Restriction map for
pFN21AE6707
(Linear) (Six-base) MAPSORT of: KIEE6707.seq Check: 4017 from: 1 to: 3729
KIEE6707.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 307 3729
Size: 307 3422
AatII G_ACGT'C
Cuts at: 0 2834 3729
Size: 2834 895
AccI GT'mk_AC
Cuts at: 0 2864 2981 3389 3729
Size: 2864 117 408 340
Fragments arranged by size:
2864 408 340 117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1927 3054 3729
Size: 1927 1127 675
Fragments arranged by size:
1927 1127 675
AfeI AGC'GCT
Cuts at: 0 2488 3729
Size: 2488 1241
AhdI GACnn_n'nnGTC
Cuts at: 0 1515 3729
Size: 1515 2214
AleI CACnn'nnGTG
Cuts at: 0 2773 3729
Size: 2773 956
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1028 3542 3729
Size: 1028 2514 187
Fragments arranged by size:
2514 1028 187
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2731 2763 3729
Size: 2731 32 966
Fragments arranged by size:
2731 966 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1174 1327 1365 2588 2883 3158 3327 3471
Size: 1174 153 38 1223 295 275 169 144
Cuts at: 3471 3729
Size: 258
Fragments arranged by size:
1223 1174 295 275 258 169 153 144
38
ApaI G_GGCC'C
Cuts at: 0 155 554 666 1212 2825 3634 3658 3729
Size: 155 399 112 546 1613 809 24 71
Fragments arranged by size:
1613 809 546 399 155 112 71 24
ApoI r'AATT_y
Cuts at: 0 316 3729
Size: 316 3413
AvaI C'yCGr_G
Cuts at: 0 154 811 1016 1921 2215 2530 2928 3411
Size: 154 657 205 905 294 315 398 483
Cuts at: 3411 3534 3543 3624 3657 3729
Size: 123 9 81 33 72
Fragments arranged by size:
905 657 483 398 315 294 205 154
123 81 72 33 9
BamHI G'GATC_C
Cuts at: 0 2897 3729
Size: 2897 832
BanI G'GyrC_C
Cuts at: 0 856 1437 1588 1654 1744 2025 2367 2395
Size: 856 581 151 66 90 281 342 28
Cuts at: 2395 2908 3136 3729
Size: 513 228 593
Fragments arranged by size:
856 593 581 513 342 281 228 151
90 66 28
BanII G_rGCy'C
Cuts at: 0 155 199 554 666 1212 1372 1492 1922
Size: 155 44 355 112 546 160 120 430
Cuts at: 1922 1930 1979 2222 2825 3517 3634 3658 3729
Size: 8 49 243 603 692 117 24 71
Fragments arranged by size:
692 603 546 430 355 243 160 155
120 117 112 71 49 44 24 8
BbeI G_GCGC'C
Cuts at: 0 1592 3140 3729
Size: 1592 1548 589
Fragments arranged by size:
1592 1548 589
BbsI GAAGACnn'nnnn_
Cuts at: 0 366 1080 3060 3729
Size: 366 714 1980 669
Fragments arranged by size:
1980 714 669 366
BbvCI CC'TCA_GC
Cuts at: 0 1262 3729
Size: 1262 2467
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3218 3252 3729
Size: 3218 34 477
Fragments arranged by size:
3218 477 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1381 2143 3729
Size: 1381 762 1586
Fragments arranged by size:
1586 1381 762
BglII A'GATC_T
Cuts at: 0 2171 3729
Size: 2171 1558
BlpI GC'TnA_GC
Cuts at: 0 706 1366 2800 3729
Size: 706 660 1434 929
Fragments arranged by size:
1434 929 706 660
Bme1580I G_kGCm'C
Cuts at: 0 155 225 531 554 666 729 1212 1442
Size: 155 70 306 23 112 63 483 230
Cuts at: 1442 1747 2267 2372 2825 3634 3658 3729
Size: 305 520 105 453 809 24 71
Fragments arranged by size:
809 520 483 453 306 305 230 155
112 105 71 70 63 24 23
BmgBI CAC'GTC
Cuts at: 0 2604 3147 3729
Size: 2604 543 582
Fragments arranged by size:
2604 582 543
BmrI ACTGGGnnnn_n'
Cuts at: 0 534 606 1141 1807 2107 2598 2941 3729
Size: 534 72 535 666 300 491 343 788
Fragments arranged by size:
788 666 535 534 491 343 300 72
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 336 1050 1812 3729
Size: 336 714 762 1917
Fragments arranged by size:
1917 762 714 336
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1196 1349 2161 2319 2368 2377 2503 2867
Size: 1196 153 812 158 49 9 126 364
Cuts at: 2867 3729
Size: 862
Fragments arranged by size:
1196 862 812 364 158 153 126 49
9
Bpu10I CC'TnA_GC
Cuts at: 0 382 1262 1486 1916 3729
Size: 382 880 224 430 1813
Fragments arranged by size:
1813 880 430 382 224
BsaI GGTCTCn'nnnn_
Cuts at: 0 328 2073 3374 3729
Size: 328 1745 1301 355
Fragments arranged by size:
1745 1301 355 328
BsaAI yAC'GTr
Cuts at: 0 192 1422 3729
Size: 192 1230 2307
Fragments arranged by size:
2307 1230 192
BsaHI Gr'CG_yC
Cuts at: 0 1334 1589 2831 3137 3260 3729
Size: 1334 255 1242 306 123 469
Fragments arranged by size:
1334 1242 469 306 255 123
BsaWI w'CCGG_w
Cuts at: 0 1800 2735 3729
Size: 1800 935 994
Fragments arranged by size:
1800 994 935
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 181 211 956 986 1324 1354 1679 1709
Size: 181 30 745 30 338 30 325 30
Cuts at: 1709 2476 2506 2840 2870 3729
Size: 767 30 334 30 859
Fragments arranged by size:
859 767 745 338 334 325 181 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 106 411 751 1096 1956 2365 2680 3522
Size: 106 305 340 345 860 409 315 842
Cuts at: 3522 3729
Size: 207
Fragments arranged by size:
860 842 409 345 340 315 305 207
106
BseYI C'CCAG_C
Cuts at: 0 59 758 803 1254 1886 1897 2038 2157
Size: 59 699 45 451 632 11 141 119
Cuts at: 2157 2438 2452 2967 3399 3634 3729
Size: 281 14 515 432 235 95
Fragments arranged by size:
699 632 515 451 432 281 235 141
119 95 59 45 14 11
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1282 1378 3352 3729
Size: 1282 96 1974 377
Fragments arranged by size:
1974 1282 377 96
BsiEI CG_ry'CG
Cuts at: 0 2195 3202 3729
Size: 2195 1007 527
Fragments arranged by size:
2195 1007 527
BsiHKAI G_wGCw'C
Cuts at: 0 826 1372 3729
Size: 826 546 2357
Fragments arranged by size:
2357 826 546
BsmI GAATG_Cn'
Cuts at: 0 273 2665 3031 3729
Size: 273 2392 366 698
Fragments arranged by size:
2392 698 366 273
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1325 3105 3270 3729
Size: 1325 1780 165 459
Fragments arranged by size:
1780 1325 459 165
Bsp1286I G_dGCh'C
Cuts at: 0 155 199 225 531 554 666 729 826
Size: 155 44 26 306 23 112 63 97
Cuts at: 826 1212 1372 1442 1492 1747 1922 1930 1979
Size: 386 160 70 50 255 175 8 49
Cuts at: 1979 2222 2267 2372 2825 3517 3634 3658 3729
Size: 243 45 105 453 692 117 24 71
Fragments arranged by size:
692 453 386 306 255 243 175 160
155 117 112 105 97 71 70 63
50 49 45 44 26 24 23 8
BspEI T'CCGG_A
Cuts at: 0 1800 2735 3729
Size: 1800 935 994
Fragments arranged by size:
1800 994 935
BspHI T'CATG_A
Cuts at: 0 2870 3729
Size: 2870 859
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 237 307 353 1662 2715 3729
Size: 237 70 46 1309 1053 1014
Fragments arranged by size:
1309 1053 1014 237 70 46
BsrBI CCG'CTC
Cuts at: 0 386 2643 3729
Size: 386 2257 1086
Fragments arranged by size:
2257 1086 386
BsrDI GCAATG_nn'
Cuts at: 0 1010 1604 3317 3729
Size: 1010 594 1713 412
Fragments arranged by size:
1713 1010 594 412
BsrFI r'CCGG_y
Cuts at: 0 134 1657 3197 3358 3729
Size: 134 1523 1540 161 371
Fragments arranged by size:
1540 1523 371 161 134
BssHII G'CGCG_C
Cuts at: 0 2963 3729
Size: 2963 766
BstXI CCAn_nnnn'nTGG
Cuts at: 0 39 1905 2469 3381 3678 3729
Size: 39 1866 564 912 297 51
Fragments arranged by size:
1866 912 564 297 51 39
BstYI r'GATC_y
Cuts at: 0 122 2171 2477 2732 2897 3729
Size: 122 2049 306 255 165 832
Fragments arranged by size:
2049 832 306 255 165 122
BstZ17I GTA'TAC
Cuts at: 0 3390 3729
Size: 3390 339
Bsu36I CC'TnA_GG
Cuts at: 0 872 1406 1649 3729
Size: 872 534 243 2080
Fragments arranged by size:
2080 872 534 243
BtgI C'CryG_G
Cuts at: 0 1592 1770 3048 3140 3255 3587 3729
Size: 1592 178 1278 92 115 332 142
Fragments arranged by size:
1592 1278 332 178 142 115 92
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2232 3729
Size: 2232 1497
BtsI GCAGTG_nn'
Cuts at: 0 223 446 792 928 1555 2045 2418 3466
Size: 223 223 346 136 627 490 373 1048
Cuts at: 3466 3729
Size: 263
Fragments arranged by size:
1048 627 490 373 346 263 223 223
136
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1587 1622 3729
Size: 1587 35 2107
Fragments arranged by size:
2107 1587 35
DraIII CAC_nnn'GTG
Cuts at: 0 183 3222 3729
Size: 183 3039 507
Fragments arranged by size:
3039 507 183
DrdI GACnn_nn'nnGTC
Cuts at: 0 2065 3729
Size: 2065 1664
EaeI y'GGCC_r
Cuts at: 0 128 767 842 1562 1595 1907 2192 2312
Size: 128 639 75 720 33 312 285 120
Cuts at: 2312 2420 2644 3199 3356 3729
Size: 108 224 555 157 373
Fragments arranged by size:
720 639 555 373 312 285 224 157
128 120 108 75 33
EagI C'GGCC_G
Cuts at: 0 2192 3199 3729
Size: 2192 1007 530
Fragments arranged by size:
2192 1007 530
EarI CTCTTCn'nnn_
Cuts at: 0 248 461 728 1037 1291 2089 2188 2578
Size: 248 213 267 309 254 798 99 390
Cuts at: 2578 3729
Size: 1151
Fragments arranged by size:
1151 798 390 309 267 254 248 213
99
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1362 1366 2586 2671 3658 3729
Size: 1362 4 1220 85 987 71
Fragments arranged by size:
1362 1220 987 85 71 4
EcoICRI GAG'CTC
Cuts at: 0 1370 3729
Size: 1370 2359
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1196 1349 1927 2161 2319 2368 2377 2503
Size: 1196 153 578 234 158 49 9 126
Cuts at: 2503 2867 3054 3729
Size: 364 187 675
Fragments arranged by size:
1196 675 578 364 234 187 158 153
126 49 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2623 2694 3331 3729
Size: 2623 71 637 398
Fragments arranged by size:
2623 637 398 71
EcoO109I rG'GnC_Cy
Cuts at: 0 471 550 551 876 1208 1233 1324 1821
Size: 471 79 1 325 332 25 91 497
Cuts at: 1821 2822 3538 3630 3631 3654 3729
Size: 1001 716 92 1 23 75
Fragments arranged by size:
1001 716 497 471 332 325 92 91
79 75 25 23 1 1
FseI GG_CCGG'CC
Cuts at: 0 3201 3729
Size: 3201 528
FspI TGC'GCA
Cuts at: 0 1272 2119 2992 3601 3729
Size: 1272 847 873 609 128
Fragments arranged by size:
1272 873 847 609 128
FspAI rTGC'GCAy
Cuts at: 0 2992 3729
Size: 2992 737
HaeII r_GCGC'y
Cuts at: 0 1429 1592 2490 2539 2811 3140 3400 3729
Size: 1429 163 898 49 272 329 260 329
Fragments arranged by size:
1429 898 329 329 272 260 163 49
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 392 424 820 852 1324 1356 1501 1533
Size: 392 32 396 32 472 32 145 32
Cuts at: 1533 2284 2316 2656 2688 3729
Size: 751 32 340 32 1041
Fragments arranged by size:
1041 751 472 396 392 340 145 32
32 32 32 32 32
KasI G'GCGC_C
Cuts at: 0 1588 3136 3729
Size: 1588 1548 593
Fragments arranged by size:
1588 1548 593
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1505 2774 3729
Size: 1505 1269 955
Fragments arranged by size:
1505 1269 955
MscI TGG'CCA
Cuts at: 0 130 769 1564 1597 1909 2422 3729
Size: 130 639 795 33 312 513 1307
Fragments arranged by size:
1307 795 639 513 312 130 33
MslI CAynn'nnrTG
Cuts at: 0 2773 3017 3524 3729
Size: 2773 244 507 205
Fragments arranged by size:
2773 507 244 205
MspA1I CmG'CkG
Cuts at: 0 113 164 1304 2011 2652 2883 3050 3504
Size: 113 51 1140 707 641 231 167 454
Cuts at: 3504 3615 3729
Size: 111 114
Fragments arranged by size:
1140 707 641 454 231 167 114 113
111 51
NaeI GCC'GGC
Cuts at: 0 1659 3199 3729
Size: 1659 1540 530
Fragments arranged by size:
1659 1540 530
NarI GG'CG_CC
Cuts at: 0 1589 3137 3729
Size: 1589 1548 592
Fragments arranged by size:
1589 1548 592
NgoMIV G'CCGG_C
Cuts at: 0 1657 3197 3729
Size: 1657 1540 532
Fragments arranged by size:
1657 1540 532
NspI r_CATG'y
Cuts at: 0 606 753 1005 1271 3529 3729
Size: 606 147 252 266 2258 200
Fragments arranged by size:
2258 606 266 252 200 147
PasI CC'CwG_GG
Cuts at: 0 1220 2286 2696 3729
Size: 1220 1066 410 1033
Fragments arranged by size:
1220 1066 1033 410
PflMI CCAn_nnn'nTGG
Cuts at: 0 765 1174 2588 3729
Size: 765 409 1414 1141
Fragments arranged by size:
1414 1141 765 409
PfoI T'CCnGG_A
Cuts at: 0 262 410 1313 2480 3729
Size: 262 148 903 1167 1249
Fragments arranged by size:
1249 1167 903 262 148
PmlI CAC'GTG
Cuts at: 0 192 1422 3729
Size: 192 1230 2307
Fragments arranged by size:
2307 1230 192
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1030 1062 3729
Size: 1030 32 2667
Fragments arranged by size:
2667 1030 32
PpuMI rG'GwC_Cy
Cuts at: 0 471 1821 3538 3729
Size: 471 1350 1717 191
Fragments arranged by size:
1717 1350 471 191
PspOMI G'GGCC_C
Cuts at: 0 151 550 662 1208 2821 3630 3654 3729
Size: 151 399 112 546 1613 809 24 75
Fragments arranged by size:
1613 809 546 399 151 112 75 24
PspXI vC'TCGA_Gb
Cuts at: 0 2215 3624 3729
Size: 2215 1409 105
Fragments arranged by size:
2215 1409 105
PstI C_TGCA'G
Cuts at: 0 248 2327 3323 3729
Size: 248 2079 996 406
Fragments arranged by size:
2079 996 406 248
PvuII CAG'CTG
Cuts at: 0 2652 3504 3615 3729
Size: 2652 852 111 114
Fragments arranged by size:
2652 852 114 111
SacI G_AGCT'C
Cuts at: 0 1372 3729
Size: 1372 2357
SacII CC_GC'GG
Cuts at: 0 3051 3729
Size: 3051 678
SanDI GG'GwC_CC
Cuts at: 0 1821 3538 3729
Size: 1821 1717 191
Fragments arranged by size:
1821 1717 191
SbfI CC_TGCA'GG
Cuts at: 0 248 3729
Size: 248 3481
SexAI A'CCwGG_T
Cuts at: 0 50 3729
Size: 50 3679
SfcI C'TryA_G
Cuts at: 0 244 2323 2679 3319 3451 3729
Size: 244 2079 356 640 132 278
Fragments arranged by size:
2079 640 356 278 244 132
SfoI GGC'GCC
Cuts at: 0 1590 3138 3729
Size: 1590 1548 591
Fragments arranged by size:
1590 1548 591
SmaI CCC'GGG
Cuts at: 0 156 813 3659 3729
Size: 156 657 2846 70
Fragments arranged by size:
2846 657 156 70
SmlI C'TyrA_G
Cuts at: 0 2215 3624 3729
Size: 2215 1409 105
Fragments arranged by size:
2215 1409 105
SphI G_CATG'C
Cuts at: 0 606 753 1005 1271 3529 3729
Size: 606 147 252 266 2258 200
Fragments arranged by size:
2258 606 266 252 200 147
StuI AGG'CCT
Cuts at: 0 676 1411 1624 1847 3729
Size: 676 735 213 223 1882
Fragments arranged by size:
1882 735 676 223 213
StyI C'CwwG_G
Cuts at: 0 233 1094 1412 3122 3554 3729
Size: 233 861 318 1710 432 175
Fragments arranged by size:
1710 861 432 318 233 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 480 1233 1464 2394 3301 3684 3729
Size: 480 753 231 930 907 383 45
Fragments arranged by size:
930 907 753 480 383 231 45
TatI w'GTAC_w
Cuts at: 0 455 2522 3729
Size: 455 2067 1207
Fragments arranged by size:
2067 1207 455
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 181 213 956 988 2397 2429 2476 2508
Size: 181 32 743 32 1409 32 47 32
Cuts at: 2508 2532 2564 2840 2872 3729
Size: 24 32 276 32 857
Fragments arranged by size:
1409 857 743 276 181 47 32 32
32 32 32 32 24
Tth111I GACn'n_nGTC
Cuts at: 0 2833 3148 3729
Size: 2833 315 581
Fragments arranged by size:
2833 581 315
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2343 2466 3729
Size: 2343 123 1263
Fragments arranged by size:
2343 1263 123
XhoI C'TCGA_G
Cuts at: 0 2215 3624 3729
Size: 2215 1409 105
Fragments arranged by size:
2215 1409 105
XmaI C'CCGG_G
Cuts at: 0 154 811 3657 3729
Size: 154 657 2846 72
Fragments arranged by size:
2846 657 154 72
ZraI GAC'GTC
Cuts at: 0 2832 3729
Size: 2832 897
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AhdI AleI AlfI
AloI AlwNI ApaI ApoI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BglI BglII BlpI Bme1580I
BmgBI BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FseI FspI FspAI HaeII Hin4I
KasI MmeI MscI MslI MspA1I NaeI NarI NgoMIV
NspI PasI PflMI PfoI PmlI PpiI PpuMI PspOMI
PspXI PstI PvuII SacI SacII SanDI SbfI SexAI
SfcI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XhoI XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AgeI ApaLI AscI AseI
AsiSI AvrII BaeI BciVI BclI BfrBI BmtI BpuEI
BsaBI BsiWI BsrGI BssSI BstAPI BstBI BstEII ClaI
DraI EcoRI EcoRV FalI HincII HindIII HpaI KpnI
MfeI MluI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PshAI PsiI PsrI PvuI RsrII
SalI SapI ScaI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XbaI XmnI