Restriction map for
pFN21AE6705
(Linear) (Six-base) MAPSORT of: KIEE6705.seq Check: 4593 from: 1 to: 2301
KIEE6705.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2244 2301
Size: 2244 57
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1267 2301
Size: 1267 1034
AflIII A'CryG_T
Cuts at: 0 65 2301
Size: 65 2236
AleI CACnn'nnGTG
Cuts at: 0 799 2301
Size: 799 1502
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 692 709 2301
Size: 692 17 1592
Fragments arranged by size:
1592 692 17
AlwNI CAG_nnn'CTG
Cuts at: 0 1839 2297 2301
Size: 1839 458 4
Fragments arranged by size:
1839 458 4
ApaI G_GGCC'C
Cuts at: 0 1625 2301
Size: 1625 676
ApoI r'AATT_y
Cuts at: 0 1943 2087 2301
Size: 1943 144 214
Fragments arranged by size:
1943 214 144
AvaI C'yCGr_G
Cuts at: 0 1238 2032 2301
Size: 1238 794 269
Fragments arranged by size:
1238 794 269
BanI G'GyrC_C
Cuts at: 0 2064 2301
Size: 2064 237
BanII G_rGCy'C
Cuts at: 0 1625 2301
Size: 1625 676
BbsI GAAGACnn'nnnn_
Cuts at: 0 460 555 1700 2301
Size: 460 95 1145 601
Fragments arranged by size:
1145 601 460 95
BciVI GTATCCnnnnn_n'
Cuts at: 0 1950 2301
Size: 1950 351
BclI T'GATC_A
Cuts at: 0 497 1062 2301
Size: 497 565 1239
Fragments arranged by size:
1239 565 497
BglII A'GATC_T
Cuts at: 0 1762 2301
Size: 1762 539
BlpI GC'TnA_GC
Cuts at: 0 532 2301
Size: 532 1769
Bme1580I G_kGCm'C
Cuts at: 0 838 1059 1625 2301
Size: 838 221 566 676
Fragments arranged by size:
838 676 566 221
BmrI ACTGGGnnnn_n'
Cuts at: 0 330 1839 1995 2301
Size: 330 1509 156 306
Fragments arranged by size:
1509 330 306 156
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1781 2301
Size: 1781 520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 106 269 436 461 850 896 1127
Size: 34 72 163 167 25 389 46 231
Cuts at: 1127 1452 2240 2274 2301
Size: 325 788 34 27
Fragments arranged by size:
788 389 325 231 167 163 72 46
34 34 27 25
Bpu10I CC'TnA_GC
Cuts at: 0 1534 2301
Size: 1534 767
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2066 2301
Size: 2066 235
BsaI GGTCTCn'nnnn_
Cuts at: 0 774 1984 2301
Size: 774 1210 317
Fragments arranged by size:
1210 774 317
BsaAI yAC'GTr
Cuts at: 0 357 1116 2301
Size: 357 759 1185
Fragments arranged by size:
1185 759 357
BsaWI w'CCGG_w
Cuts at: 0 1634 2301
Size: 1634 667
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1072 1100 1102 1130 1867 1897 2301
Size: 1072 28 2 28 737 30 404
Fragments arranged by size:
1072 737 404 30 28 28 2
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1448 1630 2301
Size: 1448 182 671
Fragments arranged by size:
1448 671 182
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 638 2301
Size: 538 100 1663
Fragments arranged by size:
1663 538 100
BsiHKAI G_wGCw'C
Cuts at: 0 375 671 1634 2226 2301
Size: 375 296 963 592 75
Fragments arranged by size:
963 592 375 296 75
BsmI GAATG_Cn'
Cuts at: 0 1928 2301
Size: 1928 373
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1473 2301
Size: 1473 828
Bsp1286I G_dGCh'C
Cuts at: 0 375 671 838 1059 1625 1634 2226 2301
Size: 375 296 167 221 566 9 592 75
Fragments arranged by size:
592 566 375 296 221 167 75 9
BspHI T'CATG_A
Cuts at: 0 525 974 1856 2301
Size: 525 449 882 445
Fragments arranged by size:
882 525 449 445
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 327 510 660 2244 2301
Size: 327 183 150 1584 57
Fragments arranged by size:
1584 327 183 150 57
BsrBI CCG'CTC
Cuts at: 0 1606 2301
Size: 1606 695
BsrDI GCAATG_nn'
Cuts at: 0 812 2301
Size: 812 1489
BsrFI r'CCGG_y
Cuts at: 0 80 2301
Size: 80 2221
BsrGI T'GTAC_A
Cuts at: 0 159 1521 2301
Size: 159 1362 780
Fragments arranged by size:
1362 780 159
BssSI C'ACGA_G
Cuts at: 0 1332 2301
Size: 1332 969
BstAPI GCAn_nnn'nTGC
Cuts at: 0 652 1213 2058 2301
Size: 652 561 845 243
Fragments arranged by size:
845 652 561 243
BstXI CCAn_nnnn'nTGG
Cuts at: 0 84 801 898 2301
Size: 84 717 97 1403
Fragments arranged by size:
1403 717 97 84
BstYI r'GATC_y
Cuts at: 0 1359 1762 2301
Size: 1359 403 539
Fragments arranged by size:
1359 539 403
BtgI C'CryG_G
Cuts at: 0 757 2301
Size: 757 1544
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1485 2301
Size: 1485 816
BtsI GCAGTG_nn'
Cuts at: 0 1032 1580 2301
Size: 1032 548 721
Fragments arranged by size:
1032 721 548
EaeI y'GGCC_r
Cuts at: 0 760 2301
Size: 760 1541
EarI CTCTTCn'nnn_
Cuts at: 0 772 1153 2301
Size: 772 381 1148
Fragments arranged by size:
1148 772 381
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 106 269 436 461 850 896 1127
Size: 34 72 163 167 25 389 46 231
Cuts at: 1127 1267 1452 2240 2274 2301
Size: 140 185 788 34 27
Fragments arranged by size:
788 389 231 185 167 163 140 72
46 34 34 27 25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 890 2059 2301
Size: 890 1169 242
Fragments arranged by size:
1169 890 242
EcoO109I rG'GnC_Cy
Cuts at: 0 753 880 895 1621 1836 2301
Size: 753 127 15 726 215 465
Fragments arranged by size:
753 726 465 215 127 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 445 2301
Size: 445 1856
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 845 877 1351 1383 1424 1456 1640 1672
Size: 845 32 474 32 41 32 184 32
Cuts at: 1672 1789 1821 2213 2245 2301
Size: 117 32 392 32 56
Fragments arranged by size:
845 474 392 184 117 56 41 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 405 2301
Size: 405 1896
MfeI C'AATT_G
Cuts at: 0 347 2301
Size: 347 1954
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 891 1683 2301
Size: 40 851 792 618
Fragments arranged by size:
851 792 618 40
MscI TGG'CCA
Cuts at: 0 762 2301
Size: 762 1539
MslI CAynn'nnrTG
Cuts at: 0 634 799 1489 2301
Size: 634 165 690 812
Fragments arranged by size:
812 690 634 165
MspA1I CmG'CkG
Cuts at: 0 1021 2301
Size: 1021 1280
NaeI GCC'GGC
Cuts at: 0 82 2301
Size: 82 2219
NcoI C'CATG_G
Cuts at: 0 757 2301
Size: 757 1544
NgoMIV G'CCGG_C
Cuts at: 0 80 2301
Size: 80 2221
NspI r_CATG'y
Cuts at: 0 413 2031 2301
Size: 413 1618 270
Fragments arranged by size:
1618 413 270
PflMI CCAn_nnn'nTGG
Cuts at: 0 292 2301
Size: 292 2009
PpuMI rG'GwC_Cy
Cuts at: 0 753 880 1836 2301
Size: 753 127 956 465
Fragments arranged by size:
956 753 465 127
PspOMI G'GGCC_C
Cuts at: 0 1621 2301
Size: 1621 680
PstI C_TGCA'G
Cuts at: 0 455 698 2063 2255 2301
Size: 455 243 1365 192 46
Fragments arranged by size:
1365 455 243 192 46
PvuII CAG'CTG
Cuts at: 0 1021 2301
Size: 1021 1280
SanDI GG'GwC_CC
Cuts at: 0 753 2301
Size: 753 1548
SbfI CC_TGCA'GG
Cuts at: 0 2255 2301
Size: 2255 46
ScaI AGT'ACT
Cuts at: 0 1910 2301
Size: 1910 391
SfcI C'TryA_G
Cuts at: 0 451 694 745 2059 2251 2301
Size: 451 243 51 1314 192 50
Fragments arranged by size:
1314 451 243 192 51 50
SmlI C'TyrA_G
Cuts at: 0 2081 2301
Size: 2081 220
StyI C'CwwG_G
Cuts at: 0 575 757 891 1422 2301
Size: 575 182 134 531 879
Fragments arranged by size:
879 575 531 182 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1489 1661 2241 2301
Size: 1489 172 580 60
Fragments arranged by size:
1489 580 172 60
TatI w'GTAC_w
Cuts at: 0 159 1521 1908 2301
Size: 159 1362 387 393
Fragments arranged by size:
1362 393 387 159
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 500 532 1072 1100 1104 1132 1286 1318
Size: 500 32 540 28 4 28 154 32
Cuts at: 1318 1335 1367 2301
Size: 17 32 934
Fragments arranged by size:
934 540 500 154 32 32 32 28
28 17 4
Tth111I GACn'n_nGTC
Cuts at: 0 1664 1845 2301
Size: 1664 181 456
Fragments arranged by size:
1664 456 181
Enzymes that do cut:
AarI AcuI AflIII AleI AlfI AlwNI ApaI ApoI
AvaI BanI BanII BbsI BciVI BclI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaWI BsaXI BseRI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI
BstXI BstYI BtgI BtgZI BtsI EaeI EarI Eco57MI
EcoNI EcoO109I FalI Hin4I HincII MfeI MmeI MscI
MslI MspA1I NaeI NcoI NgoMIV NspI PflMI PpuMI
PspOMI PstI PvuII SanDI SbfI ScaI SfcI SmlI
StyI TaqII TatI TstI Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbeI BbvCI BcgI BfrBI BglI BmgBI BmtI BsaBI
BsaHI BseYI BsiEI BsiWI BspEI BssHII BstBI BstEII
BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MluI NarI NdeI NheI
NotI NruI NsiI PacI PasI PciI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SacII SalI SapI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI XbaI XcmI XhoI XmaI XmnI ZraI