Restriction map for
pFN21AE6706
(Linear) (Six-base) MAPSORT of: KIEE6706.seq Check: 4957 from: 1 to: 3012
KIEE6706.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 30 881 3012
Size: 30 851 2131
Fragments arranged by size:
2131 851 30
AccI GT'mk_AC
Cuts at: 0 826 1097 1511 2336 3012
Size: 826 271 414 825 676
Fragments arranged by size:
826 825 676 414 271
AclI AA'CG_TT
Cuts at: 0 528 3012
Size: 528 2484
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 1261 1379 1775 2213 2368 2941 3012
Size: 345 916 118 396 438 155 573 71
Fragments arranged by size:
916 573 438 396 345 155 118 71
AgeI A'CCGG_T
Cuts at: 0 68 967 3012
Size: 68 899 2045
Fragments arranged by size:
2045 899 68
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 644 676 2156 2188 3012
Size: 644 32 1480 32 824
Fragments arranged by size:
1480 824 644 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 150 873 1357 3012
Size: 150 723 484 1655
Fragments arranged by size:
1655 723 484 150
ApoI r'AATT_y
Cuts at: 0 505 725 993 1458 3012
Size: 505 220 268 465 1554
Fragments arranged by size:
1554 505 465 268 220
AseI AT'TA_AT
Cuts at: 0 497 1816 1829 1961 3012
Size: 497 1319 13 132 1051
Fragments arranged by size:
1319 1051 497 132 13
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1403 1436 3012
Size: 1403 33 1576
Fragments arranged by size:
1576 1403 33
BanI G'GyrC_C
Cuts at: 0 45 1667 3012
Size: 45 1622 1345
Fragments arranged by size:
1622 1345 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 1383 2660 3012
Size: 1383 1277 352
Fragments arranged by size:
1383 1277 352
BbvCI CC'TCA_GC
Cuts at: 0 1445 2324 3012
Size: 1445 879 688
Fragments arranged by size:
1445 879 688
BciVI GTATCCnnnnn_n'
Cuts at: 0 325 1115 1992 3012
Size: 325 790 877 1020
Fragments arranged by size:
1020 877 790 325
BglII A'GATC_T
Cuts at: 0 2064 2571 3012
Size: 2064 507 441
Fragments arranged by size:
2064 507 441
BlpI GC'TnA_GC
Cuts at: 0 1910 2351 3012
Size: 1910 441 661
Fragments arranged by size:
1910 661 441
Bme1580I G_kGCm'C
Cuts at: 0 50 1670 1928 3012
Size: 50 1620 258 1084
Fragments arranged by size:
1620 1084 258 50
BmgBI CAC'GTC
Cuts at: 0 1152 2526 3012
Size: 1152 1374 486
Fragments arranged by size:
1374 1152 486
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 460 525 2459 3012
Size: 460 65 1934 553
Fragments arranged by size:
1934 553 460 65
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 236 300 652 841 928 1643 2442
Size: 79 157 64 352 189 87 715 799
Cuts at: 2442 3012
Size: 570
Fragments arranged by size:
799 715 570 352 189 157 87 79
64
Bpu10I CC'TnA_GC
Cuts at: 0 1445 2303 2324 3012
Size: 1445 858 21 688
Fragments arranged by size:
1445 858 688 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 757 3012
Size: 757 2255
BsaI GGTCTCn'nnnn_
Cuts at: 0 359 431 3012
Size: 359 72 2581
Fragments arranged by size:
2581 359 72
BsaAI yAC'GTr
Cuts at: 0 265 3012
Size: 265 2747
BsaWI w'CCGG_w
Cuts at: 0 68 967 2792 3012
Size: 68 899 1825 220
Fragments arranged by size:
1825 899 220 68
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 644 674 926 956 1207 1237 3012
Size: 644 30 252 30 251 30 1775
Fragments arranged by size:
1775 644 252 251 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1228 3012
Size: 1228 1784
BseYI C'CCAG_C
Cuts at: 0 180 1450 1700 3012
Size: 180 1270 250 1312
Fragments arranged by size:
1312 1270 250 180
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 25 438 1212 3012
Size: 4 21 413 774 1800
Fragments arranged by size:
1800 774 413 21 4
BsmI GAATG_Cn'
Cuts at: 0 1553 1704 3012
Size: 1553 151 1308
Fragments arranged by size:
1553 1308 151
Bsp1286I G_dGCh'C
Cuts at: 0 50 1670 1928 3012
Size: 50 1620 258 1084
Fragments arranged by size:
1620 1084 258 50
BspHI T'CATG_A
Cuts at: 0 2772 2930 3012
Size: 2772 158 82
Fragments arranged by size:
2772 158 82
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 26 30 881 1178 3012
Size: 26 4 851 297 1834
Fragments arranged by size:
1834 851 297 26 4
BsrDI GCAATG_nn'
Cuts at: 0 2458 3012
Size: 2458 554
BsrFI r'CCGG_y
Cuts at: 0 68 139 967 2665 3012
Size: 68 71 828 1698 347
Fragments arranged by size:
1698 828 347 71 68
BsrGI T'GTAC_A
Cuts at: 0 11 374 1496 3012
Size: 11 363 1122 1516
Fragments arranged by size:
1516 1122 363 11
BssSI C'ACGA_G
Cuts at: 0 305 1208 3012
Size: 305 903 1804
Fragments arranged by size:
1804 903 305
BstAPI GCAn_nnn'nTGC
Cuts at: 0 45 3012
Size: 45 2967
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1614 3012
Size: 1614 1398
BstYI r'GATC_y
Cuts at: 0 2064 2475 2571 3012
Size: 2064 411 96 441
Fragments arranged by size:
2064 441 411 96
BtgI C'CryG_G
Cuts at: 0 160 2515 3012
Size: 160 2355 497
Fragments arranged by size:
2355 497 160
BtsI GCAGTG_nn'
Cuts at: 0 1180 1200 2051 3012
Size: 1180 20 851 961
Fragments arranged by size:
1180 961 851 20
ClaI AT'CG_AT
Cuts at: 0 2017 3012
Size: 2017 995
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1127 1162 3012
Size: 1127 35 1850
Fragments arranged by size:
1850 1127 35
DraI TTT'AAA
Cuts at: 0 1323 3012
Size: 1323 1689
DrdI GACnn_nn'nnGTC
Cuts at: 0 613 3012
Size: 613 2399
EaeI y'GGCC_r
Cuts at: 0 137 141 3012
Size: 137 4 2871
Fragments arranged by size:
2871 137 4
EarI CTCTTCn'nnn_
Cuts at: 0 233 2780 3012
Size: 233 2547 232
Fragments arranged by size:
2547 233 232
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 236 300 345 652 841 928 1261
Size: 79 157 64 45 307 189 87 333
Cuts at: 1261 1379 1643 1775 2213 2368 2442 2941 3012
Size: 118 264 132 438 155 74 499 71
Fragments arranged by size:
499 438 333 307 264 189 157 155
132 118 87 79 74 71 64 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 431 3012
Size: 431 2581
EcoO109I rG'GnC_Cy
Cuts at: 0 402 640 1218 3012
Size: 402 238 578 1794
Fragments arranged by size:
1794 578 402 238
EcoRI G'AATT_C
Cuts at: 0 505 3012
Size: 505 2507
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 457 3012
Size: 457 2555
FseI GG_CCGG'CC
Cuts at: 0 143 3012
Size: 143 2869
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 672 704 889 921 957 989 1802 1834
Size: 672 32 185 32 36 32 813 32
Cuts at: 1834 3012
Size: 1178
Fragments arranged by size:
1178 813 672 185 36 32 32 32
32
MfeI C'AATT_G
Cuts at: 0 233 2897 3012
Size: 233 2664 115
Fragments arranged by size:
2664 233 115
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 564 2200 3012
Size: 564 1636 812
Fragments arranged by size:
1636 812 564
MslI CAynn'nnrTG
Cuts at: 0 2081 3012
Size: 2081 931
MspA1I CmG'CkG
Cuts at: 0 9 162 948 1614 3012
Size: 9 153 786 666 1398
Fragments arranged by size:
1398 786 666 153 9
NaeI GCC'GGC
Cuts at: 0 141 2667 3012
Size: 141 2526 345
Fragments arranged by size:
2526 345 141
NcoI C'CATG_G
Cuts at: 0 2515 3012
Size: 2515 497
NdeI CA'TA_TG
Cuts at: 0 2763 3012
Size: 2763 249
NgoMIV G'CCGG_C
Cuts at: 0 139 2665 3012
Size: 139 2526 347
Fragments arranged by size:
2526 347 139
NspI r_CATG'y
Cuts at: 0 2076 2366 3012
Size: 2076 290 646
Fragments arranged by size:
2076 646 290
PflMI CCAn_nnn'nTGG
Cuts at: 0 2950 3012
Size: 2950 62
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 644 676 3012
Size: 644 32 2336
Fragments arranged by size:
2336 644 32
PpuMI rG'GwC_Cy
Cuts at: 0 1218 3012
Size: 1218 1794
PstI C_TGCA'G
Cuts at: 0 1175 3012
Size: 1175 1837
PvuII CAG'CTG
Cuts at: 0 9 948 1614 3012
Size: 9 939 666 1398
Fragments arranged by size:
1398 939 666 9
RsrII CG'GwC_CG
Cuts at: 0 66 3012
Size: 66 2946
SacII CC_GC'GG
Cuts at: 0 163 3012
Size: 163 2849
ScaI AGT'ACT
Cuts at: 0 1130 3012
Size: 1130 1882
SfcI C'TryA_G
Cuts at: 0 204 879 1171 1439 1515 1938 2490 3012
Size: 204 675 292 268 76 423 552 522
Fragments arranged by size:
675 552 522 423 292 268 204 76
SmlI C'TyrA_G
Cuts at: 0 736 3012
Size: 736 2276
SspI AAT'ATT
Cuts at: 0 856 1959 3012
Size: 856 1103 1053
Fragments arranged by size:
1103 1053 856
StyI C'CwwG_G
Cuts at: 0 1671 2036 2515 3012
Size: 1671 365 479 497
Fragments arranged by size:
1671 497 479 365
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1401 1442 3012
Size: 1401 41 1570
Fragments arranged by size:
1570 1401 41
TatI w'GTAC_w
Cuts at: 0 11 374 1128 1496 2414 3012
Size: 11 363 754 368 918 598
Fragments arranged by size:
918 754 598 368 363 11
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2207 2239 3012
Size: 2207 32 773
Fragments arranged by size:
2207 773 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2043 3012
Size: 2043 969
XmnI GAAnn'nnTTC
Cuts at: 0 855 1364 2926 3012
Size: 855 509 1562 86
Fragments arranged by size:
1562 855 509 86
Enzymes that do cut:
AarI AccI AclI AcuI AgeI AloI AlwNI ApoI
AseI BaeI BanI BbsI BbvCI BciVI BglII BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI
BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstXI BstYI
BtgI BtsI ClaI CspCI DraI DrdI EaeI EarI
Eco57MI EcoNI EcoO109I EcoRI FalI FseI Hin4I MfeI
MmeI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI
PflMI PpiI PpuMI PstI PvuII RsrII SacII ScaI
SfcI SmlI SspI StyI TaqII TatI TstI XcmI
XmnI
Enzymes that do not cut:
AatII Acc65I AfeI AflII AflIII AhdI AleI AlfI
ApaI ApaLI AscI AsiSI AvaI AvrII BamHI BanII
BbeI BcgI BclI BfrBI BglI BmrI BmtI BsaBI
BsaHI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BssHII
BstBI BstEII BstZ17I Bsu36I BtgZI DraIII EagI EciI
EcoICRI EcoRV FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MluI MscI NarI NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PshAI
PsiI PspOMI PspXI PsrI PvuI SacI SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI
XhoI XmaI ZraI