
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1537 1847 2139
Size: 1537 310 292
Fragments arranged by size:
1537 310 292
XmnI GAAnn'nnTTC
Cuts at: 0 227 710 2139
Size: 227 483 1429
Fragments arranged by size:
1429 483 227
Enzymes that do cut:
AcuI AfeI AflIII AleI AlfI AloI AlwNI ApaI
ApoI BamHI BanI BanII BbeI BbvCI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI Bpu10I BpuEI BsaI
BsaBI BsaHI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BsrBI BssSI BstAPI BstBI BstXI BstYI Bsu36I
BtgI BtsI CspCI DraIII EaeI EcoICRI Eco57MI EcoNI
EcoO109I EcoRI FalI HaeII Hin4I HincII HindIII HpaI
KasI MfeI MmeI MslI MspA1I NarI NcoI NdeI
NsiI NspI PasI PflMI PpuMI PsiI PspOMI PstI
PvuII SacI SanDI ScaI SexAI SfcI SfoI SmlI
SphI SspI StuI StyI TatI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbsI
BcgI BciVI BclI BmtI BplI BpmI BsaAI BsaWI
BsaXI BseRI BsiWI BsmBI BspEI BspMI BsrDI BsrFI
BsrGI BssHII BstEII BstZ17I BtgZI ClaI DraI DrdI
EagI EarI EciI EcoRV FseI FspI FspAI KpnI
MluI MscI NaeI NgoMIV NheI NotI NruI PacI
PciI PfoI PmeI PmlI PpiI PshAI PspXI PsrI
PvuI RsrII SacII SalI SapI SbfI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SwaI TaqII TstI
Tth111I XhoI XmaI ZraI