Restriction map for
pFN21AE6703
(Linear) (Six-base) MAPSORT of: KIEE6703.seq Check: 7308 from: 1 to: 2646
KIEE6703.orf
With 182 enzymes: SgfI *
March 16, 2015 11:35 ..
AclI AA'CG_TT
Cuts at: 0 2331 2646
Size: 2331 315
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1028 1787 2250 2646
Size: 1028 759 463 396
Fragments arranged by size:
1028 759 463 396
AleI CACnn'nnGTG
Cuts at: 0 703 2646
Size: 703 1943
AlwNI CAG_nnn'CTG
Cuts at: 0 1240 2646
Size: 1240 1406
ApaI G_GGCC'C
Cuts at: 0 2289 2646
Size: 2289 357
ApaLI G'TGCA_C
Cuts at: 0 706 2646
Size: 706 1940
ApoI r'AATT_y
Cuts at: 0 302 506 778 842 851 1795 1853 2089
Size: 302 204 272 64 9 944 58 236
Cuts at: 2089 2646
Size: 557
Fragments arranged by size:
944 557 302 272 236 204 64 58
9
AseI AT'TA_AT
Cuts at: 0 2245 2646
Size: 2245 401
AvaI C'yCGr_G
Cuts at: 0 398 2646
Size: 398 2248
BamHI G'GATC_C
Cuts at: 0 600 2068 2646
Size: 600 1468 578
Fragments arranged by size:
1468 600 578
BanI G'GyrC_C
Cuts at: 0 186 1576 2098 2646
Size: 186 1390 522 548
Fragments arranged by size:
1390 548 522 186
BanII G_rGCy'C
Cuts at: 0 2289 2646
Size: 2289 357
BbeI G_GCGC'C
Cuts at: 0 2102 2646
Size: 2102 544
BciVI GTATCCnnnnn_n'
Cuts at: 0 2218 2314 2646
Size: 2218 96 332
Fragments arranged by size:
2218 332 96
BglII A'GATC_T
Cuts at: 0 2525 2646
Size: 2525 121
Bme1580I G_kGCm'C
Cuts at: 0 710 2289 2646
Size: 710 1579 357
Fragments arranged by size:
1579 710 357
BmrI ACTGGGnnnn_n'
Cuts at: 0 824 1186 2284 2646
Size: 824 362 1098 362
Fragments arranged by size:
1098 824 362 362
BmtI G_CTAG'C
Cuts at: 0 721 2646
Size: 721 1925
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2245 2646
Size: 2245 401
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 770 1652 1832 2646
Size: 144 626 882 180 814
Fragments arranged by size:
882 814 626 180 144
Bpu10I CC'TnA_GC
Cuts at: 0 182 2646
Size: 182 2464
BsaAI yAC'GTr
Cuts at: 0 516 1611 1623 2623 2646
Size: 516 1095 12 1000 23
Fragments arranged by size:
1095 1000 516 23 12
BsaBI GATnn'nnATC
Cuts at: 0 1294 2646
Size: 1294 1352
BsaHI Gr'CG_yC
Cuts at: 0 1252 2099 2646
Size: 1252 847 547
Fragments arranged by size:
1252 847 547
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 1495 1835 2646
Size: 76 1419 340 811
Fragments arranged by size:
1419 811 340 76
BseYI C'CCAG_C
Cuts at: 0 27 2281 2646
Size: 27 2254 365
Fragments arranged by size:
2254 365 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1993 2646
Size: 1993 653
BsiHKAI G_wGCw'C
Cuts at: 0 710 2646
Size: 710 1936
BsmI GAATG_Cn'
Cuts at: 0 1369 2646
Size: 1369 1277
Bsp1286I G_dGCh'C
Cuts at: 0 710 2289 2646
Size: 710 1579 357
Fragments arranged by size:
1579 710 357
BspHI T'CATG_A
Cuts at: 0 1911 2646
Size: 1911 735
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1813 2646
Size: 1813 833
BsrBI CCG'CTC
Cuts at: 0 114 2646
Size: 114 2532
BsrDI GCAATG_nn'
Cuts at: 0 17 2646
Size: 17 2629
BsrFI r'CCGG_y
Cuts at: 0 496 1520 2646
Size: 496 1024 1126
Fragments arranged by size:
1126 1024 496
BstAPI GCAn_nnn'nTGC
Cuts at: 0 2592 2646
Size: 2592 54
BstEII G'GTnAC_C
Cuts at: 0 2341 2646
Size: 2341 305
BstXI CCAn_nnnn'nTGG
Cuts at: 0 475 570 1812 2646
Size: 475 95 1242 834
Fragments arranged by size:
1242 834 475 95
BstYI r'GATC_y
Cuts at: 0 360 600 1528 2068 2303 2377 2525 2646
Size: 360 240 928 540 235 74 148 121
Fragments arranged by size:
928 540 360 240 235 148 121 74
Bsu36I CC'TnA_GG
Cuts at: 0 1106 1967 2534 2646
Size: 1106 861 567 112
Fragments arranged by size:
1106 861 567 112
BtgI C'CryG_G
Cuts at: 0 1656 2205 2646
Size: 1656 549 441
Fragments arranged by size:
1656 549 441
DraI TTT'AAA
Cuts at: 0 1409 1995 2106 2646
Size: 1409 586 111 540
Fragments arranged by size:
1409 586 540 111
DraIII CAC_nnn'GTG
Cuts at: 0 1738 1740 2300 2646
Size: 1738 2 560 346
Fragments arranged by size:
1738 560 346 2
EaeI y'GGCC_r
Cuts at: 0 1518 2384 2646
Size: 1518 866 262
Fragments arranged by size:
1518 866 262
EarI CTCTTCn'nnn_
Cuts at: 0 205 1328 1835 2646
Size: 205 1123 507 811
Fragments arranged by size:
1123 811 507 205
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 144 770 1028 1652 1787 1832 2250 2646
Size: 144 626 258 624 135 45 418 396
Fragments arranged by size:
626 624 418 396 258 144 135 45
EcoNI CCTnn'n_nnAGG
Cuts at: 0 950 2646
Size: 950 1696
EcoO109I rG'GnC_Cy
Cuts at: 0 1825 2285 2286 2646
Size: 1825 460 1 360
Fragments arranged by size:
1825 460 360 1
EcoRI G'AATT_C
Cuts at: 0 851 2646
Size: 851 1795
EcoRV GAT'ATC
Cuts at: 0 665 2262 2646
Size: 665 1597 384
Fragments arranged by size:
1597 665 384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2629 2646
Size: 2629 17
HaeII r_GCGC'y
Cuts at: 0 2102 2646
Size: 2102 544
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 204 236 314 346 727 759 1520 1552
Size: 204 32 78 32 381 32 761 32
Cuts at: 1552 2517 2549 2646
Size: 965 32 97
Fragments arranged by size:
965 761 381 204 97 78 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 2098 2646
Size: 2098 548
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 423 893 2646
Size: 423 470 1753
Fragments arranged by size:
1753 470 423
MscI TGG'CCA
Cuts at: 0 2386 2646
Size: 2386 260
MslI CAynn'nnrTG
Cuts at: 0 205 595 703 2646
Size: 205 390 108 1943
Fragments arranged by size:
1943 390 205 108
MspA1I CmG'CkG
Cuts at: 0 27 748 2646
Size: 27 721 1898
Fragments arranged by size:
1898 721 27
NaeI GCC'GGC
Cuts at: 0 1522 2646
Size: 1522 1124
NarI GG'CG_CC
Cuts at: 0 2099 2646
Size: 2099 547
NdeI CA'TA_TG
Cuts at: 0 2514 2646
Size: 2514 132
NgoMIV G'CCGG_C
Cuts at: 0 1520 2646
Size: 1520 1126
NheI G'CTAG_C
Cuts at: 0 717 2646
Size: 717 1929
PfoI T'CCnGG_A
Cuts at: 0 121 1629 2444 2646
Size: 121 1508 815 202
Fragments arranged by size:
1508 815 202 121
PpuMI rG'GwC_Cy
Cuts at: 0 1825 2646
Size: 1825 821
PsiI TTA'TAA
Cuts at: 0 1705 2646
Size: 1705 941
PspOMI G'GGCC_C
Cuts at: 0 2285 2646
Size: 2285 361
PvuII CAG'CTG
Cuts at: 0 27 748 2646
Size: 27 721 1898
Fragments arranged by size:
1898 721 27
ScaI AGT'ACT
Cuts at: 0 1872 2646
Size: 1872 774
SfcI C'TryA_G
Cuts at: 0 1156 1918 2646
Size: 1156 762 728
Fragments arranged by size:
1156 762 728
SfoI GGC'GCC
Cuts at: 0 2100 2646
Size: 2100 546
SmlI C'TyrA_G
Cuts at: 0 398 2646
Size: 398 2248
SnaBI TAC'GTA
Cuts at: 0 1611 1623 2646
Size: 1611 12 1023
Fragments arranged by size:
1611 1023 12
SpeI A'CTAG_T
Cuts at: 0 577 2646
Size: 577 2069
SspI AAT'ATT
Cuts at: 0 69 2646
Size: 69 2577
StyI C'CwwG_G
Cuts at: 0 468 2613 2646
Size: 468 2145 33
Fragments arranged by size:
2145 468 33
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 19 2646
Size: 19 2627
TatI w'GTAC_w
Cuts at: 0 1870 2646
Size: 1870 776
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1726 1758 2197 2229 2646
Size: 1726 32 439 32 417
Fragments arranged by size:
1726 439 417 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 594 2297 2646
Size: 594 1703 349
Fragments arranged by size:
1703 594 349
XhoI C'TCGA_G
Cuts at: 0 398 2646
Size: 398 2248
XmnI GAAnn'nnTTC
Cuts at: 0 393 450 890 1043 2646
Size: 393 57 440 153 1603
Fragments arranged by size:
1603 440 393 153 57
Enzymes that do cut:
AclI AcuI AleI AlwNI ApaI ApaLI ApoI AseI
AvaI BamHI BanI BanII BbeI BciVI BglII Bme1580I
BmrI BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaHI
BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI
BsrBI BsrDI BsrFI BstAPI BstEII BstXI BstYI Bsu36I
BtgI DraI DraIII EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NaeI NarI NdeI NgoMIV NheI PfoI
PpuMI PsiI PspOMI PvuII ScaI SfcI SfoI SmlI
SnaBI SpeI SspI StyI TaqII TatI TstI XcmI
XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AlfI AloI AscI AsiSI AvrII BaeI BbsI
BbvCI BcgI BclI BfrBI BglI BlpI BmgBI BpuEI
BsaI BsaWI BsaXI BsiEI BsiWI BsmBI BspEI BsrGI
BssHII BssSI BstBI BstZ17I BtgZI BtsI ClaI CspCI
DrdI EagI EciI EcoICRI FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI NcoI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PshAI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI SexAI SfiI SgfI
SgrAI SmaI SphI SrfI StuI SwaI Tth111I XbaI
XmaI ZraI